Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
권호 표지

The Experimental Model Development of Antibiotic Resistance Gene Transfer Characteristics with Various Micropollutants

미량오염물질에 의한 항생제 내성 유전자 전이 특성에 대한 실험모델 개발

  • Kim, Doocheol (Department of Urban Planning Research, Daejeon Development Institute) ;
  • Oh, Junsik (Department of Environmental Engineering, Korea University) ;
  • Kim, Sungpyo (Department of Environmental Engineering, Korea University)
  • Published : 2012.11.30
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Abstract

Recently, antibiotic resistant genes (ARGs) in the environment are emerging as pollutants, since these genetic contaminants can eventually be transferred to human pathogens. The aim of this study was to develop the experimental model of antibiotic resistant gene (ARG) plasmid transfer as a function of various environmental conditions. For this purpose, the multi drug resistant plasmid pB10, which is known to be originally isolated from a wastewater treatment plant, was selected as a model transfer plasmid and Escherichia coli $DH5{\alpha}$ containing pB10 was used as a model donor. Pseudomonas aeruginosa, an opportunistic pathogen, was selected as the recipient for the conjugation experiment. When the donor and recipient were exposed to various stressors including antibiotics and heavy metal as a function of the concentrations (10, 100 and, 1000 ppb), statistically increased plasmid transfer rate was observed at a concentration of 10 ppb of tetracycline and sulfamethoxazole compared to control (no antibiotic exposure). Accordingly, the developed experimental ARG model by various stressor is a promising tool for evaluating the dissemination of ARGs by micro-contaminants in aquatic environment.

Keywords

References

  1. Barr, V., Barr, K., Millar, M. R., and Lacey, R. W. (1986). $\beta-Lactam$ Antibiotics Increase the Frequency of Plasmid Transfer in Staphylococcus Aureus, Antimicrobial Agents and Chemotherapy, 17, pp. 409-413. https://doi.org/10.1093/jac/17.4.409
  2. Batt A. L., Kim S., and Aga D. S. (2006). Enhanced Biodegradation of Iopromide and Trimethoprim in Nitrifying Activated Sludge, Environmental Science & Technology, 40, pp. 7367-7373. https://doi.org/10.1021/es060835v
  3. Bonheyo, G. T., Hund, B. D., Shoemaker, N. B., and Salyers, A. A. (2001). Transfer Region of a Bacteroides Conjugative Transposon Contains Regulatory as Well as Structural Genes, Plasmid, 46, pp. 202-209. https://doi.org/10.1006/plas.2001.1545
  4. Bonot, S. and Merlin, C. (2010). Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR, Applied and Environmental Microbiology, 76, pp. 378-382. https://doi.org/10.1128/AEM.01125-09
  5. Coleman, M. E., Hope, B. K., Claycamp, H. G., and Cohen, J. T. (2007). Microbial Risk Assessment Scenarios, Causality, and Uncertainty, Microbe, 2, pp. 13-17.
  6. Fajardo, A. and Martinez, J. L. (2008). Antibiotics as Signals that Trigger Specific Bacterial Responses, Current Opinion in Microbiology, 11, pp. 161-167. https://doi.org/10.1016/j.mib.2008.02.006
  7. Forbes, B. A. and Schaberg, D. R. (1983). Transfer of Resistance Plasmids from Staphylococcus Epidermidis to Staphylococcus Aureus: Evidence for Conjugative Exchange of Resistance, Journal of Bacteriology, 153, pp. 627-634.
  8. Guardabassi, L. and Dalsgaard, A. (2002). Occurrence and Fate of Antibiotic Resistant Bacteria in Sewage, Environmental Project Report No. 722. Danish Environmental Protection Agency.
  9. Guardabassi, L., Lo Fo Wong, D. M., and Dalsgaard, A. (2002). The Effects of Tertiary Wastewater Treatment on the Prevalence of Antimicrobial Resistant Bacteria, Water Research, 36, pp. 1955-1964. https://doi.org/10.1016/S0043-1354(01)00429-8
  10. Jung, H. M,, O, S. H., and Park, J. H. (2003). Seasonal Variation of Total Culturable Viruses and Indicator Bacteria in Drinking Water, Journal of Korean Society on Water Environment, 19(3), pp. 329-338. [Korean Literature]
  11. Kim, E. S., Sim, K. B., Kim, S. D., and Choi, H. I. (2012). Water Quality Assessment for Reservoirs using the Korean Trophic State Index, Journal of Korean Society on Water Environment, 28(1), pp. 78-83. [Korean Literature]
  12. Kim, M. A., Kim, Y. H., Lee, H. K., Hwang, D. H., and Kim, J. Y. (2004). Estimation of Nutrients Reduction Rates to Prevent Eutrophication on the Hwaong Reservoir, Journal of Korean Society on Water Environment, 20(6), pp. 589-596. [Korean Literature]
  13. Kim, S. (2005). The Effect of Operational Parameters on the Fate of Tetracycline Resistant Bacteria in Biological Wastewater Treatment Plants, Ph.D. Dissertation, State University of New York at Buffalo.
  14. Knapp, C., Andolfing, J., Ehlert, P. I., and Graham D. W. (2010). Evidence of Increasing Antibiotic Resistance Gene Abundances in Archived Soils Since 1940, Environmental Science & Technology, 44, pp. 580-587. https://doi.org/10.1021/es901221x
  15. Levy, S. B. (2002). Factors Impacting on the Problem of Antibiotic Resistance, Journal of Antimicrobial Chemotherapy, 49, pp. 25-30. https://doi.org/10.1093/jac/49.1.25
  16. Livermore, D., Pillay, D., and Cane, P. (2005). Antimicrobial Resistance; Inevitable, but not Unmanageable, Health Protection Agency (HPA), pp. 4-11.
  17. Oh, H. K., Lee, J. J., Lee, J. Y., and Park, J. H. (2007). Development of a Cultivation Method for Detecting Antibiotic Resistant Microorganisms from Various Water Samples, Korean Society of Civil Engineers, 27, pp. 711-717. [Korean Literature]
  18. Ohlsen, K., Ternes, T., Werner, G., Wallner, U., Loffler, D., Ziebuhr, W., Witte, W., and Hacker, J. (2003). Impact of Antibiotics on Conjugational Resistance Gene Transfer in Staphylococcus Aureus in Sewage, Environmental Microbiology, 5, pp. 711-716. https://doi.org/10.1046/j.1462-2920.2003.00459.x
  19. Park. J. H. (2008). Hwan-kyung Ne Hang-saeng-je Ne-sung Se-gyun Mit Yu-hae-sung-ye Kwan-han Kuk-ne Cho-sa Hyun-hwang, Infection and Chemotherapy, 40, pp. 150-157. [Korean Literature]
  20. Reinthaler, F. F., Posch, J., Feierl, G., Wust, G., and Haas, D., Ruckenbauer, G., Mascher, F., Marth, E. (2003). Antibiotic Resistance of E.coli in Sewage and Sludge, Water Research, 37, pp. 1685-1690. https://doi.org/10.1016/S0043-1354(02)00569-9
  21. Sambrook, J. and Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press, New York.
  22. Satoshi, S., Hidetaka, O., Daisuke, I., Hirofumi, T., Kazunari, S., and Michihiko, I. (2008). Transfer of Antibiotic Multi Resistant Plasmid RP4 from Escherichia coli to Activated Sludge Bacteria, Journal of Bioscience and Bioengineering, 106, pp. 292-296. https://doi.org/10.1263/jbb.106.292
  23. Schluter, A., Heuer, H., Szczepanowski, R., Forney, L. j., Thomas, C. M., Puhler, A., and Top, E. M. (2003). The 64508 bp $IncP-1{\beta}$ Antibiotic Multi Resistance Plasmid pB10 Isolated from a Waste Water Treatment Plant Provides Evidence for Recombination between Members of Different Branches of the $IncP-1{\beta}$ Group, Microbiology, 149, pp. 3139-3153. https://doi.org/10.1099/mic.0.26570-0