• Fisheries and Aquatic Sciences
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• 제5권1호
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• pp.1-4
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• 2002

The modulatory effects of oxytetracycline treatments at high concentrations on the infection potential of scuticociliates in cultured juvenile olive flounder (Paralichthys olivaceus) and density of the ciliates in culturing water were investigated. The groups bathed with 400 and 500 ppm of oxytetracycline showed significantly lower intensities of scuticociliates on the fish and considerably lower number of the ciliates in culturing water when compared with the control group. However, the intensity of scuticociliates on the fish in the group bathed with 300 ppm of oxytetracycline was not significantly different with that of the control group in spite of considerably lower number of scuticociliates in culturing water than in that of the control group. Although the intensities of scuticociliates on the fish intubated orally with 400 and 500 mg/kg of oxytetracycline were lower than that of the control group, there were no statistical significances. In contrast, the fish fed 300 mg/kg of oxytetracycline showed significantly lower intensity of scuticociliates when compared with other groups. The results of this study suggest that oxytetracycline treatments can modulate occurrence of scuticociliatosis in fish farms probably through change of bacterial density, damaging to scuticociliatosis and immuno-suppression of fish.

• 한국동물위생학회지
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• 제20권1호
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• pp.11-18
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• 1997

Characteristics, pathogenicity and control of the causative organisms isolated front diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF-1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KBF-1) with $1.0{\times}10^7$ cfu/fish were died within 60 hrs, but 0~9 flounders infected group with 41.0{\times}10^6$ cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with $1.0{\times}10^7$ cfu/fish were died within 36 hrs, while 0~7 flounders infected with $1.0{\times}10^6$ cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-1 strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-1 strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.

• 대한수의학회지
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• 제47권3호
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• pp.303-308
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• 2007

The high mortality (65.5%) of young olive flounder (Paralichthys olivaceus) showing skin ulcers occurred in fish obtained from a commercial cultured farm at Donghae on the eastern coast of Korea at March 2006. The dorsal skin showed ulcer lesions and large amount of mucus. We isolated Ichthyobodo sp. from the gill, and Vibrio anguillarum from the liver and kidney of dead and diseased fish. All of the fish infected with the V anguillarum and Ichthyobodo sp. were treated with 100 ppm formalin for 1 h, and treated with oxolinic acid for 4 h at $22^{\circ}C$, respectively. The formalin treated group was observed the low mortality (2.9%) when compared with high mortality of non-treated (82.9%), and of antibiotic treated group (85.9%) against mixed infections by Vibrio anguillarum and Ichthyobodo sp. organisms. We confirmed complete elimination against mixed infections of Vibrio anguillarum and Ichthyobodo sp. with 100 ppm formalin treatment.

• 한국수산과학회지
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• 제47권4호
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• pp.406-412
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• 2014

To evaluate the influence of burrowing substrate on the rearing performance and ambicoloration of cultured flounder, Paralichthys olivaceus, we compared the daily food intake (DFI), feed efficiency (FE), survival, growth, proportion of pigmented skin on the blind side, and proportion of ambicolored fish. We reared juvenile flounders [total length (TL) $4.46{\pm}0.06cm$, body weight (BW) $0.77{\pm}0.03g$] in dark-green fiberglass-reinforced plastic (FRP) aquariums without (control) or with gravel substrate at a density of 200 fishes/ton for 120 days. While there was no difference in survival rate or growth, the DFI was lower and FE higher in the group raised with substrate than in the control. The proportions of pigmented area on the blind side and ambicolored fish were significantly higher in the control tank. Therefore, the supplement of substrate on the aquarium bottom positively affects the feeding efficiency, and inhibits abnormal pigmentation on the blind side in flounder farming at high density.

• 한국어병학회지
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• 제18권2호
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• pp.119-124
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• 2005

Two species of myxosporean parasites - Parvicapsula anisocaudata and an unidentified myxosporean were found in the lumina of renal tubules and the tubular epithelium, respectively, from cultured olive flounder, Paralichthys olivaceus in Korea. The latter was also seen in interstitial tissue of spleen and interrenal gland of the head kidney. Group of pseudoplasmodia of P. anisocaudata were firmly attached on the epithelium of renal tubules through pseudopodia. In the renal tubule epithelium, a group of unidentified myxosporean trophozoites, which were 2-3 times larger than intraluminal trophozoites of P. anisocaudata, was observed. The parasites being burst out into the lumen was occasionally encountered with partial break of the epithelium. Although infection of P. anisocaudata and unidentified myxosporean parasites did not induce any cellular reaction of the host, occlusion of renal tubules and rupture of renal epithelium would impact negatively on the renal functions of severely infected fish.

• 한국수산과학회지
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• 제53권5호
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• pp.740-751
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• 2020

In the aquaculture industry, few studies are analyzing big data for intrinsic meaning. Fishcare Laboratory (www.fishcare.kr) diagnostic data from 2016-2018 was analyzed for scuticociliatosis (caused by Miamiensis avidus) outbreak patterns in cultured olive flounder Paralichthys olivaceus in Jeju, Korea. The scuticociliatosis monthly occurrence ratio is reported in the summary table after preparing and filtering the basic dataset model. Nonparametric test results suggest differences in the water temperature, body length, and weight between groups with and without scuticociliatosis. Data distribution visualization revealed that shorter body length and lighter weight increased the occurrence of scuticociliatosis. The association rule mining technique was applied to determine the primary clinical signs of mixed scuticociliatosis and bacterial infections. Venn diagrams were used to report clinical signs and suggest commonalities. These results may help diagnose and treat fish and provide a decision-making reference.

• 한국어병학회지
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• 제13권2호
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• pp.143-146
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• 2000

본 연구에서는 bronopol과 bithionol을 이용하여 양식 넙치에 기생하는 Ichthyobodo sp.의 치료효과를 조사하였으며, 그 효과를 formalin과 비교하였다. formalin 200 ppm 혹은 bithionol 100 ppm에서 1 시간동안 약욕한 결과 모든 실험어에서 100% 치료효과를 나타내었으며, 이보다 더 낮은 농도(formalin 50 ppm, 100 ppm 및 bithionol 25 ppm, 50 ppm)에서의 약욕에 의해서도 Ichthyobodo sp.의 감염강도를 유의적으로 감소시켰다. 그러나 bronopol을 이용한 약욕 및 bithionol의 경구투여는 대조구에 비해 유의적인 치료효과를 나타내지 않았다.

• Parasites, Hosts and Diseases
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• 제57권2호
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• pp.127-134
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• 2019

Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient morphological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary bladder of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were observed under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring $13.9{\pm}0.6{\mu}m$ in length and $13.8{\pm}0.8{\mu}m$ in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of $4.3{\pm}0.4{\mu}m$. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsularis. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.

• 한국식품조리과학회지
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• 제13권4호
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• pp.448-452
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• 1997

양식넙치의 식감을 검토하고자 천연어와 비교하여 일반성분, 관능검사, 물성측정, 정미성분을 측정한 결과는 다음과 같다. 1. 양식넙치는 수분, 지질, 단백질 함량에 천연어와 차이가 없었다. 2. 기호검사에서 양식어는 생육, 가열육 모두 Texture, 맛, 종합적 선호도에서 천연어보다 유의적으로 선호되지 않았고, 식별검사에는 생육은 경도, 탄력성, 맛의 강도가 낮았고, 가열육은 천연어보다 매우 단단하고 건조하며 맛의 강도가 약한 것으로 평가되었다. 투명감, 광택, 비린내는 두어종 사이에 큰 차이가 없었다. 3. 물성측정의 결과도 생육은 천연어보다 경도, 탄력성이 낮게, 가열육은 경도가 매우 높게 나타나 관능검사의 결과와 유사한 경향을 보였다. 4. 어육의 정미성분으로서 맛과 관련있는 유리아미노산은 Alanine이 양식어 7.0mg%, 천연어 7.4mg%로 가장 많았고, Glutamic acid, Proline, Methionine, Glycine의 순으로 함유되어 있었으며, 이 아미노산들의 함유량 합계는 차이가 없었다. 핵산관련물질은 ATP가 양식어 3.4 $\mu$mole/g, 천연어 6.2 $\mu$mole/g으로 가장 많이 함유되어 있었고, IMP, ADP, AMP순으로 함유되어 있었으며, 이들의 합계량은 양식어가 6.7 $\mu$mlole/g, 천연어가 9.4 $\mu$mole/g으로 천연어에 비해 낮았다.

• 대한수의학회지
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• 제44권4호
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• pp.643-648
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• 2004

Efficacy of ciprofloxacin was evaluated in laboratory and field studies for control of streptococcal infection in flounder and eel. In disc diffussion test, all streptococcal strains showed high sensitivity to the ciprofloxacin. Minimum inhibitory concentrations of ciprofloxacin against all streptococcal strains used in this study were less than $0.195{\mu}g/ml/$. In laboratory studies where fishes were challanged with Streptococcus iniae, significant reductions in mortality were observed among fish receiving ciprofloxacin (in fed) at 100 mg/kg/body weight or more daily for 3 d when compared with mortality of non-medicated controls. Similar results were achieved when 100 mg/kg/body weight of ciprofloxacin was used in field trials.