• 제목/요약/키워드: DAT1

검색결과 253건 처리시간 0.017초

주의력결핍 과잉운동장애에서 도파민 수송체 유전자 다형성 (Dopamine Transporter Gene Polymorphism in ADHD)

  • 신동원
    • 생물정신의학
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    • 제9권1호
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    • pp.8-14
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    • 2002
  • Patients with attention deficit hyperactivity disorder(ADHD) have symptoms of inattention, hyperactivity, impulsivity. Symptoms of ADHD are responsive to medications such as methylphenidate, dextroamphetamine, pemoline, and bupropion. The functional change of the dopamine transporter is related to the therapeutic effect of these drugs. This can be one reason for the dopamine transporter to be emphasized in the research field of ADHD. ADHD has a genetic tendency. Since dopamine transporter gene(DAT1) knock out mice were reported to be several times more active than normal mice in a novel situation, lights has been shed on DAT1 as a candidate gene for ADHD. Though there have been several studies which reported an association between DAT1 and ADHD, the association between DAT1 and ADHD is not conclusive. Since Vandenbergh reported the DAT1 polymorphism with variable number of tandem repeats(VNTR), and the racial differences in allelic frequencies of the DAT1, wide ethnic variation in the distribution of the DAT1 polymorphism had been confirmed. Wide ethnic variation in the distribution of the DAT1 suggested that there might be ethnic difference in the association between DAT1 and ADHD. Before applying previous findings to Koreans, verification might be needed for Korean patients with ADHD.

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Criteria of Nitrate Concentration in Soil Solution and Leaf Petiole Juice for Fertigation of Cucumber under Greenhouse Cultivation in Gyeonggi region

  • Park, Jung-Soo;Roh, Ahn-Sung;Jang, Jae-Eun;Kang, Chang-Sung;Kim, Hee-Dong
    • 한국토양비료학회지
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    • 제48권4호
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    • pp.295-304
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    • 2015
  • To develop a technique for efficient management of fertility for cucumber in greenhouse, a quick test method to quantify nitrate ($NO_3{^-}$) content in soil solution and leaf petiole juice using a simple instrument that are easy to use for farmers was investigated. N fertilizer (urea) was applied at 0, 50, 100 and 200% levels of the recommended application rate from 30 days after transplanting to harvest by soil fertigation treatments. Stable results were obtained from analysis of nitrate ($NO_3{^-}$) using top $10^{th}$ or $11^{th}$ leaf petioles collected between 10 to 11 am in the morning. Under the semiforcing culture, $NO_3{^-}$ content of leaf petiole juice was highest at 60 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$content of leaf petiole juice was $2,418{\pm}78{\sim}2,668{\pm}118$ at 45 DAT, $3,032{\pm}90{\sim}3,332{\pm}63$ at 60 DAT, $2,709{\pm}50{\sim}3,158{\pm}155$ at 75 DAT, $2,535{\pm}49{\sim}2,907{\pm}83$ at 90 DAT, and $2,242{\pm}48mg\;L^{-1}$ at 105 DAT. In addition, appropriate $NO_3{^-}$ content of soil solution was $167{\pm}9{\sim}212{\pm}15$ at 45 DAT, $83{\pm}10{\sim}112{\pm}12$ at 60 DAT, $49{\pm}3{\sim}92{\pm}6$ at 75 DAT, $71{\pm}9{\sim}103{\pm}9$ at 90 DAT, and $73{\pm}9mg\;L^{-1}$ at 105 DAT. The cucumber yield at 100% N level of fertigation was $7,770kg\;10a^{-1}$ and no difference in yield was found at 200% N level of fertigation. However, there was 12% decrease in yield at 50% N fertigation and, 17% decrease at 0% N fertigation. Under retarding culture, $NO_3{^-}$ concentration of leaf petiole juice was highest at 55 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$ content of leaf petiole juice was $2,464{\pm}102{\sim}2,651{\pm}33$ at 45 DAT, $3,025{\pm}71{\sim}3,314{\pm}84$ at 55 DAT and $2,488{\pm}92mg\;L^{-1}$ at 65 DAT, respectively. Appropriate $NO_3{^-}$ content of soil solution was $111{\pm}10{\sim}155{\pm}14$ at 45 DAT, $93{\pm}7{\sim}147{\pm}14$ at 55 DAT, $67{\pm}4mg\;L^{-1}$at 65 DAT, respectively. The cucumber yield at 50% N fertigation was not different from $1,697kg\;10a^{-1}$ of 100% N fertigation level and even with that of the 200% N fertigation. However, there was 21% decrease in yield at 0% N fertigation.

재식밀도를 달리한 한국 잔디의 생장반응 (Growth Response of Korean Lawngrass (Zoysia Japonica Steud.) Imposed by Different Plant Densities)

  • 심재성
    • 아시안잔디학회지
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    • 제3권1호
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    • pp.5-12
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    • 1989
  • Tiller numbers per Unit area were increased as plant density heightened followed by little difference in tiller number in 110 days after transplanting(ll0DAT), this being caused by the immediate increment of tiller since' 90DAT During the growth period, the greatest number of green leaves per unit area was attained in 90DAT of both 160-density and 200-density plots, and in l00DAT of 40-, 80-, and I 20-density plots. Thus, the period to reach the maximum green leaf number with the high plant density was earlier than with the' low plant density. The increment of plant density increased the DM weight of ahoveground part and, with 40-densitytreatment. DNI weight was increased 6.1 times as much up to 110DAT as up to 7ODAT but 2 to 2.9 times with other ehe'nsity treatments. The' rate of increase in stolon length was greatest at the period between 80 and 90DAT when DM weight of stolon showed a large increment. Meanwhile, the average number of stolon was 1.7 at 70DAT but was increased up to 10 at 110DAT probably due to accelerative appeance of Ist stolon branch since 8.0DAT. DM weight of stolon was found to exceeded that of root after 90DAT Thus it was concluded that, when turfs is estahlished from sprig. it may be desirable for first mowing practice to be made at least within l00 days after planting.

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한국인에서의 도파민 수송체 유전자 다형성(Dopamine Transporter Gene(DAT1) Polymorphism)과 사회공포증과의 연관성에 관한 예비 연구 (Polymorphism of Dopamine Transporter Gene(DAT1) in Korean Social Phobia Patients:Preliminary Study)

  • 오강섭;윤형근;이민수
    • 생물정신의학
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    • 제11권2호
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    • pp.165-172
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    • 2004
  • Objective:Although polymorphism of dopamine transporter gene(DAT1) has been considered to be implicated in the pathogenesis of social phobia, previous investigations have been inconsistent and controversial. The authors investigated the relationship between DAT1 polymorphism and social phobia in Koreans. Methods:DAT1 and alleles of fifty subjects who met DSM-IV criterion of social phobia, and those of age- & sex- matched fifty normal controls in Korea were compared. Additionally, patients were grouped into generalized(33) and nongeneralized(17) types and DAT1 polymorphism was compared with that of age- & sex- matched controls. DAT1 with variable number of tandem repeats(VNTR) were determined by using polymerase chain reaction. To compare the distribution of the DAT1 polymorphism between different groups, Fisher's exact test was used. Results:There were no significant differences in either genotypic(p=0.451) or allelic(p=0.452) distributions between the social phobia patients and the controls. There also were no differences in genotypic distribution between subtypes of social phobia patients and the controls. Conclusion:We couldn't find any association between DAT1 polymorphism and social phobia. Further studies including larger number of samples and diverse clinical variables should be conducted to elucidate the present findings.

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도파민 수송체의 기능적 특성 및 발현에 관한 연구 (Functional Characterization and Regional Expression of Dopamine Transporter)

  • 이상훈;이송득;성기욱;이동섭;이용성;고재경
    • 약학회지
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    • 제39권2호
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    • pp.161-168
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    • 1995
  • Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter (DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin$^{R}$ were determined by measuring the uptake of $[^3H]$-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated $[^3H]$-dopamine in a dose-dependent manner with a $K_{m}$ of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of $[^3H]$-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain.

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진도관리 자동화를 위한 자료수집기술(DAT) 선정 방법 (Data Acquisition Technology (DAT) Selection Algorithm for Automated Progress Measurement and Management)

  • 강승희;정영수
    • 한국건설관리학회논문집
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    • 제13권1호
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    • pp.77-86
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    • 2012
  • 건설 프로젝트의 성과 측정 및 관리에 있어 진도관리는 기본적이며 핵심적인 업무기능이다. 오랜 기간 동안 다양한 진도 관리 기법이 연구 개발되고 있음에도 불구하고, 자료수집을 위한 과도한 업무부담은 체계화된 진도관리의 장애요소로 지적되어 왔다. 최근 자료수집기술(Data Acquisition Technology, DAT)의 발전은 자동화된 진도관리의 기회를 제공하고 하고 있다. 그럼에도 불구하고 지금까지의 DAT 연구는 특정 공종 또는 특정 기술에 집중하고 있어, 건설사업 전체의 진도관리 자동화에 대한 연구는 이루어지지 않았다. 이러한 관점에서, 본 연구는 건설사업 공종별 최적의 DAT를 선정하는 방법론을 제안하였다. DAT 선정방법은 각 공종별 진도측정의 방법, 대상, 범위, 기술 등의 유형을 분류하여, 이를 DAT 특성별로 최적치를 도출하는 알고리즘으로 구성된다. 제안된 방법론의 검증을 위하여 사례 프로젝트를 이용한 기계적 분석을 실시하고 또한 실무자 평가를 수행한 결과, 대부분 공종에서 동일한 결과를 얻었다. 또한 사례현장 평가결과 중의 하나로서, 노무자의 층별 출입정보를 RFID를 활용하여 측정하는 것이 가장 효율적이며 가장 많은 공종에 적용이 가능한 것으로 나타났다.

Analysis of Mutant Isocitrate Dehydrogenase 1 Immunoexpression, Ki-67 and Programmed Death Ligand 1 in Diffuse Astrocytic Tumours : Study of Single Center in Bandung, Indonesia

  • Bolly, Hendrikus Masang Ban;Faried, Ahmad;Hermanto, Yulius;Lubis, Billy Parulian;Tjahjono, Firman Priguna;Hernowo, Bethy Suryawathy;Arifin, Muhammad Zafrullah
    • Journal of Korean Neurosurgical Society
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    • 제64권1호
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    • pp.100-109
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    • 2021
  • Objective : Diffuse astrocytic tumour (DAT) is a diffuse infiltrative astrocytoma tumour accompanied by molecular parameters such as the presence or absence of isocitrate dehydrogenase (IDH) gene mutations. Ki-67 is a marker for DAT proliferation, while programmed death ligand 1 (PD-L1) indicates an immune evasion mechanism. This study aimed to analyze the correlation among mutant IDH1 R132H, Ki-67, and PD-L1 immunoexpression in the DAT. Methods : A cross-sectional study was carried out on 30 paraffin blocks of DAT cases. Paraffin block samples consist of grade II (n=14), grade III (n=8), and grade IV (n=8). In this study, the immunohistochemistry-staining of mutant IDH1 R132H, Ki-67, and PD-L1 were carried out to determine the frequency of DAT with IDH1 mutations. Results : Our study shown the frequency of IDH1 mutations in grade II 50.0% (7/14), grade III 37.5% (3/8), and grade IV 12.5% (1/8). Our study also showed a difference in Ki-67 and PD-L1 expression between each the degree of DAT histopathology (p=0.0001 and p=0.002, respectively). There was an association between both mutant IDH1 R132H, and Ki-67 with PD-L1 expression in DAT (p=0.0087 and p=0.0049, respectively). Conclusion : DAT with the mutant IDH1 is frequently observed in grade II and small number of grade III. The expression of wild type IDH1, Ki-67, and PD-L1 were found to be higher in high grade DAT (grade III and grade IV). There is a correlation between each of mutant IDH1 status and Ki-67 with PD-L1 expression in DAT.

Involvement of adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 in diallyl trisulfide-induced cytotoxicity in hepatocellular carcinoma cells

  • Guan, Feng;Ding, Youming;He, Yikang;Li, Lu;Yang, Xinyu;Wang, Changhua;Hu, Mingbai
    • The Korean Journal of Physiology and Pharmacology
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    • 제26권6호
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    • pp.457-468
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    • 2022
  • It has been demonstrated that APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is involved in the regulation of several growth-related signaling pathways and thus closely associated with the development and progression of some cancers. Diallyl trisulfide (DAT), a garlic-derived bioactive compound, exerts selective cytotoxicity to various human cancer cells through interfering with pro-survival signaling pathways. However, whether and how DAT affects survival of human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we tested the hypothesis of the involvement of APPL1 in DAT-induced cytotoxicity in HCC HepG2 cells. We found that Lys 63 (K63)-linked polyubiquitination of APPL1 was significantly decreased whereas phosphorylation of APPL1 at serine residues remained unchanged in DAT-treated HepG2 cells. Compared with wild-type APPL1, overexpression of APPL1 K63R mutant dramatically increased cell apoptosis and mitigated cell survival, along with a reduction of phosphorylation of STAT3, Akt, and Erk1/2. In addition, DAT administration markedly reduced protein levels of intracellular TNF receptor-associated factor 6 (TRAF6). Genetic inhibition of TRAF6 decreased K63-linked polyubiquitination of APPL1. Moreover, the cytotoxicity impacts of DAT on HepG2 cells were greatly attenuated by overexpression of wild-type APPL1. Taken together, these results suggest that APPL1 polyubiquitination probably mediates the inhibitory effects of DAT on survival of HepG2 cells by modulating STAT3, Akt, and Erk1/2 pathways.

공황장애와 도파민 수송체 유전자 다형성과의 연관성 (Association between Panic Disorder and Dopamine Transporter Gene(DAT1) Polymorphism)

  • 배승민;임세원;오강섭;이민수
    • 생물정신의학
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    • 제14권1호
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    • pp.55-60
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    • 2007
  • Objectives : There have been many association studies of panic disorder. However, studies about the dopaminergic function in panic disorder have been few. This study was aimed to examine the possible association of dopamine transporter gene(DAT1) polymorphism and panic disorder in Korean population. Methods : Ninety-eight patients with panic disorder(43 male(46.9%), mean age $42.13{\pm}10.88$ years) and one hundred and thirteen comparison subjects(67 male(40.7%), mean age $33.14{\pm}8.55$ years) were tested for DAT1 polymorphism. Genotypes of DAT1 with variable number of tandem repeats(VNTR) were determined using polymerase chain reaction. The differences of allelic frequency and genotype frequency distribution between patient and the control group were tested with Fisher-Freeman-Halton test. Results : There was association between DAT1 polymorphism and panic disorder(allele : p<0.03, genotype : p<0.05). The frequency of 10/10 homozygotes of DAT1 was significantly higher in control group(${\chi}^2$=4.452, df=1, p=0.035). Conclusion : These results in our Korean samples suggest that DAT1 polymorphism might be associated with the vulnerability of panic disorder. Possible association of dopaminergic genes and panic disorder should be investigated with future studies using larger and different population.

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객담 결핵균 도말검사가 음성일때 중합효소연쇄반응검사와 진단적 신뢰도에 관한 연구 (How Reliable is Sputum PCR Test in the Diagnosis of Pulmonary Tuberculosis When Sputum Smear is Negative?)

  • 백승훈;이재명;강민종;손지웅;이승준;김동규;이명구;현인규;정기석;이경화;조현찬
    • Tuberculosis and Respiratory Diseases
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    • 제50권2호
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    • pp.222-228
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    • 2001
  • 목 적 : 객담 항산균 도말 양성환자에서 direct amplification test (DAT) 양성이면 폐결핵으로 확진이 가능하다. 그러나 객담 항산균 도말음성이고 DAT는 양성인 경우에는 DAT 결과가 얼마나 신뢰성이 있는지 의문이었다. 따라서 저자들은 폐결핵이 의심되는 환자에서 객담 항산균 도말 음성이고 DAT 양성일 때 폐결핵진단에 있어서 DAT의 유용성올 살펴보고자 하였다. 방 법 : 1998년 6월1일부터 1999년 5월30일 까지 한림대학교 의료원에서 폐결핵이 의심되어 결핵균 항산균 도말, 배양검사 및 DAT를 시행한 909명을 대상으로 하였다. 활동성 폐결핵의 확진은 결핵균 배양 양성이거나 조직학적으로 건락성 육아종이 증명된 경우로 정의하였다. 임상적 폐결핵의 진단 조건은 방사선 소견상 결핵성 폐침윤이 의심되고 폐렴, 폐암 등이 배제된 환자로 결핵균 배양은 음성이나, 항결핵제 3개월 이상 투여 후 증상 및 방사선 소견상 호전이 있는 경우로 정의하였다. DAT는 Mycobacterium tuberculosis complex에 특징적으로 존재 하는 insertion sequence (IS6110)의 특정 부위만을 특이적으로 증폭시킬 수 있는 TB-$CRkit^{TM}$(한국생공, 대전)를 이용하였으며, 특이도를 높이기 위해 nested PCR검사를 시행하였다. 결 과 : 대상 환자 909 명 중 객담 항산균 도말검사는 335명에서 양성(36.6%)이었으며, 객담 결핵균 PCR이 374명에서 양성(41.1%)으로 판명되었다. 객담 항산균 도말 음성이면서 객담 결핵 PCR 양성인 환자는 39명(4.3%)으로 남자 22명, 여자 17명 이었다. 객담 결핵 PCR 양성인 환자 39 명중 32명에서 폐결핵으로 진단되었다. DAT는 확진된 결핵환자만을 대상으로 할 때 61.5%의 양성 예측율을, 확진된 폐결핵 환자와 임상적으로 진단된 결핵환자를 대상으로 할 때 82.1% 양성 예측율을 나타냈었고 위양성율은 18.0%였다. 결 론 : 이상의 결과로 임상적으로 폐결핵이 의심되는 객담 항산균 도말 음성인 환자에서 객담 DAT 양성일 때 폐결핵의 조기 진단에 중요한 수단으로 이용 될 수 있을 것으로 사료되었다.

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