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Microtine Rodent-Borne Hantavirus from Poland and Korea: Molecular Characterization and Phylogenetic Analysis (Tula 한타바이러스의 분자생물학적 특성분석 및 국내 밭쥐아과 설치류가 매개하는 새로운 한타바이러스)

  • Song, Jin-Won;Yoon, Jae-Kyung;Kim, Sang-Hyun;Kim, Jong-Hun;Lee, Young-Eun;Song, Ki-Joon;Baek, Luck-Ju;Kordek, Radzislaw;Liberski, Pawel P.;Yanagihara, Richard;Lee, Yong-Ju
    • The Journal of Korean Society of Virology
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    • v.28 no.3
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    • pp.275-285
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    • 1998
  • Based on the geographic range and distribution of its rodent reservoir host, the European common vole (Microtus arvalis), Tula virus is likely to be widespread throughout Eurasia. Tula virus-infected voles have been captured in Central Russia, Austria, Czech and Slovak Republics, and the former Yugoslavia. Although serologic evidence for Hantaan (HTN) or Seoul (SEO) virus infection can be found in the vast majority of the more than 300 cases of hemorrhagic fever with renal syndrome (HFRS) occurring annually in Korea, approximately 4% of Korean patients with HFRS show a more than 4-fold higher antibody titer to Puumala (PUU) virus than to HTN or SEO virus by double-sandwich IgM ELISA, suggesting the existence of pathogenic Puumala-related hantaviruses in Korea. To further define the geographic distribution and genetic diversity of Tula virus in Eurasia and to investigate the existence of previously unrecognized Microtus-borne hantavirus in Korea, arvicolid rodents were captured in Lodz, Poland in 1995 and in Yunchon-kun, Kyungki-do during April to May, 1998. In addition, sera from 18 Korean HFRS patients who showed higher (or the same) antibody titer to Tula virus than HTN and SEO viruses were examined for hantavirus RNA by RT-PCR. Hantaviral sequences were not detected in any of the 18 patients or in 35 reed voles (Microtus fortis) in Korea. Alignment and comparison of a 208-nucleotide region of the S segment, amplified from lung tissues of two hantavirus-seropositive Marvalis captured in Poland, revealed $80.8{\sim}83.2%$ sequence similarity, respectively, with Tula virus strains from Central Russia and the Czech and Slovak Republics. Phylogenetic analysis indicated that the newfound Tula virus strains from Poland were closely related to other Tula hantaviruses from Eurasia.

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EU Enlargement toward Central and Eastern Europe, Location of Foreign Direct Investment and the Changing of Economic Space (EU의 중.동부유럽으로의 확대와 투자입지 및 경제공간의 변화)

  • Moon, Nam-Cheol
    • Journal of the Korean association of regional geographers
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    • v.10 no.4
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    • pp.698-712
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    • 2004
  • As EU's enlargement toward Central and Eastern countries, it is expected to change of the economic activity space of EU. This paper aims to examine the changing of economic activity space of EU with a locational analysis of foreign direct investment in Central and Eastern countries. The foreign enterprises, particularly EU's firms are increasing the direct investment in the Central and Eastern countries from the middle 1990's for a raising of the efficiency of production and a prior occupation of the potential market. The foreign direct investment in Central and Eastern Europe has a tendency to concentrate in Poland, Hungary, Czech and Slovakia, which has relatively a large market, a cheap and plentiful labor, a stable political system and a positive inviting policy of foreign direct investment. And, the foreign direct investment shows a tendency to locate generally in a big city, which has a advantageous production factors by a well-developed infrastructure and a regional industrial organization, a skilled labors, a market, etc., and in a border area between East and West Europe because of a cheap and plentiful labor, a similar cultural environment and the low cost of transportation. In conclusion, as an integration of the Central and Eastern countries into EU, the capital territories of Hungary, Czech and Poland, and the border area between East and West Europe is becoming a new economic activity space of EU with a location of foreign direct investment. And. the Baltic zone is also expect to become a new economic activity space according to the augmentation of exchanges and direct investment inter near countries.

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A Study on the Integrated Type of the Cadastral System and Land Registration System in Foreign Countries (지적제도와 토지등기제도의 통합 사례에 관한 연구)

  • Ryu, Byoung-chan
    • Journal of Cadastre & Land InformatiX
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    • v.50 no.1
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    • pp.143-162
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    • 2020
  • The purpose of this study is to derive the type by examining and analyzing the integrated cases of the Cadastral System (hereafter as CS.) and the Land Registration System (hereafter as LRS.) except for France, the Netherlands, Japan, and Taiwan introduced in Korea. As a result of the study, it was confirmed that the CS. and LRS. were integrated in Turkey, Indonesia, Hungary, Czech Republic, and Lithuania, and the following three types were drawn as a result of analyzing the integration case. First, the French type, the CS. and LRS. has been integrated since the establishment of the system, and the central and state governments are managed by one Administrative Agency, The basic municipalities include France and Turkey, which are managed by two separate agencies. Second, the Dutch type, there are Netherlands, Indonesia, and Lithuania, which are integrated and managed by one Administrative Agency since the creation of the CS. and LRS. Third, the Japanese type, there are Japan, Taiwan, Hungary, and Czech Republic which was separated from the creation of the CS. and the LRS. and the CS. was managed by the Administrative Agency and the LRS. was managed by the Judicial Agency. but after integrated both systems were managed by one Administrative Agency, Furthermore, CS. was managed by the Administrative Agency in all countries but LRS. was managed by the Judicial Agency in some countries. but after integrated both systems were managed by one Administrative Agency. I hope that active research on the integration of the CS. and LRS. will be carried out in the future, and hope that the results of this study will be used as basic data for research on how to integrate the CS. and LRS. in Korea.

Influence of Temperature on Microsporidian Multiplication and Spore Production in Various Tissues of Silkworm (Bombyx mori L.) During Larval Development

  • Mohanan N. Madana;Krishnan N.;Mitra P.;Das K.K.;Saratchandra B.;Haldar D.P.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.12 no.2
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    • pp.87-93
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    • 2006
  • Multiplication and spore production of three microsporidia viz., Nosema bombycis, Nosema sp. 1 and Nosema sp. 2 in fifth instar larval tissues of silkworm, Bombyx mori L. in two seasons with distinct temperature regimes were studied. Nosema sp. 2 produced significantly (P < 0.01) higher number of spores in various tissues. Among the tissues, spore production was highest in silk gland, followed by fat body and gut. Spore production was significantly (P < 0.01) higher in season-II (Average temperature $29.4{\pm}1.1^{\circ}C$). Maximum spore production was observed 25 days post inoculation (p. i.) in season-I (Average temperature $18.9{\pm}1.1^{\circ}C$), whereas in season-II, it was 14 days p. i. In season-I, spore production was low up to 21 days p. i., then increased sharply. In season-II, there was a steady increase in spore production. The results indicate that the microsporidian multiplication is tissue specific and extremely sensitive to temperature at which the host is reared. It also reveals that, silk gland, fat body and gut are the most appropriate tissues for microscopic identification of microsporidia in the larval stage.

Association between I/D Polymorphism of Human LRPAP1 Gene and Body Mass Index in Korean General Population

  • Kang, Byung-Yong;Bae, Hak-Gyoon;Jhin, Hae-Kyung;Lee, Kyung-Soon;Lee, Kang-Oh
    • Toxicological Research
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    • v.19 no.3
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    • pp.205-210
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    • 2003
  • The aim of this study was to estimate the influence of 37 bp insertion/deletion (I/O) poly-morphism of the low density lipoprotein receptor-related protein-associated protein 1 (LRPAP1) gene on anthropometrical or biochemical parameters in korean general population. To determine the frequency of the genotype, we analyzed 244 samples of Korean origin. The frequency of the I allele was 0.55 in men and 0.56 in women, which were significantly higher than the frequency (0.26) that was reported in Czech population of Caucasian origin. In addition, the I allele of this polymorphism was significantly associated with higher value of body mass index (BMI) in our subjects by ANOVA test (P<0.05), and this association was maintained after controlling for age and gender by ANCOVA test (P<0.05). Thus, our results suggest that the I/O polymorphism of the LRPAP1 gene may be useful as a genetic marker for obesity in Korean general population.

Seasonal Impact of Microsporidian Infection on the Reproductive Potential of Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae)

  • Mohanan N. Madana;Krishnan N.;Mitra P.;Das N. K.;Saratchandra B.;Haldar D. P.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.2
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    • pp.107-111
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    • 2005
  • Impact of microsporidian infection and season on reproductive potential of Bombyx mori L. was investigated in the laboratory. Microsporidian infection significantly (P<0.01) reduced fecundity and hatching and increased sterility and mortality of eggs. Among the microsporidia, Nosema sp. 2 infected silkworm produced eggs with least fecundity and hatching percent as well as highest dead and sterile eggs followed by Nosema sp. 1 and N. bombycis. Microsporidia, in general, significantly reduced fecundity and hatching percent of eggs and increased number of dead and sterile eggs in all the three seasons except N. bombycis in July - August (S3) and Nosema sp. 2 in January February (S1). Since, seed production is the anchor sheet of mulberry sericulture, coefficient of egg lying is considered as an important aspect and the industry quite often facing shortage of disease free layings. The present study indicates that B. mori is more susceptible to microsporidia during S2 followed by S3 and S1 and Nosema sp2. is most virulent followed by Nosema spl. and N. bombycis.

Papaya Ringspot Virus Coat Protein Gene for Antigen Presentation in Escherichia coli

  • Chatchen, Supawat;Juricek, Mila;Rueda, Paloma;Kertbundit, Sunee
    • BMB Reports
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    • v.39 no.1
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    • pp.16-21
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    • 2006
  • The coat protein (CP) of Papaya ringspot virus (PRSV) was analyzed for presentation of the antigenic peptide of animal virus, Canine parvovirus (CPV), in Escherichia coli (E. coli). The 45 nucleotides fragment coding for the 15-aa peptide epitope of the CPV-VP2 protein was either inserted into the PRSV-cp gene at the 5', 3' ends, both 5' and 3' ends or substituted into the 3' end of the PRSV cp gene. Each of the chimeric PRSV cp genes was cloned into the pRSET B vector under the control of the T7 promoter and transformed into E. coli. The recombinant coat proteins expressed from different chimeric PRSV-cp genes were purified and intraperitoneally injected into mice. All of the recombinant coat proteins showed strong immunogenicity and stimulate mice immune response. The recombinant coat proteins containing the CPV epitope insertion at the C terminus and at both N and C termini elicited ten times higher specific antisera in immunized mice compared with the other two recombinant coat proteins which contain the CPV epitope insertion at the N terminus and substitution at the C terminus.

Electrical and Thermo-mechanical Properties of DGEBA Cycloaliphatic Diamine Nano PA and SiO2 Composites

  • Trnka, Pavel;Mentlik, Vaclav;Harvanek, Lukas;Hornak, Jaroslav;Matejka, Libor
    • Journal of Electrical Engineering and Technology
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    • v.13 no.6
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    • pp.2425-2433
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    • 2018
  • This study investigates a new organic based material and its dielectric and mechanical properties. It is a comprehensive nanocomposite comprising a combination of various types of nanofillers with hydrophobic silica nanoparticles (AEROSIL R 974) as a matrix modifier and a polyamide nano nonwoven textile, Ultramid-Polyamide 6, pulped in the electrostatic field as a dielectric barrier. The polymer matrix is an epoxy network based on diglycidyl ether of bisphenol A (DGEBA) and cycloaliphatic diamine (Laromine C260). The designed nanocomposite material is an alternative to the conventional three-component composites containing fiberglass and mica with properties that exceed current electroinsulating systems (volume resistivity on the order of $10^{16}{\Omega}{\cdot}m$, dissipation factor tan ${\delta}=4.7{\cdot}10^{-3}$, dielectric strength 39 kV/mm).

The Implications on Healthcare System of the Unified Korea: Lesson from System Integration Countries (체제통합국 건강지표 비교를 통한 통일 후 보건의료에 대한 시사점)

  • Jun, Yeong;Huh, Sung-Eun;Lee, Joo Eun
    • Health Policy and Management
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    • v.30 no.3
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    • pp.301-310
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    • 2020
  • Background: In this study, we aimed to investigate the recent trends for health care indicators including maternal mortality ratio, infant mortality rate, under-five mortality rate, life expectancy, years of life lost, and healthcare resources in South Korea, North Korea, Germany, Russian Federation, Mongolia, Vietnam, China, Czech Republic, Poland, and Hungary. Methods: We used data from five sources: World Health Organization, Federal Institute for Population Research, World Bank, Organization for Economic Cooperation and Development health statistics, and national statistics. Results: In the early 1990s, health indicators continued to improve in countries that switched to the health insurance system, but the gap widened in North Korea as health indicators worsened. Conclusion: The establishment of a sustainable health care system after unification of the Korean peninsula requires substantial changes in the health care system and efforts to improve the health of North Koreans.

A Study of Long-term Development Plan of Korea Research Institute for Library and Information ("도서관연구소" 중장기 발전방안 연구)

  • Yoon, Hee-Yoon
    • Journal of Korean Library and Information Science Society
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    • v.39 no.2
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    • pp.5-27
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    • 2008
  • The purpose of this paper Is to present a long-term development plan for the KRILI(Korea Research Institute for Library and Information). To do so, authors analysed a various library research institutes and related organizations in U.S.A., U.K., Czech Republic, Hungary, and Japan. And using the SWOT analysis, author identified and described the current status and also surveyed recognition of library and information science faculties as to desirable roles and phase of the KRILI. Based on the results of analysis and survey, this paper suggested a long-term plan(vision and objectives, strategic issues, desirable phase and organization system, growth and development model, Internal cooperative operating systems and external research cooperation system, etc.) of the KRILI.

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