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http://dx.doi.org/10.9721/KJFST.2021.53.1.34

Anti-cancer effects of Salicornia herbacea extract in OVCAR-3 cells by induction of apoptosis pathway  

La, Yu Ri (Department of Smart Foods and Drugs, Inje University)
Lee, You Rim (Department of Smart Foods and Drugs, Inje University)
Lee, Dong Seok (Department of Smart Foods and Drugs, Inje University)
Kim, Soo Hwan (Department of Biomedical Laboratory Science, Gimcheon University)
Lee, Hyeong Seon (Department of Biomedical Laboratory Science, Jungwon University)
Publication Information
Korean Journal of Food Science and Technology / v.53, no.1, 2021 , pp. 34-39 More about this Journal
Abstract
This study investigated the anti-cancer effects of Salicornia herbacea L. fractions in human ovarian cancer cells (OVCAR-3). S. herbacea powder was extracted with 95% EtOH and sequentially fractionated with hexane, dichloromethane (DCM), ethyl acetate, butanol, and H2O. Further, the growth inhibitory effects of the six fractions were determined using the MTS assay. The DCM fraction dramatically decreased cell viability. Similarly, the cell cycle was arrested at the subG1 phase in DCM-treated cells. To confirm apoptosis, the cells were stained with annexin V/FITC-PI solution. Total, early, and late apoptotic cells were significantly increased in the DCM fraction. The mRNA expression of Bcl-2 was reduced, whereas the pro-apoptotic factors Bax and Bak were increased in DCM fraction-treated cells. These results indicated that the DCM fraction in S. herbacea exhibited strong apoptotic effects through the p53-dependent signaling pathway.
Keywords
Salicornia herbacea L.; apoptosis; OVCAR-3 cells; DCM fraction;
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Times Cited By KSCI : 3  (Citation Analysis)
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