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http://dx.doi.org/10.5483/BMBRep.2012.45.3.189

Mxi1 influences cyst formation in three-dimensional cell culture

Yook, Yeon-Joo (Department of Biological Science, Sookmyung Women's University)
Yoo, Kyung-Hyun (Department of Biological Science, Sookmyung Women's University)
Song, Seon-Ah (Department of Biological Science, Sookmyung Women's University)
Seo, Min-Ji (Department of Biological Science, Sookmyung Women's University)
Ko, Je-Yeong (Department of Biological Science, Sookmyung Women's University)
Kim, Bo-Hye (Department of Biological Science, Sookmyung Women's University)
Lee, Eun-Ji (Department of Biological Science, Sookmyung Women's University)
Chang, Eun-Sun (Department of Biological Science, Sookmyung Women's University)
Woo, Yu-Mi (Department of Biological Science, Sookmyung Women's University)
Park, Jong-Hoon (Department of Biological Science, Sookmyung Women's University)

Publication Information

BMB Reports / v.45, no.3, 2012 , pp. 189-193 More about this Journal

Abstract

Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation.

Keywords

Cyst formation; mIMCD-3 cell; Mxi1; 3-D culture;

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Reference

1	Yoo, K. H., Sung, Y. H., Yang, M. H., Jeon, J. O., Yook, Y. J., Woo, Y. M., Lee, H. W. and Park, J. H. (2007) Inactivation of Mxi1 induces Il-8 secretion activation in polycystic kidney. Biochem. Biophys. Res. Commun. 356, 85-90. DOI ScienceOn
2	Mai, W., Chen, D., Ding, T., Kim, I., Park, S., Cho, S. Y.,Chu, J. S., Liang, D., Wang, N., Wu, D., Li, S., Zhao, P., Zent, R. and Wu, G. (2005) Inhibition of Pkhd1 impairs tubulomorphogenesis of cultured IMCD cells. Mol. Biol. Cell. 16, 4398-4409. DOI ScienceOn
3	Nickel, C., Benzing, T., Sellin, L., Gerke, P., Karihaloo, A., Liu, Z. X., Cantley, L. G. and Walz, G. (2002) The polycystin- 1 C-terminal fragment triggers branching morphogenesis and migration of tubular kidney epithelial cells. J. Clin. Invest. 109, 481-489. DOI
4	Lin, H. H., Yang, T. P., Jiang, S. T., Yang, H. Y. and Tang, M. J. (1999) Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation. Kidney Int. 55, 168-178. DOI ScienceOn
5	Schreiber-Agus, N., Meng, Y., Hoang, T., Hou, H. Jr., Chen, K., Greenberg, R., Cordon-Cardo, C., Lee, H. W. and DePinho, R. A. (1998) Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growth. Nature 393, 483-487. DOI ScienceOn
6	Chen, D., Roberts, R., Pohl, M., Nigam, S., Kreidberg, J., Wang, Z., Heino, J., Ivaska, J., Coffa, S., Harris, R. C., Pozzi, A. and Zent, R. (2004) Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis. Am. J. Physiol. Renal. Physiol. 287, F602-611. DOI ScienceOn
7	Lanoix, J., D'Agati, V., Szabolcs, M. and Trudel, M. (1996) Dysregulation of cellular proliferation and apoptosis mediates human autosomal dominant polycystic kidney disease (ADPKD). Oncogene 13, 1153-1160.
8	Grantham, J. J., Geiser, J. L. and Evan, A. P. (1987) Cyst formation and growth in autosomal dominant polycystic kidney disease. Kidney. Int. 31, 1145-1152. DOI ScienceOn
9	Calvet, J. P. (1998) Molecular genetics of polycystic kidney disease. J. Nephrol. 11, 24-34.
10	Wilson, P. D. and Falkenstein, D. (1995) The pathology of human renal cystic disease. Curr. Top. Pathol. 88, 1-50. DOI