• Korean Journal of Clinical Laboratory Science
• /
• v.51 no.4
• /
• pp.514-520
• /
• 2019

This study investigated the improved lipid metabolism effect of 3T3-L1 cells induced by adipocytes using the dichloromethane (DCM) fraction in the organic solvent extract of Wassong (Orostachys japonicus). To confirm the cell cytotoxicity, each of 6 fractions of organic solvent extracts (EtOH, Hexane, DCM, EtOAc, BuOH, and H₂O) was examined using MTS assay. As a result, it was confirmed that the DCM extract was stable over the whole range of concentrations, and a DCM fraction was used to confirm the improved lipid metabolism effect. Lipid excretion was measured to confirm the change of lipid metabolism. 3T3-L1 cells induced by adipocytes were treated with DCM extract and stained with oil-red O to evaluate lipid accumulation. As a result, it was confirmed that the lipid efflux was significantly improved. In order to confirm the mechanism of lipid efflux, the mRNA expressions of ABCA1 and ABCG1, which are lipid transport proteins, were confirmed by real-time PCR. Therefore, the present study confirmed that the DCM extract from Orostachys japonicus has the effect of improving the lipid metabolism on 3T3-L1 adipocytes. In addition, the results of this study will be used as the basis for the development of functional foods using Orostachys japonicus and also for conducting research on the detailed mechanisms.

• Journal of Industrial Technology
• /
• v.26 no.B
• /
• pp.119-126
• /
• 2006

Capacity spectrum method(CSM) of ATC-40(1996) and displacement coefficient method(DCM)of FEMA-273(1997) are applied to evaluate the seismic performance of bridges. In this study, equivalent response is obtained from nonlinear static analysis for the 3spans continues bridge and nonlinear maximum displacement response is calculated using CSM and DCM. Nonlinear maximum displacement response of DCM is larger than this of CSM. It is method that DCM can evaluate target displacement and ductility of structural to be easy and simple, but tend to overestimate the maximum displacement response. Therefore, this method is mainly used at preparation design level to evaluate the structural response. It is not desirable to evaluate the seismic performance using DCM.

• Biomolecules & Therapeutics
• /
• v.30 no.5
• /
• pp.399-408
• /
• 2022

Diabetic cardiomyopathy (DCM) is described as abnormalities of myocardial structure and function in diabetic patients without other well-established cardiovascular factors. Although multiple pathological mechanisms involving in this unique myocardial disorder, mitochondrial dysfunction may play an important role in its development of DCM. Recently, considerable progresses have suggested that mitochondrial biogenesis is a tightly controlled process initiating mitochondrial generation and maintaining mitochondrial function, appears to be associated with DCM. Nonetheless, an outlook on the mechanisms and clinical relevance of dysfunction in mitochondrial biogenesis among patients with DCM is not completely understood. In this review, hence, we will summarize the role of mitochondrial biogenesis dysfunction in the development of DCM, especially the molecular underlying mechanism concerning the signaling pathways beyond the stimulation and inhibition of mitochondrial biogenesis. Additionally, the evaluations and potential therapeutic strategies regarding mitochondrial biogenesis dysfunction in DCM is also presented.

• Journal of Haehwa Medicine
• /
• v.29 no.2
• /
• pp.22-29
• /
• 2020

Objectives: This study aims to investigate the effects of diabetes mellitus care mixture (DCM) on blood glucose and lipid metabolism in type 2 diabetic mellitus mice. DCM consisted of lagerstroemia speciose, allium hookeri, momordica charantia, amaranthus tricolor, and boesenbergia rotunda, which have been proven to have antidiabetic properties. Methods: In this study, we researched the effects of DCM in type 2 diabetic mellitus mice. C57BLKS/J mouse groups had no treatment, db/db mouse randomly assigned to 2 groups, and treated with distilled water and DCM (200 mg/kg/day). Blood glucose levels and body weight were checked every week. After 4 weeks of treatment, liver function indicators (AST, ALT, and LDH) and lipid metabolites (triglyceride, total cholesterol, LDL-cholesterol, HDL-cholesterol) were measured with a biochemistry analyzer. Diabetic factors (insulin, resistin, and leptin) were measured with ELISA. Results: DCM was decreased blood glucose, diabetic factors, liver function indicators, triglyceride, total cholesterol, and LDL-cholesterol significantly. Also, HDL-cholesterol was significantly increased in DCM group. The bodyweight of DCM group decreased but, no significant difference with the control group. DCM may have the potential to improved diabetes mellitus by regulating blood glucose levels and diabetic factors. Also protecting from diabetic complications by adjusting liver function indicators and lipid metabolites. Conclusions: These results suggest that DCM to be used as an oriental medicine for diabetes, the results of clinical trials are needed.

• Journal of IKEEE
• /
• v.16 no.2
• /
• pp.131-137
• /
• 2012

This paper is study on a new high efficiency DC-DC converter of discontinuous conduction mode (DCM) with zero voltage switching (ZVS). The converters of high efficiency are generally made that the power loss of the used semiconductor switching devices is minimized. The proposed converter is accomplished that the turn-on operation of switches is on zero current switching (ZCS) by DCM. The converter is also applicable to a new quasi-resonant circuit to achieve high efficiency converter. The control switches using in the converter are operated with soft switching, that is, ZVS and ZCS by quasi-resonant method. The control switches are operated without increasing their voltage and current stresses by the soft switching technology. The result is that the switching loss is very low and the efficiency of the converter is high. The soft switching operation and the system efficiency of the proposed DCM-ZVS converter are verified by digital simulation and experimental results.

• Journal of the Korean GEO-environmental Society
• /
• v.23 no.5
• /
• pp.25-32
• /
• 2022

As recently, there have been two earthquakes with a magnitude of 5.0 or greater in Korea and the number of smaller earthquakes has increased, a lot of research and interest in earthquake-resistant design are increasing. Especially, the Pohang earthquake has also raised interest in earthquake-resistant design of port facilities. In this study, experiments and analysis were conducted on the dynamic behavior of upright and inclined breakwaters during earthquakes among port structures through the 1g shaking table test. To this end, three seismic waves were applied to the model to which the similarity law (scale effect) was applied: long period (Hachinohe), short period (Ofunato) and artificial seismic waves. The acceleration and displacement of the upright and inclined breakwaters were analyzed according to whether the DCM method was reinforced during earthquakes based on the results of shaking table test. As the result, the dynamic behavior of the upright and inclined breakwater shows a tendency to suppress the amplification of acceleration as bearing capacity and rigidity increase when DCM method is reinforced.

• Animal Bioscience
• /
• v.36 no.6
• /
• pp.953-961
• /
• 2023

Objective: This study aimed to investigate the effect of dorsal cranial myopathy (DCM) on chicken meat quality. Methods: Sixty-six Ross 308 AP broilers, 47 days old, of both sexes, weighing about 3.51 kg, were slaughtered according to standard industrial practices, and evaluated for meat color, pH, chemical composition, collagen content, fatty acid profile, and histopathological parameters. Comparisons between normal and DCM-affected meat were performed using Student's t-test at the 5% significance level. Results: Histological analysis of muscle tissues affected by DCM showed myofiber degeneration, proliferation of inflammatory cells, fibroplasia, and necrosis with fibrosis. DCM samples had lower protein content and higher moisture, ash, insoluble collagen, total collagen, and pH. DCM-affected meat was redder and more yellowish. There were no differences in lipid or soluble collagen contents between groups. DCM-affected meat had higher percentages of arachidonic acid (C20:4n-6) and eicosapentaenoic acid (C20:5n-3). Conclusion: This study revealed that DCM-affected meat exhibits considerable changes in quality parameters.

• Proceedings of the KIPE Conference
• /
• 2011.07a
• /
• pp.165-166
• /
• 2011

본 논문에서는 전류 불연속 모드(DCM)로 동작하는 연료전지용 900W급 부스트 컨버터에서의 인덕터를 설계하고 이에 따른 인덕터 손실을 분석한다. DCM 구간을 만족하는 인덕턴스 조건에서 인덕터 권선수를 변화시켜 이 때의 손실을 실제 소자의 파라메타를 사용하여 분석한다. 분석한 결과를 이용하여 900W급 부스트 컨버터에서 인덕터의 손실이 최소가 되는 최적 설계점을 도출한다.

• Toxicological Research
• /
• v.5 no.1
• /
• pp.37-41
• /
• 1989

A non-hepatotoxic dose of dichloromethane (DCM) was examined for potential effects on the hepatotoxicity of carbon tetrachloride (CT) in adult male rats. A concomitant treatment of DCM (0.45ml/kg, po) significantly potentiated the hepatotoxicity of CT at varing doses (0.06 to 0.63 ml/kg, po) as determined by increase in SGOT and SGPT activities 24 hn following the treatments. The carboxyhemoglobin (COHb) saturation induced by DCM was significantly decreased by CT treatments. The potentiation of CT hepatotoxicity by DCM does not appear to be associated with increased metabolism of CT.

• Korean Journal of Food Science and Technology
• /
• v.53 no.1
• /
• pp.34-39
• /
• 2021

This study investigated the anti-cancer effects of Salicornia herbacea L. fractions in human ovarian cancer cells (OVCAR-3). S. herbacea powder was extracted with 95% EtOH and sequentially fractionated with hexane, dichloromethane (DCM), ethyl acetate, butanol, and H2O. Further, the growth inhibitory effects of the six fractions were determined using the MTS assay. The DCM fraction dramatically decreased cell viability. Similarly, the cell cycle was arrested at the subG1 phase in DCM-treated cells. To confirm apoptosis, the cells were stained with annexin V/FITC-PI solution. Total, early, and late apoptotic cells were significantly increased in the DCM fraction. The mRNA expression of Bcl-2 was reduced, whereas the pro-apoptotic factors Bax and Bak were increased in DCM fraction-treated cells. These results indicated that the DCM fraction in S. herbacea exhibited strong apoptotic effects through the p53-dependent signaling pathway.