• Title/Summary/Keyword: DAP

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Isolation of Glucose Isomerase-Producing Microorganism, Streptomyces luteogriseus and Determination of Fermentation Conditions (포도당 이성화 효소 생산성 신균주 Streptomyces luteogriseus의 분리 및 발효 특성)

  • 홍승서;백진기;이현수;국승욱;박관화
    • Microbiology and Biotechnology Letters
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    • v.19 no.3
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    • pp.296-302
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    • 1991
  • Glucose isomerase producer, which produces 488 U/ml of glucose isomerase activity in 500 ml flask scale, was isolated among 666 isolates of Actinomycetes from pine forest soil samples. The isolate was identified as Streptomyces luteogriseus through the studies about morphology (spiral aerial mycelia), cell wall type (Type I), spore chains (spiral form), pigment formation (gray melanine pigment) & metabolism (sugar utilization etc). The optimum conditions of fermentation were determined in 500 ml flask scale. The enzyme production was reached maximum after 4 days at pH 6.0~8.0 and 27~$30^{\circ}C$ in the medium containing 1.5~3.0% of xylose; 0.5-0.8% of glucose; 0.1% of $MgSO_4.7H_20$; 0.05% of $CoCI_2-6H_20$; 7.5% of corn steep liquor.

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Identification of Streptomyces sp. KH29, Which Produces an Antibiotic Substance Processing an Inhibitory Activity Against Multidrug-Resistant Acinetobacter baumannii

  • Lee, Keyong-Ho;Kim, Gye-Woong;Rhee, Ki-Hyeong
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1672-1676
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    • 2010
  • The Actinomycete strain KH29 is antagonistic to the multidrug-resistant Acinetobacter baumannii. Based on the diaminopimelic acid (DAP) type, and the morphological and physiological characteristics observed through the use of scanning electron microscopy (SEM), KH29 was confirmed as belonging to the genus Streptomyces. By way of its noted 16S rDNA nucleotide sequences, KH29 was found to have a relationship with Streptomyces cinnamonensis. The production of an antibiotic from this strain was found to be most favorable when cultured with glucose, polypeptone, and yeast extract (PY) medium for 6 days at $27^{\circ}C$. The antibiotic produced was identified, through comparisons with reported spectral data including MS and NMR as a cyclo(L-tryptophanyl-L-tryptophanyl). Cyclo(L-Trp-L-Trp), from the PY cultures of KH29, was seen to be highly effective against 41 of 49 multidrug-resistant Acinetobacter baumannii. Furthermore, cyclo(L-Trp-L-Trp) had antimicrobial activity against Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Saccharomyces cerevisiae, Aspergillus niger, and Candida albicans, However, it was ineffective against Streptomyces murinus.

Study of Cytotoxicity of an Actinomycete Isolated in Korea (토양에서 분리한 방선균의 세포 독성에 관한 연구)

  • Park, Joon-Koo;Choi, Boung-Don;Kim, Seung-Chul;Ryeom, Kon
    • Environmental Analysis Health and Toxicology
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    • v.8 no.3_4
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    • pp.7-12
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    • 1993
  • An Actinomycete strain isolated from Mt. Dea-Dun had a strong antifungal activity. The culture brith produced by isolated strain showed only antifungal activity against fungi with the exception of yeast and bacteria. It was heat stable, dissolved in ehtylacetate. The concentrated antifungal agent showed cytotoxicity against HEP-2 and HeLa as tumor cell line, and showed weak cytotoxicity against VERO 36 as normal cell line. Morphological and physiological characteristics were tested with isolated strain. The spore color of isolated strain was gray. It had a short chain and produced brown colored lytic substance in yeast extract-malt agar. The cell wall of isolated strain was composed of meso-DAP, and we suggested it as genus Actinomadura. In the existing of chemical inhibitor, isolated strain grew on the condition of 0.0001% crystal violet, 0.1% phenol, 0.01% sodium azide and 10% sodium chloride. Carbon utilization of isolated strain was shown that glucose, sucrose, manitol and sodium citrate were well utilized.

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Morphological Variation of Bainitic Ferrite in Transformation Process of Austempered Ductile Iron (구상흑연주철의 Bainite변태과정에서 Bainitic Ferrite의 형상변화)

  • Choi, Dap-Chun
    • Journal of Korea Foundry Society
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    • v.12 no.5
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    • pp.403-411
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    • 1992
  • The growth characteristics of bainite at early stage in the fast quenched spheroidal graphite cast irons containing 0.06%Mn and 0.45%Mn during austempering process, was investigated with optical and scanning electron microscope. The following results regarding the effects of Mn and isothermal heat treatment on the morphological variation of bainitic ferrite were obtained. The morphology of bainite varies from acicular below 350$^{\circ}C$ to feather shape above 350$^{\circ}C$. The period of isothermal treatment also affects the shape of bainite at the fixed temperature. At 350$^{\circ}C$, bainite is bamboo leaf-like up to 200 secs of isothermal holding time and with further increasing time up to 300 secs, changes to a mixed structure consisting of both feather and bamboo leaf and, finally becomes all feather shape at 900 secs. The morphology of bainitic ferrite formed at early stage of 300$^{\circ}C$ isothermal treatment is similar to that of bainitic ferrite formed at 250$^{\circ}C$ or 350$^{\circ}C$ with unbranched, linear ferrite. However, bainitic ferrite divides into branches with increasing isothermal treatment, which occurs more fast at 400$^{\circ}C$ than at 350$^{\circ}C$. The difference in adding amount of Mn influences the morphology of bainitic ferrite in upper bainite. The bainitic ferrite with 0.45%Mn is observed to be more stable than that with 0.06%Mn, remaining unbranched for a longer period at the same temperature.

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Realization and Analysis of p-Type ZnO:Al Thin Film by RF Magnetron Sputtering

  • Jin, Hu-Jie;Jeong, Yun-Hwan;Park, Choon-Bae
    • Transactions on Electrical and Electronic Materials
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    • v.9 no.2
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    • pp.67-72
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    • 2008
  • Al-doped p-type ZnO thin films were fabricated by RF magnetron sputtering on n-Si (100) and homo-buffer layers in pure oxygen ambient. ZnO ceramic mixed with 2 wt% $Al_2O_3$ was selected as a sputtering target. XRD spectra show that the Al-doped ZnO thin films have ZnO crystal structure. Hall Effect experiments with Van der Pauw configuration show that p-type carrier concentrations are arranged from $1.66{\times}10^{16}$ to $4.04{\times}10^{18}\;cm^{-2}$, mobilities from 0.194 to $198\;cm^2V{-1}s^{-1}$ and resistivities from 0.0963 to $18.4\;{\Omega}cm$. FESEM cross section images of different parts of a p-type ZnO:Al thin film annealed at $800^{\circ}C$ show a compact structure. Measurement for same sample shows that density is $5.40\;cm^{-3}$ which is smaller than theoretically calculated value of $5.67\;cm^{-3}$. Photoluminescence (PL) spectra at 10 K show a shoulder peak of p-type ZnO film at about 3.117 eV which is ascribed to electron transition from donor level to acceptor level (DAP).

A Study of Gwa-du (裹肚) in the Joseon Dynasty (조선시대 과두(裹肚)에 관한 연구)

  • Song, Mi-Kyung
    • Journal of the Korean Society of Costume
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    • v.60 no.1
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    • pp.76-90
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    • 2010
  • Gwa-du(裹肚), name of a garment, appears often from documentary records of Joseon Dynasty. It is assumed as one of the upper garments for men, and according to the record, it was worn as a set with Dan-ryung(團領), Dap-ho(搭胡), Chul-rik(帖裏), Han-sam(汗衫), pants and socks. After the Japanese Invasion of Korea in the 17th century, it changes to a shape of wrapping clothes with straps at four corners, and used to cover the stomach of a corpse like sash. From 16th-17th century, before the Japanese Invasion of Korea, the excavated costume shows a clothing which takes a role of Jegori, longer than Han-sam(汗衫) and shorter than Po(袍). In this study, this clothing is called Gwa-du(裹肚). This study examined the usage and shape of Kwa-du from some documentary records- "朝鮮王朝實錄-The Annals of the Joseon Dynasty", "禮書- Book of Manners", "宮中件記- Googjoonghalkee, recording court goods and commodities lists" and more with actual clothing. Kwa-du is presumed to have changed to men's Jegori So-chang-ui(小氅衣) after 17th century.

Evaluation on Flexural Behavior of Double-tee Slabs with the Least Depth from Optimization Process (최적이론에 의하여 설계된 최소 깊이 더블티 슬래브의 휨거동 평가)

  • 유승룡;김대훈;유재천
    • Journal of the Korea Concrete Institute
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    • v.11 no.3
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    • pp.141-152
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    • 1999
  • Precast prestressed double-tee slab may be designed by the PCI Design Handbook. It is based on the bridge construction and is required for reorganization for the use of buildings in the domestic construction environments. Much enhanced sections are developed from the reforming process on the determined design factors in the previous experimental works on double tees. Pre-determined shape, reinforcement detail, and 5000 psi concrete strength can not be expected as the best solution for the domestic construction requirements because large amount of use on that systems are anticipated. Flexural tests are performed on four full-scale 12.5m proto-type models, "least depth double tee", which are resulted from the optimization process. Domestic superimposed live load regulation, domestic material properties which is available to product, building design requirements and economy in construction are considered as the main factors to establish. the first two sections are double tee section for 1.2 ton/$\m^2$ market live load with straight and one-point depressed strands and the second two are for 0.6 ton/$\m^2$ parking live load with those strand types. All of the specimens tested fully comply with the flexural strength requirements as specified by ACI 318-95. However, the research has shown that following improved considerations are needed for better result in practice. The locations and method of connection for the lowest bottom mild bar, connection method between precast and cast-in-place concrete, and dap-end reinforcement are need to be improved.

Application of 3GPP LTE and IEEE 802.11p Systems to Ship Ad-Hoc Network with the Existence of ISI

  • Su, Xin;Hui, Bing;Chang, KyungHi;Jin, Gwangja
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37A no.12
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    • pp.1106-1114
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    • 2012
  • In order to provide high data rate and real time services under maritime environment, link-level performance of ship ad-hoc network (SANET) based on 3GPP LTE and IEEE 802.11p (WAVE) specifications are investigated and discussed in this paper. The measured maritime channel, whose delay spread is longer than the length of guard interval (GI) of both 3GPP LTE and IEEE 802.11p specifications, is adopted for the link-level simulations. For the purpose of eliminating inter-symbol interference (ISI) due to insufficient GI length, double antenna pattern (DAP) scheme and advanced time-domain decision-feedback equalizer (DFE) are proposed for LTE and WAVE systems, respectively. The proposed DFE removes the ISI in a same manner as the residual inter-symbol interference cancellation (RISIC) algorithm, but the inter-carrier interference (ICI) is reduced via cyclicity removal instead of cyclicity restoration used in the RISIC algorithm. Compared with existing schemes, our proposed DFE is a robust technique to overcome the severe ISI channel which has a comparatively large delay spread. Based on simulation results, not only comparisons between systems are discussed, but also some reformative suggestions are given.

The Effects of Gas Bubbles on the Graphite Spheroidization in Cast Iron (주철의 흑연구상화에 미치는 기포의 영향)

  • Park, Shil-La;Choi, Dap-Chun;Kim, Kwan-Hyu
    • Journal of Korea Foundry Society
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    • v.9 no.6
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    • pp.474-482
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    • 1989
  • The aim of this research is to investigate the effects of gas bubbles on the formation of spheroidal graphite in cast iron, Fe-Si-8%Mg alloy, mischmetal hydride($MmH_2$) and $CaCO_3$, which discharge various amounts of Mg, $H_2$ and $Co_2$, gases, were added to Fe-3.9% C-2.0%Si melt and the melt was innoculated with 0.3wt% of 75%Fe-Si. The spheroidal graphites and/or compacted vermicular graphites were produced with more than 0.625cc/g of Mg gas or more than 0.3125cc/g of $H_2$ gas while $CO_2$ gas did not contribute to graphite spheroidization. Nodule counts increased with the amount of Fe-Si-Mg added ; but they decreased with the amount of $MmH_2$ added because the number of effective gas bubbles decrease with the increase in Mm residual. The bull's eye structure was revealed with 0.625cc/g, 1.25cc/g of Mg and 0.3125cc/g of $H_2$ ; the ledeburite structure was revealed with more than 0.625cc/g of $H_2$.

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