• Title/Summary/Keyword: Cy5

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Fabrication of Poly(diallyldimethylammonium chloride) - Patterned Substrates for Patterning of Single Strand DNA Using Ion Implantation

  • Ahn, Mi-Young;Hwang, In-Tae;Jung, Chan-Hee;Choi, Jae-Hak;Nho, Young-Chang
    • Journal of Radiation Industry
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    • v.5 no.3
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    • pp.243-247
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    • 2011
  • In this study, a convenient method for the selective immobilization of single strand DNA (ssDNA) on a polymer surface was described. A positively charged polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA), was spin-coated on a tissue culture petridish and the micropatterns of the PDDA were formed by selective ion implantation through a pattern mask. The surface property of the implanted PDDA was investigated by using a surface profiler and FT-IR spectrometer. Cy3-labeled ssDNA was selectively immobilized on the PDDA patterns through ionic interaction and thus, well-defined ssDNA patterns were obtained.

On the Analysis of Container Physical Distribution System in Pusan Port (부산항 컨테이너 물류 시스템 분석에 관하여)

  • Park, Chang-Ho;Lee, Cheol-Yeong
    • Journal of Korean Port Research
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    • v.5 no.1
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    • pp.55-69
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    • 1991
  • A Container terminal is a physical link between the ocean and land modes of transport and a major component of the container physical distribution system. A number of projects to increase terminal productivity in the world were found to be tied directly to increasing the efficiency of the intermodal activities, that is, to improving the physical distribution process. This is an indication that the productivity of container terminal is being considered withing a system prospective. The purpose of this study is to establish a model of the container physical distribution system for Pusan port linked with 4 sub-systems including Navigational aids system, Cargo handling/transfer/storage system on dock, Off-dock CY system and In-land transport connection system. and to analyze the system. From this analysis, we found that the system had three bottlenecks on the container physical distribution process in Pusan port : a) cargo handling b) storage and c) inland transport, and showed a way to improve the system.

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Economic Analysis on Inter-Connected System of Port Transport and In-land Transportation -A Container Cargo Transport Oriented- (부산항 항만 운송과 육상 교통 연계시스템에 관한 경제성 분석 -컨테이너 화물 운송을 중심으로-)

  • Kwak, Kyu-Seok;Kim, Tae-Gon;Choi, Jae-Soo;Ahn, Ki-Myung
    • Journal of Korean Port Research
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    • v.5 no.1
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    • pp.29-54
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    • 1991
  • In this study, to identify the ineffectiveness of inter-connected system of cargo volume between the Pusan Port and inland areas and also to make more rational suggestions, the following conclusions were drawn by analyzing Container Cargo Traffic from BCTOC to Off-Dock CY: 1. There existed about 30% to 50% reduction in the container transport times when the container transport vehicles were operated during the off-peak period to alleviate the traffic congestion due to mixed traffic. 2. There appeared to be more economic when Off-Dock DY's scattered around the City of Pusan were unified in one ODCY Unit at YangSan, and the Exclusive Overpass Freeway System for the container transport vehicles were constructed and operated on the existing Urban Freeway from BCTOC ti this ODCY Unit (Expected to make about 230 billions Won in net present value by NPV method).

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Effects of Continuous Application of Green Manures on Microbial Community in Paddy Soil

  • Kim, Sook-Jin;Kim, Kwang Seop;Choi, Jong-Seo;Kim, Min-Tae;Lee, Yong Bok;Park, Ki-Do;Hur, Seonggi
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.528-534
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    • 2015
  • Green manure crops have been well recognized as the alternative for chemical fertilizer, especially N fertilizer, because of its positive effect on soil and the environment. Hairy vetch and green barley are the most popular crops for cultivation of rice in paddy field. This study was conducted to evaluate effects of hairy vetch and green barley on soil microbial community and chemical properties during short-term application (three years). For this study, treatments were composed of hairy vetch (Hv), green barley (Gb), hairy vetch + green barley (Hv+Gb), and chemical fertilizer without green manure crops (Con.). Hv+Gb treatment showed the highest microbial biomass among treatments. Principal component analysis (PCA) showed that PC1 (73.0 %) was affected by microbial biomass and PC2 (21.5 %) was affected by fungi, cy19:0/18:$1{\omega}7c$ (stress indicator). Combined treatment with hairy vetch and green barley could be more efficient than green manure crop treatment as well as chemical fertilizer treatment for improvement of soil microorganisms.

Cloning, Expression, and Characterization of a Novel GH-16 β-Agarase from Agarivorans sp. JA-1 (Agarivorans sp. JA-1 유래 신규 GH-16 β-agarase의 클로닝, 발현 및 특성)

  • Jeon, Myong Je;Kim, A-Ram;Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.22 no.11
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    • pp.1545-1551
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    • 2012
  • Authors report the glycoside hydrolase (GH) family 16 ${\beta}$-agarase from the strain of Agarivorans sp. JA-1, which authors previously stated as recombinant expression and characterization of GH-50 and GH-118 ${\beta}$-agarase. It comprised an open reading frame of 1,362 base pairs, which encodes a protein of 49,830 daltons consisting of 453 amino acid residues. Valuation of the total sequence showed that the enzyme has 98% nucleotide and 99% amino acid sequence similarities to those of GH-16 ${\beta}$-agarase from Pseudoalteromonas sp. CY24. The gene corresponding to a mature protein of 429 amino acids was recombinantly expressed in Escherichia coli, and the enzyme was purified to homogeneity by affinity chromatography. It showed maximal activity at $40^{\circ}C$ and pH 5.0, representing 67.6 units/mg. Thin layer chromatography revealed that mainly neoagarohexaose and neoagarotetraose were produced from agarose. The enzyme would be valuable for the industrial production of functional neoagarooligosaccharides.

Comparison of Microbial Community of Orchard Soils in Gyeongnam Province (경남지역 과수원 토양 미생물 군집 비교)

  • Lee, Young-Han;Lee, Seong-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.492-497
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    • 2011
  • Soil management for orchard depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 orchard in Gyeongnam Province by fatty acid methyl ester (FAME) method. The average concentrations in the orchard soils were $332nmol\;g^{-1}$ of total FAMEs, $94nmol\;g^{-1}$ of bacteria, $46nmol\;g^{-1}$ of Gram-negative bacteria, $42nmol\;g^{-1}$ of Gram-positive bacteria, $4.8nmol\;g^{-1}$ of actinomycetes, $54nmol\;g^{-1}$ of fungi, and $9.1nmol\;g^{-1}$ of arbuscular mycorrhizal fungi. In addition, sandy loam soils had significantly low ratio of cy19:0 to 18:$1{\omega}7c$ compared with that of loam soils (p<0.05), indicating that microbial stress decreased. The average soil microbial communities in the orchard soils were 28.1% of bacteria, 15.9% of fungi, 13.6% of Gram-negative bacteria, 12.5% of Gram-positive bacteria, 2.8% of arbuscular mycorrhizal fungi, and 1.4% of actinomycetes. The soil microbial community of Gram-negative bacteria in peach cultivating soils was significantly higher than that of pear cultivating soils (p<0.05).

Pulsed Photostimulated Luminescence of Irradiated Black and White Peppers and Effects of Long-Term Storage

  • Oh, Man-Jin;Yi, Sang-Duk;Jeoung, Hyun-Kyo;Chang, Kyu-Seob;Yang, Jae-Seung;Song, Chi-Kwang
    • Preventive Nutrition and Food Science
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    • v.7 no.2
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    • pp.195-200
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    • 2002
  • Changes in accumulated pulsed photostimulated luminescene (PPSL) signals were observed after storage, which affected the ability to detect irradiation in black and white peppers. The PPSL curves were accumulated linearly during the 120 s measurement times, and PPSL signals increased according to irradiation doses. Threshold levels of black and white peppers were below 557$\pm$220 and 503$\pm$92 photon counts in 60 s, and below 679$\pm$351 and 812 $\pm$ 648 photon counts in 120 s, respectively. The PPSL signals of black and white peppers linearly increased with irradiation dose up to 5 kGy, but very little from 5~10 kCy. The accumulated PPSL signals of irradiated black and white peppers had higher decay rates when stored in normal room conditions than in a darkroom Detection of irradiation was possible for up to 12 months after irradiation, if the samples were stored in a darkroom.

Effects of Immunoactivity on Ascaris suum Infection in Mice (마우스에 있어서 멱역활성이 돼지회충의 감염에 미치는 영향)

  • Lee, Jae-Gu;Park, Bae-Geun;Seo, Yeong-Seok
    • Parasites, Hosts and Diseases
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    • v.29 no.3
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    • pp.279-292
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    • 1991
  • The immune response to sheep red blood cell (sRBC) was monitored in the mice infected with Ascaris strum or Trichinella spiralis. The effects of the infection with T. spiralis or the injection with cyclophosphamide (CY) as an immunosuppression agent prior to challenge infection with the embryonated eggs of A. suum were monitored in mice by means of the level of infection with A. strum and cellular and humoral immune response to sRBC. following the oral administration of 1, 000 eggs of A. suum to mice, delayed-type hypersensitivity (DTH) and rosette-forming rate were gradually decreased and reached to the lowest levels at the 5th week and 6th week postinfection, respectively, and then returned to normal at the loth week. The hemagglutinin (HA) and hemolysin (HE) titers were gradually elevated and reached to peak at the 3rd week postinfection, and then returned to normal level. The appearance ratios of the eosinophils and mast cells were in peak at the 4th week and the 2nd week postinfection, respectively. Meanwhile the harvest ratio of A. suum larvae from the liver and lungs was 21.97% at the 1st week postinfection. Following the oral administration of 300 T. spiralis infective larvae, DTH and rosette-forming rate were gradually decreased with the lapse of time and reached the lowest values in the 30th and 21st day of postinfection, and then slightly increased and transiently decreased in the 70th and 80th day of postinfection, respectively. HA and HE titers were the lowest in the 21st and 90th day, whereas the ratios of eosinophils and mast cells were the highest on the 40th and 14th day posti nfecti on, ruts petit i vela. Following the intraperitoneal injection of CY, the body weight, the spleen weight, DTH, rosette-orming ratio, HA and HE titers, the number of WBC and the ratio of the mast cell were predominantly decreased in the 5th day, and then returned to the same value of the 1st day postinjection. The ratio of eosinophils was gradually decreased following to advance of days. At the 1st, 5th and loth days after intraperitoneal injection of CY of 400 mg/kg, a dose with 1, 000 eggs of A. suum was administered orally to mice, and harvest rate of the larvae at the 7th day postadministration was 7.07% in the 1st day, 14.94% in the 5th day, 10.1% in the loth day, 8.02% in control group. The effect of prior infection with infective larvae of T. spiralis upon immunological sequelae of a challenge infection of mice with embryonated eggs of A. suum in 30 or 70 days interval was checked. On the 37th day of prior infection with T. spiralis, that was the 7th day with A. suum postinfection, DTH and rosette-forming rate were drastically decreased, but the ratio of mast cells was highly increased and the ratio of eosinophils, HA and HE titers were fairly increased. On the other hand, the rate of larvae harvest was 9.3% in experimental group in contrast with 22.18% in control group. Meanwhile the effect of immune response to sRBC was similar to that of the former, but DTH and rosettt-forming rate were greatly decreased in the 77th day after prior infection with the 7th day after challenge infection in compariton with control. At that time, Ascaris larvae were harvested 8.3% in experimental group in comparison with 10.5% in control group.

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Interseeding Substitution Ratio Assessment by Using of Molecular Tool (분자생물학적 기법을 이용한 Interseeding률 평가)

  • Jung, Seung-Ho;Lim, Deuk-Jong;Jang, Duk-Hwan
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.131-137
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    • 2010
  • Recently, many domestic golf course have showed interest in introducing new cultivars into existing putting greens without reconstruction. But, any report and survey method for interseeding substitute ratio haven't been published. Therefore, this study was conducted to evaluate the effectiveness for interseeding by investigating how many interseeding plants were introduced into existing putting green by using molecular biologic tool. The Woo Jung Hills Country Club was one time introduced penn A4 into penncross putting green. The Lake Side Country Club was three times introduced CY-2 into penncross putting green. The interseeding substitute ratio using SCAR markers was 20% in the Woo Jung Hills Country Club and 35.5% in Lake Side Country Club. The interseeding putting green in Lake Side Country Club was investigated to three site, strong pressure, middle pressure and weak pressure. These substitute ratios were strong pressure 27.1%, middle pressure 37.3%, and weak pressure 47.2%. The data indicated that an interseeding substitute ratio was more increased three times than one time to introducing new cultivar into existing putting green. A long-term program should be made in order to exchange existing cultivar in putting green without reconstruction.

Gene Expression Profiling of Human Salivary Gland Carcinogenesis with cDNA Microarray (인간 타액선 암발생에서 cDNA Microarray를 이용한 유전자발현 Profile연구)

  • Kim, Eun-Cheol;Shin, Min;Lee, Dong-Geun;Lee, Ju-Seok;Park, Myung-Hee
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.23 no.4
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    • pp.306-323
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    • 2001
  • 종양발생에서 유전자 발현을 확인하고 profile 변화를 monitor하는 것은 병리학적 변화의 원인뿐 아니라 질병탐지와 진료의 새로운 목표를 확인하기 위한 새로운 기회를 제공해준다. cDNA microarray는 수천개의 유전자 발현을 동시에 연구할 수 있는 최신의 방법으로 피부, 유방, 간을 비롯한 다른 인체장기에서는 일부 이루어졌으나 array를 이용해 타액선 종양 연구에서는 전혀 이루어지지 않았다. 인간의 타액선 세포의 악성형질전환을 조절하는 분자적 상태를 연구하기 위해 본 연구는 약 2,000개의 유전자가 print된 cDNA microarray를 이용하여 인간 타액선 도관상피세포주(HSG)와 악하선에서 기원한 미분화 선암종(SGT)간에 비교연구를 하였다. Cy3와 Cy5 dye로 각각의 세포주에서 얻은 RNA와 reciprocal hybridize시키고 GenePix 4000 scanner로 스캔하고 GenePix Pro로 분석한 후 log2로 평균발현비율을 전환시켜 최소 2배이상의 발현을 보이는 유전자를 분석대상으로 하였다. 90%이상의 유전자가 비슷한 발현을 보였으며 2배이상의 발현을 보이는 경우 HSG가 SGT에 비해 72개 유전자가, SGT가 HSG에 비해 111개의 유전자 발현이 up-regulation되어 총 10%미만의 발현차이를 보였고 반복된 hybridization 으로부터 얻은 선택된 spot의 Pearson 상관계수는 -0.85이였다. HSG에서는 6번 p 염색체에서 과발현되는 유전자가 가장 많았고, SGT에서는 11번 q 염색체에서 가장 많았는데 HSG에서는 SGT에 비해 9, 13, 17, 18, 20, 21, 22염색체에서 과발현 되는 유전자 수가 많았고, SGT에서는 HSG에 비해 2, 7, 10, 15 염색체에서 유전자 발현 증가가 관찰되었다. HSG와 SGT간의 유전 발현을 기능별로 분석한 결과 몇 가지 주요 경로가 세포악성에 관련됨을 발견하였고, 타액선 도관상피세포에서 선암종을 구별하는데 기여하는 관련된 몇종의 과다 발현된 유전자를 찾았는데 전사인자, 성장인자 및 수용기, 세포골격 및 세포외기질 단백, 세포내 신호전달조절자 및 인자, 세포표면 항원등의 그룹으로 분류할 수 있었다. 따라서 이러한 microarray를 이용한 분자학적 표지자 연구가 악성 타액선 종양 발생과정에서 큰 도움을 줄 수 있을 뿐 아니라 유전자 조절에 의한 진단, 예후, 치료에서의 정확성을 개선시킬 수 있으리라 여겨진다.

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