• 한국수산과학회지
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• 제32권3호
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• pp.317-319
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• 1999

해산어 종묘 생산과정에서 초기 먹이생물로서 이용되는 로티퍼의 대량 배양조에서 Vorticella는 로티퍼의 증식을 향상시킬 뿐만 아니라, 배양조내에서 로티퍼의 증식을 억제시키는 유해한 섬모충과 세균의 증식을 억제한다. 이 연구에서는 Vorticella cyst의 형성과 보존에 MCCF(Marine Ciliate Cyst Formation) 배지가 이용 가능함을 알 수 있었다. Vorticella의 cyst 형성 과정은 MCCF 배지내의 사육수가 증발함에 따라 여과 섭식 활동의 정지, 배지 표면에의 잠복, 스파스모넴의 탈락 그리고 Cyst의 형성의 순으로 진행되었다. 그리고 1년에 걸친 장기 보존후에도 배지중에 해수를 첨가하는 간단한 방법만으로 활동형 세포에의 복귀가 가능했다. 형성된 Vorticella cyst의 모양은 한쪽 길이가 다른 한쪽의 길이 보다 긴 타원형을 하고 있었으며, cyst의 크기는 장경이 $30.51 \pm1.98\;\mu$m (Avg. $\pm$ SD로 $28.35\~32.40\mu$m의 범위)이였고, 단경은 $28.89 \pm2.12\;\mu$m(범위는 $27\~32.40\mu$m)이였다. cyst로부터 활동형 세포로 복귀한 Vorticella sp.는 배양중인 배지내에 로티퍼 사육수의원심 분리 상등액을 넣어줌으로 16일간의 배양 기간중 최고 밀도가 912 $\pm$64개체/10ml (Avg. $\pm$SD)까지 증식하였다. 이 실험에서 Vorticella의 cyst 형성과 종 보존을 위하여 사용한 MCCF 배지는 cyst를 형성시키고 보존하는데 이용 가능한 배지임을 알 수 있다. 더욱이 Vorticella와 같은 해산 섬모충의 cyst를 손쉽게 만들 수 있는 유용한 방법을 이 연구를 통하여 제시 가능하였다.

• BMB Reports
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• 제45권3호
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• pp.189-193
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• 2012

Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation.

• Journal of Korean Neurosurgical Society
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• 제39권4호
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• pp.310-313
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• 2006

Although lipomyelomeningocele and dermoid cyst are formed during a similar embryologic developmental stage of 'neural tube formation', they are caused by entirely different embryologic mechanisms. We encountered a case, which has not been previously reported, that had a lumbar subcutaneous dermoid cyst associated with lipomyelomeningocele. A 52-year-old man presented with a slowly growing lumbar mass of 3 years duration, which had been present since birth. Lumbosacral magnetic resonance imaging[MRI] showed lipomyelomeningocele at the L3, 4 and 5 levels and a subcutaneous cystic mass of high signal intensity on the T2 weighted image and iso or low signal intensity on the T1 weighted image. He underwent total resection of the lumbar subcutaneous mass. Intraoperative findings and histological examination were consistent with the preoperative diagnosis of a 'dermoid cyst'. We demonstrate that the formation of a dermoid cyst might coexist with lipomyelomeningocele during the embryologic developmental stage.

• BMB Reports
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• 제46권7호
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• pp.338-345
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• 2013

microRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by targeting the 3'-untranslated region of multiple target genes. Pathogenesis results from defects in several gene sets; therefore, disease progression could be prevented using miRNAs targeting multiple genes. Moreover, recent studies suggest that miRNAs reflect the stage of the specific disease, such as carcinogenesis. Cystic diseases, including polycystic kidney disease, polycystic liver disease, pancreatic cystic disease, and ovarian cystic disease, have common processes of cyst formation in the specific organ. Specifically, epithelial cells initiate abnormal cell proliferation and apoptosis as a result of alterations to key genes. Cysts are caused by fluid accumulation in the lumen. However, the molecular mechanisms underlying cyst formation and progression remain unclear. This review aims to introduce the key miRNAs related to cyst formation, and we suggest that miRNAs could be useful biomarkers and potential therapeutic targets in several cystic diseases.

• BMB Reports
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• 제50권9호
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• pp.460-465
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• 2017

Polycystic kidney disease (PKD) is one of the most common inherited disorders, involving progressive cyst formation in the kidney that leads to renal failure. FK506 binding protein 12 (FK506BP) is an immunophilin protein that performs multiple functions, including regulation of cell signaling pathways and survival. In this study, we determined the roles of PEP-1-FK506BP on cell proliferation and cyst formation in PKD cells. Purified PEP-1-FK506BP transduced into PKD cells markedly inhibited cell proliferation. Also, PEP-1-FK506BP drastically inhibited the expression levels of p-Akt, p-p70S6K, p-mTOR, and p-ERK in PKD cells. In a 3D-culture system, PEP-1-FK506BP significantly reduced cyst formation. Furthermore, the combined effects of rapamycin and PEP-1-FK506BP on cyst formation were markedly higher than the effects of individual treatments. These results suggest that PEP-1-FK506BP delayed cyst formation and could be a new therapeutic strategy for renal cyst formation in PKD.

• Clinics in Shoulder and Elbow
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• 제26권3호
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• pp.276-286
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• 2023

Background: The transosseous anchorless repair (ToR) technique was recently introduced to avoid suture anchor-related problems. While favorable outcomes of the ToR technique have been reported, no previous studies on peri-implant cyst formation with the ToR technique exist. Therefore, this study compared the clinical outcomes and prevalence of peri-implant cyst formation between the ToR technique and the conventional transosseous equivalent technique using suture anchors (SA). Methods: Cases with arthroscopic rotator cuff repair (ARCR) between 2016 and 2018 treated with the double-row suture bridge technique were retrospectively reviewed. Patients were divided into ToR and SA groups. To compare clinical outcomes, 19 ToR and 57 SA cases without intraoperative implant failure were selected using propensity score matching (PSM). While intraoperative implant failure rate was analyzed before PSM, retear rate, peri-implant cyst formation rate, and functional outcomes were compared after PSM. Results: The intraoperative implant failure rate (ToR, 8% vs. SA, 15.3%) and retear rate (ToR, 5.3% vs. SA, 19.3%) did not differ between the two groups (all P>0.05). However, peri-implant cysts were not observed in the ToR group, while they were observed in 16.7% of the SA group (P=0.008). Postoperative functional outcomes were not significantly different between the two groups (all P>0.05). Conclusions: The ToR technique produced comparable clinical outcomes to conventional techniques. Considering the prospect of potential additional surgeries, the absence of peri-implant cyst formation might be an advantage of ToR. Furthermore, ToR might reduce the medical costs related to suture anchors and, thereby, could be a useful option for ARCR. Level of evidence: III.

• 대한관절경학회지
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• 제12권3호
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• pp.225-228
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• 2008

전방 십자인대 재건술 후 발생하는 전경골 낭종은 드물게 발생하는 질환이다. 저자들은 전경골근 동종건을 이용한 전십자인대 재재건술 후 발생한 전경골 낭종을 보고하고자 한다. 본 합병증은 경골 터널을 통하여 관절과 낭종이 이어져 발생한 것으로 추정된다. 저자들은 전십자 인대 재건술 후 전경골 낭종의 발생 가능성이 있음을 보고하고자 한다.

• Clinics in Shoulder and Elbow
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• 제22권2호
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• pp.100-105
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• 2019

Suture anchors are commonly used in shoulder surgeries, especially for rotator cuff tears. Peri-anchor cyst formation, however, is sometimes detected on follow-up radiologic image after surgery. The purpose of this report is to discuss the case of a patient who presented with regression of extensive peri-anchor cyst on postoperative 4-year follow-up magnetic resonance imaging and had good clinical outcome despite peri-anchor cyst formation after arthroscopic rotator cuff repair.

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.361-364
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• 2012

The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose is one of the primary components of the inner cyst wall, cellulose synthesis is essential to the process of cyst formation in Acanthamoeba. In this study, we hypothesized the key and short-step process in synthesis of cellulose from glycogen in encysting Acanthamoeba castellanii, and confirmed it by comparing the expression pattern of enzymes involving glycogenolysis and cellulose synthesis. The genes of 3 enzymes, glycogen phosphorylase, UDP-glucose pyrophosphorylase, and cellulose synthase, which are involved in the cellulose synthesis, were expressed high at the 1st and 2nd day of encystation. However, the phosphoglucomutase that facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate expressed low during encystation. This report identified the short-cut pathway of cellulose synthesis required for construction of the cyst wall during the encystation process in Acanthamoeba. This study provides important information to understand cyst wall formation in encysting Acanthamoeba.

• Journal of Korean Neurosurgical Society
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• 제29권9호
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• pp.1258-1261
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• 2000

Intraspinal neurenteric cyst is rare congenital lesion derived from disorder of notochord formation. Most of these are located ventral to the spinal cord and conventional posterior approach is considered to be effective method as initial treatment modality. This cyst can recur, but the risk of reccurence after partial removal through long term follow-up has not been determined. We experienced one case of cervical neurenteric cyst which recurred after partial removal through laminectomy. The magnetic resonance imaging and postmyelography computerized tomography revealed an intradural extramedullary cystic lesion anterior to the cervical cord at the fifth cervical vertebra level. We performed anterior cervical corpectomy and cyst was totally removed. The patient's neurological symptom was improved postoperatively. Neurenteric cyst located ventrally to the cervical spinal cord should be removed through anterior route for direct visualization of the relationship between the cyst wall and the spinal cord.