• Title/Summary/Keyword: Culture type

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Ammonium Ion Effects and Its In Situ Removal by Using Immobilized Adsorbent in Hybridoma Cell Culture (하이브리도마 세포배양에서 암모늄 이온의 영향 및 고정화 흡착제에 의한 암모늄 이온의 동시제거)

  • 정연호;이해익
    • KSBB Journal
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    • v.11 no.3
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    • pp.329-339
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    • 1996
  • The effects of ammonium ion on cell growth kinetics, monoclonal antibody productivity, and cell metabolism of hybridoma cells were investigated. The mouse-mouse hybridoma cell line VlIIH-8 producing mouse IgG2a was used as a model system. Ammonium ion showed an inhibitory effect on cell growth and monoclonal antibody production. New immobilized adsorbents were developed for the reduction of the inhibitory effect of ammonium ion. The ammonium ion selective zeolite, Phillipsite-Gismondine was entrapped in calcium alginate bead or in dialysis membrane and applied to the hybridoma cell culture system for the in situ removal of ammonium ion from culture media. The effects of ammonium the both serum supplemented and serum free media on the cell growth were studied by applying immobilized adsorbents of calcium alginate bead type. The results demonstrated a substantial enhancement in cell growth. Applying immobilized adsorbents of dialysis membrane type to serum supplemented media also resulted in the stimulation of cell growth, cell viability and monoclonal antibody production.

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A Study on the Field Survey of the Korean Communities' Dwelling Houses of Rural Districts in East-Northern China - Longshancun, Yanbian - (동북지역 농촌의 조선민족 살림집의 실태조사 연구 -연변 룡정시 룡산촌을 중심으로-)

  • Rhee, Shin Ho;Kim, June Bong
    • Journal of the Korean Institute of Rural Architecture
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    • v.4 no.1
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    • pp.29-36
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    • 2002
  • The characteristics of the rural dwelling houses of the Korean communities were investigated and analyzed to understand their rural dwelling culture in east-northern China. This research was done by the field survey of rural village, Longshancun, Yanbian. The structures of their houses are changed into the brick houses from the earthen houses with wood frame. The floor plans are changed into Dual-bay-type of Divided-space from Single-bay-type of Hollistic-space, the largest ratio at present, and the numbers of space are increased. In the view of total outline, their dwelling houses are based to those of east-northern Korea and are sustained to the vernacular dwelling styles(ex : floor heating, ondol, and sit-down), and are adapted to the geography and climate of Yanbian and accepted to the culture of other communities(ex : indoor-kitchen, indoor-removing-shoes).

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Selection of mutant Phaffia rhodozyma and Determination of Optimum Culture Conditions for Astaxanthin Production (Astaxanthin 생산을 위한 Phaffia rhodozyma의 변이균주 선발과 최적 배양조건 결정)

  • 유성선;유연우
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.96-103
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    • 2001
  • Phaffia rhodozyma is the most promising microbial source of astaxanthin production, though wild-type strains are needed to increase the astaxanthin content for commercial production. To increase astaxanthin content for commercial production, a mutant strain of P. rhodozyma was selected and culture conditions of the mutant selected were optimized. P. rhodozyma was treated with mutagenic agent such as NTG, acriflavine, and UV in serial order and carotenoids hyper-producing mutant strain was selected based on the capabilities of cell growth on the agar plate containing chemical inhibitors and carotenoids production. Among the mutants tested, a mutant WS-2 was finally selected. Mutant WS-2 produced 1.26mg carotenoids/g-dry cell weight and this value was about- 4-folds higher than that of wild-type. The optimum culture conditions were $24^{\circ}C$ of temperature, 1.5vvm of aeration and 300rpm of agitation. In the optimized condition, cell and carotenoids concentrations were 7.62g/l and 14.9mg/l, respectively.

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The Study on the Modeling Development of Dwelling House of Islands in the Future Based on the Insularity and the Factor of Traditional Culture (도서성(Insularity)과 전통성에 바탕을 둔 이상적인 도서지역 주거모델 개발에 관한 연구)

  • Kim Ji-Min
    • Journal of the Korean housing association
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    • v.16 no.5
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    • pp.1-12
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    • 2005
  • This paper is architectural paper which has been studied about residential culture of Southwestern Island from 18C to now. It's main object is suggest a new residential type. Contents of research is 1, to survey a history and a cultural environment of island. 2. to survey and rearrange residence(traditional, modem) which has been built from the past to the present, and establish a history of residence and a theory. 3. to search for architectural space, shape(form), and architectural community, etc. which can be continued. 4. to suggests ideal future residence type of islands. Effectiveness of Research is 1. This study suggests a new theory and a new method to the field of residential science, especially to the residence of island area. 2. In this study, traditional theory of residence be reflected in the modern architectural design.

Comparative Study on Graphic Examples of Traditional Lattices in Korea, China, Japan - Focused on grid type from geometrical viewpoint - (한국, 중국, 일본의 전통 창살 그래픽 예제 비교 연구 - 기하학적 관점에서 그리드 형태를 중심으로 -)

  • 박지연;이연숙
    • Korean Institute of Interior Design Journal
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    • no.23
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    • pp.139-147
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    • 2000
  • The cultural identity of design is very critical in the modern society which is called the society of complex culture and multi-culture. In addition, adopting a traditional lattice-like pattern as a composition element of interior space is an effective way in the context of adding a traditional touch to the modern interior space. Meanwhile, in the process of applying Korean traditional lattices to modern interior space, some problems occurred: it has not been properly embodied; or it has been mistaken for those of China and Japan sharing similar culture with Korea. Thus, this study is designed to have precise knowledge of Korea, Chinese and Japanese traditional lattices. Besides, typical patterns of each nation's lattices are comparatively analyzed from the geometrical viewpoint, in a bid to pave the way for th modernization of traditional lattices. In particular, standards are set and proposed to identify different nations's lattices mainly focusing on grid type lattices which are often used and confusing.

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Effects of Climatic Factors varied due to the Type of Plastic House, Cultural Season and Locations in the Plastic House on the Growth of Cucumber Plants Grown in Rockwool (Plastic house의 형태, 재배양식 및 시설내 위치에 따른 기상환경의 차이가 암면재배 오이의 생장에 미치는 영향)

  • Lim Jung-Mook;Kwon Byung-Sun;Shin Dong-Young;Hyun Kyu-Hwan;Kim Hak-Jin;Chung Soon-Ju;Lee Beom-Seon;Lim June-Taeg
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.218-222
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    • 2006
  • This experiment was conducted to investigate the effects of climatic factors varied due to the type of plastic house, cultural season and location in the house on the growth of cucumber plants grown by nutrient solution. There were two growing periods, summer culture and retarding culture, two types of plastic houses, 1-2W type house and post-less house. Air temperature, relative humidity and amount of solar radiation in the plastic houses were measured. Also, dry weight of leaves and stems, plant height, number of leaves per plant, leaf area per plant and fresh weight of fruits per plant were observed. Plant growth analysis were conducted and interrelationships between climatic factors and physiological characteristics were investigated. The results were as follows. There were no differences between the type of plastic houses in the average air temperature and average relative humidity in the plastic house, but amount of solar radiation in 1-2W type house was significantly higher than that of postless house. Daily cumulative solar radiation were highest in southwest side of 1-2W type house and northwest side of postless house. Plant height and number of leaves per plant were higher in summer culture than retarding culture, while leaf area per plant was higher in retarding culture than summer culture. Relative growth rate (RGR) showed highly significantly positive correlations with net assimilation rate (WAR) and leaf area ratio (LAR). Contribution of NAR to RGR was much higher than that of LAR. Crop growth rate (CGR) showed highly significantly positive correlations with leaf area index (LAI). It appeared that increase of LAI was important to increase productivity of cucumber. Average daily air temperature for the whole growing period showed highly significantly positive correlations with RGR and NAR. Furthermore, cumulative solar radiation for the whole growing period in retarded culture showed significantly positive correlation with RGR and NAR.

A Study of Impulse Buying and Psychological Characteristics of College Women by Their Clothing Shopping Orientation (쇼핑성향에 따른 여대생의 충동구매와 심리적 특성에 관한 연구)

  • 강경자
    • The Research Journal of the Costume Culture
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    • v.7 no.4
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    • pp.127-138
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    • 1999
  • The college students selected by random sampling were classified into several groups by their impulse buying pattern and the psychological characteristics that cause them. Their life styles, fashion leadership, self-confidence and self-image in clothing were the subjects of comparative analysis. The results of this research can be summarized as follows. 1. The college women students could be classified into several groups by their impulse buying orientations. 2. The student life styles were different by their shopping orientations. The high shopping involved shopping type and the leisure pursuit shopping type attached great importance to material life and modern life style. The economic shopping type took the intellectual self-image seriously, and the low shopping involved type thought much of the traditional life style. 3. The high shopping involved type and the leisure pursuit shopping type were more impulse buying oriented. They showed the tendencies to the fashion innovativeness and fashion opinion leadership. But the economic shopping involved shopping type and low shopping involved shopping type showed quite different tendencies. The high shopping involved shopping type and low shopping involved shopping type were contrast in their self-confidence in shopping. The former showed the highest self-confidence and the latter showed the lowest self-confidence. 4. The high shopping involved shopping type were more extroversive, dominant, sensitive and pioneering. These psychological characteristics were supposed to contribute to their impulse buying. 5. The high shopping involved shopping type and the leisure pursuit shopping type perceived themselves as more sophisticated, modern, creative and sociable. They thought they had diverse life styles. These two groups were more impulse buying oriented, and their self-images were different from economic shopping type and low shopping involved shopping type.

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Spiritual Health in Korean Culture -Q methodological approach- (한국인의 영적건강에 관한 유형별 탐색 - Q 방법론적 접근 -)

  • Shim, Hyung Wha
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.22 no.2
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    • pp.129-138
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    • 2015
  • Purpose: This paper is a basic study done to establish spiritual health concepts according to Korean culture. The focus was on identifying perceived concepts of spiritual health in Korean people. Methods: The Q method, which is effective in measuring individual subjectivity, was used in this study. Results: Perception of the concept of spiritual health was found to have 3 independent types, Type I is a self-directed, present life centered type. This is a group stressing the importance of planning self-directed life and the people of this group consider the values of the present life as important. Type II is a faith-oriented, afterlife centered type. This is a group whose essence of spiritual health is religious devotion like faith in god and the people of this group do not sympathize with the oriental world view. Type III is an oriental, value-sharing type. This is a group with an oriental world view and the people of this group stress importance in sharing values with communities or others. Conclusion: These results not only become the basis for understanding the concept of spiritual health among Koreans, but also suggest the necessity of comprehensive education for spiritual health promotion.

A Study on the Head Type of Korean Women's for Headgear Pattern Making (모자 제작을 위한 여자 머리 형태에 관한 연구 -유형별 머리모형 개발을 중심으로-)

  • Ahn Young-Sill;Suh Mi-A
    • The Research Journal of the Costume Culture
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    • v.12 no.6 s.53
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    • pp.1021-1030
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    • 2004
  • The purpose of this study was to develop and make the head form by setting the region of the head for detailed measurement of the head to make the type of closely fit hat and typifying the head form of Korean women's with each region of the head based on the measurements of each region of the head according characteristics. A total of 285 Korean women's in the age group of 18 to 35 years old. It were measured through the direct measurement method by selecting 67 measurement items. As a result of the findings obtained from cluster analysis could be classified into 3 type. Type 1($46\%$) is U-shaped form, that is high in head height on sides and circumference is smallest, whose facial region and occiput is flattest of three types. Type 2($22.4\%$) is inverted triangle form, that is flat in head ridge, low in head height on sides and wide in the frontal width. Type 3($31.6\%$) is oval form, that is largest in size, has the projected occiput and is of three-dimensional form.

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Classification and Characteristics of the Body Shape for Early Adolescent Boys (청소년 전기 남학생의 체형 유형화 및 유형별 체형 특성에 관한 연구)

  • Kim Kyung-A;Suh Mi-A
    • The Research Journal of the Costume Culture
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    • v.13 no.3 s.56
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    • pp.344-360
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    • 2005
  • The purpose of the study is to identify the physical characteristics of early adolescent boys, to classify body shapes by physical characteristic. The subjects were 549 boys in the capital area. Their body shapes were identified and classified based on 47 anthropometric measurements, 43 photographic measurements and 10 indexed measurements. For data analysis were performed descriptive statistics, factor analysis, cluster analysis, ANOVA and Duncan test using SPSS Ver. 10. According to the result of extracting factors indicating the characteristics of body shape, horizontal size, vertical length, lateral posture, the lateral shape of the abdomen and the hip, the shape of the back protrusion, the front shape of the trunk and was the shape of the shoulders. According to the result of classifying body shapes, four types of shape - T(Tall) type, P(Petite) type, L(Large) type and R(Regular) type were identified. The results of this study are expected to contribute to planning sizes according to the type of body shape and improving the fitness of ready-made clothes in apparel and school uniform manufacturers.

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