• Journal of Life Science
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• v.14 no.2
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• pp.325-330
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.

• Geophysics and Geophysical Exploration
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• v.20 no.2
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• pp.88-95
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• 2017

Chogye basin, which is surrounded by country rock, has a closed-basin form. In such a basin, incident seismic energy can form multiply reflected waves, thus causing energy concentration to occur at this closed-basin area. Microtremor measurement survey was performed at the Chogye basin, which is located in Chogye-myeon and Jeokjungmyeon, Hapcheon-gun, Gyeongsangnam-do, Republic of Korea. Microtremor data were transformed into the frequency domain, and then the horizontal-to-vertical spectral ratios (HVSR) were calculated. Fundamental resonance frequencies were estimated from the HVSR results for every observation point. Using the empirical relationship between site period and thickness for sediment sites in Korea known from the previous study, the distribution of sediment thickness of the Chogye basin was estimated from the fundamental resonance frequencies. Being compared with the mountainous rim with steep slope, the measurement points inside the basin have low values of the fundamental resonance frequency with the minimum of 1.03 Hz, which corresponds to the thickness of sedimentary layer with the maximum depth of about 100 m. A three-dimensional basin model was constructed for bedrock topography of the Chogye basin by an interpolation of basin depths estimated at each measurement site.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.19 no.4 s.74
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• pp.429-440
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• 2006

This paper comprises two specific objectives. The first is to examine the applicability of ordinary kriging interpolation(OK) to the p-adaptivity of the finite element method that is based on variogram modeling. The second objective Is to present the adaptive procedure by the hierarchical p-refinement in conjunction with a posteriori error estimator using the modified S.P.R. (superconvergent patch recovery) method. The ordinary kriging method that is one of weighted interpolation techniques is applied to obtain the estimated exact solution from the stress data at the Gauss points. The weight factor is determined by experimental and theoretical variograms for interpolation of stress data apart from the conventional interpolation methods that use an equal weight factor. In the p-refinement, the analytical domain has to be refined automatically to obtain an acceptable level of accuracy by increasing the p-level non-uniformly or selectively. To verify the performance of the modified S.P.R. method, the new error estimator based on limit value has been proposed. The validity of the proposed approach has been tested with the help of some benchmark problems of linear elastic fracture mechanics such as a centrally cracked panel, a single edged crack, and a double edged crack.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.9
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• pp.1777-1787
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• 2017

Federated authentication (FA) is a multi-domain authentication and authorization infrastructure that enables users to access nationwide R&D resources with their home-organizational accounts. An FA-enabled user is redirected to his/her home organization, after selecting the home from an identity-provider (IdP) discovery service, to log in. The discovery service allows a user to search his/her home from all FA-enabled organizations. Users get troubles to find their home as federation size increases. Therefore, a discovery service has to provide an intuitive way to make a fast IdP selection. In this paper, we propose a discovery system which leverages geographical information. The proposed system calculates geographical proximity and text similarity between a user and organizations, which determines the order of organizations shown on the system. We also introduce a server redundancy and a status monitoring method for non-stop service provision and improved federation management. Finally, we deployed the proposed system in a real service environment and verified the feasibility of the system.

• Advanced Composite Materials
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• v.17 no.1
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• pp.25-40
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• 2008

The research and development in soundproof materials for preventing noise have attracted great attention due to their social impact. Noise insulation materials are especially important in the field of soundproofing. Since the insulation ability of most materials follows a mass rule, the heavy weight materials like concrete, lead and steel board are mainly used in the current noise insulation materials. To overcome some weak points in these materials, fiber reinforced composite materials with lightweight and other high performance characteristics are now being used. In this paper, innovative insulation sheet materials with carbon and/or glass fabrics and nano-silica hybrid PU resin are developed. The parameters related to sound performance, such as materials and fabric texture in base fabric, hybrid method of resin, size of silica particle and so on, are investigated. At the same time, the wave analysis code (PZFlex) is used to simulate some of experimental results. As a result, it is found that both bundle density and fabric texture in the base fabrics play an important role on the soundproof performance. Compared with the effect of base fabrics, the transmission loss in sheet materials increased more than 10 dB even though the thickness of the sample was only about 0.7 mm. The results show different values of transmission loss factor when the diameters of silica particles in coating materials changed. It is understood that the effect of the soundproof performance is different due to the change of hybrid method and the size of silica particles. Fillers occupying appropriate positions and with optimum size may achieve a better effect in soundproof performance. The effect of the particle content on the soundproof performance is confirmed, but there is a limit for the addition of the fillers. The optimization of silica content for the improvement of the sound insulation effect is important. It is observed that nano-particles will have better effect on the high soundproof performance. The sound insulation effect has been understood through a comparison between the experimental and analytical results. It is confirmed that the time-domain finite wave analysis (PZFlex) is effective for the prediction and design of soundproof performance materials. Both experimental and analytical results indicate that the developed materials have advantages in lightweight, flexibility, other mechanical properties and excellent soundproof performance.

• Geophysics and Geophysical Exploration
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• v.18 no.1
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• pp.1-8
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• 2015

To estimate the shear-wave velocity (${\nu}_s$ beneath the OTVZ seismic station on Ngauruhoe volcano in New Zealand, we calculated receiver functions (RFs) using 127 teleseismic data ($Mw{\geq}5.5$) with high signal-to-noise ratios recorded during November 11, 2011 to September 11, 2013. The genetic inversion algorithms was applied to 21 RFs calculated by the iterative time-domain deconvolution method. In the 1-D ${\nu}_s$ model derived by the inversion, the Moho is observed at a 14 km depth, marked by a ${\nu}_s$ transition from 3.7 km/s to 4.7 km/s. The average ${\nu}_s$ of the overlying crust is 3.4 km/s, and the average ${\nu}_s$ of a greater than 9-km thick low-velocity layer (LVL) in the lower crust is 3.1 km/s. The LVL becomes thinner with increasing distance from the station. Another LVL thicker than 10 km with ${\nu}_s$ less than 4.3 km/s is found in the upper mantle. Those LVLs in the lower crust and the upper mantle and the relatively thin crust might be related to the magma activity caused by the subducting Pacific plate.

• The Korean Journal of Physiology
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• v.30 no.1
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• pp.43-51
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• 1996

This study was aimed to elucidate the changes in heart rate variability during treadmill exercise at various speeds and grades by spectral analysis. Thirty-three untrained male college students aged $20{\sim}26\;yr $were employed to exercise on a treadmill using 4 speeds (4.02, 5.47, 6.76 and 8.05 km/h) and 6 grades (0, 4, 8, 12, 16 and 20%). A fixed speed was selected for each session with the grade increased every 3 min. The electrocardiogram, respiration and the stepping activity were continuously recorded through an A/D converter system on the computer disk. Power spectra of heart rate variability (RRV) were obtained by use of a fast Fourier transform algorithm. The frequency domain was divided into 3 bands: $VLF\;(0{\sim}0.04\;Hz),\;LF\;(0.04 {\sim}0.15\;Hz)\;and\;HF\;(0.15{\sim}1.00\;Hz).$ Heart rate was $74.4{\pm}2.1\;beats/min$ at rest and showed a steady increase during treadmill exercise with increasing speed and grade up to $196.7{\pm}5.0\;beats/min.$ Total power of HRV was $35.0{\pm}6.7\;(beats/min)^{2}$ at rest and progressively decreased during exercise down to $1.9{\pm}0.3\;(beats/min)^{2}.$ The %VLF power of HRV was $34.5{\pm}3.7\; %$ at rest and showed no significant change during exercise except for a decrease observed at the highest intensity of exercise. The %LF power was $44.1{\pm}3.0\;%$ at rest and showed a progressive decrease down to $4.5{\pm}1.0\;%$ during those stages of exercise where heart rate was over 135 beats/min. The %HF power was $21.4{\pm}2.9\;%$ at rest and showed a progressive increase up to $87.1{\pm}6.7\;%$ during higher intensity exercise where heart rate was over 165 beats/min. Peak frequency of HF band was $0.200{\pm}0.018\;Hz$ at rest and was shifted to higher frequencies up to $0.909{\pm}0.048\;Hz$ at heart rates greater than 135 beats/min. Respiratory frequency was $18.0{\pm}1.5$ breaths/min at rest and significantly increased during exercise up to $53.0{\pm}3.7$ breaths/min. Stride frequency during treadmill exercise showed an increasing tendency with increasing speed from $55.6{\pm}0.9$ steps/min at 4.02 km/h to $81.2{\pm}0.6$ at 8.05 km/h. It was concluded that total power of HRV decreased progressively with increasing exercise intensity due to the withdrawal of parasympathetic activity. At higher exercise intensity, % LF power decreased and %HF power increased with its peak frequency shifted to higher values in a progressive mode with increasing speed and grade, reflecting a readjustment in the cardiovascular system and the increased respiration and its rate, respectively.

• Journal of the Korean Society of Safety
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• v.18 no.2
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• pp.132-138
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• 2003

The use of long rails in high-speed railway bridges causes additional stresses due to nonlinear behaviours between the rail and bridge decks in the neighbourhood of the deck joints. In the seismic response analysis of high-speed railway bridges, since structural response is highly sensitive to properties of the ground motion, spatial variation of the ground excitation affects responses of the bridges, which in turn affect stresses in the rails. In addition, it is shown that high-speed trains need very long distances to stop when braking under seismic occurrence corresponding to operational earthquake performance level so that verification of the safe stoppage of the train is also required. In view of such additional stresses due to long rails, sensibility of structural response to the properties of the ground motion and braking distance needed by the train to stop safely, this paper proposes and establishes a time domain nonlinear dynamic analysis method that accounts for braking loads, spatial variation of the ground motion and material nonlinearities of rails to analyze long rail stresses in high-speed railway bridges subjected to seismic event. The accuracy of the proposed method is demonstrated through an application on a typical site of the Korean high-speed railway.

• Journal of Intelligence and Information Systems
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• v.15 no.2
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• pp.33-48
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• 2009

Capsule endoscopy is one of the most remarkable inventions in last ten years. Causing less pain for patients, diagnosis for entire digestive system has been considered as a most convenience method over a normal endoscope. However, it is known that the diagnosis process typically requires very long inspection time for clinical experts because of considerably many duplicate images of same areas in human digestive system due to uncontrollable movement of a capsule endoscope. In this paper, we propose a method for clinical diagnosticians to get highly valuable information from capsule-endoscopy video. Our software system consists of three global maps, such as movement map, characteristic map, and brightness map, in temporal domain for entire sequence of the input video. The movement map can be used for effectively removing duplicated adjacent images. The characteristic and brightness maps provide frame content analyses that can be quickly used for segmenting regions or locating some features(such as blood) in the stream. Our experiments show the results of four patients having different health conditions. The result maps clearly capture the movements and characteristics from the image frames. Our method may help the diagnosticians quickly search the locations of lesion, bleeding, or some other interesting areas.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.2
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• pp.254-262
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• 2016

With the increased demand for automotive radar systems, mutual interference between vehicles has become a crucial issue that must be resolved to ensure better automotive safety. Mutual interference between frequency modulated continuous waveform (FMCW) radar system appears in the form of increased noise levels in the frequency domain and results in a failure to separate the target object from interferers. The traditional fast fourier transform (FFT) algorithm, which is used to estimate the beat frequency, is vulnerable in interference-limited automotive radar environments. In order to overcome this drawback, we propose a high-resolution frequency estimation technique for use in interference environments. To verify the performance of the proposed algorithms, a 77GHz FMCW radar system is considered. The proposed method employs a high-resolution algorithm, specially the multiple signal classification and estimation of signal parameters via rotational invariance techniques, which are able to estimate beat frequency accurately.