• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2245-2249
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• 2014

Background: Human papillomavirus is a well-established cause of the development of a variety of epithelial lesions in the cervix. However, as yet, incorporation of HPV testing into cervical cancer screening either as an adjunct or stand alone test is limited due to its cost. We therefore here ascertained the presence and type specificity of human papilloma virus (HPV) DNA in routine cervical scrapings. Materials and Methods: Cervical scrapings were collected from women attending clinics for routine Pap smear screening. HPV-DNA was detected by PCR using MY09/11 and GP5+/GP6+ primer sets and genotyping was accomplished by cycle-sequencing. Results: A total of 635 women were recruited into the study with $mean{\pm}SD$ age of $43{\pm}10.5$ years. Of these 92.6% (588/635) were reported as within normal limits (WNL) on cytology. The presence of HPV infection detected by nested MY/GP+-PCR was 4.4% (28/635). The overall prevalence of high-risk HPV (HR-HPV) in abnormal Pap smears was 53.8% (7/13). HPVs were also seen in 3.1% (18/588) of smears reported as WNL by cytology and 5.9% (2/34) in smears unsatisfactory for evaluation. Conclusions: The overall percentage of HPV positivity in routine cervical screening samples is comparable with abnormal findings in cytology. Conventional Pap smear 'missed' a few samples. Since HPV testing is expensive, our results may provide valuable information for strategising implementation of effective cervical cancer screening in a country with limited resources like Malaysia. If Pap smear coverage could be improved, HPV testing could be used as an adjunct method on cases with ambiguous diagnoses.

• 대한수의학회지
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• 제36권1호
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• pp.119-129
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• 1996

The present study was conducted to rapidly detect Listeria monocytogenes in raw milk. Specificity and sensitivity of polymerase chain reaction(PCR) technique, and direct PCR were examinded in raw milk, also were compared the calssical culture methods with PCR technique. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L nomocytogenes. In the PCR specificity tests, each of the 10 strains of L monocytogenes tested gave a single 70-bp band. But the other six Listera spp tested gave negative results. Results of the sensitivity tests showed that as few as 2 CFU of L monocytogenes in pure cultures could be detected with 16S rRNA-based primers, L-1 and L-2. In different PCR cycles, a PCR product was detected with $10^3$ cells of L monocytogenes from 25 cycles to 50 cycles and the concentration of PCR products was cycle-dependent. Raw milk samopes added L monocytogenes cells gave negative results. However, these samplers gave a single 70-bp band by pretreatment of pronase, and PCR products were detected with $10^1$ cells of L monocytogenes. To detemine the most sensitive culture protocol to use in conjunction with the PCR assay, raw milk samples were inoculated with L monocytogenes at concentrations ranging from 1 to $5.7{\times}10^4CFU/ml$. PCR assays from Listeria enrichment broth(LEB) containing raw milk samples added L monocytogene EGD could dtect 10 cells in pronase-pretreated samples without incubation, and 1 cell of L monocytogenes in both 12 hr and 24 hr incubation, respectively. Isolation raw of PCR assays was similar to that of classical culture methods, but required time for detection of L monocytogenes could remarkably be reduced compare to culture methods.

• 한국정보과학회논문지:시스템및이론
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• 제30권5_6호
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• pp.280-289
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• 2003

웜홀 네트웍에서 교착상태의 복구를 기반으로 하는 라우팅 알고리즘은 간단한 하드웨어 구조와 높은 라우팅 적응성으로 인해 관심을 이끌었다. 진보적인 교착상태 복구 방안들은 교착상태에 속한 패킷들을 삭제하는 대신에 소수 전용 리소스들을 통해 전송한다. 교착상태에 속한 패킷은 타임아웃에 의해 선정하는데 다양한 트래픽 형태 및 패킷 길이를 고려하여 가장 바람직한 성능을 가져오는 제한 시간 값을 결정하기는 매우 어려운 일이다. 본질적으로, 타임아웃을 사용하는 현재의 방법들은 네트웍 부하가 심하거나 메시지 길이가 긴 경우에 교착상태의 존재를 잘못 판단할 가능성이 크다. 또한 교착상태가 발생할 경우, 하나 이상의 메시지가 교착상태로 판단되어 복구를 위해 마련된 자원을 과포화시킬 수 있다. 본 논문에서는 타임아웃을 사용하지 않고 보다 정확히 교착상태를 발견하는 방안을 제시한다. 제안한 방안은 교착 상태를 잘못 판단하는 확률을 현저히 낮출 수 있다. 또한 순환 구조를 이루는 대기 상태의 메시지들 중에서 하나만을 교착상태라고 선언함으로써 복구에 따른 부담을 감소시킨다.

• 생태와환경
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• 제36권2호통권103호
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• pp.97-107
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• 2003

어류 vitellogenin은 난황전구체로 난형성과정동안에 estradiol의 체내 순환에 의해 암컷의 간에서 생산되며 alkylphenol류와 같은 비이온성 계면활성제 등의 내분비계 장애물질에 의해 수컷에서도 생산된다. 물 환경의주요 생물종인 어류는 내분비계장애물질에 의해 암컷에서는 번식률 저하와 함께 수컷에서는 정소의 축소 및 이에 따른 암컷화가 관찰된다. 특히 수컷에서 내분비계 장애물질에 의해 유도된 vitellogenin의 생성을 이용하여 환경오염에 의해 유도된 생물체의 유전자 발현 변화 뿐만 아니라 이를 토대로 특정지역의 환경오염을 모니터링할 수 있다. 본 논문에서는 어류의 vitetlogenin를 이용하여 수환경의 내분비계장애물질의 검출과 환경오염모니터링을 위한 biomarker로서의 유용성을 검토하였다.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제5권9호
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• pp.275-284
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• 2016

원자력분야에서 사용되는 안전관련 소프트웨어는 계획단계부터 설치단계까지의 전 생명주기 공정을 통해 개발과 확인검증, 안전성 분석, 그리고 품질보증 활동을 수행해 소프트웨어의 안전성을 보장하고 있다. 그러나 이러한 개발과 검증공정을 통한 평가는 시간과 비용을 많이 필요로 한다. 또한, 소프트웨어의 품질을 향상시키기 위해 다양한 활동을 수행했다고 주장하지만, 어느 정도의 품질이 향상되었는지 확인하기에는 한계가 있다. 이러한 한계를 극복하기 위해서 정량적인 평가를 수행할 수 있는 소프트웨어 신뢰도 계산 방법을 제안한다. 특히, 소프트웨어가 사용하는 메모리 공간에 고장을 주입하여 소프트웨어의 고장을 모사하고, 주입된 고장에 가중치를 부여하여 고장 민감도에 차이를 두고, 감지능력을 평가하여 소프트웨어 고장율을 계산한다. 이러한 고장율을 활용하여 소프트웨어 신뢰도 계산을 수행하면 정량적인 평가결과를 획득할 수 있게 된다.

• Structural Monitoring and Maintenance
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• 제2권3호
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• pp.253-267
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• 2015

Corrosion has significant adverse effects on the durability of reinforced concrete (RC) structures, especially those exposed to a marine environment and subjected to mechanical stress, such as bridges, jetties, piers and wharfs. Previous studies have been carried out to investigate the corrosion behaviour of steel rebar in various concrete structures, however, few studies have focused on the corrosion monitoring of RC structures that are subjected to both mechanical stress and environmental effects. This paper presents an exploratory study on the development of corrosion monitoring and detection techniques for RC structures under the combined effects of external loadings and corrosive media. Four RC beams were tested in 3% NaCl solutions under different levels of point loads. Corrosion processes occurring on steel bars under different loads and under alternative wetting - drying cycle conditions were monitored. Electrochemical and microscopic methods were utilised to measure corrosion potentials of steel bars; to monitor galvanic currents flowing between different steel bars in each beam; and to observe corrosion patterns, respectively. The results indicated that steel corrosion in RC beams was affected by local stress. The point load caused the increase of galvanic currents, corrosion rates and corrosion areas. Pitting corrosion was found to be the main form of corrosion on the surface of the steel bars for most of the beams, probably due to the local concentration of chloride ions. In addition, visual observation of the samples confirmed that the localities of corrosion were related to the locations of steel bars in beams. It was also demonstrated that electrochemical devices are useful for the detection of RC beam corrosion.

• Smart Structures and Systems
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• 제6권5_6호
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• pp.423-438
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• 2010

Wireless smart sensors enable new approaches to improve structural health monitoring (SHM) practices through the use of distributed data processing. Such an approach is scalable to the large number of sensor nodes required for high-fidelity modal analysis and damage detection. While much of the technology associated with smart sensors has been available for nearly a decade, there have been limited numbers of fulls-cale implementations due to the lack of critical hardware and software elements. This research develops a flexible wireless smart sensor framework for full-scale, autonomous SHM that integrates the necessary software and hardware while addressing key implementation requirements. The Imote2 smart sensor platform is employed, providing the computation and communication resources that support demanding sensor network applications such as SHM of civil infrastructure. A multi-metric Imote2 sensor board with onboard signal processing specifically designed for SHM applications has been designed and validated. The framework software is based on a service-oriented architecture that is modular, reusable and extensible, thus allowing engineers to more readily realize the potential of smart sensor technology. Flexible network management software combines a sleep/wake cycle for enhanced power efficiency with threshold detection for triggering network wide operations such as synchronized sensing or decentralized modal analysis. The framework developed in this research has been validated on a full-scale a cable-stayed bridge in South Korea.

• 인간식물환경학회지
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• 제21권6호
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• pp.485-492
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• 2018

This study aimed to develop and validate highly sensitive determination method of a prostaglandin ($PGE_1$, OP 1206) in human plasma by LC-MS/MS using column switching. Plasma stored at $-30^{\circ}C$ and treated with methanol effectively inhibited interferences synthesized post-sampling. Samples were added with internal standard and were separated by reversed-phase HPLC with a cycle time of 30min. The method was selective for OP 1206 and the regression models, based on internal standard, were linear across the concentration range 0.5-50 pg/mL with the limit of quantification of 0.5 pg/mL (limit of quantitation, LOQ) for OP 1206. The calibration curve of OP 1206 standards spiked in five individual plasma samples was linear ($r^2=0.9999$). Accuracy and precision at the concentrations of 0.5, 1.5, 5.0 and 40 pg/mL, and at the lower LOQ of 0.5 pg/mL were excellent at 20%. OP120 < 6 was stable in plasma samples for at least 24 hours at room temperature, 24 hours frozen at $-70^{\circ}C$, 24 hours in an auto sampler at $6^{\circ}C$, and for two freeze/unfreezing cycles. The validated determination method successfully quantified the concentrations of OP 1206 in plasma samples from simulated administrating a single $5{\mu}g$ OP 1206 formulation. Thus, this novel LC-MS/MS technique for drug separation, detection and quantitation is expected to become the standard highly-sensitive detection method in bioanalysis and to be applied to many low dose pharmaceutical products.

• 한국미생물·생명공학회지
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• 제49권4호
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• pp.510-518
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• 2021

Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae. N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)-functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 10³ to 10⁹ CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.

• 한국산학기술학회논문지
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• 제18권5호
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• pp.716-722
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• 2017

본 연구에서는 Fire Dynamics Simulation (FDS)를 이용하여 화재 기류 전파 경로 상에 플랜트 설비 유무가 공간 내 열유동 특성에 미치는 영향을 알아보기 위해 화원 위치에 따른 지하 복합 발전 플랜트 내 화재 해석을 수행하였다. 화원의 크기는 10 MW이며, 화원 상부의 장애물(설비)의 유무에 따라 화원 위치가 천장 및 화원 상부에서의 열 기류 선단의 전파 특성을 미치는 영향을 정량적으로 비교분석하였다. 결과로서, 화원 상부에 장애물이 있을 경우, 화재 기류가 화원 상부 천장에 도달하는 시간이 장애물이 없을 때에 비해 약 5 배가량 증가하였다. 화원 상부 천장 벽면의 천장 기류 시작 지점으로부터 거리에 따른 각 지점에서 열 기류 선단의 전파 시간의 평균적으로 장애물이 없는 경우에 비해 약 70% 가량 증가하였으며, 특히 10 m 지점에서는 4 배 가까이 증가하였다. 이는 장애물이 화원으로부터 발생하는 수직 열기류의 흐름을 방해하고, 장애물 뒤 쪽에 불안정한 후류가 형성되었기 때문이다. 따라서 지하복합 발전 플랜트 내 피난 및 재난 관리의 초기 대응 목적의 화재 감지 설비 시스템 설계 시 화재 시나리오에 따른 열유동 분석이 중요할 것으로 판단된다.