• 생약학회지
• /
• 제50권1호
• /
• pp.53-58
• /
• 2019

Morus alba fruit contains several anthocyanins and flavonoids like cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. Extractions from Morus alba fruit were performed with five different compositions of ethanol/water [v/v]. High-Performance Liquid Chromatography (HPLC) was used for the validation and content determination of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. The contents of cyanidin-3-O-rutinoside in ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 1.150%, 0.822%, 0.749%, 0.663% and 0.597%, respectively. Quercetin-3-O-rutinoside contents of ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 0.167%, 0.161%, 0.159%, 0.155% and 0.096%, respectively. The highest contents of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside were in the ethanol extract with 100% ethanol/water [v/v]. These HPLC analysis method could be used as basic data for standardization of functional food from Morus alba fruit.

• 한국식품과학회지
• /
• 제43권2호
• /
• pp.149-155
• /
• 2011

4종류의 다공성 합성흡착제(Sepabeads 207, 700, 850, Diaion HP 20)를 사용하여 Rubus coreanus 과실의 물 추출물로부터 과당 및 비polyphenol성분을 효과적으로 제거하여 DPPH라디칼 소거능 및 화학발광법을 이용한 superoxide 라디칼 소거능이 증가된 농축물을 효율적으로 얻을 수 있었다. 또한, 실시간 HPLC $ABTS^{{\cdot}+}$ 활성분석 시스템을 활용하여 6개의 항산화 능력을 갖는 활성피크를 확인할 수 있었으며 이들 대상으로 LC-MS/MS분석을 실시하여 5개의 anthocyanin인 cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside, pelargonidin-3-rutinoside를 mass pattern 및 분자량 확인 과정을 통해 잠정적 화학구조 동정을 하였다. 수율과 항산화 효능을 고려해 볼 때, 4종의 합성흡착제 중에서 Diaion HP 20이 가장 우수한 결과를 나타내었다. 이처럼 적절한 합성흡착제의 선택으로 표적물질에 대한 polyphenol성 물질의 농축뿐만 아니라 생리학적 활성증대효과를 가져올 수 있어 적절한 합성흡착제의 선택은 식품 및 의약품의 정제 및 분리에 유용하게 활용될 수 있음이 확인되었다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.106-106
• /
• 2018

Cyanidin 3 - rutinoside chloride (CRC) is major anthocyanin, found in Schisandra chinensis, is known to have antioxidant, anticancer, anti-inflammatory, tonic, and anti-aging effects in Korea, China and Japan. In the present study, the human mast cell line (HMC-1) was used to investigate the effects on the production of pro-inflammatory mediators. In this study, CRC showed no cytotoxicity in HMC-1. CRC significantly inhibited the secretion of inflammatory cytokines such as tumor necrosis factor $(TNF)-{\alpha}$ and interleukin (IL)-6 in PMA plus A23187 cacium ionophore (PMACI)-stimulated HMC-1 cells. In addition, CRC suppressed the serum levels of IgE. Furthermore, CRC decreased the PMACI- stimulated phosphorylation of mitogen activated protein kinases (MAPKs) such as p-ERK, p- JNK and p-P38. These results indicate that the pharmacological actions of CRC suggest their potential activity for treatment of allergic inflammation through the down-regulation of mast cell activation.

• Journal of Microbiology and Biotechnology
• /
• 제25권11호
• /
• pp.1842-1848
• /
• 2015

Cyanidin-3-glucoside (C3G) has been known to be more bioavailable than cyanidin-3-rutinoside (C3R), the most abundant anthocyanin in black raspberry (Rubus occidentalis). The aim of this study was to enhance the bioavailability of anthocyanins in black raspberry by cleaving ʟ-rhamnose in C3R using crude enzyme extracts (CEEs) from Aspergillus usamii KCTC 6956, A. awamori KCTC 60380, A. niger KCCM 11724, A. oryzae KCCM 12698, and A. kawachii KCCM 32819. The enzyme activities of the CEEs were determined by a spectrophotometric method using ρ-nitrophenyl-rhamnopyranoside and ρ-nitrophenyl-glucopyranoside. The CEE from A. usamii had the highest α-ʟ-rhamnosidase activity with 2.73 U/ml at 60℃, followed by those from A. awamori and A. niger. When bioconversion of C3R to C3G in black raspberry was analyzed by HPLC-DAD, the CEEs from A. usamii and A. awamori hydrolyzed 95.7% and 95.6% of C3R to C3G, respectively, after 2 h incubation. The CEEs from A. kawachii and A. oryzae did not convert C3R to C3G in black raspberry.

• Natural Product Sciences
• /
• 제21권1호
• /
• pp.20-24
• /
• 2015

Cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside (C3R) were isolated by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (1 : 3 : 1 : 5 : 0.01, v/v) to give pure C3G (34.1 mg) and C3R (14.3 mg) from 1.5 g crude mulberry fruit extract. Using the pure C3G and C3R, a reliable high-performance liquid chromatography (HPLC) method was developed and validated to determine the C3G and C3R contents in mulberry fruit. C3G and C3R were separated simultaneously using an Eclipse XDB-C18 column ($4.6{\times}250mm$ I.D., $5{\mu}m$) coupled with a photodiode array detector (PDA). The gradient elution of the mobile phase consisting of acetonitrile (0.5% formic acid) and water (0.5% formic acid) was applied (1.0 mL/min), and the detection wavelength was 520 nm. The calibration curves of C3G and C3R showed good linearity (both with $r^2=0.9996$) in the concentration range $15.625-500{\mu}g/mL$, and the relative standard deviations (RSD%) of intra- and inter-day variability were in the ranges 2.1 - 8.2% and 4.1 - 17.1%, respectively. The accuracies were ranged 96.5 - 102.6% for C3G and C3R, respectively. The developed HPLC method was used to determine the contents of C3G and C3R in newly harvested mulberry from eight different provinces of Korea.

• 한국식품영양학회지
• /
• 제36권6호
• /
• pp.543-550
• /
• 2023

Mulberry fruit is a superior source of polyphenols, especially anthocyanin, and has a long history of use as an edible fruit and traditional medicine. The anthocyanin composition of mulberry fruit from 15 Korean cultivars was analyzed by ultra-performance liquid chromatography diode array detector with quadrupole time of flight/mass spectrometry (UPLC-DAD-QToF/MS) based on a cyanin internal standard. The four glycosides were identified by comparison with authentic standards and published reports. The major anthocyanin was cyanidin 3-O-glucoside (71.7%), followed by cyanidin 3-O-rutinoside (26.6%). The minor components (total of 1.7%) were pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside. The total anthocyanin content (mg/100 g, dry weight) of mulberry fruit varied by cultivar and ranged from 471.5±4.0 (Su Hong) to 4,700.2±54.0 (Gwa Sang2). Among the 15 cultivars examined, Gwa Sang2 showed the highest level of cyanidin 3-O-glucoside (3,133.4±32.6), which was 9-fold higher than that of Su Hong (351.5±3.4). In conclusion, anthocyanin profiles, including pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside, were reported for the first time from 15 Korean mulberry fruit cultivars. The results will contribute valuable information on pharmaceutical properties, breeding superior mulberry cultivars, and food industries.

• 한국약용작물학회지
• /
• 제20권4호
• /
• pp.259-269
• /
• 2012

This study was performed to determined the antioxidant activities, anticancer and immuno-activities of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Anthocyanin fraction extracted from Bokbunja revealed the presence of three anthocyanin components, which were tentatively identified as cyanidin 3-O-sambubioside, cyanidin 3-O-xylosylrutinoside and cyanidin 3-O-rutinoside using RP-HPLC/DAD/MS. The anthocyanin fraction from Bokbunja always showed reducing power and high scavenging activities against DPPH, hydroxy radical (OH) and superoxide anion radical ($O_2{^-}$) similar to general synthetic antioxidant and polyphenol compounds from plant origin. Anthocyanin fraction from Bokbunja showed high inhibition on proliferation of LNCaP and A549 cells and did not inhibit the proliferation of other cancer cells. Immuno-activities of Anthocyanin fraction from Bokbunja were investigated, it showed high promotion of human B and T cells growth about 50% and secretion of IL-6 and TNF-${\alpha}$ by treatment after 6 days. Over all, the result of the study suggest that anthocyanin fraction from Bokbunja displays antioxidant activity comparable to that general synthetic antioxidant, also, anthocyanin fraction from Bokbunja are expected to be good candidate for development into source of anticaner and immuno-activator agent in food industry.

• 한국식품과학회지
• /
• 제40권1호
• /
• pp.63-69
• /
• 2008

오디 와인의 제조를 위한 최적 발효 조건을 선정하였으며, 발효 중 이화학적 특성 및 anthocyanin 색소의 함량 변화를 조사하였다. 오디 원료의 일반 성분은 수분 83.75%, 조단백질 1.95%, 조지방 0.22%, 회분 0.97%이었으며, pH 4.56, 적정산도 0.50%, 가용성 고형분 13.0 $^{\circ}Brix$, 색도는 명도(L) 8.96, 적색도(a) 6.55, 황색도(b) 4.96으로 나타났다. 주요 유리당으로 fructose와 glucose가 검출되었으며, 주요 유기산으로 citric, malic, succinic acid가 검출되었다. 시험에 사용한 4종 효모 모두 오디 과즙에서 정상적인 알코올 발효를 하였으며, Saccharomyces cerevisiae KCCM 12224로 발효한 오디 와인의 에탄올 생성량과 적색도가 각각 11.0%와 3.60로서 가장 높았다. 가용성 고형분 24 $^{\circ}Brix$와 $26^{\circ}C$에서 발효시 에탄올 생성량, 적정산도 및 적색도 등에서 우수한 특성을 나타냈다. 오디와인의 발효 초기 주요 유리당은 sucrose, fructose 및 glucose 이었으며, 발효기간에 따라 현저히 감소하였다. 유기산 중 citric acid의 함량은 발효 기간 중 비슷하게 유지되었고, malic acid는 감소하였으며, lactic acid와 succinic acid는 증가하였다. 오디 와인의 주요 anthocyanin 색소는 cyanidin-3-glucoside(C-3-G)와 cyanidin-3-rutinoside(C-3-R)가 검출되었으며, C-3-G의 함량은 발효 초기 195.95mg%에서 발효 2일째 15.37 mg%로 급격한 감소를 보였으며, C-3-R은 발효 기간중 점차 감소하여 발효 10일째 45.53 mg%를 나타냈다. 이상의 결과로부터, 오디 와인 제조를 위하여 오디 마쇄액에 $K_2S_2O_5$ 200 ppm을 처리하고, 설탕으로 24 $^{\circ}brix$로 보당한 후 S. cerevisiae KCCM 12224 배양액 3%(v/v)를 접종하여 $26^{\circ}C$에서 8일 동안 발효하는 것이 최적 조건인 것으로 나타났다.

• 한국식품영양과학회지
• /
• 제45권2호
• /
• pp.202-208
• /
• 2016

본 연구에서는 오디 유산균 발효물의 식품산업에서의 다양한 이용 가능성을 확인하기 위해 이화학적 특성 및 항산화 활성을 조사하였다. 생균수는 발효 24시간에서 가장 높은 8.31 log CFU/mL를 나타낸 다음 시간이 지남에 따라 균이 감소하였으며, pH 및 산도 또한 각각 발효 24시간에서 유의적인 감소(pH 3.90) 및 증가(0.15%)를 나타내었다. 색도는 L값 및 a값이 오디 발효 후 감소하고 b값 및 ${\Delta}E$값은 증가하였다. 총 안토시아닌 함량은 오디 발효물에서 100 g당 171.40 mg으로 함량이 증가하였으며, 안토시아닌 조성에서도 cyanidin-3-glucoside 및 cyanidin-3-rutinoside가 각각 100 g당 61.39 mg 및 85.45 mg으로 증가하였다. 오디 발효물의 총 폴리페놀 및 총 플라보노이드 함량은 100 g당 10.75 g 및 5.02 g으로 발효 전에 비해 높게 나타났으며, ORAC 또한 발효물에서 $292.94{\mu}M/g$으로 활성이 증가하였다. DPPH 및 superoxide radical 소거 활성에서는 오디 발효물에서 각각 17.40~58.21%, 32.63~87.34%로 무발효물보다 높은 소거 활성을 나타내었다. FRAP 및 환원력에서도 오디 발효물의 활성이 $37.03{\sim}762.13{\mu}M$ 및 0.12~0.74를 나타내어 농도 의존적으로 항산화 활성이 증가하였다. 따라서 페놀성 화합물 및 안토시아닌 함량이 증가한 오디 유산균 발효물을 향후 기능성 식품 소재로 적용 가능할 것으로 사료된다.

• 동아시아식생활학회지
• /
• 제23권6호
• /
• pp.768-777
• /
• 2013

Physicochemical characteristics and analysis of functional constituents of mulberry (Morus alba L.) fruit juices were investigated according to four different mulberry cultivars, including iksuppong, daeseongppong, cheongilppong and kwasangppong. Among the four mulberry cultivars examined, the small-sized cheongilppong had higher soluble solid content and lower titratable acidity, whereas the big-sized daeseongppong had higher titratable acidity and lower soluble solid content than other mulberry cultivars. Cheongilppong had higher contents of fructose and glucose, while daeseongppong had higher contents of citric and malic acids than the other mulberry cultivars. Kwasangppong had higher contents of two anthocyanins, cyanidin 3-glucoside and cyanidin 3-rutinoside, while cheongilppong had lower contents of two anthocyanins than the other mulberry cultivars. Daeseongppong had higher contents of resveratrols, flavonoids and moracin, whereas cheongilppong had higher levels of protocatechuic, chlorogenic and caffeic acids than the other mulberry fruits. Of the four mulberry cultivars, daeseongppong and cheongilppong had higher contents of GABA and DNJ, respectively, than the other mulberry cultivars. These results provide useful information to food technologists for the development and standardization of high quality mulberry juices as well as their processed foods.