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Quantitative Analysis of Cyanidin-3-O-rutinoside and Quercetin-3-O-rutinoside from Extracts of Morus alba Fruit  

Kim, Min-Ji (College of Pharmacy, Kyungsung University)
Lee, Ji Hyun (College of Pharmacy, Kyungsung University)
Pyo, Jae Sung (College of Pharmacy, Kyungsung University)
Kim, Hye Kyung (College of Pharmacy, Kyungsung University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.1, 2019 , pp. 53-58 More about this Journal
Abstract
Morus alba fruit contains several anthocyanins and flavonoids like cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. Extractions from Morus alba fruit were performed with five different compositions of ethanol/water [v/v]. High-Performance Liquid Chromatography (HPLC) was used for the validation and content determination of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside. The contents of cyanidin-3-O-rutinoside in ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 1.150%, 0.822%, 0.749%, 0.663% and 0.597%, respectively. Quercetin-3-O-rutinoside contents of ethanol extracts (100%, 70%, 40%, 20% and 0%, ethanol/water [v/v]) were 0.167%, 0.161%, 0.159%, 0.155% and 0.096%, respectively. The highest contents of cyanidin-3-O-rutinoside and quercetin-3-O-rutinoside were in the ethanol extract with 100% ethanol/water [v/v]. These HPLC analysis method could be used as basic data for standardization of functional food from Morus alba fruit.
Keywords
Morus alba fruit; Cyanidin-3-O-rutinoside; Quercetin-3-O-rutinoside; Quantitative analysis; HPLC; Validation;
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