• Title/Summary/Keyword: Cytotoxic

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Steroids and Triterpenoid from the Fruit Bodies of Ganoderma lucidum and Their Cytotoxic Activity

  • Lee, Joon-Seok;Lee, Mi-Kyoung;Hung, Tran-Manh;Lee, Ik-Soo;Min, Byung-Sun;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.15 no.3
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    • pp.173-179
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    • 2009
  • To determine the cytotoxic activity of natural compounds, chromatographic separation of the hexanesoluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of four steroids and one triterpenoid. They were identified as ergosterol peroxide (1), stella sterol (2), ergosterol (3), 9(11)-dehydroergostrol peroxide (4), and ganodermanontriol (5) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their cytotoxic activity against HL-60, MCF-7, and LLC cancer cell lines. Ganodermanontriol (5) showed cytotoxic activity with IC$_{50}$ values of 24.8 and 22.9 $\mu$g/mL against HL-60 and MCF-7 cancer cell lines, respectively, whereas compounds 1 - 4 were inactive.

Lupane Derivatives-I: Synthesis and Cytotoxic Activity (루판 유도체의 합성 및 세포독성-I)

  • You, Young-Jae;Kim, Yong;Ahn, Byung-Zun
    • YAKHAK HOEJI
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    • v.46 no.5
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    • pp.295-300
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    • 2002
  • To observe the structure-activity relationship of lupane derivatives both on cytotoxic and antiangiogenic activity, twelve lupane derivatives were prepared; their antiangiogenic and cytotoxic activities were evaluated. Among them, four compounds were more cytotoxic than betulinic acid. Carboxylic acid at C28 seemed to be essential for cytotoxic activity. But, a selective cytotoxicity toward SK-MEL-2 was not observed. As for antiangiogenic activity, none of the compounds except lupeol showed antiangiogenic activity at 30 $\mu\textrm{g}$/mL.

Cytotoxicity of Daucus Carota L. on Various Cancer Cells (인체 암세포에 대한 당근 추출 성분의 세포독성효과)

  • 배송자;한은주;노승배
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.153-160
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    • 2000
  • We investigated the cytotoxic effects of roots and seeds Daucus carota L. on HepG2, HeLa, MCF7, SW626, C6 and NB41A3 cell lines by the MTT assay. Among extracts, the ethylacetate partition layer (DCMEA) of root of Daucus carota L. showed the strongest cytotoxic effects on HepG2, HeLa, C6 and NB41A3 cell lines. On the other hand, methanol(DCM), n-ethylacetate(DCMEA) and n-butanol(DCMB) extract of the seeds of Daucus carota L. also showed significant cytotoxic activities for all six cell lines. $\beta$-carotene, a well-known main component of Daucus carota L. was also tested for its cytotoxic effect. However, in all six cell lines, $\beta$-carotene falied to show significant cytotoxicity. Therefore, the anticancer effect of DCMEA of root fo Daucus carota L. and DCM, DCMH, DCMEA and DCMB extracts of seeds may be caused by components other than $\beta$-carotene. Futher studies are under way to isolate the compounds responsible for the significant cytotoxic activity.

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Cytotoxic Constituents from Boesenbergia pandurate (Roxb.) Schltr

  • Ching, Amy Yap Li;Lian, Gwendoline Ee Cheng;Rahmani, Mawardi;Khalid, Kaida;Sukari, Mohd Aspollah
    • Natural Product Sciences
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    • v.13 no.2
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    • pp.110-113
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    • 2007
  • Five flavonoid derivatives, pinostrobin (1), pinocembrin (2), alpinetin (3), cardamonin (4) and boesenbergin A (5) were isolated from the rhizomes of Boesenbergia pandurata. All compounds were elucidated based on its spectroscopic data and by the comparison with the previous works. 2D NMR technique was used for the structure elucidation of boesenbergin A to complement the data reported previously. The extracts and pure compounds were screened for cytotoxic activity against HL-60 cancer cell lines (human promyelocytic leukemia). Cytotoxic screening showed most of the extracts and pure compounds isolated from the rhizomes of Boesenbergia pandurata were active against HL-60 cancer cell line. The chloroform extract and boesenbergin A showed the most potent cytotoxic activity.

Cytotoxic Lactones from the Pericarps of Litsea japonica

  • Ngo, Quynh-Mai Thi;Cao, Thao Quyen;Woo, Mi Hee;Min, Byung Sun
    • Natural Product Sciences
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    • v.25 no.1
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    • pp.23-27
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    • 2019
  • From the pericarps of Litsea japonica (Thunb.) Jussieu, eighteen butanolide derivatives (1 - 18) were evaluated for their cytotoxic activity against HeLa, HL-60, and MCF-7 cells. Compounds 1-9 with 2-alkylidene-3-hydroxy-4-methylbutanolides structure exhibited cytotoxic activities against cancer-cell lines. Among them, compound 8 (litsenolide $D_2$) exhibited the most potent cytotoxicity against the tested cell lines, including HeLa, HL-60, and MCF-7, with $IC_{50}$ values of $17.6{\pm}1.3$, $4.2{\pm}0.2$, and $12.8{\pm}0.0{\mu}M$, respectively. Compound 8 induced apoptosis in a dose-dependent manner. Annexin V/Propidium Iodide (PI) double staining confirmed that 8 effectively induced apoptosis in MCF-7 cells. To the best of our knowledge, we have reported cytotoxic activity of butanolides from L. japonica against these cancer-cell lines for the first time.

Cytotoxic activity of processing the traditional drug on monkey kidney cell (Vero) and human liver cell (WRL68) (수치(修治) 한약재가 사람의 간세포 WRL68와 원숭이의 신장세포 Vero에 미치는 영향)

  • Ju Young-Sung;Kim Ho-Kyoung;Ko Byoung-Seob
    • Journal of Society of Preventive Korean Medicine
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    • v.4 no.2
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    • pp.258-272
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    • 2000
  • The cytotoxic activities of Pinellia ternate, Aconitum carmichaeli, Arisaema amurense, Aconitum kusnezoffii and Scutellaria baicalensis on monkey kidney cell (Vero) and human liver cell (WRL68) were evaluated by Sulforhodamine B Protein (SRB) and Tetrazolium-based (MTT) colorimetric assay methods The results were as fellows : 1 The Pinellia ternate and Arisaema amurense did not show the cytotoxic activities at any concentration without processing or natural drugs. 2 The extracts of Aconitum carmichaeli, Aconitum kusnezoffii and Scutellaria baicalensis showed cytotoxic activities. However, the cytotoxic activities of processing drugs were less effective than the natural drugs. 3. The cytotoxic activities on monkey kidney cell (Vero) and human liver cell (WRL68) of Aconitum carmichaeli was determined by MTT assay. The Kyungpo(京?) of Aconitum carmichaeli showed less concentrate than that of the Dangpo(唐?). The $IC_{50}$ value on monkey kidney cell (Vero) and human liver cell (WRL68) of Kyungpo was $937{\pm}29\;and\;731{\pm}31{\mu}g/ml$, respectively 4. The cytotoxicity on monkey kidney cell (Vero) and human liver cell (WRL68) of Scutellaria baicalensis showed strong activities without processing or natural drugs.

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Cytotoxic Triterpenoids from the Fruits of Ligustrum japonicum

  • Thi Ngo, Quynh-Mai;Cao, Thao Quyen;Woo, Mi Hee;Min, Byung Sun;Weon, Kwon-Yeon
    • Natural Product Sciences
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    • v.24 no.2
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    • pp.93-98
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    • 2018
  • Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 - 13) were isolated and evaluated for their cytotoxic activity against Hela and HL-60 cells. As results, 8 (oleanolic acid) showed significant effects on Hela with $IC_{50}$ values of $5.5{\mu}M$, and moderate effects on HL-60 cells with $IC_{50}$ values of $55.9{\mu}M$. Meanwhile, 10 (oleanderic acid) and 11 ($3{\beta}$-acetoxy-urs-12-en-28-oic acid) exhibited moderate inhibitory effects on Hela with $IC_{50}$ value of 55.0 and $68.8{\mu}M$, respectively. Moreover, 10 showed cytotoxic effect on HL-60 cell line with $IC_{50}$ value of $63.9{\mu}M$. To our knowledge, this is the first report that oleanderic acid was isolated from L. japonicum and investigated in cytotoxic effects on Hela and HL-60 cells.

Cytotoxic Effects of Strawberry, Korean Raspberry, and Mulberry Extracts on Human Ovarian Cancer A2780 Cells

  • Lee, Dahae;Kang, Ki Sung;Lee, Sanghyun;Cho, Eun Ju;Kim, Hyun Young
    • Preventive Nutrition and Food Science
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    • v.21 no.4
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    • pp.384-388
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    • 2016
  • Reactive oxygen species are tumorigenic by their ability to increase cell proliferation, survival, and cellular migration. The purpose of the present study was to compare the antioxidant activity and cytotoxic effects of 3 berry extracts (strawberry, Korean raspberry, and mulberry) in A2780 human ovarian carcinoma cells. Except for raspberry, the ethyl acetate or methylene chloride fractions of berries containing phenolic compounds exerted dose dependent free radical scavenging activities. In the raspberry fractions, the hexane fraction also exhibited potent antioxidant activity. The cytotoxic effects of berries extracts in A2780 human ovarian carcinoma cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Surprisingly, co-treatment with n-butanol (BuOH) fractions of berries showed stronger cytotoxic effects compared to the other fractions. These findings suggest that potent anticancer molecules are found in the BuOH fractions of berries that have stronger cytotoxic activity than antioxidants.

Effects of Education for Safe Handling of Cytotoxic Drugs on Knowledge and Practice in Oncology Nurses (항암제 안전관리 교육이 간호사의 안전관리 수칙 지식 및 실천에 미치는 효과)

  • Hong, Su-Youn;Cho, Eun-Jung;Yoo, Yang-Sook
    • Asian Oncology Nursing
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    • v.6 no.1
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    • pp.47-53
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    • 2006
  • Purpose: The present study explored the effects of education for safe handling of cytotoxic drugs on knowledge and practice in nurses. Method: Data were collected using a single group with pre and posttest design from 59 nurses at medical and surgical wards in C university hospital from April to June 2005. Questionnaires on the knowledge and practice of the guidelines for safe handling of cytotoxic drugs were asked to complete before and after the education. Result: The education improved nurses' knowledge and practice of the guidelines for safe handling of cytotoxic drugs. The knowledge and practice had a significant positive correlation. Conclusion: These findings show that the education for safe handling of cytotoxic drugs improved the knowledge and practice of nurses. As the knowledge of the guidelines for safe handling of cytotoxic drugs makes a positive association with the practice, it is thought to be required to enhance the education program for nurses.

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Cytotoxic Effects of Furanosesterterpenes, Cyclitol Derivatives, and Bromotyrosine Derivative Isolated from Marine Sponges

  • Sohn, Jae-Hak;Oh, Hyun-Cheol;Jung, Jee-H.;Bae, Song-Ja
    • Preventive Nutrition and Food Science
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    • v.10 no.3
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    • pp.257-261
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    • 2005
  • Marine sponges are known to produce a number of cytotoxic secondary metabolites. In the course of searching for cytotoxic metabolites from marine organisms, we have evaluated cytotoxic activities of six marine secondary metabolites isolated from various sponges. The cytotoxic compounds 1-6 were isolated by the application of various chromatographic methods, including column chromatography and HPLC. The molecular structures were mostly determined using mass spectrometry (MS) and Nuclear Magnetic Resonance (NMR) Spectroscopy. Furanosestererpenes (compounds 1-3) from Psammocinia sp., cyclitol derivatives (compounds 4 and 5) from Sarcotragus sp., and bromotyrosine-type compound (6) from an association of two sponges Jaspis wondoensis and Poecillastra wondoensis were evaluated for their cytotoxic activity against three cancer cell lines; Hep G2, HeLa, and MCF-7. All tested compounds exhibited cyctoxicity at concentrations ranging from $5\;\mug/mL\;to\;25\;\mug/mL.$ Particularly, among the tested compounds, compound 6 showed the highest potency displaying at least $80\%$ of cytotoxicity at $5\;\mug/mL$ level against all three cancer cell lines.