• 제목/요약/키워드: DBP

검색결과 442건 처리시간 0.022초

Rapid determination of baculovirus titers an antibody-based assay

  • Kwon, M.S.;Dojimal, T.;Park, Enoch-Y.
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2003년도 생물공학의 동향(XII)
    • /
    • pp.315-319
    • /
    • 2003
  • A novel method is developed to yield virus titers in 10 h, is easy to .perform using 96-well plates, and applicable to both any Autographa californica nucleopolyhyderovirus (AcNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV)-based recombinant baculovirus. This assay uses an antibody to a DNA-binding protein to detect the infected cells via immune-staining. The titer is determined by counting foci produced due to infection of virus under a fluorescent microscopy. The required incubation period was shortened considerably because infected cells expressed viral antigens at the post infection time of 4 h. Therefore, 10 hours were enough to estimate the virus titer including virus infection time, insect cell culture, and estimation of virus titer.

  • PDF

A Study on the Characteristics of Natural Organic Matter and Disinfection By-Product Formation in the Juam Reservoir

  • Shin, Dae-Yewn;Moon, Ok-Ran;Yoon, Mi-Ran;Kim, Nam-Joung;Kang, Gang-Unn;Seo, Gwang-Yeob
    • 한국환경보건학회:학술대회논문집
    • /
    • 한국환경보건학회 2005년도 국제학술대회
    • /
    • pp.259-262
    • /
    • 2005
  • This study aims to identify the relationship between characteristics of aqueous organic matter and chlorination by-products formation potential according to temporal effect of Juam reservoir in Sun-Choen. The molecular weight distribution and chemical composition of precursors and their relationship with disinfection by-products(DBPs) were investigated. Most of the organic matters was responsible for the major DBP precursors in the raw water are small compounds with a molecular weight less than IKDa, Aromatic contents determined by SUVA correlated well with DBPs, THMs, and HAAs formation. Especially, THMFP/DOC showed better correlation with SUVA than HAAFP/DOC and DBPFP/DOC with SUVA in Juam reservoir. Therefore, effective removal of small molecules or hydrophobic organic matter prior to disinfection process will significantly reduce the DBP concentration in the finished water.

  • PDF

Phthalate의 피부자극시험 및 안점막자극시험에 관한 연구 (Dermal and Ocular Irritation Studies of Some Phthalates in Rabbits)

  • 이종권;김주환;이은희;김용규;홍진태;박기숙;안광수;정수연;이선희
    • Toxicological Research
    • /
    • 제17권2호
    • /
    • pp.91-96
    • /
    • 2001
  • Phthalates are widely used as plasticizers to impart softness and flexibility to normally rigid polyvinylchloride products. However, there are not much studies jar dermal and ocular irritation toxicity of phthalates. So we investigated the skin or eye irritation effect of some phthalates which was not reported. The primary skin irritation of diethyl phthalate (DEP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), dipropyl phthalate (DPP) and dipropyl phthalate (DPrP) was studied. The ocular irritation of dibutyl phthalate(DBP), DIDP, DINP, DPP and DPrP was also studied. DEP, DIDP, DINP, DPP, and DPrP were found to be non-irritating to the skin of the test animals. DBP, DIDP, DINP and DPP were found to be non-irritating to the eye of the rabbits. DPrP caused the slight irritations to the eye in 1 or 2 days after treatment but irritation of the animals was soon recovered.

  • PDF

자동혈압계의 오실레이션 신호를 이용한 혈압 측정 (Measuring Blood Pressure Using Oscillation Signal from an Automatic Sphygmomanometer)

  • 김동준;김영수
    • 전기학회논문지
    • /
    • 제61권11호
    • /
    • pp.1720-1724
    • /
    • 2012
  • This study describes an oscillometric-based blood pressure measuring algorithm by detecting turning points of oscillation signal from digitally filtered cuff signals of an automatic sphygmomanometer. The blood pressure measuring algorithm uses a characteristic ratios method from the turning points. The accurate values of the systolic/diastolic blood presures(SBP/DBP) are calculated using the peaks in the ranges of characteristic ratios. Performances of the proposed algorithm and four automatic sphygmomanometers are compared with the mercury manometer(manual type sphygmomanometer), regarding the SBP and DBP values of manual sphygmomanometer as the reference values. The performance test showed the proposed algorithm revealed the best results in errors and a statistical analysis. Therefore this algorithm can be usable in any automatic sphygmomanometers.ssure states. This may be compromising results for subject-independent sensibility evaluation using EEG signal.

Toxicogenomic Gene Profiles using KISTCHIP-400 in MCF-7 cells after Exposure to Di(2-ethylhexyl) Phthalate (DEHP) and Dibutyl Phthalate (DBP)

  • Yun, Hye-Jung;Kim, Youn-Jung;Kim, Eun-Young;Kim, Ick-Young;Ryu, Jae-Chun
    • 한국독성학회:학술대회논문집
    • /
    • 한국독성학회 2003년도 추계학술대회
    • /
    • pp.128-128
    • /
    • 2003
  • There are many synthetic chemicals, such as di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP), used in chemical reaction processes in industry. The establishment of toxicity and detection of synthetic chemicals that may pose a genetic hazard in our enviornment is subjects of great concern at present.(omitted)

  • PDF

Determination of Nitrate in Chromium Plating Solution with Nitrate-Selective Electrodes

  • 강유라;이원;허황;차근식;남학현
    • Bulletin of the Korean Chemical Society
    • /
    • 제16권3호
    • /
    • pp.221-226
    • /
    • 1995
  • A method for determining nitrate in post treatment chromium plating solutions with PVC-based nitrate-selective electrodes is described. PVC-based nitrate-selective membranes which contain TDMANO3 ion-exchanger in PVC/NPOE, PVC/DOA or PVC/DBP matrices, and a commercially available Corning electrode (No. 476134) have been compared in respect of their detection limits, response slopes, selectivities at various pHs, and dynamic response to the hydrochromate ion in basic condition. The PVC/DBP/TDMANO3 membrane electrode was chosen as the ISE detector for the determination of nitrate in the presence of hydrochromate interference. The amount of nitrate in real post treatment chromium plating solution could be determined successfully with this electrode in both static and flow-injection measurements when the sample was properly diluted with an alkaline buffer.