• Title/Summary/Keyword: Culture type

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Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type I

  • Kim, Su-Nam;Min, Kwan-Ki;Kim, Seung-Hwan;Choi, In-Hwa;Lee, Suhk-Hyung;Pyo, Suhk-Noung;Rhee, Dong-Kwon
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.179-183
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    • 1996
  • Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/1 concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.

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Analysis of the MAT1-1 and MAT1-2 Gene Ratio in Black Koji Molds Isolated from Meju

  • Mageswari, Anbazhagan;Kim, Jeong-seon;Cheon, Kyu-Ho;Kwon, Soon-Wo;Yamada, Osamu;Hong, Seung-Beom
    • Mycobiology
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    • v.44 no.4
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    • pp.269-276
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    • 2016
  • Aspergillus luchuensis is known as an industrially important fungal species used for making fermented foods such as awamori and shochu in Japan, makgeolli and Meju in Korea, and Pu-erh tea in China. Nonetheless, this species has not yet been widely studied regarding mating-type genes. In this study, we examined the MAT1-1 and MAT1-2 gene ratio in black koji molds (A. luchuensis, Aspergillus niger, and Aspergillus tubingensis) and in Aspergillus welwitschiae isolated from Meju, a fermented soybean starting material for traditional soy sauce and soybean paste in Korea. The number of strains with the MAT1-1 locus was 2 of 23 (A. luchuensis), 6 of 13 (A. tubingensis), 21 of 28 (A. niger), and 5 of 10 (A. welwitschiae). Fungal species A. tubingensis and A. welwitschiae showed a 1 : 1 ratio of MAT1-1 and MAT1-2 mating-type loci. In contrast, A. luchuensis revealed predominance of MAT1-2 (91.3%) and A. niger of MAT1-1 (75%). We isolated and identified 2 A. luchuensis MAT1-1 strains from Meju, although all strains for making shochu in Japan are of the MAT1-2 type. These strains may be a good resource for breeding of A. luchuensis to be used in the Asian fermented-food industry.

The Effects of VM Components on Store Image and Purchasing Intention of Fashion Stores - Focused on Facade Color and Show Window Type - (의류매장의 VM 구성요소가 점포이미지와 구매의도에 미치는 영향 - 파사드 색상과 쇼 윈도우 유형을 중심으로 -)

  • Lee, Mi-Sook
    • The Research Journal of the Costume Culture
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    • v.20 no.3
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    • pp.416-429
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    • 2012
  • The purpose of this study was to examine the effects of VM components(facade color and show window type) on store image and purchasing intention of fashion stores. The subjects were 428 male and female university students in Daejeon and Chungnam province. The measuring instruments were 6 stimuli manipulated by $3{\times}2$ factorial design of facade color and show window type, and a self-administrated questionnaire consisted of store image, purchasing intention, and subjects' demographic characteristics. The results were as follows. First, facade color mainly affected store image and purchasing intention. White color was preferred as more positive images(pleasurable, comfortable, neat, and modern), and showed higher purchasing intention than brown color. Second, show window type affected some store images. Semi-open type was perceived as more pleasurable image than enclosed type, whereas enclosed type was perceived as more luxurious, neat, and attractive image than semi-open type. However, show window type didn't affect purchasing intention. Third, subject' sex affected store image and purchasing intention. Male subjects perceived more positively on store image and had higher purchasing intention than females. This study suggested that facade color and show window type are important VM components affecting store image and purchasing intention.

OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE TYPE I

  • Kim, S.N.;K.K. Min;Kim, S.H.;Park, I.H.;Lee, S.H.;S.N. Pyo;D.K. Rhee
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.186-186
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    • 1996
  • Streptococcus pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide(PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsular PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was used to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not enhance growth or PS production. Substitution of nitrogen sources with other resources or supplemention of various concentrations of metal ion (with the exception of calcium ion) had adverse effects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/I concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synergistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cumulative increase in capsular PS production.

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Current Status of Lighting System and Illumination Distribution in the Greenhouse for Light Culture of Strawberries (딸기 전조재배 온실의 인공광 설치실태 및 조도분포 조사분석)

  • 김태한;장익주;이경진
    • Journal of Biosystems Engineering
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    • v.24 no.1
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    • pp.19-24
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    • 1999
  • Motivated by the need for developing the more efficient lighting system for light culture of strawberries in the greenhouse, this paper aims at acquiring and suggesting more concrete and scientific foundation of illuminating position, numbers of light source by investigating the types of lighting system and illumination distribution in the greenhouse for light culture of strawberries. The results of investigation and experiment are summarized as following below: 1. The types of lighting system used in the greenhouse producing strawberries were classified as 1 line and 2 lines lighting system. 2. As for the arranging types of artificial light, 2 lines lighting system, were classified as Z-type, N-type and W-type. (Refer Fig. 3) 3. The results of illumination distribution for Z-type, N-type and W-type of 2 lines illuminating system in the greenhouse with a small size tunnel measured at the height of 1.5m from the ground with 220V, 100W lamp in 6m light gap showed that maximum illuminance are 961x, 1211x, 1251x, minimum illuminance are 4.41x, 4.71x, average illuminance are 33.71x, 43.11x, 44.51x and standard deviations are 28.31x, 35.41x, 38.31x at each types. 4. Proportion of the area below optimal illuminance to floor area at the two lines illuminating system of Z-, N-, and W-type in greenhouse were appeared as 39.4%, 26.0% and 26.3%, respectively. Also proportion of the area over optimal illuminance to floor area at the two lines illuminating system of Z-, N-, W-type in greenhouse were appeared as 16.8%, 14% and 14.7%, respectively. Thus N-type was superior to the others from the view points of optimal illumination distribution and energy saving.

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Analysis of the impact relationship between business culture's type and creating performance of convergence corporate (융합기업의 기업문화형태와 성과창출과의 영향 관계 분석)

  • Choi, Seung-Il;Kim, Dong-Il
    • Journal of Digital Convergence
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    • v.13 no.8
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    • pp.169-176
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    • 2015
  • This study try to analyze the relationship between the corporate culture type and performance about convergence corporate. In addition, we developed a model that fix this research to analyze the business culture and firms type in order to perform this study overall. In the daily operations of an organization's culture can be a source of corporate competitiveness, it may be classified as an important factor in the performance of the organization to promote. In other words, the organizational culture as well as be able to describe an individual's attitudes and behavior, microscopic, macroscopic analysis of organizational behavior can serve to connect conceptually each others and through this process can be seen as contributing to the achievement creation. Therefore, analyzed the influence of the relationship between corporate culture and performance form the basis of this study and research model was validated. Corporate culture in the form of the convergency enterprise found out the strong relationship with job satisfaction and organizational commitment in the research result. Finally, this research results are expected to be a useful guide as a basis for the creation and management of companies with a corporate culture in the near future convergence corporation.

A Comparative Study on the Characteristics by Type of Korean, Chinese and Japanese Traditional Furniture (한.중.일 전통가구의 유형에 따른 특성 비교연구)

  • Kim Jin-Ok
    • Archives of design research
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    • v.19 no.4 s.66
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    • pp.111-120
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    • 2006
  • These days, contemporary people's interest in culture is higher than ever, and in response to such a current trend, each country is making efforts to search for its identity in various areas, and conducting research to compare its culture with that of other countries. The culture of a region does not develop independently or spontaneously, but it changes and evolves in connection with neighboring countries or cultures. Thus, in order to understand our own cultural characteristics properly, we need not only to make an in-depth study in our own culture but also to compare it with other cultures and understand their similarities and differences dearly. From old times, Korea, China and Japan have had great influences on one another through active socio cultural exchange and at the same time they have their own unique cultural characteristics. Unfortunately, however, the distinguished identity of Korean culture has not been recognized properly. The present study purposed to classify Korean, Chinese and Japanese traditional furniture from the $14th{\sim}19th$ century by type, and compare their characteristics by type as well as their general formative characteristics. This research is expected to improve our objective understanding of our own culture. Furthermore, it will provide a ground for reinterpreting our culture to distinguish Korean culture from Oriental culture.

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A Comparisonal Study between Korean Po and Turkish Sultans′ Dress (우리나라 포(袍)와 터키 Sultans′의 의례용 외의 형태 비교 연구 - 1400∼1800년대를 중심으로 -)

  • 이상은;김영란
    • Journal of the Korea Fashion and Costume Design Association
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    • v.6 no.1
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    • pp.55-63
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    • 2004
  • The dress culture of Korea and Turkey should be necessary to study in view of a similar culture of Korea and Turkey due to similarity of oriental culture. Thus, as two nation's dress is investigated, cultural characteristics and dress form of two nations are analyzed with cultural background in detail. Also, comprehension is determinated for the dress color and texture as well as dress culture. In this paper, It is investigated theoretically for the function, type, and class of dress. Also, The form of Korean Po are compared to background of Turkish dress and Sulltans' dress form. To unify a cultural propagation and dress form, technical, inductive contents analytical method as well as literature review are used in positivism. Subject is limited to the Korean Po and Sultans' dress of Turkey. As a results, as the nation's dress reflect to culture and society of the nation, Korean dress have some similarity and differences comparing Turkish dress as followings. In dress form of two nation, rectangular Gil's shape was very similar. Mu's shape was similar too. However, Sultans' dress had outside shape of caftan type and Po had full or partial wrinkles by transforming Mu. Also, Sultans' dress did not have Sup. Korean Po have side slits or back side slits and Jun-Sam but Sultans' dress had only some side slits.

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An Approach on the Global Commerce Activation Regarding Culture Collision by the Use of M&A (M&A 협상성과에 기업간 문화차이가 미치는 영향에 관한 연구)

  • Park, Chong-Don
    • International Commerce and Information Review
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    • v.8 no.2
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    • pp.229-247
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    • 2006
  • The company culture is becoming usual term in business management field. That organization culture of these phenomenon becomes resource of company competitive power indeed, it is basing to logic for important leading person for elevation with systemicity. also, great many scholars do research to subject that is change management of company administration and there are not much example that companies succeed in change actually despite had announced treatise etc. This research tries to synthesize several research subjects by single model regarding confrontation(culture collision) phenomenon between culture that can happen in company through literature research and example analysis basically. Specially, try to classify and divide this by specific developing and arrange in each type with using much this culture analysis in negotiation analytics. Through this problem and suggestion point by this confrontation way in this culture negotiation side and culture risk management side of administration make try to.

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A Study on the Subjectivity of Consumers to Accept the Culture of Eating out through the YouTube Platform (유튜브 플랫폼을 통한 외식 문화 수용에 대한 소비자 주관성 연구)

  • Kim, Seong-Yong;Kim, Dong-Soo
    • The Journal of the Korea Contents Association
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    • v.20 no.9
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    • pp.414-423
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    • 2020
  • The purpose of This study is to investigate the effects of the acceptance of food culture through YouTube, one of the platforms that is currently being used, on consumers' perceptions, and to establish marketing effects that can be obtained from the acceptance of food culture through YouTube platform, Q-methodology was selected and exploratory research was conducted to analyze consumers' subjective perspectives. For this purpose, analysis was conducted by classifying statement cards by respondents, and Q-sorts were selected and composed by the Q-group, and after the statement was prepared through this, P-samples were selected and the Q-sorts obtained through classification work were analyzed through the Q factor analysis using the PC QUANL program. The analysis results were classified into four types: Type 1 [(N=6): Preferred Food and Responsibility Satisfaction Type], Type 2 [(N=3): Non-emotional Preferred Food Type, Type 3 [(N=14): Information Acquisition Preferred Food Type], Type 4 [(N=2): Undetermined Type], etc. Each subjective opinion from these analyses will be helpful for various studies in the future, and it will be used as a reference for YouTube-related marketing and consumer restaurant culture acceptance in the future.