• Title/Summary/Keyword: D1S80

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Fundamental Investigation of Non-invasive Determination of Glucose by Near Infrared Spectrophotometry (근적외선 분광법을 이용한 비침투적 혈당 분석법 개발에 관한 기초 연구)

  • Kim, Hyo J.;Woo, Young A.;Chang, Soo H.;Cho, Chang H.;Cantrell, Kevin;Piepmeier, Edward H.
    • Analytical Science and Technology
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    • v.11 no.1
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    • pp.47-53
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    • 1998
  • This study is to improve the diagnosis of diabetes mellitus and the self-monitoring of blood glucose in people with diabetes by providing a non-invasive method of monitoring blood glucose. A near-infrared (NIR) spectrophotometer was used to measure absorption spectra of 80 glucose samples ranges from 1 mg/dL to 200 mg/dL, and shows the standard error of prediction 1.8 mg/dL. Also, to investigate the effect of interference in blood, NaCl and sand were added in glucose and found the standard error of prediction of 2.8 mg/dL and 3.8 mg/dL, respectively. A new and more accurate calibration system for the spectrophotometer was developed from systematic study of light scattering, which cause nonlinear spectrophotometer response.

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Correlation of Expression of p53, Cylcin D1 and Galectin-3 in Papillary Carcinoma and Follicular Carcinoma

  • Back, Oun-Cheol
    • Korean Journal of Clinical Laboratory Science
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    • v.45 no.1
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    • pp.32-36
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    • 2013
  • The thyroid is the organ that has the greatest risk of malignant tumors among the endocrine tumors. The papillary carcinoma occupies 80% of the entire thyroid tumors. Immunohistochemical staining of galectin-3 has usually been used in differentiating papillary carcinoma and follicular carcinoma. The p53 gene of the cell cycle is a tumor suppressor gene acting in on the control points. The cyclin D1 genes in the cell cycle, involved in the implementation of G1 and S phase, plays an important role in the progression of thyroid tumors. This research compares and analyzes correlation between papillary carcinoma, follicular carcinoma, p53, cyclin D1 and galectin-3 gene expression patterns. In a total of 30 cases from papillary carcinoma, 21 cases from p53 (70%), 27 cases in galectin-3 (90%), and 26 cases in cyclin D1 (86.7%) showed positive rate. The galectin-3 staining investigated, showed a significant difference between a papillary carcinoma and a follicular carcinoma. Follicular carcinoma from 15 cases, p53 in 13 cases (86.7%), galectin-3 in 5 cases (33.3%) and cyclin D1 in 12 cases (80%) showed a positive rate. The cyclin D1 in follicular carcinoma and staining between the p53 that had correlation was also investigated. In this study, as the examples of the expression of the 27 cases of galectin-3 (90%) in papillary carcinoma and 5 cases in follicular carcinoma (33.3%) indicate, it was concluded that there is a difference in the expression on both carcinoma. In addition, cyclin D1 and p53 has a positive rate in follicular carcinoma, when cyclin D1 in 12 cases (80%), there was a significant correlation that was investigated. Distinguishing between papillary carcinoma and follicular carcinoma can be identified by the expression of galectin-3. It is considered to get results that are more accurate in follicular carcinoma diagnosis depending on whether the cyclin D1 and p53 is expressed or not.

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Isolation and Thermal Inactivation of Horseradish Peroxidase Isozymes (서양고추냉이에 있는 페르옥시다아제 이소짐의 분리(分離)와 열불활성화(熱不活性化))

  • Yoon, Jung-Ro;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.14 no.2
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    • pp.125-129
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    • 1982
  • Four peroxidase isozymes from horseradish roots (isozymes A, B, C and D) were isolated by chromatography and were thermally inactivated at $70{\sim}97^{\circ}C$ and pH 7.0. The four isozymes had different inactivation rates and the inactivation of each isozymes did not follow first order kinetics. D values of isozymes A, B, C, D and crude enzyme were 594s, 1850s, 2050s, 78s, 130s and z values were $24.0^{\circ}C$, $12.5^{\circ}C$, $18.0^{\circ}C$, $23.7^{\circ}C$ and $24.0^{\circ}C$, respectively. Sephadex gel chromatogram of the thermally treated isozyme C indicated that the shape and molecular weight of the native isozyme changed during inactivation.

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Studies on the Chemical Control of White-tip Nematode, Aphelenchoides besseyi Christie, before Transplanting (모내기전 벼이삭선충(Aphelenchoides besseyi Christie)의 약제방제에 관한 연구)

  • Lee Y. B.;Park J. S.;Han S. C.
    • Korean journal of applied entomology
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    • v.11 no.1
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    • pp.37-40
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    • 1972
  • Experiments were carried out to control white-tip nematode, Aphelenchoides besseyi Christie, during the period just before transplanting. The experimental results were summarized as fellows: 1: From a 12-hr to a 24-hr sled-soaking application into 500 times dilutions of Malathion $80\%$ Ec, Sumithion $50\%$ EC and Lebaycid $50\%$ EC were highly effective than other emulsifierble concentrations of chemicals. 2) The water surface application of PSP-204 $5\%$ G, Diazinon $3\%$ G, Nemagon $20\%$ G and Terracurr-P $5\%$ G were effective. 3: Root·dipping application of Sumithion $50\%$ Ec, Lebaycid $50\%$ Ec and Nemagon $80\%$ Ec were more effective chemicals than others.

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Influence of Ca-Na-Cl physicochemical solution properties on the adsorption of Se(-II) onto granite and MX-80 bentonite

  • Joshua Racette ;Andrew Walker ;Shinya Nagasaki ;Tianxiao Tammy Yang ;Takumi Saito ;Peter Vilks
    • Nuclear Engineering and Technology
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    • v.55 no.10
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    • pp.3831-3843
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    • 2023
  • The adsorption behaviour of Se(-II) onto granite and MX-80 bentonite in Ca-Na-Cl solutions has been studied utilizing adsorption experiments and surface complexation modelling. Adsorption kinetic experiments allude to steady-state adsorption periods after 7 days for granite and 14 days for MX-80 bentonite. Batch adsorption experiments were carried out to determine the influence that the physicochemical solution properties would have on Se(-II) adsorption behaviour. Adsorption of Se(-II) onto granite and MX-80 bentonite follows the trend of anionic adsorption, with a decrease in Rd values as the solution pH increased. There is also an ionic strength influence on the adsorption of Se(-II) onto granite with a decrease in the Rd value as the ionic strength increased. This effect is not found when observing Se(-II) adsorption onto MX-80 bentonite. Final experiments with a representative groundwater, determined that the adsorption of Se(-II) onto granite and MX-80 bentonite returned Rd values of (1.80 ± 0.10) m3·kg-1 and (0.47 ± 0.38) m3·kg-1, respectively. In support of the experiments, a surface complexation modelling approach has been employed to simulate the adsorption of Se(-II) onto granite and MX-80 bentonite, where it was determined that two different surface complexes, ≡S_Se- and ≡SOH2+_H2 were capable of simulating Se(-II) adsorption behaviour.

Regeneration and the Maturation Induction of Free-Living Gametophytes of a Kelp Saccharina sculpera (Phaeophyceae) (갈조류 개다시마 유리배우체의 재생 및 성숙 유도)

  • Yoo, Hyun Il;Lee, Ki Hyun;Kim, Soo Hong;Ha, Dong Soo;Hwang, Eun Kyoung
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.576-583
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    • 2018
  • Investigation of optimal temperature, irradiance and photoperiod conditions for free-living gametophytes of Saccharina sculpera for natural resources conservation and mass cultivation of endangered species in the eastern coast of Korea. Induction of regeneration and maturation of the free-living gametophytes of S. sculpera were cultured at temperatures (5, 10, 15, 20 and $25^{\circ}C$), irradiance (10, 20, 40, 60 and $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and photoperiods (14:10, 12:12 and 10:14 h L:D). The female gametophyte were actively regenerated without reaching sexual maturity under $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D) conditions. In contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $10{\mu}mol\;photons\;m^{-2}\;s^{-1}$ and 12:12 h (L:D). The sexual maturation of female and male gametophytes took place under $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14:10 h (L:D) and $10^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10:14 h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for conservation and utilization of S. sculpera.

Quality and Fruit Productivity of the Second Truss Blooming Seedlings Depending on Concentration of Nutrient Solution in Cherry Tomato (양액 농도에 따른 방울토마토 2화방 개화묘의 소질 및 과실 생산성)

  • Lee, Mun Haeng
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.230-236
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    • 2022
  • This study was carried out to produce two-flowered seedlings, harvest them early in a greenhouse, and extend the harvest period. This study was carried out to effectively produce the second truss blooming seedlings to harvest tomatoes early and extend the harvest period. For production of the second truss blooming seedlings (one stem), the nutrient solution EC was supplied at 1.5, 2.0, 2.5 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 60 days, which was 20-40 days longer than conventional seedlings, and 10 days longer than the first truss blooming seedlings (cube seedlings). The plant height was 78 and 77 cm in EC 2.5 dS·m-1 and dynamic management respectively, which was shorter than EC 1.5 dS·m-1 with 88 cm. As for the EC in the cube before formulation, dynamic management had the highest EC 5.5 dS·m-1, and the cube supplied with EC 1.5 dS·m-1 had the lowest. The production yield by treatment did not a difference among in the second truss blooming seedlings, but the first truss blooming seedlings showed lower productivity than second truss blooming seedlings. The second truss blooming seedling were harvested 35 days after planting on June 4, the first harvest date, and the first truss blooming were harvested in 42 days on June 11th. There was no difference in plant height and root growth due to bending at frequency planting. In the study on the production of the second truss blooming seedlings (two stem), the nutrient solution EC was supplied under 2.0, 2.5, 3.0 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 90 days, which was 40-50 days longer than conventional seedlings and 10 days longer than the first truss blooming seedlings (cube seedlings). Plant height was 80 and 81 cm in EC 2.0 dS·m-1 and 2.5 dS·m-1 respectively, but was the shortest at 73 cm in dynamic management. EC in the medium increased as the seeding period increased in all treatments. The dynamic management was the highest with EC 5.1 dS·m-1. There was no difference in yield among EC treatments in the second truss blooming seedlings, which had a longer seeding period of about 10 days, produced 15% more than the first truss blooming seedlings. In order to shorten the plant height of the second truss blooming seedlings, it is judged that the most efficient method is increasing the concentration of nutrient solution.

Individual Identification by Analysis of DNA from the Teeth (치아에서의 DNA 분석에 의한 개인식별)

  • Chang-Lyuk Yoon;Chong-Youl Kim
    • Journal of Oral Medicine and Pain
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    • v.20 no.1
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    • pp.229-246
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    • 1995
  • The deoxyribonucleic acid(DNA) was isolated from the pulp, dentin and enamel of the 4 fresh teeth and the 7 teeth left in room temperature for 10 years. Then it was examed to find out the usefulness for forensic dental medicine. Samples of the tooth-derived DNA amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologous amelogenin gene and D1S80 locus detection. The following results have been achieved. DNA extraction was possible in pulp and dentin of the fresh teeth, so it could be applicatable to detection of X-Y homologous amelogenin gene for sex determination, amplification of D1S80 locus by PCR. Sex determination was possible in pulp and dentin of the teeth left at room temperature for 10 years. Also, possible the detection fo AMP-FLPs to increase PCR cycling up to 40. DNA was isolated from all pulp of the fresh teeth and the teeth left in room temperature for 10 years, and also isolated from the dentin of the fresh teeth, partially isolated(3/7) from the dentin of the teeth left in room temperature for 10 years, but DNA was not isolated from enamel. From the above investigation, DNA extraction, sex determination, amplification of D1S80 locus were successfully accomplished even though the teeth were left for 10 years at room temperature. Therefore, teeth, especially pulp, are highly reliable and applicable as molecular biological samples for individual identification.

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Fabrication and characterization of 1.55$\mu$m SI-PBH DFB-LD for 10 Gbps optical fiber communications (10 Gb/s 급 광통신용 1.55$\mu$m SI-PBH DFB-LD의 제작 및 특성연구)

  • 김형문;김정수;오대곤;주흥로;박성수;송민규;곽봉신;김홍만;편광의
    • Korean Journal of Optics and Photonics
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    • v.8 no.4
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    • pp.327-332
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    • 1997
  • We fabricated the high speed 1.55${\mu}{\textrm}{m}$ distributed feedback laser diodes (DFB-LD) using both two-step mesa etching process and semi-insulating InP current blocking layers. The devices characteristics were threshold current of ~15mA, slope efficiency of ~0.13mW/mA, and dynamic resistance of ~6.0Ω, with as-cleaved facets. The fabricated DFB-LD showed the single longitudinal mode with more than 40dB up to 6 $I_{th}$(CW condition), emitting at the wavelength of 0.546${\mu}{\textrm}{m}$. The -3dB bandwidth was >10㎓ at the driving current of 27mA, and the maximum -3dB bandwidth was ~18㎓ at 90 mA current, showing the superior frequency response of SI-PBH DFB-LD. In the 10Gb/s transmission experiment for 1.55${\mu}{\textrm}{m}$ DFB-LD module, maximum 10 km of single mode fiber(SMF) or 80 km of dispersion shifted fiber (DSF) could be transmitted with error free.

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Susceptibility of the Alfalfa Weevil, Hypera postica (Coleoptera: Curculionidae) to Korean Entomopathogenic Nematodes in Laboratory Assays (한국산 곤충병원성 선충에 대한 알팔파바구미의 감수성 실내 검정)

  • Kim, Hyeong-Hwan;Han, Gun-Yeong;Park, Chung-Chan;Choo, Ho-Yul;Cho, Sung-Rae;Lee, Heung-Soo;Lee, Dong-Woon;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.147-151
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    • 2007
  • Laboratory tests were conducted to determine the susceptibility of the alfalfa weevil, Hypera postica to Korean entomopathogenic nematodes, Steinernema carpocapsae GSN1 strain (ScG), S. glaseri Dongrae strain (SgD), Heterorhabditis bacteriophora Hamyang strain (HbH), and Heterorhabditis sp. Gyeongsan strain (HeG) at the petri dish assay. The larval mortality of H. postica was significantly different depending on nematode species and treatment concentration. SgD and HeG strain were more effective against H. postica larva than ScG and HbH strain. When SgD and HeG strain were treated with the rate of >20 infective juveniles (ijs) per alfalfa weevil larva, mortality was ca. 77.5-100% at the late instars of H. postica in 3 days. The number of established nematode was significantly different depending on nematode species, whereas number of progeny was not significantly different. The mean number of established us of SgD strain in a host was the highest at 80 ijs by 30.2. The highest progeny number of HeG strain was 2,671.5 with 80 ijs. All nematode strains were not parasitic entirely to H. postica adults. These results show that alfalfa weevil late larva is highly susceptible to Korean entomopathogenic nematodes in the laboratory.