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General Activity and Stress Response of Rats Following Removal of the Mamillary Bodies (유두체를 떼어버린 흰쥐의 일반활동 및 스트레스에 대한 반응)

  • Kim, Chul;Choi, Hyun;Kim, Chung-Chin;Kim, Jong-Kyu;Kim, Myung-Suk
    • The Korean Journal of Physiology
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    • v.2 no.1
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    • pp.79-88
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    • 1968
  • For the purpose of assessing the influence of the mamillary bodies on the general activity and stress response, mamillary body·damaged rats(mamillary body group), operated control rats, and normal control animals were prepared and 2 experiments were carried out. The mamillary bodies. were damaged electrolytically by passing 0.3 ma d.c. current through stereotaxically implanted electrodes. The operated control group received the same treatment except passage of current. In the 1st experiment, each subject belonging to the mamillary body group .(9 rats) or the operated control group (13 animals) was put individually into an activity cage across which an infrared beam traversed. The number of beam interruptions by the movement of the subject in the cage was. recorded hourly for 48 hours and was regarded as an index of general activity. In the 2nd experiment, each of the mamillary body group (52 animals), the operated control group (45 rats), and the normal control group (37 animals) was further divided into 4 subgroups and the adrenal ascorbic acid content of one of the 4 subgroups was measured before, another subgroup immediately after, a third subgroup 2 hours after, and the remaining subgroup 4 hours after termination of a cold stress ($-10^{\circ}C$ for 1 hour). Following results were obtained: 1. Though the total activities in 48 hours of the mamillary body group and the operated control group were not significantly different, the activities of the malnillary body group for a few hours. at the beginning of the measurement and also in the evenings of both the 1st and the 2nd experimental days were significantly greater than those of the operated control group. 2. Judged by the significant reduction in adrenal ascorbic acid content, the stress response of the mamillary body group was as marked as that of the operated control or the normal control group-immediately after termination of cold exposure. Recovery from the stress was accelerated significantly in the mamillary body group and insignificantly in the operated control group compared with that of the normal control group, the intergroup difference of the former two groups being insignificant. It was inferred from the above results that the mamillary bodies may exert an inconspicuous inhibitory influence upon the central mechanism of general activity, and that the mamillaryies bodies may not be the main seat of the stress mechanism, although more works are desirable to confirm the results.

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TEC VARIATIONS OVER KOREAN PENINSULA DURING MAGNETIC STORM (남쪽방향 행성간 자기장에 의해 발생한 자기 폭풍 동안 한반도 상공의 총 전자수 함유량 변화)

  • Ji, E.Y.;Choi, B.K.;Kim, K.H.;Lee, D.H.;Cho, J.H.;Chung, J.K.;Park, J.U.
    • Journal of Astronomy and Space Sciences
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    • v.25 no.1
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    • pp.33-42
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    • 2008
  • By analyzing the observations from a number of ground- and space-based instruments, including ionosonde, magnetometers, and ACE interplanetary data, we examine the response of the ionospheric TEC over Korea during 2003 magnetic storms. We found that the variation of vertical TEC is correlated with the southward turning of the interplanetary magnetic field $B_z$. It is suggested that the electric fields produced by the dynamo process in the high-latitude region and the prompt penetration in the low- latitude region are responsible for TEC increases. During the June 16 event, dayside TEC values increase more than 15%. And the ionospheric F2-layer peak height (hmF2) was ${\sim}300km$ higher and the vertical $E{\times}B$ drift (estimated from ground-based magnetometer equatorial electrojet delta H) showed downward drift, which may be due to the ionospheric disturbance dynamo electric field produced by the large amount of energy dissipation into high-latitude regions. In contrast, during November 20 event, the nightside TEC increases may be due to the prompt penetration westward electric field. The ionospheric F2-layer peak height was below 200km and the vertical $E{\times}B$ drift showed downward drift. Also, a strong correlation is observed between enhanced vertical TEC and enhaaced interplanetary electric field. It is shown that, even though TEC increases are caused by the different processes, the electric field disturbances in the ionosphere play an important role in the variation of TEC over Korea.

Study of Selective Etching of GaAs over AlGaAs and InGaP Semiconductors in High Density Planar Inductively Coupled BCl3/SF6 Plasmas (고밀도 평판형 유도결합 BCl3/SF6 플라즈마를 이용한 GaAs/AlGaAs와 InGaP 반도체의 선택적 식각에 관한 연구)

  • Yoo Seungryul;Ryu Hyunwoo;Lim Wantae;Lee Jewon;Cho Guan Sik;Jeon Minhyon;Song Hanjung;Lee BongJu;Ko Jong Soo;Go Jeung Sang;Pearton S. J.
    • Korean Journal of Materials Research
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    • v.15 no.3
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    • pp.161-165
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    • 2005
  • We investigated selective dry etching of GaAs over AlGaAs and InGaP in high density planar inductively coupled $BCl_3/SF_6$ plasmas. The process parameters were ICP source power (0-500 W), RE chuck power (0-30W) and gas composition $(60-100\%\;BCl_3\;in\;BCl_3/SF_6)$. The process results were characterized in terms of etch rate, selectivities of GaAs over AlGaAs and InGaP, surface morphology, surface roughness and residues after etching. $BCl_3/SF_6$ selective etching of GaAs showed quite good results in this study. Selectivities of GaAs $(GaAs:AlGaAs\~36:1,\;GaAs:InGaP\~45:1)$ were superior at $18BCl_3/2SF_6$, 20 W RF chuck power, 300 W ICP source power and 7.5 mTorr. Addition of $(5-15\%)SF_6\;to\;BCl_3$ produced relatively high selectivities of GaAs over AlGaAs and InGaP during etching due to decrease of etch rates of AlGaAs and InGaP (boiling points of etch products: $AlF_3\~1300^{\circ}C,\;InF_3>1200^{\circ}C$ at atmosphere) at the condition. SEM and AFM data showed slightly sloped sidewall and somewhat rough surface$(RMS\~9nm)$. XPS study on the surface of processed GaAs proved a very clean surface after dry etching. It shows that planar inductively coupled $BCl_3/SF_6$ plasmas could be a good candidate for selective dry etching of GaAs over AlGaAs and InGaP.

Herbicidal Activities of Dinitroaniline Compounds in Turfgrass (Dinitroaniline계(系) 제초제(除草劑)의 잔디밭에서의 작용특성(作用特性))

  • Lee, Y.D.;Kim, S.J.;Kim, K.W.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.265-271
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    • 1994
  • This study was conducted to determine the herbicidal activity, persistance, downward movement and effect on bentgrass of 7 dinitroaniline herbicides such as benefin [N-butyl-N-ethyl-2,6-dinitro-4-(trifluoromethyl) benzenamine], beslogine [N,N-dibutyl-2,6-dinitro-4-trifluoromethylaniline], prodiamine [2,4-dinitro-$N^3$,$N^3$-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine], pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine], trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl) benzenamine], ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine], and oryzalin [4-(diprop-ylamino)-3,5-dinitrobenzene-sulfonamide) together with bensulide [O,O-bis(1-methylethyl) S-[2-[(phenyl-sulfonyl) amino]ethyl]phosphorodithioate] and siduron [N-(2-methylcyclohexyl)-N'-phenylurea) as the control. In addition, distribution of pendimethalin and benefin into bentgrass was also determined. Prodiamine, benefin, and pendimethalin at the 1/16 dose of the recommended rate showed very high herbicidal activity($LD_{90}$) on Digitaria sanguinalis, but ethalfluralin and bensulide showed $LD_{90}$ at the 1/4 dose of the recommended rate, showing difference in herbicidal activities among dinitroaniline herbicides. All the herbicides except for pendimethalin had the lower herbicidal activity in sandy soil than that of clay-loam soil. Benefin, beslogine, prodiamine, oryzalin, bensulide and siduron persisted in the soil for about 50 days, but pendimethalin persisted in the soil for about 35 days, and trifluralin and ethalfluralin for about 25 days. Ethalfluralin, oryzalin and bensulide were the most mobile(downward movement) of the 9 herbicides studied. Less mobility was observed in the turfgrass condition than that of the bare soil. Beslogine bensulide prodiamine and benefin had no injury effect on bentgrass(Agrastis atolonifera L., penncross creeping bent grass). However, herbicides like oryzalin, trifluralin and pendimethalin reduced the dry weight of bentgrass by 12%, 30% and 40%, respectively. No significant difference in distribution of pendimethalin and benefin into inner part of leaves, surface and wax layer of bentgrass was observed, and thus it seems that different phytotoxic effect between pendimethalin and benefin may be attributed to different metabolism and mode of action.

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Weed Flora Changes in Lowland Rice Field in Gangweon Province (강원지역(江原地域) 지대별(地帶別) 논잡초(雜草) 분포(分布))

  • Kim, K.S.;Kim, J.R.;Ko, J.H.;Sa, J.G.;Chang, J.S.;Kim, D.Y.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.258-264
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    • 1994
  • The weed survey in lowland rice field in 1992 was conducted to identify weed population change as affected by different elevation, soil type and cultivation pattern at Gangweon province. There were more perennial weeds in plain and east coastal area while more annual weeds in mid alpine and alpine area. Meanwhile, perennial weed species was more dominant at ordinary and sandy soil but annual weed species was more dominant at poorly drained soil. In general, hand and machine(30 day old seedling) transplanting would result in higher dominant weed species of perennial while there was more annual weed species in machine (10 day old seedling) transplanting and dry seeding, respectively. Also, annual weed species was more predominant at spring plowing time compared to autumn plowing time. Particularly, there was change in dominant weed species with time. In 1971 annual weed species was more predominant but perennial weed species was highly dominant in 1981 and 1992.

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Study on the Effects of an Organic Fertilizer (Glutamic Acid Fermentation Residue Amended with N) on the Yield of Chinese Cabbage and Radish and the Physico-Chemical Properties of Soil (유기질비료(有機質肥料) 시용(施用)이 배추와 무우의 생육(生育)과 수량(收量) 및 토양(土壤)의 이화학성(理化學性)에 미치는 영향(影響))

  • Lim, S.U.;Ryu, J.C.;Hong, C.W.
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.3
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    • pp.125-132
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    • 1979
  • A field experiment was conducted to evaluate the effectiveness of an organic fertilizer (Glutamic acid fermentation by product ameded with nitrogen) on the growth and yield of radish and chinese cabbage on a relatively fertile soil. Additional laboratory studies were also made to find out the influence of mentioned fertilizer on the phisical and chemical characteristic of soils. The results are summarized as following. 1. Effect of fertilizer on the growth and yield. a. On a relatively fertile soil with high organic matter content (3%), the organic fertilizer was not effective for the growth and yield of radish and yield of radish and chinese cabbage. b. The application of organic fertilizer increased the K contents in plants both in radish and chinese cabbage. It was observed that the high content of K in radish leave lowered the leaf/root ratio and the leaf/root ratio showed a positive correlation with root weight. 2. Effect on soil physical and chemical properties. a. The application of organic fertilizer resulted in the improvement of soil physical property by lowering bulk density and increasing porosity. b. It was found that application of organic fertilizer increased the water stable soil aggregates. c. The application of organic fertilizer did not result in the significant increase in soil organic matter. d. Soil pH was lowered by the application of organic matter. It was speculated that the oxidation of ammonical nitrogen in the fertilizer is responsible for the lowering of soil pH.

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A STUDY FOR OCCLUSAL FEATURES OF FIRST PERMANENT MOLAR AND SECOND PRIMARY MOLAR (제 1대구치와 제 2유구치의 교합면 양상에 관한 연구)

  • Jeon, So-Hee;Kim, Jae-Gon;Yang, Yeon-Mi;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.1
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    • pp.89-100
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    • 2005
  • The purpose of this study was to analyze the morphometrics of primary second molar and permanent first molar. Samples were consisted of normal occlusion in the primary dentition(50 males and 50 females) and permanent dentition(43 males and 43 females). Their upper and lower plaster casts were used and their measuring points were decided, through 3-dimensional laser scanning(3D Scanner, DS4060, LDI, U.S.A.), fitting standard horizontal plane were made for measuring the intercuspal distance, volume of intercuspal area and section curve. The results were as follows; 1. Average distance from the fit plane to the cusp tips of mandibular primary second molar was smaller than any other tooth. (0.05-0.09 mm in male and 0.04-0.09 mm in female). 2. Intercuspal distances of mandibular primary second molar and permanent first molar were larger in male than in female. Especially, there was statistical significance in primary second molar(p<0.05). 3. Intercuspal distance between distobuccal and distolingual cusp was larger in maxillary primary second molar, except cross intercuspal distances. And distances between distal and distolingual cusp, in mandibular primary second molar, between mesiolingual and mesiobuccal cusp, in maxillary first molar, and between distolingual and mesiolingual cusp, in mandibular first molar were larger than any other intercuspal distance. 4. Volume of intercuspal area of primary second molar and permanent first molar was larger in mandible than in maxilla and that of permanent first molar was 1.40-1.75 times of primary second molar (p<0.05). Also it was larger in male than in female, but there was no statistical significance. 5. In most cases, section curves were wider and deeper in permanent dentition than in primary dentition. Except cross intercuspal distances, in maxilla, section curve between mesiobuccal and mesiolingual cusp was the deepest in both dentition. In mandible, section curve between distobuccal and distal cusp was the deepest in permanent dentition and between distolingual and distal cusp was the deepest in primary dentition.

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Inhibition of Type II Diabetes in ob/ob Mice and Enhancement of Mitochodrial Biogenesis in C2C12 Myotubes by Korean Mistletoe Extract (한국산 겨우살이 추출물(KME)의 2형 당뇨 억제 및 근육세포 미토콘드리아 생성 증가 효과)

  • Jung, Hoe-Yune;Yoo, Yung Choon;Kim, Inbo;Sung, Nak Yun;Choi, Ok-Byung;Choi, Bo-Hwa;Kim, Jong-Bae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.3
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    • pp.324-330
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    • 2015
  • In this study, the anti-diabetic activity of a cold water extract of Korean mistletoe (KME) was investigated in C57BL/6J Lep ob (ob/ob) mice. Oral administration of KME (50 or 100 mg/kg/d) significantly inhibited the level of blood glucose of ob/ob mice after 5 days from the beginning of KME treatment. And the anti-diabetic effect of KME was stabilized 10 days after oral administration, showing a substantial reduction of blood glucose levels by more than 20% as compared with control mice. The results of oral glucose tolerance test (OGTT) revealed that oral administration of KME gave rise to a remarkable improvement in overall glucose response. Oral administration of KME in ob/ob diabetic mice also significantly reduced blood total cholesterol (TCHO) and triglyceride (TG) levels compared with the diabetic control mice. Moreover, in an in vitro experiment using C2C12 myotubes, treatment of KME prominently increased glucose uptake. Interestingly, KME significantly increased the expression of peroxisome proliferator-activated receptor gamma coactivator 1-${\alpha}$ ($PGC-1{\alpha}$), a head regulator of mitochondrial biogenesis and oxidative metabolism, and $PGC-1{\alpha}$-associated genes such as glucose transporter type 4 (GLUT4), estrogen-related receptor-${\alpha}$ ($ERR-{\alpha}$), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TmfA) in C2C12 cells. These results suggest that KME has potential as a novel therapeutic agent for diabetes, and its anti-diabetic activity may be related to the regulation of mitochondrial biogenesis.

A Study of Industrial Workers' Knowledge, Attitude, and Practice of Health Care Services in Busan City (산업장 근로자의 산업보건관리에 대한 지식 태도 및 실천에 관한 조사 연구 -부산지역을 중심으로-)

  • Huang, Bo-Sun;Shin, Yu-Sun;Yun, Suk-Ok;Lee, Ji-Hyun;Kim, Jung-Soon;Kim, Lee-Soon;Kim, Bok-Yong;Kang, Young-Mee
    • Research in Community and Public Health Nursing
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    • v.4 no.2
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    • pp.100-108
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    • 1993
  • The purpose of this study was to identify the workers the Knowledge, Attitude, and Practice of industrial health care services. The study was conducted from Dec 10, 1992 to Jan 20, 1993. The data was collected from 849 workers in 56 companies in Busan City. The data was analyzed by using the mean, S.D>, T-test, ANOVA. The tool for measuring the degree of Knowledge, Attitude and Practice of industrial health care of workers was developed by community health nursing academy and the reliability an of the tool was 0.82810. The results were as follows : 1. The general characteristics of workers : Distribution of workers consisted of laborer(55.0%), office workers(45.0%), in an age range from 25-39(55.0%), male employees were(69.7%), married employees were(62.4%), the educational level with the highest percentage was high school graduates(54.2%). The present work force had a career for 7 years or more with an income of 300-700 thousand won monthly(43.2). 2. The degree of Knowledge Attitude and Practice about industrial health care services of workers : The total score of the Knowledge, Attitude, and Practice was 49.09 points out of a possible 80(mean Score=2.46) 1) The total score of the Knowledge was 15.73 points out of 24. (Mean score=2.62) The following are the Knowledge scores: The necessity examination of occupational disease was(3.34) The knowledge of occupational disease was (3.12) The knowledge of health education was(1.29) 2) b. The total score of the Attitude was 26.01 point out of 44(mean score=2.36) The following are the Attitude scores: The necessity of health education for health examination was (3.14). The importance of health examination was(3.08) The necessity of measurement for working environment was(2.99). The satisfaction of the content in the periodic health examination was low(1.81). 3) The total score of the Practice was 7.35 points out of 12(mean score=2.45) The following are the orders of Practice scores: The participation in health examination was higher than in health education(1.33). 3. The general characteristics and the degree of knowledge, Attitude, and Practice of workers of industrial health care. 1) The level of worker's knowledge was significantly related by working part(t=2.54 P=0.000), marital status(F=4.35 P=0.029), educational level(F=3.91 and P=0.020), monthly income(F=2.98 P=0.029) 2) There were no significant difference between the general characteristics and the attitude of workers. 3) The practice was significantly related with working part(T=2.52 P=0.012), sex(T=2.28 P=0.23), marital status(F=4.25 P=0.012), monthly income (F=2.76 P=0.034) 4) The total score had a significant difference with working part(T=2.39 P=0.017), sex (T=3.84 P=0.000), marital status (F=3.18 P=0.032). Educational level (F=3.20 P=0.033), and monthly income(F=3.05 P=0.022).

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Expression Pattern of Skeletal-Muscle Protein Genes and Cloning of Parvalbumin mRNA in Dark-banded Rockfish (Sebastes inermis) (볼락(Sebastes inermis) 근육단백질 유전자의 성장단계별 발현 양상과 parvalbumin 유전자 클로닝)

  • Jang, Yo-Soon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.1-9
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    • 2011
  • Differentially Expressed Gene (DEG) was obtained from Differential Display Reverse Transcription (DDRT)-PCR using Annealing Control Primer (ACP) to search and clone genes related to developmental stages of Sebastes inermis. By using 120 ACPs, the nucleotide sequences obtained from 16 DEGs showing higher expression in 6-month-old skeletal muscle than 18-month-old ones and from 22 DEGs displaying stronger expression in 18-month-old than 6-month-old were analyzed and BLAST was conducted. The results identified that DEGs shared 69~95% homology with genes of parvalbumin (PVALB), nucleoside diphosphate kinase (NDK) B, tropomyosin (TPM), troponin I (TnI), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), muscle-type creatine kinase (CKM2), small EDRK-rich factor 2 (SERF2), adenosine monophosphate deaminase (AMPD), Trimeric intracellular cation channel type A (TRICA), Rho GTPase-activating protein 15 (ARHGAP15), S-formylglutathione hydrolase (Esterase D; ESD), heat shock protein 70 (hsp70), type 1 collagen alpha 2 (COL1A2), glutathione S-transferase, Mid1-interacting protein 1 (Mid1lip1), myosin light chain 1 (MYL1), sarcoplasmic/endoplasmic reticulum calcium ATPase 1B (SERCA1B), and ferritin heavy subunit (FTH1). Expression pattern by developmental stage of DEG14 and PVALB exhibiting strong expression in 6-month-old skeletal muscle was investigated using real time PCR. Expression was reduced as Sebastes inermis grew. Expression of PVALB gene was extremely low after 6 months of age. Expression of CKM2 showed higher expression in 18-month-old skeletal muscle than in 6-month-old muscles, and increased continuously until 4 years old, after which CKM2 expression became gradually reduced. By analysis of tissue-specific expression patterns of DEG, DEG14 was expressed mainly in skeletal muscle, liver, kidney and spleen tissues, whereas PVALB expression was expressed in skeletal muscle and kidney, but not in liver and spleen tissues. CKM2 was expressed in skeletal muscle, kidney, and spleen tissues, but not in liver tissues. PVALB gene was composed of 110 amino acids, which constituted 659 bp nucleotides. The results reported here demonstrate that the expression patterns of parvalbumin and CKM2 could be used as molecular markers for selecting fishes exhibiting fast growth.