• Research in Plant Disease
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• v.21 no.3
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• pp.180-185
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• 2015

In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

• Journal of the Korean Society of Clothing and Textiles
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• v.16 no.3 s.43
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• pp.243-250
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• 1992

The purposes of this research were to analyze factor structure and the face image to hairstyles which was made by Computer graphic simulation. To select ten hairstyle, a standard face selected between women of $20\~25$ years, and four factor (straight, curl, hair length, front hair, part hair) which were made of stand of hairstyle were applied. The results were as follows; 1 face image factor by hairstyle variation were found to five factor; negative and pogitive, indivisuality, youthfulness, unbanity, intelligence. 2. The result of analysis to face image by hairstyle factor were (1) In the hair state, straight hair was explained by the youthful, pure, decent image than curl. (2) In the hair length, the longer hair was explained by the more feminine, softness image. The shorter hair was explained by the more vigorous, youthful image. (3) In the presence of front hair, bang hair was explained by the commonness, moderate, classical image than all back hair (4) In the part hair, part hair was explained by the modern and unbanity image than no part hair.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.37-44
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• 2006

The purposes of this study were to analyze and compare EMG activities of the pectoralis major, biceps brachii, triceps brachii, and brachioradialis muscles during biceps curls using a VRT device and an elastic tubing. Fifteen male college students were recruited as subjects and they performed 10-RM and 20-RM biceps curls. For each load and device condition, the mean and peak normalized EMG levels during different phases of a biceps curl were computed. For each load and phase, paired t-test (p.05) was used to find the significant difference between two devices. ANOVA with repeated measures was also used to find the significant difference among phases in terms of EMG values for each muscle. For each load and device condition, the peak and mean EMG levels during different phases of a biceps curl were computed The significant differences between devices were found in biceps brachii for EA, MD, LD phases, and triceps brachii muscles for all phases, respectively. However, no differences were found among phases for any muscle. This indicated that elastic band could have a similar characteristics of VRT. High antagonistic muscle activity as a function of injury prevention which found particularly in VRT device may suggest that elastic tubing can be a safer training device than VRT. This also imply that elastic tubing could be very effective as a home exercise tool for rehabilitation patients and elderly people.

• Journal of Korean Biological Nursing Science
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• v.12 no.3
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• pp.157-165
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• 2010

Purpose: The purpose of this study was to examine the effects of recreation combined exercise program on body composition, physical fitness and depression in elderly women in rural district over a three-month follow-up period. Methods: Recreation combined exercise program (RCEP) was composed of resistance exercise, walking and recreation activity twice a week. The subjects were 52 divided in to experimental group (n=28) and control group (n=24). Arm curl, chair stand, 2 min step test, back scratch, chair sit and reach were measured as pre- or post-test data. Also, Depression was expressed using geriatric depression scale as pre- or post-test data. $X^2$ test, paired t-test and t-test were performed using SAS program. Results: Arm curl (p=.023), 2 minute step test (p<.001), back scratch (p=.004) and chair sit and reach (p=.024) showed the significant difference in the comparison between groups. Depression showed the significant difference in the comparison between groups (p=.008). Conclusion: These findings indicated the RCEP has positive influence upon increasing the physical fitness and improving the depression level. In conclusion, the regular RCEP will contribute to the improving physical and psychologic aspect in elderly women over the long period.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.38-42
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• 2018

Demand for tomatoes has been increasing every year as people desire more healthy food. In Korea, tomatoes are mainly grown in the Chungnam, Chunnam and Kyungnam provinces. Recently, reports of whitefly-transmitted viral diseases have increased due to newly emerging whitefly pressures caused by climate change in Korea. Specifically, in 2017, the main tomato growing areas, Buyeo and Nonsan in Chungnam, showed damage typical of viral infection; therefore, we investigated viral diseases in these areas. We collected samples with virus-like symptoms and found that not only whitefly transmitted Tomato yellow leaf curl virus (TYLCV) and Tomato chlorosis virus (ToCV) were detected but also Tomato mosaic virus (ToMV, for which no specific vector is known) and Tomato spotted wilt virus (TSWV, transmitted by thrips). The ToMV-infected samples were mostly co-infected with either TYLCV or ToCV. Mixed infections of different combinations of TYLCV, ToCV and ToMV were detected with the mixed infection of two whitefly-transmitted viruses (TYLCV and ToCV) causing the most severe symptoms. According to the CP sequence of each virus, the 100% identities were shown to be Mexico/ABG73017.1 (TYLCV), Greece/CDG34553.1 (ToCV), China/AKN79752 (TSWV), and Australia/NP078449.1 (ToMV). Based on the sequence data, we presumed that these tomato infecting viruses were transmitted through insects and seeds introduced from neighboring countries.

• Research in Plant Disease
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• v.22 no.4
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• pp.297-302
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• 2016

Tomato yellow leaf curl virus (TYLCV) is a viral disease causing severe economic losses on tomato. Practical prevention of the TYLCV disease is to control tabacco whitefly (Bemisia tabaci) or to cultivate TYLCV-resistant tomato cultivars, because no agrochemical products are available to control TYLCV. In this study, TYLCV resistance of the commercial tomato cultivars were evaluated using the DNA markers tightly linked to TYLCV resistance genes Ty-1 and Ty-3 and infection with the TYLCV clones mediated by Agrobacterium. In marker genotyping, resistance alleles were detected from 4 oval type tomato cultivars (Titichal, TY tinny, TY saengsaeng II, TY sense Q). Four cheery type cultiavrs (TY endorphin, TY smartsama, Tiara TY, Olleh TY) and 6 round type cultivars (TY kingdom, TY ace, TY homerun, TY altorang, Dotaerang TY winner, Styx TY). The seedling bioassay indicated that tomato cultivars of the oval type and cherry type showed consistancy in marker genotype and phenotype while slight disease symptom was observed from some round type cultivras (TY ace, TY homerun, Styx TY) with resistance marker genotype. For fruit yields, TY tinny was greater than its control cultivar Titichal in oval types, TY smartsama was greater than its control Smile in cherry type, and TY ace and TY kingdom were greater than their control Dabok. These cutliavrs can be a good choice for high-yielding TYLCV-resistant tomato cultivars.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.507-517
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• 2019

To follow up on a 2017 survey of tomato virus diseases, samples with virus-like symptoms were collected from the same areas (Buyeo-gun, Chungchungnam-Do and Daejeon, Korea) in 2018. While in 2017 mixed infections of Tomato mosaic virus with either Tomato yellow leaf curl virus (TYLCV) or Tomato chlorosis virus were detected, only TYLCV was detected in symptomatic samples in 2018. TYLCV amplicons of c.777 bp representing the coat protein (CP) coding region were cloned from the TYLCV positive samples, and the sequence data showed a 97.17% to 98.84% nucleotide and 98.45% to 99.22% amino acid identity with the 2017 Buyeo-gun isolate (MG787542), which had the highest amino acid (aa) sequence identity of up to 99.2% with four 2018 Buyeo-gun sequences (MK521830, MK521833, MK521834, and MK521835). The lowest aa sequence identity of 98.45% was found in a 2018 Daejeon isolate (MK521836); the distance between Buyeo-gun and Daejeon is about 45 km. Phylogenetic analysis indicated that the currently reported CP sequences are most closely related to Korean sequences from Masan (HM130912), Goseong (JN680149), Busan (GQ141873), Boseong (GU325634), and the 2017 isolate TYLCV-N (MG787543) in the 'Japan' cluster of TYLCV isolates and distinct from the 'China' cluster isolates from Nonsan (GU325632), Jeonju (HM130913) and Jeju (GU325633, HM130914). Our survey data from 2017 and 2018 suggest that TYLCV has become established in Korea and may be spread by whitefly vectors from weed reservoirs within the farm environment.

• Research in Plant Disease
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• v.28 no.1
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• pp.39-45
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• 2022

Patterns of occurrence of tomato yellow leaf curl virus (TYLCV) and tomato chlorosis virus (ToCV) with whitefly on summer-cultivated tomato in Gwangju-si, Gyeonggi Province were surveyed using multiplex reverse transcription-polymerase chain reaction in 2020. In addition, distribution of the whiteflies species and their viral transmission rates were investigated throughout the tomato growing season. The infection rates of TYLCV and ToCV increased sharply during harvest, and the single infection rates were 30.9% and 5.0%, respectively, with a mixed infection rate of the two viruses being the highest at 52.2%. Single infection with TYLCV and double infections with TYLCV and ToCV accounted for the majority with 83.1%. Bemisia tabaci were dominant over Trialeurodes vaporariorum in greenhouse grown plants, and all of the investigated B. tabaci biotypes were identified as Mediterranean (MED, formerly known as Q biotype). The transmission rate of TYLCV, detected in every sampled B. tabaci MED population, was 21.4%, and the mixed transmission rate with ToCV was 35.5%. Viruliferous MED whiteflies with ToCV showed a higher rate than that of T. vaporariorum. In the transplant stage, viruliferous rate of both TYLCV and ToCV of B. tabaci was 42.7%; this rate was highest in the harvest stage. In examination of tomato yield, the increase in the mixed infection rate of TYLCV and ToCV led to complete yield loss. When the mixed infection rate increased by 10%, the yield decreased by 405.4 kg/10a.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.10a
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• pp.125-131
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• 2003

The U-draw bending operation is known as a representative test method for springback evaluation of sheet metals since the sheet in U-draw bending operation undergoes stretching, bending and unbending deformations occurred at read stamping process. In this study, a simplified approach was proposed for predicting springback and side-wall curls in U-draw bending operations, using moment-curvature relationships derived for sheets undergoing stretching, bending and unbending deformation.

• Korean Journal of Mathematics
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• v.18 no.4
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• pp.425-440
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• 2010

Using Green's theorem, elliptic boundary value problems can be converted to boundary integral equations. A numerical methods for boundary integral equations are boundary elementary method(BEM). BEM has advantages over finite element method(FEM) whenever the fundamental solutions are known. Helmholtz type equations arise naturally in many physical applications. In a boundary integral formulation for the exterior Neumann there occurs a hypersingular operator which exhibits a strong singularity like $\frac{1}{|x-y|^3}$ and hence is not an integrable function. In this paper we are going to remove this hypersingularity by reducing the regularity of test functions.