• 제목/요약/키워드: D1/D2 domain

검색결과 627건 처리시간 0.037초

Ectopic expression of $ARR1{\Delta}DDK$ in tobacco: alteration of cell fate in root tip region and shoot organogenesis in cultured segments

  • Rashid, Syeda Zinia;Kyo, Masaharu
    • Plant Biotechnology Reports
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    • 제4권1호
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    • pp.53-59
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    • 2010
  • A specific deleted version of ARABIDOPSIS RESPONSE REGULATOR1 (ARR1) lacking the signal receiver domain (1.152 amino acids)-coding sequence, referred to as $ARR1{\Delta}DDK$, was amplified using Arabidopsis thaliana cDNA prepared from adult leaves and transferred into the genome of Nicotiana tabacum cv. Samsun under the transcriptional control of a ${\beta}$-estradiol-inducible expression system. The ectopic expression of $ARR1{\Delta}DDK$ affected the morphology of transgenic seedlings and their segments in vitro. In the presence of an inducer, ${\beta}$-estradiol, ectopic expression of $ARR1{\Delta}DDK$ induced only the formation of soft, pseudo-bulbous tissue in the root tip region of intact seedlings, which appeared similar to callus generated on a hypocotyl segment in the presence of 2,4-D and 6-benzyladenine (BA), both at $1\;{\mu}M$. Those callus tissues on the root tip region could not generate shoots unless $1\;{\mu}M$ BA was supplied. In segment culture, ectopic expression of $ARR1{\Delta}DDK$ induced calluslike tissue around the cut-end of cotyledon and hypocotyl segments with occasional shoot formation, suggesting that the expression of $ARR1{\Delta}DDK$ could substitute for the effects of cytokinin on these segments. Additionally, treatment with only ${\beta}$-estradiol induced NtWUS, a WUS ortholog in tobacco, which was detected during the process of callus tissue formation in the root tip region and also in cotyledon or hypocotyl segments. These findings suggest that the NtWUS might be associated in the transdifferentiation process caused by the functional regulation of $ARR1{\Delta}DDK$ in transgenic tobacco seedlings.

전이학습 기반 특징융합을 이용한 누출판별 기법 연구 (A Study on Leakage Detection Technique Using Transfer Learning-Based Feature Fusion)

  • 한유진;박태진;이종혁;배지훈
    • 정보처리학회 논문지
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    • 제13권2호
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    • pp.41-47
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    • 2024
  • 시간 및 주파수 영역에서 각각 학습한 모델 간에 성능 차이가 발생할 경우, 앙상블을 수행하더라도 개별 모델 간의 성능 불균형으로 인하여 앙상블의 성능이 오히려 저하되는 현상을 확인할 수 있었다. 따라서, 본 논문은 시간 영역과 주파수 영역에서 특징을 추출하고, 이들을 융합한 단계적 학습 방법을 통해 파이프라인 누출 감지의 정확성을 높이기 위한 누출판별 기법을 제안한다. 이 방법은 두 단계의 학습 과정으로 이루어지며, 먼저, 단계 1에서는 시간 영역과 주파수 영역에서 독립적으로 모델 학습을 수행하여 도메인별로 주어진 데이터로부터 중요한 특징들을 효과적으로 추출하도록 하였다. 단계 2에서는 사전학습 완료된 각 모델로부터 해당 분류기를 제거한 후, 두 도메인의 특징들을 서로 융합하고 새로운 분류기를 추가하여 재학습을 수행하였다. 본 논문에서 제안하는 전이학습 기반 특징융합 기법은 시간 및 주파수 영역에서 추출된 특징들을 융합하여 모델 학습을 수행함으로써, 두 영역의 특징이 상호 보완적으로 작용하여 모델이 다양한 정보를 활용함으로 인해 99.88%의 높은 정확도를 달성하여 파이프 누수 감지에 있어 우수한 성능을 입증하였다.

문헌조사법의 내용구조에 관한 고찰 (A study on content structure of literature search methodology)

  • 김정소;박준식
    • 한국도서관정보학회지
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    • 제10권
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    • pp.27-58
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    • 1983
  • The purpose of this study is to clarify the educational literature search methodology objectives and the content structure of a course in bibliography. The content of this study can be summarized as follows: 1. The concept and domain of literature search methodology, which has traditionally gone by the name of a guide to use of literature and library, are described. 2. The need of incorporating the methodology into regular college curriculum was investigated in view of the ideology of college education. 3. The background, process, and current status of offering a course in the methodology as a part of regular college curriculum were examined. 4. The educational objective of a course in the methodology was set at the successful activities in self study, life-long-education, and special topic research. 5. Considering the fact that technical knowledge and propositional knowledge should be realized simultaneously, that educational experience should go hand in hand with materials and library, and that knowledge should be constructed systematically and logically, the content structure of the methodology were divided into the following six categories: a) the significance of the methodology of literature search b) the college library and the activities of literature search c) types and characteristics of materials d) literature search by catalog e) explanation of basic bibliography f) research and literature search

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Structural Change in Transmembrane Region of Syndecan-4 by Mutation

  • Choi, Sung-Sub;Kim, Ji-Sun;Jeong, Ji-Ho;Kim, Yongae
    • 한국자기공명학회논문지
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    • 제20권4호
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    • pp.129-137
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    • 2016
  • Transmembrane(TM) proteins are closely related to transport, channel formation, signaling, cell to cell interaction, so they are the crucial target of modern medicinal drugs. In order to study the structure and function of these TM proteins, it is important to prepare reasonable amounts of proteins. However, their preparation is seriously difficult and time-consuming due to insufficient yields and low solubility of TM proteins. We tried to produce large amounts of Syndecan-4 containing TM domain(SDC4-TM) that is related to the wound healing and tumor. Also, mutated SDC4-TM was studied to investigate structural change by modification of dimerization motif. We performed the structure determination by the Polarity Index at Slanted Angle (PISA) wheel pattern analysis based on $^{15}N-^1H$ 2D SAMPI-4 solid-state NMR of SDC4-TM and computational modeling using Discovery Studio 2016.

자장열처리시킨 Fe기 비정질합금의 자기적성질과 자구구조 (Magnetic Properties and Domain structures of Fe-based Amorphous Alloys with Magnetic Annealing)

  • 김태호;정광호;송진태
    • E2M - 전기 전자와 첨단 소재
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    • 제1권4호
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    • pp.319-332
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    • 1988
  • 높은 포화자속밀도와 낮은 철손을 갖는 Fe/aub 80/B$_{12}$Si$_{8}$ 비정질합금을 일반열처리, 자장열처리시켜 그의 자기적특성과 자구구조와의 관계를 조사하였다. 이를 위하여 Fe$_{80}$B$_{12}$Si$_{8}$ 비정질리본을 단롤법으로 제작하여 결정화온도를 측정하였으며 측정된 결정화온도 이하의 여러 온도에서 30분간 Ar-gas 분위기하에서 일반열처리, 자장열처리를 행하였다. 이와같이 하여 준비된 시료의 자기적특성을 조사하기 위하여 D.C., A.C. Recorking Fluxmeter를 이용하였으며 자구구조는 Bitter method로 관찰하였다. as-cast 상태의 시료를 일반열처리함에 따라 내부응력이 완화되면서 maze자구가 점차 사라지고 wave형태의 180.deg.자구가 관찰되었다. 동시에 자화과정에 있어서 자기이력곡선은 Barkhausen jump가 없어 smooth하였다. 그리고 자장열처리시에는 as-cast 상태나 일반열처리에 비해 자기적특성이 현저하게 향상되었으며 이는 열처리를 행함에 따라 내부응력이 완화되면서 maze 자구가 없어지고 일축자기 이방성으로 리본길이방향에 평행하게 형성된 180.deg.자구에 기인하는 것이라 사료된다. 그리고 자장열처리의 경우, 폭방향으로 열처리한 리본의 자구폭은 길이방향으로 열처리한 리본의 폭보다 미세하였으며 전자의 이력손실이 후자의 것보다 더 컸다.다.

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ANALYSIS OF A LAMINATED COMPOSITE WIND TURBINE BLADE CHARACTERISTICS THROUGH MATHEMATICAL APPROACH

  • CHOI, YOUNG-DO;GO, JAEGWI;KIM, SEOKCHAN
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • 제23권4호
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    • pp.367-380
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    • 2019
  • A 1kW-class horizontal axis wind turbine (HAWT) rotor blade is taken into account to investigate elastic characteristics in 2-D. The elastic blade field is composed of symmetric cross-ply laminated composite material. Blade element momentum theory is applied to obtain the boundary conditions pressuring the blade, and the plane stress elasticity problem is formulated in terms of two displacement parameters with mixed boundary conditions. For the elastic characteristics a fair of differential equations are derived based on the elastic theory. The domain is divided by triangular and rectangular elements due to the complexity of the blade configuration, and a finite element method is developed for the governing equations to search approximate solutions. The results describe that the elastic behavior is deeply influenced by the layered angle of the middle laminate and the stability of the blade can be improved by controlling the layered angle of laminates, which can be evaluated by the mathematical approach.

Quaternion Markov Splicing Detection for Color Images Based on Quaternion Discrete Cosine Transform

  • Wang, Jinwei;Liu, Renfeng;Wang, Hao;Wu, Bin;Shi, Yun-Qing
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제14권7호
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    • pp.2981-2996
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    • 2020
  • With the increasing amount of splicing images, many detection schemes of splicing images are proposed. In this paper, a splicing detection scheme for color image based on the quaternion discrete cosine transform (QDCT) is proposed. Firstly, the proposed quaternion Markov features are extracted in QDCT domain. Secondly, the proposed quaternion Markov features consist of global and local quaternion Markov, which utilize both magnitude and three phases to extract Markov features by using two different ways. In total, 2916-D features are extracted. Finally, the support vector machine (SVM) is used to detect the splicing images. In our experiments, the accuracy of the proposed scheme reaches 99.16% and 97.52% in CASIA TIDE v1.0 and CASIA TIDE v2.0, respectively, which exceeds that of the existing schemes.

모드 연성을 수반하는 분할 모형의 비틀림 감쇠비 추정 (Torsional Damping Estimation of a Segmented Hull Model with Modal Coupling)

  • 김유일;박성건
    • 대한조선학회논문집
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    • 제53권6호
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    • pp.482-493
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    • 2016
  • The identification of modal damping of a segmented hull model with torsional response is difficult task due to the coupling of modal response. This is because the 1st and 2nd torsional vibration modes are closely spaced in frequency domain leading to the situation that the modal decomposition is difficult to achieve by simple band-pass filter. Present study applied several different modal decomposition methods to derive the damping ratio of different modes. The modal decomposition methods considered in this study are simple band-pass filter, Hilbert vibration decomposition, Wavelet transform and proper orthogonal decomposition. Coupled free decay signal obtained from the torsional hammering test on a segmented hull model was processed with four different methods and the derived damping ratios were compared with each other. Discussions also have been made on the pros and cons of the different methodologies.

Current overshoot operation of a REBCO magnet to mitigate SCF

  • Lee, Changhyung;Hahn, Seungyong;Bang, Jeseok;Cho, Jeonwook;Kim, Seokho
    • 한국초전도ㆍ저온공학회논문지
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    • 제20권4호
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    • pp.65-69
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    • 2018
  • Due to large in-field current carrying capacity and strong mechanical strength, a REBCO wire has been regarded as a viable high temperature superconductor (HTS) option for high field MRI and > 1 GHz (>23.5 T) NMR magnets. However, a REBCO magnet is well known to have an inherent problem of field inhomogeneity, so-called 'Screening Current induced magnetic Field (SCF)'. Recently, 'field shaking' and 'current overshoot operation' techniques have been successfully demonstrated to mitigate the SCF and enhance the field homogeneity by experiments. To investigate the effectiveness of current overshooting operation technique, a numerical simulation is conducted for a test REBCO magnet composed of a stack of double pancake coils using '2D edge-element magnetic field formulation' combined with 'domain homogenization' scheme. The simulation result demonstrates that an appropriate amount of current overshoot can negate the SCF. To verify the simulation results, current overshoot experiments are conducted for the REBCO magnet in liquid nitrogen. Experimental results also demonstrate the possible application of current overshoot technique to mitigate the SCF and enhance the field homogeneity.

Wake dynamics of a 3D curved cylinder in oblique flows

  • Lee, Soonhyun;Paik, Kwang-Jun;Srinil, Narakorn
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제12권1호
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    • pp.501-517
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    • 2020
  • Three-dimensional numerical simulations were performed to study the effects of flow direction and flow velocity on the flow regime behind a curved pipe represented by a curved circular cylinder. The cylinder is based on a previous study and consists of a quarter segment of a ring and a horizontal part at the end of the ring. The cylinder was rotated in the computational domain to examine five incident flow angles of 0-180° with 45° intervals at Reynolds numbers of 100 and 500. The detailed wake topologies represented by λ2 criterion were captured using a Large Eddy Simulation (LES). The curved cylinder leads to different flow regimes along the span, which shows the three-dimensionality of the wake field. At a Reynolds number of 100, the shedding was suppressed after flow angle of 135°, and oblique flow was observed at 90°. At a Reynolds number of 500, vortex dislocation was detected at 90° and 135°. These observations are in good agreement with the three-dimensionality of the wake field that arose due to the curved shape.