• Title/Summary/Keyword: D-loop region

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우리나라 긴꼬리닭의 계통분류학적 추정

  • Yeon, Seong-Heum;Jo, Chang-Yeon;Kim, Jong-Dae;Jin, Hyeon-Ju;Lee, Seung-Su;Kim, Yeong-Geun;Sang, Byeong-Don
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2006.11a
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    • pp.84-85
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    • 2006
  • This study was carried out to ascertain phylogenetic status of long-tail chicken which found recently in Korea and was presumed to be a kind of Korean Natives. 10 loci microsatellites were analysed for 449 birds of 11 groups and 2 region of mitochondrial DNA were sequenced for 135 birds of the same groups, that consist of 3 introduced breeds and 8 Korean Natives including 3 long-tail chicken. In mean numbers of alleles per locus(MNA) for microsatellites, long-tail chicken were smaller (2.60${\sim}$3.20) than the others, but in heterozygosities, were higher(0.4087${\sim}$0.5375) than others that were the same level of MNA. And in the neighbor joining bootstrap tree drawing by Nei's standard distance, they made a cluster with some Korean Native groups. All of the nucleotide sequences of mitochondrial cytochrome b gene and D-loop were classified into 23 haplotypes. In long-tail chicken, the haplotypes were 3 kinds, and were different among the groups (LTA, LTB and LTD). Resultly, it was supposed that 3 groups of the long-tail chicken be all a kind of Korean Natives.

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Multiple Maternal Lineages of Vietnamese Local Chickens Inferred by Mitochondrial DNA D-loop Sequences

  • Cuc, Ngo Thi Kim;Simianer, Henner;Groeneveld, Linn Fenna;Weigend, Steffen
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.2
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    • pp.155-161
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    • 2011
  • In this study, mitochondrial DNA (mtDNA) sequence polymorphism was used to assess genetic diversity of nine Vietnamese local chicken breeds. In addition, two Chinese breeds kept in Vietnam were included in the analysis for comparison. A 455-bp fragment of the mtDNA D-loop region was sequenced in 222 chickens of these 11 breeds. As reference, a skeleton was constructed based on chicken mtDNA sequences taken from the Genbank. Haplotypes of the nine Vietnamese local and two Chinese breeds were aligned together with these sequences. The Vietnamese and Chinese breeds showed a high degree of variability. In total, 37 haplotypes were identified in the chicken breeds studied forming eight clades. Thereby, the majority of individuals of the two Chinese breeds grouped together in one clade which is assumed to have its roots in the Indian subcontinent. Although the Vietnamese chicken breeds were distributed across all eight clades, most of them clustered in three main clades. These results suggest that the Vietnamese domestic chickens have originated from multiple maternal lineages, presumably from Yunnan and adjacent areas in China, South and Southwest China and/or surrounding regions (i.e. Vietnam, Burma, Thailand, and India).

A Basic Helix-Loop-Helix Transcription Factor Regulates Cell Elongation and Seed Germination

  • Kim, Jin-A;Yun, Ju;Lee, Minsun;Kim, Youn-Sung;Woo, Jae-Chang;Park, Chung-Mo
    • Molecules and Cells
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    • v.19 no.3
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    • pp.334-341
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    • 2005
  • Plants are sessile and rely on a wide variety of growth hormones to adjust growth and development in response to internal and external stimuli. We have identified a gene, designated NAN, encoding a basic helix-loop-helix (bHLH) transcription factor that regulates cell elongation and seed germination in plants. NAN has an HLH motif in its C-terminal region but does not have any other discernible homologies to bHLH proteins. A bipartite nuclear localization signal is located close to the HLH motif. An Arabidopsis mutant, nan-1D, in which NAN is activated by the insertion of the 35S enhancer, exhibits growth retardation with short hypocotyls and curled leaves. It is also characterized by reduced seed germination and apical hook formation, symptomatic of GA deficiency or disrupted GA signaling. The phenotypic effects of nan-1D were increased by treatment with paclobutrazol (PAC), an inhibitor of gibberellic acid (GA) biosynthesis. NAN is constitutively expressed throughout the life cycle. Our observations indicate that NAN has a housekeeping role in plant growth and development, particularly in seed germination and cell elongation, and that it may modulate GA signaling.

3-D Structure of a Coronal Jet Seen in Hinode, SDO, and STEREO

  • Lee, Kyoung-Sun;Innes, Davina;Moon, Yong-Jae;Shibata, Kazunari
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.89.1-89.1
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    • 2011
  • We have investigated a coronal jet near the limb on 2010 June 27 by Hinode/X-Ray Telescope (XRT), EUV Imaging Spectrograph (EIS), Solar Optical Telescope (SOT), SDO/Atmospheric Imaging Assembly (AIA), and STEREO. From EUV (AIA and EIS) and soft X-ray (XRT) images we identify the erupting jet feature in cool and hot temperatures. It is noted that there was a small loop eruption in Ca II images of the SOT before the jet eruption. Using high temporal and multi wavelength AIA images, we found that the hot jet preceded its associated cool jet. The jet also shows helical-like structures during the rising period. According to the spectroscopic analysis, the jet structure changes from blue shift to red one with time, implying the helical structure of the jet. The STEREO observation, which enables us to observe this jet on the disk, shows that there was a dim loop associated with the jet. Comparing the observations from the AIA and STEREO, the dim loop corresponds to the jet structure which implies the heated loop. Considering that the structure of its associated active region seen in STEREO is similar to that in AIA observed 5 days before, we compared the jet morphology on the limb with the magnetic fields extrapolated from a HMI vector magnetogram observed on the disk. Interestingly, the comparison shows that the open field corresponds to the jet which is seen as the dim loop in STEREO. Our observations (XRT, SDO, SOT, and STEREO) are well consistent with the numerical simulation of the emerging flux reconnection model.

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Genetic Distribution Pattern of Bluegill Sunfish Lepomis macrochirus in Freshwater Ecosystems across Korea

  • Lau, Hwee Hui;Huang, Jingting;Kwan, Ye-Seul;Lee, Wan-Ok;Won, Yong-Jin
    • Animal Systematics, Evolution and Diversity
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    • v.25 no.3
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    • pp.325-329
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    • 2009
  • Lepomis macrochirus from the family Centrarchidae, commonly known as Bluegill sunfish, is an introduced freshwater fish in Korea that thrives in lakes, ponds, reservoirs and rivers. Since its introduction into Korea in 1969, Lepomis macrochirus has rapidly dispersed out and increased in number almost all over the freshwater ecosystems in Korea. Consequently this species causes a severe ecological problem, threatening native fishes due to its omnivorous foraging behaviors upon fish juveniles and many freshwater invertebrates. To address population genetic structure of L. macrochirus, 74 fish samples from 10 populations were collected and compared for their mitochondrial D-loop control region. As the result we found that the genetic diversity of L. macrochirus is extremely low such as resulting only four haplotypes with a few nucleotide differences among them. Analysis of molecular variance (AMOVA) revealed that the source of population genetic variation is largely retained in the comparisons among individuals within populations, while it is relatively low with slight significance at the highest hierarchical group. This distribution pattern differs from what is expected when biogeography is under the influence of natural geographic barriers such as mountain ranges in Korea. Instead the result is accord with the influential role of random spreading events facilitated by local people for aquaculture and fishing, and subsequent dispersals since its single point of introduction into Korea.

Redescription of Free-Living Marine Nematode, Dracograllus filipjevi Allen and Noffsinger, 1978 (Nematoda: Draconematidae) from Korea

  • Rho, Hyun-Soo;Kim, Dong-Sung;Kim, Won
    • Ocean Science Journal
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    • v.41 no.3
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    • pp.163-173
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    • 2006
  • A draconematid nematode species, Dracograllus filipjevi Allen and Noffsinger, 1978, is described from the washings of shallow littoral coralline algae at Guryongpo in the eastern coast of Korea. This species is recognized by the following characteristics: larger body size, number of posterior sublateral adhesion tubes (9 in male and 12-13 in female) and subventral adhesion tubes (8-9 in male and 9-10 in female), the absence of cephalic acanthiform setae on rostrum, the presence of longitudinally areolated body cuticle with dot-like punctations, the presence of some cuticular collar in swollen pharyngeal region, the shape of amphidial fovea (large, elongate, and loop-shaped in both sexes), the absence of preanal corniform setae, and the absence of lateral differentiation on narrow body region. Morphological features of the species using differential interference contrast photomicrographs are described in detail in the present work. The is the first report of D. filipjevi in Korea.

Higher Order Structure of 5S rRNA from Xanthomonas palargonii (Xanthomonas palargonii 5S rRNA의 고차원 구조)

  • Cho, Bongrae;Kim, Sang Bumn;Lee, Younghoon;Park, Inwon
    • Journal of the Korean Chemical Society
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    • v.39 no.9
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    • pp.734-740
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    • 1995
  • The primary and secondary structures of Xanthomonas palargonii 5S rRNA were determined. The higher order structure of the 5S rRNA was also analysed using several ribonucleases and chemical probes, such as ethylnitrourea, Pb2+, dimethylsulfate and diethyl pyrocarbonate. Ethylnitrourea was used for probing the phosphate groups involved in the tertiary interactions in the 5S rRNA. Nucleotides G72, A73, G75, A78, G98, G100 and A101 in unstable helical region d, nucleotides C36, C37, A39, and C41 in loop c, nucleotides A29 and G33 in stem C became resistant to ethylnitrourea modification in the presence of Mg2+. On the basis of findings from chemical and enzymatic studies, and also Pb2+-induced cleavages, it was concluded that region b-C and unstable helical region d may act as hinges in the folding of 5S rRNA.

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Sensorless Control of Non-salient Permanent Magnet Synchronous Motor Drives using Rotor Position Tracking PI Controller

  • Lee Jong-Kun;Seok Jul-Ki
    • KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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    • v.5B no.2
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    • pp.189-195
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    • 2005
  • This paper presents a new velocity estimation strategy for a non-salient permanent magnet synchronous motor drive without high frequency signal injection or special PWM pattern. This approach is based on the d-axis current regulator output voltage of the drive system, which contains the rotor position error information. The rotor velocity can be estimated through a rotor position tracking PI controller that controls the position error at zero. For zero and low speed operation, the PI gain of the rotor position tracking controller has a variable structure according to the estimated rotor velocity. Then, at zero speed, the rotor position and velocity have sluggish dynamics because the varying gains are very low in this region. In order to boost the bandwidth of the PI controller during zero speed, the loop recovery technique is applied to the control system. The PI tuning formulas are also derived by analyzing this control system by frequency domain specifications such as phase margin and bandwidth assignment.

Design of H_{\infty} Control for Uncertain Linear Systems with Eigenvalue Assignment Constraint in a Disk (원판내 고유치 배정 조건을 갖는 불확정성 선형 시스템의H_{\infty}제어기 설계)

  • Ma, Sam-Seon;Kim, Jin-Hun
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.49 no.9
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    • pp.520-525
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    • 2000
  • This paper deals with the design of H$\infty$ control for uncertain linear systems with the regional eigenvalue assignment constraint. The considered region is a disk in the left half plane and the two types of time-varying uncertainties are considered. We presents a state feedback control that minimize the L2 gain from the disturbance to the measured output as well as it guarantees that all eigenvalues of closed loop are inside a disk. The state feedback control is obtained by checking the feasibility of linear matrix inequalities (LMI's) which are numerically tractable. Finally we give an example to show the applicability and usefulness of our results.

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Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele

  • Cho, Eunjoo;Lee, Euna;Kim, Eun Young
    • BMB Reports
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    • v.49 no.11
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    • pp.587-589
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    • 2016
  • The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657-707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in $Clk^{out}$ genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons ($LN_vs$) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues.