• Korean Journal of Biological Psychiatry
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• v.9 no.1
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• pp.8-14
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• 2002

Patients with attention deficit hyperactivity disorder(ADHD) have symptoms of inattention, hyperactivity, impulsivity. Symptoms of ADHD are responsive to medications such as methylphenidate, dextroamphetamine, pemoline, and bupropion. The functional change of the dopamine transporter is related to the therapeutic effect of these drugs. This can be one reason for the dopamine transporter to be emphasized in the research field of ADHD. ADHD has a genetic tendency. Since dopamine transporter gene(DAT1) knock out mice were reported to be several times more active than normal mice in a novel situation, lights has been shed on DAT1 as a candidate gene for ADHD. Though there have been several studies which reported an association between DAT1 and ADHD, the association between DAT1 and ADHD is not conclusive. Since Vandenbergh reported the DAT1 polymorphism with variable number of tandem repeats(VNTR), and the racial differences in allelic frequencies of the DAT1, wide ethnic variation in the distribution of the DAT1 polymorphism had been confirmed. Wide ethnic variation in the distribution of the DAT1 suggested that there might be ethnic difference in the association between DAT1 and ADHD. Before applying previous findings to Koreans, verification might be needed for Korean patients with ADHD.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.4
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• pp.295-304
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• 2015

To develop a technique for efficient management of fertility for cucumber in greenhouse, a quick test method to quantify nitrate ($NO_3{^-}$) content in soil solution and leaf petiole juice using a simple instrument that are easy to use for farmers was investigated. N fertilizer (urea) was applied at 0, 50, 100 and 200% levels of the recommended application rate from 30 days after transplanting to harvest by soil fertigation treatments. Stable results were obtained from analysis of nitrate ($NO_3{^-}$) using top $10^{th}$ or $11^{th}$ leaf petioles collected between 10 to 11 am in the morning. Under the semiforcing culture, $NO_3{^-}$ content of leaf petiole juice was highest at 60 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$content of leaf petiole juice was $2,418{\pm}78{\sim}2,668{\pm}118$ at 45 DAT, $3,032{\pm}90{\sim}3,332{\pm}63$ at 60 DAT, $2,709{\pm}50{\sim}3,158{\pm}155$ at 75 DAT, $2,535{\pm}49{\sim}2,907{\pm}83$ at 90 DAT, and $2,242{\pm}48mg\;L^{-1}$ at 105 DAT. In addition, appropriate $NO_3{^-}$ content of soil solution was $167{\pm}9{\sim}212{\pm}15$ at 45 DAT, $83{\pm}10{\sim}112{\pm}12$ at 60 DAT, $49{\pm}3{\sim}92{\pm}6$ at 75 DAT, $71{\pm}9{\sim}103{\pm}9$ at 90 DAT, and $73{\pm}9mg\;L^{-1}$ at 105 DAT. The cucumber yield at 100% N level of fertigation was $7,770kg\;10a^{-1}$ and no difference in yield was found at 200% N level of fertigation. However, there was 12% decrease in yield at 50% N fertigation and, 17% decrease at 0% N fertigation. Under retarding culture, $NO_3{^-}$ concentration of leaf petiole juice was highest at 55 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$ content of leaf petiole juice was $2,464{\pm}102{\sim}2,651{\pm}33$ at 45 DAT, $3,025{\pm}71{\sim}3,314{\pm}84$ at 55 DAT and $2,488{\pm}92mg\;L^{-1}$ at 65 DAT, respectively. Appropriate $NO_3{^-}$ content of soil solution was $111{\pm}10{\sim}155{\pm}14$ at 45 DAT, $93{\pm}7{\sim}147{\pm}14$ at 55 DAT, $67{\pm}4mg\;L^{-1}$at 65 DAT, respectively. The cucumber yield at 50% N fertigation was not different from $1,697kg\;10a^{-1}$ of 100% N fertigation level and even with that of the 200% N fertigation. However, there was 21% decrease in yield at 0% N fertigation.

• Asian Journal of Turfgrass Science
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• v.3 no.1
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• pp.5-12
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• 1989

Tiller numbers per Unit area were increased as plant density heightened followed by little difference in tiller number in 110 days after transplanting(ll0DAT), this being caused by the immediate increment of tiller since' 90DAT During the growth period, the greatest number of green leaves per unit area was attained in 90DAT of both 160-density and 200-density plots, and in l00DAT of 40-, 80-, and I 20-density plots. Thus, the period to reach the maximum green leaf number with the high plant density was earlier than with the' low plant density. The increment of plant density increased the DM weight of ahoveground part and, with 40-densitytreatment. DNI weight was increased 6.1 times as much up to 110DAT as up to 7ODAT but 2 to 2.9 times with other ehe'nsity treatments. The' rate of increase in stolon length was greatest at the period between 80 and 90DAT when DM weight of stolon showed a large increment. Meanwhile, the average number of stolon was 1.7 at 70DAT but was increased up to 10 at 110DAT probably due to accelerative appeance of Ist stolon branch since 8.0DAT. DM weight of stolon was found to exceeded that of root after 90DAT Thus it was concluded that, when turfs is estahlished from sprig. it may be desirable for first mowing practice to be made at least within l00 days after planting.

• Korean Journal of Biological Psychiatry
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• v.11 no.2
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• pp.165-172
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• 2004

Objective:Although polymorphism of dopamine transporter gene(DAT1) has been considered to be implicated in the pathogenesis of social phobia, previous investigations have been inconsistent and controversial. The authors investigated the relationship between DAT1 polymorphism and social phobia in Koreans. Methods:DAT1 and alleles of fifty subjects who met DSM-IV criterion of social phobia, and those of age- & sex- matched fifty normal controls in Korea were compared. Additionally, patients were grouped into generalized(33) and nongeneralized(17) types and DAT1 polymorphism was compared with that of age- & sex- matched controls. DAT1 with variable number of tandem repeats(VNTR) were determined by using polymerase chain reaction. To compare the distribution of the DAT1 polymorphism between different groups, Fisher's exact test was used. Results:There were no significant differences in either genotypic(p=0.451) or allelic(p=0.452) distributions between the social phobia patients and the controls. There also were no differences in genotypic distribution between subtypes of social phobia patients and the controls. Conclusion:We couldn't find any association between DAT1 polymorphism and social phobia. Further studies including larger number of samples and diverse clinical variables should be conducted to elucidate the present findings.

• YAKHAK HOEJI
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• v.39 no.2
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• pp.161-168
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• 1995

Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter (DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin$^{R}$ were determined by measuring the uptake of $[^3H]$-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated $[^3H]$-dopamine in a dose-dependent manner with a $K_{m}$ of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of $[^3H]$-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain.

• Korean Journal of Construction Engineering and Management
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• v.13 no.1
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• pp.77-86
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• 2012

Progress measurement provides project participants with valuable information in terms of 'current status', 'corrective countermeasure', and 'forecast of future risks'. However, the managerial effort (or workload) required to acquire and maintain detailed progress data has been the major barrier to practical implementation. Even though recent efforts in utilizing data acquisition technologies (DATs) have actively attempted to automate the progress measurement process, previous DAT research has only been focused on limited construction tasks or selected technologies. This paper proposes a DAT selection algorithm that automatically assign an appropriate DAT for every commodity item for an entire constriction project. Five different criteria were identified and developed in order to comprehend specific requirements for automating the progress measurement of each commodity group. A case-study and also a survey from practitioners were conducted in order to validate the proposed methodology.

• Journal of Korean Neurosurgical Society
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• v.64 no.1
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• pp.100-109
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• 2021

Objective : Diffuse astrocytic tumour (DAT) is a diffuse infiltrative astrocytoma tumour accompanied by molecular parameters such as the presence or absence of isocitrate dehydrogenase (IDH) gene mutations. Ki-67 is a marker for DAT proliferation, while programmed death ligand 1 (PD-L1) indicates an immune evasion mechanism. This study aimed to analyze the correlation among mutant IDH1 R132H, Ki-67, and PD-L1 immunoexpression in the DAT. Methods : A cross-sectional study was carried out on 30 paraffin blocks of DAT cases. Paraffin block samples consist of grade II (n=14), grade III (n=8), and grade IV (n=8). In this study, the immunohistochemistry-staining of mutant IDH1 R132H, Ki-67, and PD-L1 were carried out to determine the frequency of DAT with IDH1 mutations. Results : Our study shown the frequency of IDH1 mutations in grade II 50.0% (7/14), grade III 37.5% (3/8), and grade IV 12.5% (1/8). Our study also showed a difference in Ki-67 and PD-L1 expression between each the degree of DAT histopathology (p=0.0001 and p=0.002, respectively). There was an association between both mutant IDH1 R132H, and Ki-67 with PD-L1 expression in DAT (p=0.0087 and p=0.0049, respectively). Conclusion : DAT with the mutant IDH1 is frequently observed in grade II and small number of grade III. The expression of wild type IDH1, Ki-67, and PD-L1 were found to be higher in high grade DAT (grade III and grade IV). There is a correlation between each of mutant IDH1 status and Ki-67 with PD-L1 expression in DAT.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.457-468
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• 2022

It has been demonstrated that APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is involved in the regulation of several growth-related signaling pathways and thus closely associated with the development and progression of some cancers. Diallyl trisulfide (DAT), a garlic-derived bioactive compound, exerts selective cytotoxicity to various human cancer cells through interfering with pro-survival signaling pathways. However, whether and how DAT affects survival of human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we tested the hypothesis of the involvement of APPL1 in DAT-induced cytotoxicity in HCC HepG2 cells. We found that Lys 63 (K63)-linked polyubiquitination of APPL1 was significantly decreased whereas phosphorylation of APPL1 at serine residues remained unchanged in DAT-treated HepG2 cells. Compared with wild-type APPL1, overexpression of APPL1 K63R mutant dramatically increased cell apoptosis and mitigated cell survival, along with a reduction of phosphorylation of STAT3, Akt, and Erk1/2. In addition, DAT administration markedly reduced protein levels of intracellular TNF receptor-associated factor 6 (TRAF6). Genetic inhibition of TRAF6 decreased K63-linked polyubiquitination of APPL1. Moreover, the cytotoxicity impacts of DAT on HepG2 cells were greatly attenuated by overexpression of wild-type APPL1. Taken together, these results suggest that APPL1 polyubiquitination probably mediates the inhibitory effects of DAT on survival of HepG2 cells by modulating STAT3, Akt, and Erk1/2 pathways.

• Korean Journal of Biological Psychiatry
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• v.14 no.1
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• pp.55-60
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• 2007

Objectives : There have been many association studies of panic disorder. However, studies about the dopaminergic function in panic disorder have been few. This study was aimed to examine the possible association of dopamine transporter gene(DAT1) polymorphism and panic disorder in Korean population. Methods : Ninety-eight patients with panic disorder(43 male(46.9%), mean age $42.13{\pm}10.88$ years) and one hundred and thirteen comparison subjects(67 male(40.7%), mean age $33.14{\pm}8.55$ years) were tested for DAT1 polymorphism. Genotypes of DAT1 with variable number of tandem repeats(VNTR) were determined using polymerase chain reaction. The differences of allelic frequency and genotype frequency distribution between patient and the control group were tested with Fisher-Freeman-Halton test. Results : There was association between DAT1 polymorphism and panic disorder(allele : p<0.03, genotype : p<0.05). The frequency of 10/10 homozygotes of DAT1 was significantly higher in control group(${\chi}^2$=4.452, df=1, p=0.035). Conclusion : These results in our Korean samples suggest that DAT1 polymorphism might be associated with the vulnerability of panic disorder. Possible association of dopaminergic genes and panic disorder should be investigated with future studies using larger and different population.

• Tuberculosis and Respiratory Diseases
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• v.50 no.2
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• pp.222-228
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• 2001

Backgrounds : Recent technological developments have introduced a new method to identifying M. tuberculosis complex DNA in clinical samples directly. The direct amplification test (DAT) is approved for identifying M. tuberculosis complex in respiratory specimens that are smear-positive for acid-fast bacilli (AFB). When there is a discrepancy between the AFB smear and DAT, no information on their clinical utility is currently available. In this study, the diagnostic reliability of DAT was investigated in suspected pulmonary tuberculosis patients whose sputum AFB smear was negative. Methods : From June 1, 1998 through May 30, 1999, 909 patients with presumed active pulmonary tuberculosis were enrolled. A sputum AFB stain, culture, DAT and/or biopsy were performed. Using the criteria of clinical tuberculosis or confirmed tuberculosis, the positive predictive value of DAT in diagnosing pulmonary tuberculosis was investigated. Results : The positive predictive value of DAT was 82.1% by the clinically active tuberculosis criteria. However, it decreased to 61.5% when diagnosis was restricted to only to culture positive or biopsy proven cases. The false positive rate of DAT was 18.0%. Conclusion : The DAT is a valuable diagnostic method in suspected patients whose sputum AFB is was negative.