• Molecules and Cells
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• 제42권5호
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• pp.418-425
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• 2019

Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.

• Journal of Astronomy and Space Sciences
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• 제39권4호
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• pp.159-167
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• 2022

Because of the small number of spacecraft available in the Earth's magnetosphere at any given time, it is not possible to obtain direct measurements of the fundamental quantities, such as the magnetic field and plasma density, with a spatial coverage necessary for studying, global magnetospheric phenomena. In such cases, empirical as well as physics-based models are proven to be extremely valuable. This requires not only having high fidelity and high accuracy models, but also knowing the weakness and strength of such models. In this study, we assess the accuracy of the widely used Tsyganenko magnetic field models, T96, T01, and T04, by comparing the calculated magnetic field with the ones measured in-situ by the GOES satellites during geomagnetically disturbed times. We first set the baseline accuracy of the models from a data-model comparison during the intervals of geomagnetically quiet times. During quiet times, we find that all three models exhibit a systematic error of about 10% in the magnetic field magnitude, while the error in the field vector direction is on average less than 1%. We then assess the model accuracy by a data-model comparison during twelve geomagnetic storm events. We find that the errors in both the magnitude and the direction are well maintained at the quiet-time level throughout the storm phase, except during the main phase of the storms in which the largest error can reach 15% on average, and exceed well over 70% in the worst case. Interestingly, the largest error occurs not at the Dst minimum but 2-3 hours before the minimum. Finally, the T96 model has consistently underperformed compared to the other models, likely due to the lack of computation for the effects of ring current. However, the T96 and T01 models are accurate enough for most of the time except for highly disturbed periods.

• Smart Structures and Systems
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• 제26권6호
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• pp.693-701
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• 2020

The conventional Kalman Filter (KF) provides a promising way for structural state estimation. However, the physical parameters of structural systems or models should be available for the estimation. Moreover, it is not applicable when the loadings applied to the structures are unknown. To circumvent the aforementioned limitations, a two-stage KF with unknown input approach is proposed for the simultaneous identification of structural parameters and unknown loadings. In stage 1, a modified observation equation is employed. The structural state vector is estimated by KF on the basis of structural parameters identified at the previous time-step. Then, the unknown input is identified by Least Squares Estimation (LSE). In stage 2, based on the concept of sensitivity matrix, the structural parameters are updated at the current time-step by using the estimated structural states obtained from stage 1. The effectiveness of the proposed approach is numerically validated via a five-story shearing model under random and earthquake excitations. Shaking table tests on a five-story structure are also employed to demonstrate the performance of the proposed approach. It is demonstrated from numerical and experimental results that the proposed approach can be used for the identification of parameters of structure and the external force applied to it with acceptable accuracy.

• 전기전자학회논문지
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• 제26권4호
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• pp.596-601
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• 2022

최근 친환경 선박 전환 추세에 따라 EGR 시스템 적용이 증가되고 있다. EGR 시스템의 주요 부품 중 하나인 EGR 블로워는 공력시스템과 이를 구동하는 e-모터 및 인버터 등으로 구성되며, 에너지밀도가 높고 효율 특성이 우수한 영구자석형 동기전동기가 적용되고 있다. 한편, EGR 블로워용 모터의 경우 국내 중소조선기자재 기업들의 개발이 활발하지만, 선박에 탑재되는 인버터는 까다로운 선급인증을 획득해야 되므로 주로 글로벌 선진기업에서 개발한 범용 인버터가 사용되고 있다. 범용 인버터는 자가 튜닝 등 편리한 구동 기능을 갖고 있지만, 최적 제어 실패 시, 사용자 입장에서 원인을 분석하기 어려운 단점이 있다. 본 연구에서는 EGR 블로워용 SPMSM의 최적제어를 위한 전류 벡터 제어 및 Tracking observer를 설계 및 검증하여 범용 인버터의 최적제어 실패원인을 분석하고자 한다.

• 인터넷정보학회논문지
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• 제24권1호
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• pp.39-47
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• 2023

The development of autonomous driving and Advanced Driver Assistance System (ADAS) technology has grown rapidly in recent years. As most traffic accidents occur due to human error, self-driving vehicles can drastically reduce the number of accidents and crashes that occur on the roads today. Obviously, technical advancements in autonomous driving can lead to improved public driving safety. However, due to the current limitations in technology and lack of public trust in self-driving cars (and drones), the actual use of Autonomous Vehicles (AVs) is still significantly low. According to prior studies, people's acceptance of an AV is mainly determined by trust. It is proven that people still feel much more comfortable in personalized ADAS, designed with the way people drive. Based on such needs, a new attempt for a customized ADAS considering each driver's driving style is proposed in this paper. Each driver's behavior is divided into two categories: assertive and defensive. In this paper, a novel customized ADAS algorithm with high classification accuracy is designed, which divides each driver based on their driving style. Each driver's driving data is collected and simulated using CARLA, which is an open-source autonomous driving simulator. In addition, Long Short-Term Memory (LSTM) and Gated Recurrent Unit (GRU) machine learning algorithms are used to optimize the ADAS parameters. The proposed scheme results in a high classification accuracy of time series driving data. Furthermore, among the vast amount of CARLA-based feature data extracted from the drivers, distinguishable driving features are collected selectively using Support Vector Machine (SVM) technology by comparing the amount of influence on the classification of the two categories. Therefore, by extracting distinguishable features and eliminating outliers using SVM, the classification accuracy is significantly improved. Based on this classification, the ADAS sensors can be made more sensitive for the case of assertive drivers, enabling more advanced driving safety support. The proposed technology of this paper is especially important because currently, the state-of-the-art level of autonomous driving is at level 3 (based on the SAE International driving automation standards), which requires advanced functions that can assist drivers using ADAS technology.

• Current Optics and Photonics
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• 제7권3호
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• pp.237-247
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• 2023

In middle- and long-distance imaging systems, due to the atmospheric turbulence caused by temperature, wind speed, humidity, and so on, light waves propagating in the air are distorted, resulting in image-quality degradation such as geometric deformation and fuzziness. In remote sensing, astronomical observation, and traffic monitoring, image information loss due to degradation causes huge losses, so effective restoration of degraded images is very important. To restore images degraded by atmospheric turbulence, an image-restoration method based on improved compound multibranch feature fusion (CMFNetPro) was proposed. Based on the CMFNet network, an efficient channel-attention mechanism was used to replace the channel-attention mechanism to improve image quality and network efficiency. In the experiment, two-dimensional random distortion vector fields were used to construct two turbulent datasets with different degrees of distortion, based on the Google Landmarks Dataset v2 dataset. The experimental results showed that compared to the CMFNet, DeblurGAN-v2, and MIMO-UNet models, the proposed CMFNetPro network achieves better performance in both quality and training cost of turbulent-image restoration. In the mixed training, CMFNetPro was 1.2391 dB (weak turbulence), 0.8602 dB (strong turbulence) respectively higher in terms of peak signal-to-noise ratio and 0.0015 (weak turbulence), 0.0136 (strong turbulence) respectively higher in terms of structure similarity compared to CMFNet. CMFNetPro was 14.4 hours faster compared to the CMFNet. This provides a feasible scheme for turbulent-image restoration based on deep learning.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2022년도 춘계학술대회
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• pp.413-415
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• 2022

인간의 관절은 인간의 신체를 구성하는 요소로 인간의 행동을 분석하는데 유용한 정보로 활용될 수 있기 때문에 관절 정보를 이용한 행동인식에 대한 많은 연구가 진행되었다. 하지만 각각의 독립적인 관절 정보만을 이용해서 시시각각 변화하는 인간의 행동을 인식하는 것은 매우 복잡한 문제이다. 따라서 학습에 사용할 부가적인 정보 추출 방법과 과거의 상태를 기반으로 현재 상태를 판단하는 고려하는 알고리즘이 필요하다. 본 논문에서는 연결된 관절들의 위치 관계와 각 관절의 위치가 시간의 흐름에 따라 변화하는 것을 고려한 행동 인식 기법을 제안한다. 사전 학습된 관절 추출 모델을 이용하여 각 관절의 위치 정보를 획득하고 연결된 관절 사이의 차 벡터를 이용하여 뼈대 정보를 추출한다. 그리고 두 가지 형태의 입력에 맞춰 간소화된 신경망을 구성하고 LSTM을 더하여 시·공간적 특징을 추출하도록 한다. 9개의 행동으로 구성된 데이터 셋을 이용하여 실험한 결과 각 관절 및 뼈대의 시·공간적 관계 특징을 고려하여 행동 인식 정확도를 측정하였을 때 단일 관절 정보만을 이용한 결과에 비해 뛰어난 성능을 보임을 확인하였다.

• International Journal of Computer Science & Network Security
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• 제23권3호
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• pp.67-80
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• 2023

Rice is an important food crop for most of the population in Nowadays, psychologists consider social media an important tool to examine mental disorders. Among these disorders, depression is one of the most common yet least cured disease Since abundant of writers having extensive followers express their feelings on social media and depression is significantly increasing, thus, exploring the literary text shared on social media may provide multidimensional features of depressive behaviors: (1) Background: Several studies observed that depressive data contains certain language styles and self-expressing pronouns, but current study provides the evidence that posts appearing with self-expressing pronouns and depressive language styles contain high emotional temperatures. Therefore, the main objective of this study is to examine the literary cyber writers' posts for discovering the symptomatic signs of depression. For this purpose, our research emphases on extracting the data from writers' public social media pages, blogs, and communities; (3) Results: To examine the emotional temperatures and sentences usage between depressive and not depressive groups, we employed the SentiStrength algorithm as a psycholinguistic method, TF-IDF and N-Gram for ranked phrases extraction, and Latent Dirichlet Allocation for topic modelling of the extracted phrases. The results unearth the strong connection between depression and negative emotional temperatures in writer's posts. Moreover, we used Naïve Bayes, Support Vector Machines, Random Forest, and Decision Tree algorithms to validate the classification of depressive and not depressive in terms of sentences, phrases and topics. The results reveal that comparing with others, Support Vectors Machines algorithm validates the classification while attaining highest 79% f-score; (4) Conclusions: Experimental results show that the proposed system outperformed for detection of depression trends in literary cyber writers using sentiment analysis.

• 국제학술발표논문집
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.304-311
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• 2022

Construction inspection is a crucial stage that ensures that all contractual requirements of a construction project are verified. The construction inspection capabilities among state highway agencies have been greatly affected due to budget reduction. As a result, efficient inspection practices such as risk-based inspection are required to optimize the use of limited resources without compromising inspection quality. Automated prioritization of textual requirements according to their criticality would be extremely helpful since contractual requirements are typically presented in an unstructured natural language in voluminous text documents. The current study introduces a novel model for predicting the risk level of requirements using machine learning (ML) algorithms. The ML algorithms tested in this study included naïve Bayes, support vector machines, logistic regression, and random forest. The training data includes sequences of requirement texts which were labeled with risk levels (such as very low, low, medium, high, very high) using the fuzzy logic systems. The fuzzy model treats the three risk factors (severity, probability, detectability) as fuzzy input variables, and implements the fuzzy inference rules to determine the labels of requirements. The performance of the model was examined on labeled dataset created by fuzzy inference rules and three different membership functions. The developed requirement risk prediction model yielded a precision, recall, and f-score of 78.18%, 77.75%, and 75.82%, respectively. The proposed model is expected to provide construction inspectors with a means for the automated prioritization of voluminous requirements by their importance, thus help to maximize the effectiveness of inspection activities under resource constraints.

• 국제학술발표논문집
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• The 8th International Conference on Construction Engineering and Project Management
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• pp.463-481
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• 2020

Cutter suction dredgers (CSDs) are widely used in various dredging constructions such as channel excavation, wharf construction, and reef construction. During a CSD construction, the main operation is to control the swing speed of cutter to keep the slurry concentration in a proper range. However, the slurry concentration cannot be monitored in real-time, i.e., there is a "time-lag effect" in the log of slurry concentration, making it difficult for operators to make the optimal decision on controlling. Concerning this issue, a solution scheme that using real-time monitored indicators to predict current slurry concentration is proposed in this research. The characteristics of the CSD monitoring data are first studied, and a set of preprocessing methods are presented. Then we put forward the concept of "index class" to select the important indices. Finally, an ensemble learning algorithm is set up to fit the relationship between the slurry concentration and the indices of the index classes. In the experiment, log data over seven days of a practical dredging construction is collected. For comparison, the Deep Neural Network (DNN), Long Short Time Memory (LSTM), Support Vector Machine (SVM), Random Forest (RF), Gradient Boosting Decision Tree (GBDT), and the Bayesian Ridge algorithm are tried. The results show that our method has the best performance with an R² of 0.886 and a mean square error (MSE) of 5.538. This research provides an effective way for real-time predicting the slurry concentration of CSDs and can help to improve the stationarity and production efficiency of dredging construction.