• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.657-663
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• 2002

Probiotics have been suggested to improve gastrointestinal health in humans. To investigate the effects of Bacillus polyfermenticus SCD administration on fecal microflora and putrefactive metabolites in humans, Bacillus polyfermenticus SCD (4.00${\times}$10$\sub$5/ CFU/mg) was administrated to ten healthy subjects (5 men and 5 women, average age 24 years) three times a day for 2 weeks. Fecal samples were collected before (1st and 2nd weeks, control), during (3rd and 4th weeks), and 2 weeks after the administration. The fo11owing microbial groups were evaluated in the feces: aerobic and anaerobic bacteria, Bacillus polyfermenticus SCD, Lactobacillus, Bifidobacterium, total lactic acid bacteria, Salmonella, Clostridium, Clostridium perfringens, Eubacterium, Staphylococcus, Coliform bacteria, Pseudomunas, and Yeast. Fecal concentrations of total aerobic bacteria (p<0.05, p<0.01, 3rd and 4th weeks), total lactic acid bacteria (p<0.01, 3rd, 4th and 5th weeks), and Bifidobacteria (p<0.05, 4th and 5th weeks) were significantly increased in all subjects, compared to the control, from the 3rd week after the administration of the products. Clostridium (p<0.01, 4th week), Clostridium perfringens (p<0.05, p<0.01, 3rd and 4th weeks), and coliform (p<0.01,5th week) were significantly reduced from the 3rd week of administration. No significant changes in the fecal concentrations of Pseudomonas, Lactobacillus, Eubacterium, Staphylococcus, yeast, and total anaerobes were observed. Six weeks after the administration, the concentration of all rnicroorganlsrns returned to the basal level. Bacillus polyfermenticus SCD was significantly maintained from the 3rd week to 6th week of the study. Despite the absence of a statistical significance, the putrefactive metabolites (ammonia, indole, skatole, and $\rho$-cresol) and the pH value tended to be lower during and after the test periods than the base line. These results show that this probiotic preparation is able to colonize the intestine, and suggest that it may be useful as a beneficial probiotic in humans.

• Korean Journal of Weed Science
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• v.4 no.1
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• pp.4-10
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• 1984

This experiment was conducted to determine the change in weed flora with season and cultivated crop and land. There are 44 species in 38 genera and 18 families, consisting of 22 annual, 11 biennial and 9 perennial weeds in uplands in Jeonbug Province. Digitaria sanguinalis Scopol. occurred with the highest frequency of 67.2% and in decreasing order Portulaca oleracea L., Chenopodium album L. and Erigeron canadensis L. followed. About 60% of total sites studied indicated Simpson`s index ranging from 0.20 to 0.50. The Simpson`s index decreased from 0.65 on June 8 to 0.15 on November 10. Dominant weed species were Alopecurus aequalis Sosbol, and Polygonum hydropiper Spach in double cropping paddy field, but D. sanguinalis and P. oleracea were the dominant species in uplands. In reclaimed uplands various weed species including, 4rtemisia asiatica Nakai occurred almost equally. Average Simpson`s index for six crops studied was 0.40 and irrespective of the crops being cultivated P. oleracea and D. sanguinalis were dominant weed species and Y. hydropiper, C. album, Exolus blitum Grenier and E. canadensis were sub-dominant.

• Investigative Magnetic Resonance Imaging
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• v.15 no.1
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• pp.41-47
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• 2011

Purpose : To evaluate the cause of internal jugular vein (IJV) obstruction on contrast enhanced 3D MR angiography (CE-MRA) using contrast enhanced computed tomography (CE-CT). Materials and Methods : A total number of 30 patients were enrolled, who underwent both head and neck CE-MRA and CE-CT from 2005 to 2008. We defined obstruction group which had IJV obstruction and control group which had no IJV obstruction on CE-MRA. The following parameters were measured from axial images of CE-CT: 1) diameter of IJV; 2) distance between the styloid process and ipsilateral lateral mass of the atlas; 3) maximum area of lateral mass of the atlas. Each parameter was compared between obstruction group and control group. Results : The diameter of IJV and distance between the styloid process and lateral mass of the atlas at IJV obstruction side in obstruction group were $1.6{\pm}1.0\;mm$ and $4.1{\pm}2.1\;mm$ respectively, which resulted in statistical significance (p<0.01). The maximum area of lateral mass of the atlas at IJV obstruction side in obstruction group was $103.4{\pm}25.3\;mm^2$ which is significantly larger than in control group (p<0.05). Conclusion : We found that the cause of IJV obstruction on CE-MRA could be narrow space between the styloid process and the lateral mass of the atlas, which was related with asymmetric larger area of lateral mass of atlas.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.2
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• pp.768-777
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• 2013

The purpose of this study is to be conducive to work improvement through analyzing turnover intention and organizational commitment of EMT within medical institution. The subjects of this study were randomly extracted 188 EMT. The survey period was conducted from May 3, 2011 to May 19 (May 3rd-4th in G Province, May 14th in B Metropolitan City, May 18th-19th in D Metropolitan City). An analytical method was made with SPSS WIN program(Version 12.0). As a result of research, the turnover intention was high in women(p<.001), in under 30 years old(about 4.9 times, p<.001), in the unmarried(about 4.3 times, p<.001), and in junior college graduate(about 4 times, p<.001). Also, the turnover intention was high in income with under 1,500,000 won(about 3.3 times, p<.001), in working career with under 5 years(about 3.2 times, p<.001), in feeling the legal work limit(about 1.8 times, p<.05), and in group with low job satisfaction(p<.001). In correlation between organizational commitment and job variable, the variables in age, marriage, academic background, turnover intention, income, career, and legal work limit except gender and job satisfaction showed positive correlation with organizational commitment(p<.01). As for the factors affected on the organizational commitment, the turnover intention(p<.01), the legal work limit(p<.01), and the job satisfaction(p<.001) had significant effect on organizational commitment.

• Korean Journal of Remote Sensing
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• v.36 no.2_1
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• pp.199-215
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• 2020

A seagrass that is marine vascular plants plays an important role in the marine ecosystem, so periodic monitoring ofseagrass habitatsis being performed. Recently, the use of dronesthat can easily acquire very high-resolution imagery is increasing to efficiently monitor seagrass habitats. And deep learning based on a convolutional neural network has shown excellent performance in semantic segmentation. So, studies applied to deep learning models have been actively conducted in remote sensing. However, the segmentation accuracy was different due to the hyperparameter, various deep learning models and imagery. And the normalization of the image and the tile and batch size are also not standardized. So,seagrass habitats were segmented from drone-borne imagery using a deep learning that shows excellent performance in this study. And it compared and analyzed the results focused on normalization and tile size. For comparison of the results according to the normalization, tile and batch size, a grayscale image and grayscale imagery converted to Z-score and Min-Max normalization methods were used. And the tile size isincreased at a specific interval while the batch size is allowed the memory size to be used as much as possible. As a result, IoU was 0.26 ~ 0.4 higher than that of Z-score normalized imagery than other imagery. Also, it wasfound that the difference to 0.09 depending on the tile and batch size. The results were different according to the normalization, tile and batch. Therefore, this experiment found that these factors should have a suitable decision process.

• BMB Reports
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• v.39 no.4
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• pp.432-440
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• 2006

A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. The purified A. jacquemontii lectin (AJL) showed a single protein band with an apparent molecular mass of 13.4 kDa when submitted to SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The native molecular mass of AJL determined by gel filtration on a Biogel P-200 column was 52 kDa and its carbohydrate content was estimated to be 3.40%. Thus AJL is a tetrameric glycoprotein. The purified lectin agglutinated erythrocytes from rabbit but not from human. Its activity was not inhibited by any of the mono- and disaccharides tested except N-acetyl-D-lactosamine having minimal inhibitory sugar concentration (MIC) 25 mM. Among the glycoproteins tested only asialofetuin was found to be inhibitory (MIC $125\;{\mu}g/mL$). A single band was obtained in native PAGE at pH 4.5 while PAGE at pH 8.3 showed two bands. Isoelectric focusing of AJL gave multiple bands in the pI range of 4.6-5.5. When incorporated in artificial diet AJL significantly affected the development of Bactrocera cucurbitae (Coquillett) larvae indicating the possibility of using this lectin in a biotechnological strategy for insect management of cucurbits. Larvae fed on artificial diet containing sub-lethal dose of AJL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. Out of various human cancer cell lines employed in sulphorhodamine B (SRB) assay, this lectin was found to have appreciable inhibitory effect on the in vitro proliferation of HCT-15, HOP-62, SW-620, HT-29, IMR-32, SKOV-3, Colo-205, PC-3, HEP-2 and A-549 cancer cell lines by 82, 77, 73, 70, 41, 41, 37, 29, 21 and 21% respectively.

• Economic and Environmental Geology
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• v.47 no.3
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• pp.303-316
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• 2014

This study was carried out to raise research productivity through the analysis on the research projects and to find trends of mineral resources survey and mineral deposits of the KIGAM for 38 years since the fiscal year of 1976 to 2013. The KIGAM in the projects for mineral resources survey and mineral deposits expended 67.41 billion won for last 38 years, which is 107.07 billion won calculated using the present value in 2013 at discount rate of 5%. R&D expenditure in mineral resources survey and mineral deposits of KIGAM increased approximately 59.4 times from 108 million won in 1976 to 6,420 million won in 2013, and about 9.8 times from 657 million won in 1976, as calculated using the present value in 2013. The percentage of research projects in mineral resources survey by research sub-areas was as follows: survey, 32%(90 projects); origin of ore deposits and basic research, 19%(54 projects); exploration for ore deposits, 17%(48 projects); database and information, 13%(37 projects); mineralogy and ore analysis, 9%(25 projects); and etc., 10%(27 projects). Based on analysis of research trends over the last 38 years, to raise research productivity, the existing survey research techniques with repeated use and related technology development should be enhanced.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.470-478
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• 2016

A bacterium producing a large amount of chitinolytic enzyme was isolated from the intestinal tract of earthworm. The isolate was identified as Bacillus licheniformis by 16S ribosomal RNA analysis and designated as B. licheniformis GA9. The enzyme was purified by 40-60% ammonium sulfate precipitation, diethyl-aminoethyl groups exchange chromatography, and gel filtration chromatography. The molecular weight was estimated to be 52.1 kDa and the N-terminal amino acid sequence was D-S-G-K-N-G-K-I-I-R-Y-YP-I-R. The optimum activity of the purified chitinolytic enzyme was shown at pH 5.0 and $40^{\circ}C$, and the enzyme was stable in the ranges of $20-50^{\circ}C$ and pH 5.0-6.0. Enzyme activity was increased by $Co^{2+}$, while it was inhibited by $Cu^{2+}$ and $Fe^{2+}$. But it was recovered by chelating metals with ethylenediaminetetraacetic acid. The $K_m$ and $V_{max}$ values of the purified enzyme were 4.02 mg/ml and 0.52 mg/min, respectively. The chitinolytic enzyme characterized in this study has potential applications in areas such as biotechnology, biomedicine, agriculture, and nutrition.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.49-54
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• 2017

In the course of screening tyrosinase inhibitory activity, EtOAc-soluble fraction of Coix lachryma-jobi var. mayuen Stapf. (Gramineae) roots showed significant inhibition. Further fractionation of the EtOAc-soluble fraction resulted in six compounds, which were identified as (+)-icariol $A_2$ (1), zhepiresionol (2), 4-hydroxybenzaldehyde (3), trans-${\rho}$-coumaric acid (4), N-(2-hydroxy-4-methoxyphenyl)-2-hydroxyacetamide (5), and coixol (6). The chemical structures of these compounds were identified on the basis of spectroscopic methods (MS, 1D and 2D NMR) and comparison with literature values. Compound 1 was first reported from this plant. Also, this is the first time that the isolation of compound 5 has been reported from nature source. Among the isolated compounds, compounds 4 and 6 showed enzyme inhibitory activity, with $IC_{50}$ values of 6.5 and $62.4{\mu}M$, respectively, in comparison with these of positive control, arbutin.

• Journal of Broadcast Engineering
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• v.10 no.4 s.29
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• pp.474-487
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• 2005

T-DMB (Terrestrial-Digital Multimedia Broadcasting) adopted MPEG-4 BIFS (Binary Format for Scene) Core2D scene description profile and graphics profile as the standard of video associated data service. By using BIFS, we can support to overlay objects, i.e. text, stationary image, circle, polygon, etc., on the main display of receiving end according to the properties designated in broadcasting side and to make clickable buttons and website links on desired objects. Therefore, a variety of interactive data services can be served by BIFS. In this paper, we implement real-time video associated data service system far T-DMB. Our developing system places emphasis on real-time data service by user operation and on inter-working and stability with our previously developed video encoder. Our system consists of BIFS Real-time System, Automatic Stream Control System and Receiving Monitoring System. Basic functions of our system are designed to reflect T-DMB programs and characteristics of program production environment as a top priority. Our developed system was used in BIFS trial service via KBS T-DMB, it is supposed to be used in T-DMB main service after improvement process such as intensifying system stability.