• Title/Summary/Keyword: D&M

Search Result 24,257, Processing Time 0.056 seconds

Effect of Group Spacing of Energy Piles on Thermal Analysis (말뚝 간격에 따른 에너지 파일의 열적 거동분석)

  • Min, Hye-Sun;Yun, Tae-Sup;Jeong, Sang-Seom
    • Journal of the Korean Geotechnical Society
    • /
    • v.27 no.8
    • /
    • pp.39-50
    • /
    • 2011
  • This study was conducted to analyze the thermal behavior of a PHC energy pi1e system in saturated soil conditions, various seasonal and flow-speed conditions during 100 hours of operation through numerical analysis. The examination was a1so conducted with a single pile as well as with group pils. For the operation of 100 hours, the average heat exchange rate appeared 55 W/m, 47 W/m during winter and summer respectively. An increase in flow-speed was associated with a rise in the heat exchange rate. And thermal behavior analysis results during winter season show that thermal efficiency has increased when there are more free thermal planes. For the operation in group pile as 3D and 5D pile spacing (D: pile diameter), average heat exchange rate increased as pile spacing grows. Compared with the heat exchange rate of single pile, thermal exchange efficiency of group pile decreased by 89% (for 3D spacing) and 93% (for 5D spacing).

TRIIODTHYRONINE (T3) ENHANCES THE STIMULATORY EFFECT OF 1, 25-DIHYDROXYVITAMIN D3 ON CALBINDIN-D28k mRNA EXPRESSION IN THE KIDNEY AND INTESTINE BUT NOT IN CEREBELLUM OF THE CHICK

  • Sechman, A.;Shimada, K.;Saito, N.;Ieda, T.;Ono, T.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.9 no.1
    • /
    • pp.37-44
    • /
    • 1996
  • The present study was conducted to investigate the role of thyroid hormones in the regulation of gene expression of calbindin-$D_{28k}$ (CaBP-D28K) in the chicken. By employing slot blot and RIA analyses, levels of CABP-D28K mRNA and CaBP-D28K protein in the intestine, kidney, cerebellum and liver were measured 6 and 12 h after i.m. injection of 1, 25-dihydroxyvitamin $D_3$ [1, 25 $(OH)_2D_3$; 250 ng/chick] and 3, 5, 3'-triiodothyronine ($T_3$; 500 ng/chick) in one-day-old chicks. The abundant messages of CaBP-D28K mRNA were detected in the intestine, kidney and cerebellum while there was little message in the liver. After 1, 25 $(OH)_2D_3$ treatment (6 + 12 hours), levels of CaBP-D28K mRNA increased in the intestine, but there was no change in the mRNA levels in the kidney and cerebellum. Although $T_3$ alone had no effect on CaBP-D28K mRNA levels, simultaneous administration of $T_3$ enhanced the 1, 25 $(OH)_2D_3$ effect of levels of CaBP-D28K mRNA in the intestine both 6 and 12 h post-treatment, and in the kidney 12 h post-treatment. At a protein level, co-treatment with 1, 25 $(OH)_2D_3$ and $T_3$ elicited a significant increase in CaBP-D28K expression in the intestine 12 h post-treatment, as compared to treatment with only 1, 25 $(OH)_2D_3$, whereas no differences were observed in the CaBP-D28K protein levels in the kidney and cerebellum. These results suggest that thyroid hormones may play a synergistic role with 1, 25 $(OH)_2D_3$ for CaBP-D28K gene expression in the intestine and kidney in chicks.

p-Fluorophenylalanine Resistant Cell Line Selection and Enzyme Activity from Diploid and Hapliod calli of Nicotiana tabacum cv. BY4 (담배 (Nicotiana tabacum cv. BY4)의 캘러스로부터 p-Fluorophenylalanine 저항성 캘러스 선발 및 효소활성도 측정)

  • 오승철;소웅영;조덕이;오승용;양덕춘
    • Korean Journal of Plant Tissue Culture
    • /
    • v.28 no.2
    • /
    • pp.69-74
    • /
    • 2001
  • Calli were induced on MS medium supplemented with 0.5 mg/L 2,4-D by using the leaf explants of haploid which were derived from the diploid and haploid of Nicotiana tabacum cv BY4. These calli were subcultured on MS medium with the combination of 2.0 mg/L 2,4-D, 1.0 mg/L kinetin and 0.1 mg/L BAP. Cell propagation of diploid plants were good in a combination of 2.0 mg/L 2,4-D, 0.1mg/L BAP in vitro conditions, suspension cultures were conducted in equal condition. Homogenized suspension cultured cells were smeared 2.0 mL each on MS medium with 0~100 $\mu$M PFP, to select the resistant colony to PFP, and were examined after 10d, 20d and 30d. Measurment of fresh weight of cells after 30d of culture shows that with more concentration of PFP in medium the fresh weight of the cells decreased. In case of diploid, selected callus was the highest in vitro treated with 5 $\mu$M PFP. It was higher than control until 100 $\mu$M PFP. The active degree of catalase was the highest in vitro with 5 $\mu$M PFP but the lowest in vitro with 10 $\mu$M PFP on the other hand, in case of haploid plant, the active degree of peroxidase and catalase was the highest in vitro treated with 50 $\mu$M PFP. It's sure that enzyme active degree of between diploid and haploid had big differences.

  • PDF

The Association of Metabolic Syndrome and Vitamin D in Korean Menopausal Women: Korea National Health and Nutrition Survey, 2010~2012 (한국 폐경기 여성에서 대사증후군과 비타민 D의 관련성: 2010~2012 국민건강영양조사에 근거하여)

  • Roh, Eun Kyung;Yoon, Hyun
    • Korean Journal of Clinical Laboratory Science
    • /
    • v.47 no.4
    • /
    • pp.318-323
    • /
    • 2015
  • The aim of this study was to assess the association of metabolic syndrome and Vitamin D in Korean adults. The study subjects were Korean menopausal woman (n=4,340) who participated in the Korea National Health and Nutrition Examination Survey 2010~2012. After adjusting for factors such as age, body mass index, total cholesterol, smoking, and regular exercise, the mean 25(OH)D levels ($M{\pm}SE$) decreased with increasing metabolic syndrome score (MSS) (MSS 0, $18.18{\pm}0.29ng/Ml$; MSS 1, $18.09{\pm}0.21ng/mL$; MSS 2, $18.07{\pm}0.19ng/mL$; MSS 3, $18.04{\pm}0.21ng/mL$; MSS ${\geq}4$, $17.27{\pm}0.23ng/mL$), and the mean 25(OH)D level ($M{\pm}SE$) for metabolic syndrome ($17.66{\pm}0.16ng/mL$) decreased in comparison to non-metabolic syndrome ($18.11{\pm}0.14ng/mL$). In conclusion, our results suggest that an increase in metabolic syndrome score or metabolic syndrome are inversely associated with the vitamin D levels.

Investigation of the Copper (Cu) Binding Site on the Amyloid beta 1-16 (Aβ16) Monomer and Dimer Using Collision-induced Dissociation with Electrospray Ionization Tandem Mass Spectrometry

  • Ji Won Jang;Jin Yeong Lim;Seo Yeon Kim;Jin Se Kim;Ho-Tae Kim
    • Mass Spectrometry Letters
    • /
    • v.14 no.4
    • /
    • pp.153-159
    • /
    • 2023
  • The copper ion, Cu(II), binding sites for amyloid fragment Aβ1-16 (=Aβ16 ) were investigated to explain the biological activity difference in the Aβ16 aggregation process. The [M+Cu+(z-2)H]z+ (z = 2, 3 and 4, M = Aβ16 monomer) and [D+Cu+(z-2)H]z+ (z = 3 and 5, D = Aβ16 dimer) structures were investigated using electrospray ionization (ESI) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Fragment ions of the [M+Cu+(z-2)H]z+ and [D+Cu+(z-2)H]z+ complexes were observed using collision-induced dissociation MS/MS. Three different fragmentation patterns (fragment "a", "b", and "y" ion series) were observed in the MS/MS spectrum of the (Aβ16 monomer or dimer-Cu) complex, with the "b" and "y" ion series regularly observed. The "a" ion series was not observed in the MS/MS spectrum of the [M+Cu+2H]4+ complex. In the non-covalent bond dissociation process, the [D+Cu+3H]5+ complex separated into three components ([M+Cu+H]3+, M3+, and M2+), and the [M+Cu]2+ subunit was not observed. The {M + fragment ion of [M+Cu+H]3+} fragmentation pattern was observed during the covalent bond dissociation of the [D+Cu +3H]5+ complex. The {M + [M+Cu+H]3+} complex geometry was assumed to be stable in the [D+Cu+3H]5+ complex. The {M + fragment ion of [M+Cu]2+} fragmentation pattern was also observed in the MS/MS spectrum of the [D+Cu+H]3+ complex. The {M + [y9+Cu]1+} fragment ion was the characteristic fragment ion. The [D+Cu+H]3+ and [D+Cu+3H]5+ complexes were likely to form a monomer-monomer-Cu (M-M-Cu) structure instead of a monomer-Cu-monomer (M-Cu-M) structure.

The Effect of Irrigation Concentration on the Growth and Fruit Quality of Sweet Pepper(Capsicum annuum L.) in Fertigation (관비재배에서 급액농도가 착색단고추의 생육과 품질에 미치는 영향)

  • 배종향;김귀호
    • Journal of Bio-Environment Control
    • /
    • v.13 no.3
    • /
    • pp.167-171
    • /
    • 2004
  • Objective of this research was to investigate the effect of irrigation concentration on the growth and fruit quality of sweet pepper(Capsicum annuum L.) in fertigation. The sweet pepper was grown for 210 days with irrigation concentration of EC 0.5, 1.0, 1.5, 2.0, and 3.0 dSㆍ$m^{-1 }$ in fertigation nutrient solution developed by European Vegetable R & D Center, Belgium. The net $CO_2$ assimilation and transpiration rate were the highest in the treatment of 2.0 dSㆍ$m^{-1 }$. The pH in the soil was range of 5.63 ~6.03, the EC increased as the irrigation concentration was getting higher. The SPAD value also increased as the irrigation concentration was getting higher, N, P, K, Mg except Ca were highest in the treatment of EC 2.0 dSㆍ$m^{-1 }$. The growth was good in the treatment of EC 2.0 dSㆍm$m^{-1 }$. The fruit length, width, firmness, and pericarp thickness had no statistical differences among treatments, the fruit fresh weight and dry weight were good in the treatment of EC 2.0 dSㆍ$m^{-1 }$ the yield was good in the treatment of EC 1.5 dSㆍ$m^{-1 }$ and EC 2.0 dSㆍ$m^{-1 }$ The sugar contents was the highest in the treatment of EC 2.0 dSㆍ$m^{-1 }$ with 9.0$^{\circ}$Brix. In conclusion, the optimal irrigation concentration for sweet pepper fertigation was EC 2.0 dSㆍm$^{-1}$ .

Characterization of D-Xylose Isomerase from Streptomyces albus (Stleptomyces albus의 D-Xylose Isomerase의 성질에 관하여)

  • 김영호;하영칠
    • Korean Journal of Microbiology
    • /
    • v.16 no.2
    • /
    • pp.47-61
    • /
    • 1978
  • Strptomyces albus T-12 which ahd been isolated and identified in the laboratory, was selected for the studies on the cultural conditions on the production of D-xylose iosmerase and the enzymological characteristics using the partially purified enzyme. The best results in the enzyme production came from D-xylose medium than wheat bran. The divalent metla ions as $Co^{2+},\;Fe^{2+},\;Zn^{2+}\;and\;Cu^{2+}$ retard or inhibit the cell-growth at the early stages of mycelia propagations, and T-12 strain is especially sensitive to $Co^{2+}$. After 60 hours of shaking cultivation at $30^{\circ}C$ and 200 rpm, a maximum enzyme activitz, 0.49 enzyme units, was obtained. Cell-free enzyme obtained from mycelia heat-treated in the prescence of 0.5mM $Co^{2+}$, showed a 2.4-fold increase in specific than the enzyme from untreated mycelia. The specific activity of the purified enzyme through Sephadex G-150 columm showed 180 fold to the crude enzyme. The effective activators of the enzyme appeared to be $Mg^{2+}\;and\;Co^{2+}$ ions, and it exhibited the maximal enzyme activity showed at pH 7.0 and at tempersture around $80^{\circ}C$ when $Mg^{2+}\;and\;Co^{2+}$ ions were added. The enzyme isomerized D-glucose, D-xylose, D-ribose, L-arabinose, D-mannose, and L-rhamnose in the present of $Mg^{2+}\;and\;Co^{2+}$ ions as an activatiors. $Mg^{2+}\;and\;Co^{2+}$ ions were non-competitively bound at different allosterix sites of enzyme molecule. $Mg^{2+}(5mM)\;or\;Co^{2+}(1.0mM)$ protected against the thermal denaturations of the enzyme activities. The michelis constant(Km) and $V_{max}$ values of the emzyme for D-glucose and D-xylose were 0.52M, $2.12{\mu}moles/ml{\cdot}min.\;and\;0.28M,\;0.65moles/ml{\cdot}min.$, respectively.

  • PDF

Herbicidal Activity of Naturally Developed d-Limonene against Sicyos angulatus L. under the Greenhouse and Open Field Condition (온실 및 노지조건에서 천연물질 d-Limonene의 가시박에 대한 살초활성)

  • Kang, Chung-Kil;Oh, Young-Joo;Lee, Sang-Beom;Lee, Byung-Mo;Nam, Hong-Shik;Lee, Yong-Ki;Jee, Hyeong-Jin;Hong, Moo-Ki;Koo, Suk-Jin
    • Korean Journal of Weed Science
    • /
    • v.31 no.4
    • /
    • pp.368-374
    • /
    • 2011
  • This experiment was conducted to Investigate the herbicidal activity of naturally developed d-Limonene on the control of Sicyos angulatus L. which is destructive to biodiversity and ecosystem under greenhouse and open field condition. Among the tested concentrations, most efficacy of early foliar application(leaf stage with 1.5 and 3) of d-Limonene was found in $70mg\;mL^{-1}$, followed by $35mg\;mL^{-1}$, $17.5mg\;mL^{-1}$ in that order under greenhouse condition. The higher the d-Limonene concentration, the better the effect in greenhouse condition. In leaf stage with 2, 3 and 5 under open field condition, the higher the d-Limonene concentrations, the higher the weeding effect. The remarkable effect was, particularly, found in d-Limonene with $70mg\;mL^{-1}$ while little was found in the plants with d-Limonene with $35mg\;mL^{-1}$. In the case of 15 leaf stage of S. angulatus L. treated with d-Limonene under outside treatment effect was low. The treatment effect of d-Limonene under open field compared with greenhouse condition was more significant. It can be recommended that d-Limonene with $70mg\;mL^{-1}$ could control S. angulatus L. by 5 leaf stage.

M-Zn (M = Sb, V, and Nb) Substituted Strontium Hexaferrites with Enhanced Saturation Magnetization for Permanent Magnet Applications

  • Sapoletova, Nina;Kushnir, Sergey;Ahn, Kyunghan;An, Sung Yong;Choi, Moonhee;Kim, Jae Yeong;Choi, Changhak;Wi, Sungkwon
    • Journal of Magnetics
    • /
    • v.21 no.3
    • /
    • pp.315-321
    • /
    • 2016
  • M-Zn (M = Sb, V, Nb) substituted M-type strontium hexaferrites were prepared by a ceramic method. The phase composition, morphology and magnetic properties were studied by x-ray diffractometry, scanning electron microscopy and vibrating sample magnetometry. Saturation magnetization increases with a substitution up to 75.0 emu/g (2.5 % higher compared to unsubstituted hexaferrite) and then decreases with a further substitution. A coercive field of substituted hexaferrite powders with highest saturation magnetization is more than 3 kOe. Substituted strontium hexaferrite powders prepared in this work are a rare example of high $M_S$ compositions without doping rare-earth elements and would be a promising candidate for a permanent magnet application.

A New Systolic Array for LSD-first Multiplication in $CF(2^m)$ ($CF(2^m)$상의 LSD 우선 곱셈을 위한 새로운 시스톨릭 어레이)

  • Kim, Chang-Hoon;Nam, In-Gil
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.33 no.4C
    • /
    • pp.342-349
    • /
    • 2008
  • This paper presents a new digit-serial systolic multiplier over $CF(2^m)$ for cryptographic applications. When input data come in continuously, the proposed array produces multiplication results at a rate of one every ${\lceil}m/D{\rceil}$ clock cycles, where D is the selected digit size. Since the inner structure of the proposed array is tree-type, critical path increases logarithmically proportional to D. Therefore, the computation delay of the proposed architecture is significantly less than previously proposed digit-serial systolic multipliers whose critical path increases proportional to D. Furthermore, since the new architecture has the features of regularity, modularity, and unidirectional data flow, it is well suited to VLSI implementations.