• Title/Summary/Keyword: DAD

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Composition and Quantitative Analysis of Stilbenoids in Mulberry (Morus alba L.) Leaves and Fruits with DAD/UV HPLC (DAD/UV HPLC를 이용한 뽕잎과 오디(Morus alba L.)에 함유된 Stilbenoids 조성 및 함량 분석)

  • Kim, Ji-Sun;Ha, Tae-Youl;Ahn, Ji-Yun;Kim, Hyun-Ku;Kim, Sun-A
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.1
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    • pp.124-128
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    • 2008
  • This study was aimed to analyze the composition and to quantify the contents of stilbenoids in the leaves and fruits of Morus alba L. using high performance liquid chromatography with phodtodiode array detector and UV detector. Optimal wavelength for the detection of various stilbenoids such as resveratrol, piceatannol, rhapontigenin, astringin, pterostilbene, piceid, rhaponticin and vitisin A was screened by DAD detector and set to 308 nm. Seven kinds of stilbenoids except vitisin A were identified in fruits, while 5 kinds of stilbenoids in leaves. Total stilbenoids contents were $609.15{\pm}7.24$ mg/100 g d.w. in fruits and $188.57{\pm}1.70$ mg/100 g d.w in leaves. Stilbenoids contents in fruits were 3 times higher than those in leaves. Rhaponticin was the most profound stilbene, analyzed to $389.26{\pm}5.22$ mg/100 g d.w. (63.8% of total stilbenoids) in fruits and $99.17{\pm}2.79$ mg/100 g d.w. (52.5% of total stilbenoids) in leaves. Astringin and piceatannol were only detected in fruits and vitisin A was not detected. Contents of piceid and rhaponticin were higher than those of aglycone forms, rhapontigenin and resveratrol.

Stability Test and Analysis of Rubia cordifolia for Carcinogenicity Study (천초근의 발암성 연구를 위한 분석 및 안정성 시험)

  • Kim, Dae-Hyun;Park, Jin-Ho;Kim, Seung-Hyun;Sung, Sang-Hyun
    • Korean Journal of Pharmacognosy
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    • v.43 no.2
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    • pp.147-151
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    • 2012
  • The marker constituent was isolated from Rubia cordifolia as a major compound. Quantitative method for the marker of the natural medicine was developed using HPLC-DAD and using established method the extract of Rubia cordifolia was evaluated. In addition, long term and accelerated stability test in the extract was examined for six months. No significant change in content of the marker constituent of the extract observed during the period of long term test.

Similarity Analysis of Chemical Profiles of Scrophularia buergeriana Based on HPLC-DAD

  • Kim, Dae-Hyun;Park, Jin-Ho;Kim, Young-Choong;Sung, Sang-Hyun;Kim, Seung-Hyun
    • Natural Product Sciences
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    • v.17 no.3
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    • pp.225-229
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    • 2011
  • A new approach for similarity analysis of chemical profiles of Scrophularia buergeriana was developed. The roots of S. buergeriana collected in Andong, Korea were analyzed using HPLC-DAD and the peak areas of the chromatograms were used to construct the chemical profiles of S. buergeriana. The analytical conditions were validated for specificity, precision and repeatability. The standard chemical profiles were established from the chromatograms of nine standard analytical extracts from three batches. The correlation coefficients of peak areas of characteristic compounds between the chromatograms of the standard and test samples were calculated for quantitative expression of the chemical profiles. The results showed that the HPLC chemical profiles of the samples of same geographic origin were relatively consistent. This new approach could be applied to the quality assessment of herbal medicines with complex chemical compositions.

The Change of PMP According to Recent Meteorological Variation (최근의 기상변동과 PMP의 변화)

  • Kim, Nam Won;Won, Yoo Seung
    • Proceedings of the Korea Water Resources Association Conference
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    • 2004.05b
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    • pp.1203-1207
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    • 2004
  • "1999년도 수자원관리기법개발연구조사보고서"(건설교통부, 2000b)에서는 1969년부터 1999년까지 우리나라에서 발생한 132개의 주요호우를 분석하여 전국적으로 일반화된 PMP를 추정(건설교통부, 2000a)하였다. 그러나 1999년 이후, 2002년 태풍 루사(Rusa) 등의 극치호우가 발생하였으며, 이로 인해 기 추정된 PMP에 영향을 주었을 가능성이 크다. 따라서 본 인구에서는 2000년부터 2003년까지 발생한 주요 호우의 DAD 관계를 분석하였고, 이들 호우에 내한 크기와 규모를 정량적으로 평가하였으며, 이를 이용하여 2000년 이후 발생한 호우가 PMP에 어느 정도 또는 어떠한 형태로 영향을 줄 것인지를 분석하였다.

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Estimation of Probable Maximum Depth-Area-Duration by Moisture Maximization over the Geumgang River Basin (금강유역에 내린 호우의 수분최대화에 의한 가능 최대 DAD의 산정)

  • Lee, Kwang-Ho
    • Atmosphere
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    • v.16 no.2
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    • pp.55-65
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    • 2006
  • The characteristics of Depth-Area-Duration (DAD) for 50 storms over the Geumgang river basin have been analysed in terms of various storm causes using the precipitation data during the period from 1984 to 2003. Results show that the ratio of the precipitation depth to duration, and the ratio of decrease in the precipitation depth to area are the largest in the case of the tropical cyclone. Storm maximization ratios are in the range 1.03 to 2.66 for the 50 selected heavy precipitation cases over Geumgang river basin, with the largest value for the tropical cyclone case, suggesting that the tropical cyclone could cause heavier precipitation than the other storms. In addition, the 24-hour probable maximum precipitation for the Geumgang river basin is estimated to be about 745 mm in the maximum precipitation area.

Comparison of deck-anchored damper and clipped tuned mass damper on cable vibration reduction

  • Wu, W.J.;Cai, C.S.
    • Structural Engineering and Mechanics
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    • v.32 no.6
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    • pp.741-754
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    • 2009
  • Excessive cable vibrations are detrimental to cable-stayed bridges. Increasing the system damping of cables is a key solution to resolve this severe problem. Equations representing the dynamic characteristics of an inclined cable with a Deck-Anchored Damper (DAD) or with a Clipped Tuned Mass Dampers (CTMD) are reviewed. A theoretical comparison on the performance of cable vibration reduction between the cable-DAD system and the cable-CTMD systems is thoroughly discussed. Optimal system modal damping for the free vibration and transfer functions for the forced vibration for the two cabledamper systems are addressed and compared in detail. Design examples for these two different dampers are also provided.

Oxidation of Alkane Derivatives by Corynebacterium sp. (Alkane 유도체의 미생물학적 산화 제1보)

  • 이종근;이상준
    • Korean Journal of Microbiology
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    • v.21 no.4
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    • pp.185-190
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    • 1983
  • Twelve Microorganisms capable of utilizing diaminododecane were isolated from the soil by enrichment culture technique. Seven strains of these were identified as Corynebacterium. The isolated strains were tested for the ability to utilize as carbon source, 10 different kind of alkane derivatives containing CN, $NH_2$, Cl, and SH groups. Laurylcyanide, dicyanooetane, chlorodecane, and dichlorodecane were not utilized by any of the isolated strains; putrescine dihydrochloride, cadaverine dihydrochloride, diaminododecane, and n-dodecane were utilized by all of the isolated strains; and all of the isolated strains except DAD 2-3 could utilize dodecylmercaptan. The alkane derivatives that did not serve as ,growth substrates were tested further in oxidation tests using resting cell preparation. Alkane derivatives that are being oxidized by all of the isolated strains are laurylcyanide and dichlorodecane. Dicyanooctane was also oxidized by all of the isolated strains except DAD 30L, chlorodecane was the only oxidized by the three isolated strains. The most remarkable substrate that is being oxidized is dichlorodecane containing CN groups diterminally. Evidence obtained with thin layer chromatography of ,ethyl acetate extracts of culture broth of isolated strains grown in some alkane derivatives shows that these alkane derivatives are degraded.

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