Browse > Article

Nervous necrosis virus (NNV) -free seed production of red drum, Sciaenops ocellatus  

Kim, Jin-Do (국립수산과학원)
Jung, Sung-Ju (전남대학교)
Oh, Bong-Se (국립수산과학원)
Park, Sung-Woo (군산대학교)
Oh, Myung-Joo (전남대학교)
Kim, Young-Jin (여수대학교)
Kitamura, Shin-Ichi (여수대학교)
Byun, Soon-Gyu (국립수산과학원)
Publication Information
Journal of fish pathology / v.19, no.1, 2006 , pp. 65-72 More about this Journal
Abstract
Nervous necrosis virus (NNV) that causes severe mortality during seed production of red drum (Sciaenops ocellatus) is known to be vertically transmitted from infected spawners. This study was conducted to produce NNV free seeds by testing spawners for NNV infection and using virus free eggs for seed production. RT-PCR analysis of 40 spawners showed 18 positives and 22 negatives. NNV was not detected from eggs obtained from the negative spawners but was detected from those obtained from the positives. NNV was not detected from culturing seawater in tanks and Chlorella spp., Brachionus plicatilis., and Brine shrimp those were provided as live feed. The survival rates of fry from NNV positive and negative spawners were 80% and 85%, respectively by two weeks after hatching. The mortality increased from 25days after hatching and the final survival rate of seeds from NNV positive and negative spawners were 0% and 18.3%, respectively on 41 days after hatching. These results suggested that virus free red drum seeds can be obtained by using virus-free spawners.
Keywords
Nervous necrosis virus; seed production; red drum; survival rate; virus-free spawner;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bowden, R. A., Oesstmann, D. J. Lewis, D. H. and Frey, M. S.: Lymphocystis in red drum. J. Aquat. Anim. health, 7(3): 231-235,1995   DOI   ScienceOn
2 Kim, S. R., Jung, S. J., Kim, Y. J., Kim, J. D., Jung, T. S., Choi, T. J., Yoshimzu, M. and Oh, M. J.: Phylogenie Comparison of Viral Nervous Necrosis (VNN) Viruses Occurring Seed Production Period. J. Korean Fish. Soc., 35(3): 237-241,2002
3 Munday, B. L. and Nakai T.: Some diseases of economic significance in Austrilian finfish aquaculture. International symposium on aquatic animal health: Program and abstracts, Univ. of California, School of Vertenary Medicine, Davis, CA (USA), p. W-22.6,1994
4 Pavoletti, E., M. Prearo, M. Ghittino and C. Ghittino: Cases of viral nervous necrosis (VNN) in shy drum, Umbrina cirrosa with description of clinical symptomatology and anatomohistopathological features. Boll. Soc. Ital. Patol. Ittica., 10(23): 24-33, 1998
5 Sohn, S. G., Park, M. A., Oh, M. J. and Chun, S. K.: A fish Nodavirus isolated from cultured sevenbanded grouper, Epinephelus septemfasciatus. J. Fish Pathol., 11(2): 97-104, 1998
6 손상규, 전세규: 능성어, Epinephelus septemfasciatus의 바이러스성 신경괴사증 바이러스의 병원성 연구, 한국어병학회지, 12(2): 107-113, 1999
7 蟲明敬一, 中井敏博, 室賀淸邦, 關生, 古澤嚴: シマアジのウイルス性神經壤死症: 仔魚の發病に對する抗體および産卵飼育方法の影響, 水産增殖, 41(3): 327-332, 1993
8 T. Nishizawa, Mori K., Nakai T., Furusawa I. and Muroga K.: Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Dis. Aquat. Org., 18(2): 102-107,1994
9 오명주, 김석렬, 정성주, 김형락, 김흥윤, 여인규: 한외여과막을 이용한 해수내 어류 병원 바이러스 농축법. 한국어병학회지. 13(1): 61-66, 2000
10 Caray, O. Cunningham: Molecular diagnosis of fish and shellfish disease: Present status and potential use in disease control. Aquaculture, 206: 19-25,2002   DOI
11 室賀淸邦: 疾病防除の面かろみに 放流用種苗生産のあり方, 培技硏,28(1): 39-45, 2000
12 Kim, J. D., Kim, S. R., Jung, S. J., Kim, Y. J., Jung, T. S., Choi, T. J., Park, S. W. and Oh, M. J.: Occurrence of viral nervous necrosis (VNN) in red drum (Sciaenops ocellatus) larvae. J. Fish Pathol., 14(2): 91-95, 2001
13 T. Nishizawa, M. Furuhashi, T. Nagai, T. Nakai and K. Muroga: Genomic Classification of Fish Nodaviruses by Molecular Phylogenetic Analysis of the Coat Protein Gene. Applied and Environmental Microbiology, 1633-1636,1997
14 蟲明敬一: シマアジ 親魚の産卵に伴つて增殖とその性神經壤死症 (VNN) 原困 (SJNNV) 抑制交果, 水産增殖, 48(1): 109-115.,2000
15 Bovo, G., T. Nishizawa, C. Maltese, F. Borghesan, F. Mutinelli, F. Montesi and S. De Mas.: Viral encephalopathy and retinopathy of farmed marine fish species in Italy. Virus Res., 63: 143-146,1999   DOI   ScienceOn
16 Munday, B. L. and T. Nakai: Special topic review: nodaviruses as pathogens in larval and juvenile marine finfish. World J. Microbiol. Biotechnol., 13: 375-381, 1997   DOI   ScienceOn
17 室賀淸邦. 古澤 澈, 古澤 嚴: シマアジのウイルス性神經壤死症. 水産增殖, 46(4): 473-480, 1998
18 Zafran, Isti Koesharyani, Fris Johnny, Kei Yuasa, Takihiko Harada and Kishio Hatai: Viral nervous Necrosis in Humpback Grouper Cromileptes altivelis Larvae and Juveniles in Indonesia. Fish Pathology, 35(2): 95-96, 2000   DOI
19 Arimoto, M., Mori, K., Nakai, T., Muroga, K. and Furuzawa, I.: Pathogenicity of the causative agent of viral nervous necrosis disease in striped jack, Pseudocaranx dentex (Bloch and Schneider). Journal of fish diseases, 16(5): 461-469,1993   DOI   ScienceOn
20 Watanabe, K., T. Nishizawa and M. Yoshimizu: Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus. Dis. Aquat. Org., 41(3): 219-223,2000   DOI
21 Watanabe, K., Suzuki, S., Nishizawa, T., Suzuki, K., Yoshimizu, M. and Ezura, Y.: Control strategy for viral nervous necrosis of barfin flounder. Fish Pathol., 33(4): 445-446,1998   DOI
22 Oh, M. J., Jung, S. J., Kim, S. R., Rajendran, K. V., Kim, Y. J., Choi, T. J., Kim, H. R. and Kim, J. D.: A fish nodavirus associated with mass mortality in hatchery-reared red drum, Sciaenops ocellatus. Aquaculture, 211(1): 1-7, 2002   DOI   ScienceOn
23 Mori, K-I., Nakai, T., Muroga, K., Arimoto, M., Mushiake, K. and Furusawa, I.: Properties of a new virus belonging to Nodaviridae found in larval striped jack, Pseudocaranx dentex with nervous necrosis. Virology, 187(1): 368-371, 1992   DOI   ScienceOn