• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.6
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• pp.812-813
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• 1995

Lactococcal cells are nutritionally fastidious and thus, generally cultured either in milk or M17 medium (Terzaghi and Sandine, 1975). In this study, Lactococcus cremoris wild-type (KH) and its less­proteolytic mutant (KHA1) cells were grown on the M17 medium or with modified M17 medium by replicated parallel experiments. The modified M17 medium had the same composition as M17 medium, except that lactose was replaced by glucose. Analyses of culture-broth samples, in which the M17 and the modified M17 media were used, were conducted by high-performance liquid chromatography (HPLC). But, working with these media created noisy problems in analyses of samples. Therefore, a new semi-synthetic medium was developed on the basis of nutritional requirements (Morishita et al., 1981). The composition of the semi-synthetic medium determined on the basis of the nutritional requirements and the composition of milk, is presented in Table 1. The composition of M17 medium is also presented and compared in the table. L. cremoris KH and KHA1 cells were grown again on the new synthetic medium containing glucose or lactose. The broth samples were then drawn and analyzed by HPLC. Clearer separations of fermented products were achieved from the new medium than those with the M17 and the modified M17 media. In comparison with the M17 or the modified M17 media, growth on the new medium was good (Kim et al, 1993). Additional fermentations were also carried out at a controlled pH of 7.0, where enhanced growth of lactococcal cells was obtained. In the fermentations, samples were also analyzed for the concentrations of sugar and lactic acid. The results showed that the new synthetic medium was as good as or better than the M 17 and the modified M 17 media. This is because casein hydrolysate in the synthetic medium provided a ready supply of amino acids and peptides for L. cremoris KH and KHA1 cells. Lactic acid bacteria (LAB) including Lactococcal cells have been known to be an effective means of preserving foods, at the same time as giving particular tastes in fields of dairy products. LAB also have always occupied an important place in the technology of sea products, and marine LAB have known to be present in traditional fermented products (Ohhira et al, 1988). To apply the new synthetic medium to marine LAB, two different LAB were isolated from pickled anchovy and pollacks caviar and were grown on the new media in which various concentrations of NaCl $(3, 5, 7 and 10\%)$ added. They were also grown on the medium solution in natural seawater $(35\%o\;salinity)$ and on the solution of natural seawater itself, too. As seen in Fig. 1, Marine LAB were grown best on the synthetic medium solution in natural seawater and the higher concentrations of NaCl were added to the medium, the longer lag-phase of growth profile appeared. Marine LAB in natural seawater were not grown well. From these results, the synthetic medium seems good to cultivate cells which are essential to get salted fish aged. In this study, it showed that the new synthetic medium provided adequate nutrition for L. cremoris KH and KHA1 cells, which have been used as cheese starters (Stadhouders et al, 1988). Using this new medium, the acid production capability of starter cultures could be also measured quantitatively. Thus, this new medium was inferior to the M17 or the modified M17 medium in culturing the cheese starters and in measuring fermentation characteristics of the starter cells. Moreover, this new medium found to be good for selected and well-identified marine LAB which are used in rapid fermentations of low-salted fish.

• Journal of Aquaculture
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• v.11 no.2
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• pp.193-201
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• 1998

This study was carried out to investigate the enviromental quality and the growth of transplanted pen shell, Atrinna pectinata japonica. Followings are the results of growth of transplanted pen shell with respect to the shell size groups from the natural habitat (Usando) in May 1995, and cultivated upto November in the transplantated area (Soomoonri). The water depth of transplantated area andnatural habitat were 3m, 20~25m, respectively. The seawater temperature of the two culturing farms were ranged of 10.9~$27.8^{\circ}C.$, 8.5~$30.0^{\circ}C.$, respectively at the lowest in November adn the highest in July. The seawater salinity of the two areas were ranged of 29.54~35.26$^0\prime\infty$, 28.75~36.31$^0\prime\infty$, respectively at the lowest in July and the highest in November. The phosphoric acid ($PO_4$-P) of the two areas were 0.09~$1.14 ^{\mu}$g-at/l, 0.23~$1.33 ^{\mu}$g-at/l, respectively at the lowest in June and the highest in September. The bottom type of the two areas was a silty mud, 85.23% (82.17~87.26%) in natural habitat and 92.12% (90.76~92.94$^0\prime\infty$) in transplanted area. In this study area, phytoplankton were composed of 19 species. Of these 19 species, Skeletonema costatum was dominant species in seawater between natural habitat and transplantatied area, and 157 cells/ml, 165 cells/ml at August respectively. Stock of phytoplankton in transplantated area were more than those of natural habitat except June and November. The growth of shell length, shell height, total weight, soft part weight and posterior adductor muscle weight of pen shell on different size groups (SL 10, 10~15, 15~20, 20cm) were excellent in shell length of 10cm group, and 99.32%, 107.66%, 871.09%, 951.26% and 1,223.76%, respectively. The survival rate of pen shell was 98.10% in the shell length of 10cm groups, 90.35~94.76% in the others groups. The growth of shell length, total weight, soft part weight and posterior adductor muscle weight of pen shell in transplantated area were more 1.3, 2.6, 2.7 and 4.5 times than those of natural habitat.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.25 no.2
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• pp.26-41
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• 2020

In order to culture a life for the physiological and ecological research of the meiofauna, this study aimed to identify the most ideal condition in which the meiofauna can be cultured within a laboratory by setting various environmental conditions. The sediment deposits and seawater were collected from the intertidal zone in Mallipo of the west coast. A aquarium in which the internal environment can be controlled by constantly maintaining the temperature and humidity was fabricated and the culture experiments of the collected meiofauna were conducted together with the sea water and sediment deposits collected. The experiment 1 was conducted after establishing the similar environment as the collecting location. Under the same condition as the experiment 1, the experiment 2 verified a difference between when live foods were supplied and were not. In the experiment 3, the changes in the meiofauna colony were checked according to with or without light and live foods. In the results of culturing experiments, the habitat density and the number of appeared classification groups of the meiofauna colony were relatively higher both in the water tank with supplying the live foods and under the condition of having light in 12-hour cycle than those in the aquarium without live foods and under no light condition. In addition, the habitat density of meiofauna cultured within a laboratory exhibited relatively higher value than that under the natural state.

• Journal of fish pathology
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• v.19 no.1
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• pp.65-72
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• 2006

Nervous necrosis virus (NNV) that causes severe mortality during seed production of red drum (Sciaenops ocellatus) is known to be vertically transmitted from infected spawners. This study was conducted to produce NNV free seeds by testing spawners for NNV infection and using virus free eggs for seed production. RT-PCR analysis of 40 spawners showed 18 positives and 22 negatives. NNV was not detected from eggs obtained from the negative spawners but was detected from those obtained from the positives. NNV was not detected from culturing seawater in tanks and Chlorella spp., Brachionus plicatilis., and Brine shrimp those were provided as live feed. The survival rates of fry from NNV positive and negative spawners were 80% and 85%, respectively by two weeks after hatching. The mortality increased from 25days after hatching and the final survival rate of seeds from NNV positive and negative spawners were 0% and 18.3%, respectively on 41 days after hatching. These results suggested that virus free red drum seeds can be obtained by using virus-free spawners.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.6
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• pp.570-581
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• 2013

To improve productivity of aquaculture animals with management of culturing grounds, survey of mariculture management area in Ongjin-gun about water quality and sedimentary environment had been conducted on June, August and November in 2011. Water temperature in surface and bottom waters ranged from 9.49 to $24.14^{\circ}C$. Salinity and dissolved oxygen concentrations were in the range of 23.19~31.49 and 5.48~9.36 mg/L, respectively, depending on the variation of water temperature. The average concentration of COD was 1.57 mg/L and the concentrations of DIN and DIP showed entirely low level. As the result of grain size analysis, sand(56.66 %) and silt(34.60 %) were predominated. The Mz of sediment showed a variation of 2.59 to $6.62{\O}$ and sorting appeared to be poorly sorted. The concentrations of COD and IL in surface sediment ranged from 1.00 to $11.03mg/g{\cdot}dry$ and 0.72 to 5.29 %, respectively, which showed relatively good positive correlations. On the environmental assessment of trace metals in surface sediment, geoaccumulation index ($I_{geo}$) class indicated that sediments were not contaminated by most of metallic elements except Cr and As. Our result implies that this study area showed good water quality and sediments were not polluted by organic matters and metallic elements.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.14 no.4
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• pp.213-228
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• 2009

This study was conducted to investigate spatio-temporal changes in macrobenthic community structure and benthic environmental conditions in Geoje-Hansa Bay, which is the greatest oyster producing site in Korea. Field survey for benthic environment and macrobenthos was seasonally carried out at 15 stations covering oyster farming sites and non-farming sites from February to November, 2008. The grain size of surface sediments was dominated by very fine silt with the mean phi of about $9\;{\Phi}$ and TOC was 1.9% on average. Mean dissolved oxygen content was 8.1 mg/L and lowest in August corresponding to the 2nd degree in seawater quality criteria. Total species number was 351 and mean density was $3,675\;ind./m^2$, both of which were dominated by polychaete worms. Spatio-temporal variation in above two biological variables was great with higher values seasonally in spring and spatially in channels rather than inner bay. Dominant species were Lumbrineris longifolia (21.3%), Aphelochaeta monilaris (17.8%) and Ericthonius pugnax(6.1%), all of which are typical species of organically enriched area. From the multivariate analyses, the whole macrobenthic community was distinguished into two groups of channel and inner bay group. Spatio-temporal changes of macrobenthic community in Geoje-Hansan Bay were related to those of TOC and acid volatile sulfide (AVS). Our results showed that Geoje-Hansan Bay should be intermediately affected by organic pollution, and that such organic enrichment was more remarkable at farming stations in the inner bay.