• IE interfaces
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• v.6 no.2
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• pp.19-35
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• 1993

This paper presents the development of a knowledge-based expert system to assist for designer in the process of designing products for easier assembly. The developed expert system for supporting assembly-oriented product design, which is named as DFA-MASTER, consists of two subsystems such as "Assembly Method Selection System" which provides feasible assembly methods to designer and "DFA-Analysis System" which provides guidelines to improve assemblability of products in the early stage of product design. The developed system is interfaced with AutoCAD system and can be run under PC environments. By using DFA-MASTER, designer can obtain feasible assembly methods which have high assemblability under the given asssembly conditions and also evaluate the assemblability of part feature or product structure.

• Proceedings of the Korea Information Processing Society Conference
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• 2011.04a
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• pp.11-13
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• 2011

최근 스마트 폰, 타블렛 PC 등 고성능 모바일 기기에 대한 시장수요가 증가함에 따라 임베디드 프로세서에 대한 성능 최적화가 활발히 이루어지고 있다. 고성능 멀티미디어 시스템을 대상으로 설계된 Empress 프로세서는 다수의 기능 유닛을 포함하고 있어 명령어 스케줄링을 통해 성능 향상을 기대할 수 있으나 기존의 컴파일러는 이를 지원하지 않고 있다. 본 연구에서는 GNU C 컴파일러를 이용하여 Empress 프로세서를 위한 DFA 기반의 명령어 스케줄링 최적화를 구현하였다. 그 결과 EEMBC 벤치마크를 이용한 성능 분석에서 실행시간 기준 평균 8%의 향상이 있음을 확인하였다.

• Journal of Korea Foundry Society
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• v.43 no.6
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• pp.325-329
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• 2023

높은 주조 품질을 요구받고 있는 대부분의 다이캐스팅업체들은 양산공정을 통해 사용되고있는 용탕의 청정도를 평가하고 모니터링을 해야만 한다. 여러 가지 평가방법들을 사용할 수 있지만 현장에서는 일부 측정방법만이 다이캐스팅에 적용되고 있으며 목적에 맞게 적절하게 사용하여야 한다. 세 가지 가능한 평가 방법인 K-Mold, 기계적물성 평가 및 PoDFA(다공성 디스크 여과 장치) 를 대상으로 소개하고자 한다. 우선, 각 방법들은 서로 사용 목적 목적에 따라 선택될 수 있으며 K-Mold는 현장에서 빠르고 간단하게 적용할 수 있는 방법으로 일정한 규격화된 시편의 파면을 만들고 파단면 관찰을 통해 일정한 크기 이상의 산화물 갯수를 확인하여 용탕의 품질을 관리하는 편리한 평가방법 중 하나다. 기계적 물성 테스트는 용탕 청결도에 대한 정성적, 정량적 정보를 모두 확인할 수 있으며 샘플링 테스트에 적합한 시험법이라 할 수 있다. 또한, PoDFA는 용탕내에 개재물의 종류별 정밀분석과 양에 대한 상대적 비교 분석으로써 현장의 많은 경험을 통하여 데이터 축적하고 분석하는 방법으로 사용방법에 대한 전문교육이 필요하다.

• Journal of Life Science
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• v.19 no.9
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• pp.1218-1225
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• 2009

Difractose anhydrides (DFAs) is studied as a sweetener for diabetics because of its structural property. DFAs have four types: DFA I, III, IV (degradation of levan) and V (degradation of inulin). Especially, DFA IV has been shown to enhance the absorption of calcium in experiments using rats. Levan fructotransferase is an enzyme for producing di-d-fructose-2,6':6,2-dianhydride (DFA IV). To identify structural characterization, we purified wild-type and mutants (D63A, D195N and N85S) of levan fructotransferase (LFTase) from Microbacterium sp. AL-210. These proteins were purified to apparent homogeneity by Ni-NTA affinity column, Q-sepharose ion exchange and gel filtration chromatography and detected by SDS-PAGE. They were also analyzed by circular dichroism (CD) measurements, JNET secondary structure prediction, activity measurements at various temperatures, and pH analysis. The optimum pH for the enzyme-catalyzed reaction was pH 7.5 and optimum temperature was observed at $55^{\circ}C$. Along with wild-type LFTase, mutants were analyzed by CD measurement, fluorescence analysis and differential scanning calorimetry (DSC). N85S showed less $\alpha$-helix and more $\beta$ strand than others. Also, N85S showed almost the same curve as wild-type in their steady-state fluorescence spectra, whereas mutant D63A and D195N showed higher intensity than wild-type. The amino acid sequence of wild-type LFTase was compared to the sequences of exo-inulinase from Aspergillus awamori, a plant fructan 1-exohydrolase from Cichorium intybus, and Thermotogo maritime (Tm) invertase and showed a high identity with Exo-inulinase from Aspergillus awamori.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.37-43
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• 2007

A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2': 2,3' dianhydride [DFA III]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA III-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography. The purified enzyme showed much higher specific activity (1,276 units/mg protein) than other DFA III-producing IFTases. The recombinant and native enzymes were optimally active in very similar pH and temperature conditions. With a 103-min half-life at $60^{\circ}C$, the recombinant enzyme was as stable as the native enzyme. Acidic residues and cysteines potentially involved in the catalytic mechanism are proposed based on an alignment with other IFTases and a DFA IIIase.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.1
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• pp.91-97
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• 2017

Hyperglycemia is associated with an increased risk of cardiovascular diseases. It has been demonstrated that chronic exposure to high glucose impaired endothelial functions. However, specific effects of short-term exposure to high glucose on vascular reactivity are controversial. Moreover, the combined effects of other metabolic substrates such as free fatty acids (FFA) on vascular reactivity remain poorly understood. Here we investigate the effects of short-term exposure to high glucose with or without other metabolic substrates including FFAs termed "nutrition full" (NF) solution, on mesenteric (MA) and deep femoral arteries (DFA) of rats. Arterial ring segments were mounted in a double-wire myograph. Contraction in response to phenylephrine (PhE) was determined in control (5 mM) and high glucose (23 mM, HG) environments over a 30 min period. In both arteries, PhE-inducedvasocontraction was enhanced by pre-incubation of HG solution. A combined incubation with HG and palmitic acid ($100{\mu}M$) induced similar sensitization of PhE-contractions in both arteries. In contrast, high $K^+$-induced contractions were not affected by HG. Interestingly, pre-incubation with NF solution decreased PhE-induced contraction in MA but increased the contraction in DFA. In NF solution, the HG-induced facilitation of PhE-contraction was not observed in MA. Furthermore, the PhE-induced contraction of DFA was attenuated by HG in NF solution. Our results demonstrate that the sensitization of PhE-induced arterial contraction by HG is differentially affected by other metabolic substrates. The conversation of skeletal arterial contractility by HG in NF solution requires careful interpretation of the previous in vitro studies where only glucose is included in physiological salt solutions. Further studies are required to elucidate the mechanism underlying the inconsistent effect of NF solution on MA and DFA.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.23 no.3
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• pp.395-404
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• 2013

A pattern matching algorithm plays an important role in traffic identification and classification based on predefined patterns for intrusion detection and prevention. As attacks become prevalent and complex, current patterns are written using regular expressions, called regexes, which are expressed into the deterministic finite automata(DFA) due to the guaranteed worst-case performance in pattern matching process. Currently, because of the increased complexity of regex patterns and their large number, memory-efficient DFA from states reduction have become the mainstay of pattern matching process. However, most of the previous works have focused on reducing only the number of states on a single automaton, and thus there still exists a state blowup problem under the large number of patterns. To solve the above problem, we propose a new state compression algorithm that merges states on multiple automata. We show that by merging states with the same input character on multiple automata, the proposed algorithm can lead to a significant reduction of the number of states in the original DFA by as much as 40.0% on average.

• Journal of the Korea Society of Computer and Information
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• v.19 no.12
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• pp.133-141
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• 2014

An efficient method for reducing power consumption in pipelined deep packet inspection systems is proposed. It is based on the observation that the number of memory accesses is dominant for the power consumption and the number of accesses drops drastically as the input goes through stages of the pipelined AC-DFA. A DPI system is implemented where the operating frequency of the stages that are not frequently used in the pipeline is reduced to eliminate the waste of power consumption. The power consumption of the proposed DPI system is measured upon various input character set and up to 25% of reduction of total power consumption is obtained, compared to those of the recent DPI systems. The method can be easily applied to other pipelined architecture and string searching applications.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.184-188
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• 1997

A bacterial strain, producing extracellular inulin fructotransferase which converts inulin into di-D-fructofuranose 1,2':2,3' dianhydride(DFA III), was isolated from soil and presumed as Bacillus sp.. The highest production of the enzyme was obtained by using medium containing Jerusalem artichoke extract as carbon source, peptone as organic nitrogen source, and $NH_4H_2PO_4$, as inorganic source. Under optimum condition, the enzyme activity of the culture broth supernatant reached maximal 2.61 units/ml after cultivation for 45 hrs.

• The Korean Journal of Physiology and Pharmacology
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• v.23 no.5
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• pp.403-409
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• 2019

Free fatty acid (FFA) intake regulates blood pressure and vascular reactivity but its direct effect on contractility of systemic arteries is not well understood. We investigated the effects of saturated fatty acid (SFA, palmitic acid), polyunsaturated fatty acid (PUFA, linoleic acid), and monounsaturated fatty acid (MUFA, oleic acid) on the contractility of isolated mesenteric (MA) and deep femoral arteries (DFA) of Sprague-Dawley rats. Isolated MA and DFA were mounted on a dual wire myograph and phenylephrine (PhE, $1-10{\mu}M$) concentration-dependent contraction was obtained with or without FFAs. Incubation with $100{\mu}M$ of palmitic acid significantly increased PhE-induced contraction in both arteries. In MA, treatment with $100{\mu}M$ of linoleic acid decreased $1{\mu}M$ PhE-induced contraction while increasing the response to higher PhE concentrations. In DFA, linoleic acid slightly decreased PhE-induced contraction while $200{\mu}M$ oleic acid significantly decreased it. In MA, oleic acid reduced contraction at low PhE concentration (1 and $2{\mu}M$) while increasing it at $10{\mu}M$ PhE. Perplexingly, depolarization by 40 mM KCl-induced contraction of MA was commonly enhanced by the three fatty acids. The 40 mM KCl-contraction of DFA was also augmented by linoleic and oleic acids while not affected by palmitic acid. SFA persistently increased alpha-adrenergic contraction of systemic arteries whereas PUFA and MUFA attenuated PhE-induced contraction of skeletal arteries. PUFA and MUFA concentration-dependent dual effects on MA suggest differential mechanisms depending on the types of arteries. Further studies are needed to elucidate underlying mechanisms of the various effects of FFA on systemic arteries.