• 제목/요약/키워드: D/N value

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Asn124 of Cel5A from Hypocrea jecorina not only provides the N-glycosylation site but is also essential in maintaining enzymatic activity

  • Qin, Yuqi;Qu, Yinbo
    • BMB Reports
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    • 제47권5호
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    • pp.256-261
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    • 2014
  • To investigate the function of N-glycosylation of Cel5A (endoglucanase II) from Hypocrea jecorina, two N-glycosylation site deletion Cel5A mutants (rN124D and rN124H) were expressed in Saccharomyces cerevisiae. The weights of these recombinant mutants were 54 kDa, which were lower than that of rCel5A. This result was expected to be attributed to deglycosylation. The enzyme activity of rN124H was greatly reduced to 60.6% compared with rCel5A, whereas rN124D showed slightly lower activity (10%) than that of rCel5A. rN124D and rN124H showed different thermal stabilities compared with the glycosylated rCel5A, especially at lower pH value. Thermal stabilities were reduced and improved for rN124D and rN124H, respectively. Circular dichroism spectroscopy showed that the modification of secondary structure by mutation may be the reason for the change in enzymatic activity and thermal stability.

DIFFERENTIAL EQUATIONS ON CLOSED SUBSETS OF A PROBABILISTIC NORMED SPACE

  • Kim, Jong-Kyu;Jin, Byoung-Jae
    • Journal of applied mathematics & informatics
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    • 제5권1호
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    • pp.223-233
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    • 1998
  • This paper is concerned with the problem of existence of solutions to the initial value problem u'(t) = A(t, u(t)), u(a) = z in a probabilistic normed space where $A : [a,b)\;{\times}\;D->E$ is continuous, D is a closed subset of a probabilistic normed space E, and $z\;{\in}\;D$. With a dissipative type condition on A, we estabilish sufficient conditions for this initial value problem to have a solution.

Viscosity and Diffusion Constants Calculation of n-Alkanes by Molecular Dynamics Simulations

  • Lee, Song-Hi;Chang, Tai-Hyun
    • Bulletin of the Korean Chemical Society
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    • 제24권11호
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    • pp.1590-1598
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    • 2003
  • In this paper we have presented the results for viscosity and self-diffusion constants of model systems for four liquid n-alkanes ($C_{12}, C_{20}, C_{32}, and C_{44}$) in a canonical ensemble at several temperatures using molecular dynamics (MD) simulations. The small chains of these n-alkanes are clearly $<{R_{ee}}^2>/6<{R_g}^2>>1$, which leads to the conclusion that the liquid n-alkanes over the whole temperatures considered are far away from the Rouse regime. Calculated viscosity ${\eta}$ and self-diffusion constants D are comparable with experimental results and the temperature dependence of both ${\eta}$ and D is suitably described by the Arrhenius plot. The behavior of both activation energies, $E_{\eta}$ and $E_D$, with increasing chain length indicates that the activation energies approach asymptotic values as n increases to the higher value, which is experimentally observed. Two calculated monomeric friction constants ${\zeta}$ and ${\zeta}_D$ give a correct qualitative trend: decrease with increasing temperature and increase with increasing chain length n. Comparison of the time auto-correlation functions of the end-to-end vector calculated from the Rouse model for n-dodecane ($C_{12}$) at 273 K and for n-tetratetracontane ($C_{44}$) at 473 K with those extracted directly from our MD simulations confirms that the short chain n-alkanes considered in this study are far away from the Rouse regime.

NOTE ON THE MODULUS METHOD IN Rn

  • Chung, Bo-Hyun
    • 충청수학회지
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    • 제16권2호
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    • pp.23-30
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    • 2003
  • In this note, we introduce the concept of the modulus of a curve family in $R^n$ and examine some basic properties. And we study the boundary behavior of quasiconformal mappings on a domain $D{\subset}\bar{R}^n$ and present some geometric applications.

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Al-MIS(p-Si) 태양전지의 AR Coating 방법 (On the AR Coating Method of Al-MIS(p-Si) Solar Cel)

  • 엄경숙;백수현
    • 대한전자공학회논문지
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    • 제21권6호
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    • pp.64-69
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    • 1984
  • Al-MIS(p-Si)태양전지의 최대 효율은 Al 박막을 0.6A/sec 이하의 속도로 증착시켜 80Å인 두께가 되었을 때임을 알았다. ZnS와 SiO로 AR coating을 했는데, 단층 피막일때는 각각 570Å과 690Å인 박막 두께에서 최대 Isc를 나타냈다. 이는 quarter-coating 조건인 n1d1=λ/4를 만족함을 알았다. 이중층 피막일 때는 한 층을 단층 피막때의 최적 두께를 유지하면서 다른 한 층을 조절했다. 이때의 최대 Isc는 단층피막일 때와 비슷한 값이었으나 넓은 범위의 두께에서 그 값이 그대로 유지되었다. 한편 dzns/ds10=1.9/2.3=570/690인 관계를 유지하면서 총 두께를 각각의 단층피막때의 최적 두께 합에 대해서 70∼90%로 얇게 했을 때 앞에서 논의한 어떤 경우보다도 20%이상의 더 높은 Isc를 얻을 수 있었다 따라서 높은 효율을 얻으면서 정밀한 두께 조절이 불필요한 새로운 AR coating방법을 얻었다.

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Characteristic study of bell-shaped anchor installed within cohesive soil

  • Das, Arya;Bera, Ashis Kumar
    • Geomechanics and Engineering
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    • 제27권5호
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    • pp.497-509
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    • 2021
  • A large deformation FEM (Finite Element Method) based numerical analysis has been performed to study the behaviour of the bell-shaped anchor embedded in undrained saturated (cohesive) soil with the help of finite element based software ABAQUS. A typical model anchor with bell-diameter of 0.125 m, embedded in undrained saturated soil with varying cohesive strength (from 5 kN/m2 to 200 kN/m2) has been chosen for studying the characteristic behaviour of the bell-shaped anchor installed in cohesive soil. Breakout factors have been evaluated for each case and verified with the results of experimental model tests for three different types of soil samples. The maximum value of breakout factor was found as about 8.5 within a range of critical embedment ratio of 2.5 to 3. An explicit model has been developed to estimate the breakout factor (Fc) for uplift capacity of bell-shaped anchor within clay mass in terms of H/D ratio (embedment ratio). It was also found that, the ultimate uplift capacity of the anchor increases with the increase of the value of cohesive strength of the soil and H/D ratio. The empirical equation developed in the present investigation is usable within the range of cohesion value and H/D ratio from 5 kN/m2 to 200 kN /m2 and 0.5 to 3.0 respectively. The proposed model has been validated against data obtained from a series of model tests carried out in the present investigation. From the stress-profile analysis of the soil mass surrounding the anchor, occurrence of stress concentration is found to be generated at the joint of anchor shaft and bell. It was also found that the vertical and horizontal stresses surrounding the anchor diminish at about a distance of 0.3 m and 0.15 m respectively.

디지털 제어 적분형의 차단 현상이 없는 A/D 다중 비트 $\Sigma\Delta$ 변조기 (A Clipping-free Multi-bit $\Sigma\Delta$ Modulator with Digital-controlled Analog Integrators)

  • 이동연;김원찬
    • 전자공학회논문지C
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    • 제34C권4호
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    • pp.26-35
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    • 1997
  • This paper proposes a multi-bit $\Sigma\Delta$ modulator arcitecture which eliminates signal clipping problem. To avoid signal clipping, the output values of intgrators are monitored and modified by a reference value. This oepration is recorded as a digital code to restore actual signal value. Due to the digital code, the substraction of feedback value from the multi-bit quantizer can be calculated by a digital adder and this simplifies dAC operation making the accurate DAC of conventional multi-bit $\Sigma\Delta$ modulator scheme unnecessary. These features make N-th modulator can be implemented by sharing an integrator among N stages to decrease the required chip area. As an experimental example, a 4th order .sum..DELTA. modulator with oversampling ratio of 64 was simulated to show over 130 DB SNR at rail-to-rail input sinusoidal signal.

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SOLUTIONS FOR A CLASS OF FRACTIONAL BOUNDARY VALUE PROBLEM WITH MIXED NONLINEARITIES

  • Zhang, Ziheng
    • 대한수학회보
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    • 제53권5호
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    • pp.1585-1596
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    • 2016
  • In this paper we investigate the existence of nontrivial solutions for the following fractional boundary value problem (FBVP) $$\{_tD_T^{\alpha}(_0D_t^{\alpha}u(t))={\nabla}W(t,u(t)),\;t{\in}[0,T],\\u(0)=u(T)=0,$$ where ${\alpha}{\in}(1/2,1)$, $u{\in}{\mathbb{R}}^n$, $W{\in}C^1([0,T]{\times}{\mathbb{R}}^n,{\mathbb{R}})$ and ${\nabla}W(t,u)$ is the gradient of W(t, u) at u. The novelty of this paper is that, when the nonlinearity W(t, u) involves a combination of superquadratic and subquadratic terms, under some suitable assumptions we show that (FBVP) possesses at least two nontrivial solutions. Recent results in the literature are generalized and significantly improved.

요소분해효소 억제물질의 작용과 응용에 관한 연구 (The Activity and Utilization of Urease Inhibitors)

  • 주영규
    • 아시안잔디학회지
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    • 제6권1호
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    • pp.23-28
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    • 1992
  • Urea, the major N source of world agriculture involves a serious urea-N loss through NH$_3$volatilization. Approaches to decrease N loss include using urease inhibitors in view of the environmental protection and the increase of urea-N efficiency. The purpose of laboratory researches was toassess the potential value of urease inhibitors to increase urea-N efficiency in soil and Kentucky blue-grass(Poa Pratensis L.) turf. The activity of urease inhibitors Phenyiphosphorodiamjdate(ppD) and N-(n-butyl) thiophosphoric triamjde(NBPT) measured to break-down ammonia volatilization. The soil and turf used in this project were from the fairway in one of the Korean gof course. The researches were carried out for two weeks to measure the urease activities on urea hydrolysis under four temperatures (10~ 40$^{\circ}C$) and for one week on turfgrass using forced-draft system. Results indicated that Urea-N involves considerable loss through gaseous NH$_3$ by urease activities in plant-soil systems. Urease inhibitors PPD and NBPT have potential value for increasing N use efficiency by reduing NH$_3$ volatilization. NBPT deserves futher evaluation as fertilizer amendment than PPD use of urea in turf industries.

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NUMERICAL IMPLEMENTATION OF THE QMR ALGORITHM BY USING DISCRETE STOCHASTIC ARITHMETIC

  • TOUTOUNIAN FAEZEH;KHOJASTEH SALKUYEH DAVOD;ASADI BAHRAM
    • Journal of applied mathematics & informatics
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    • 제17권1_2_3호
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    • pp.457-473
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    • 2005
  • In each step of the quasi-minimal residual (QMR) method which uses a look-ahead variant of the nonsymmetric Lanczos process to generate basis vectors for the Krylov subspaces induced by A, it is necessary to decide whether to construct the Lanczos vectors $v_{n+l}\;and\;w{n+l}$ as regular or inner vectors. For a regular step it is necessary that $D_k\;=\;W^{T}_{k}V_{k}$ is nonsingular. Therefore, in the floating-point arithmetic, the smallest singular value of matrix $D_k$, ${\sigma}_min(D_k)$, is computed and an inner step is performed if $\sigma_{min}(D_k)<{\epsilon}$, where $\epsilon$ is a suitably chosen tolerance. In practice it is absolutely impossible to choose correctly the value of the tolerance $\epsilon$. The subject of this paper is to show how discrete stochastic arithmetic remedies the problem of this tolerance, as well as the problem of the other tolerances which are needed in the other checks of the QMR method with the estimation of the accuracy of some intermediate results. Numerical examples are used to show the good numerical properties.