• 제목/요약/키워드: Culture collection

검색결과 895건 처리시간 0.026초

A New Pseudoxanthomonas Species, Pseudoxanthomonas suwonensis sp. Nov

  • Cha, In-Bae;Weon, Hang-Yeon;Kim, Byung-Yong;Kim, Jong-Shik;Lee, Seon-Young;Go, Seung-Joo;Hong, Seung-Beom;Im, Wan-Taek;Kwon, Soon-Wo
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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    • pp.187.2-187
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    • 2005
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Distribution and in vitro Fruiting of Cordyceps militaris in Korea

  • Shrestha, Bhushan;Han, Sang-Kuk;Lee, Won-Ho;Choi, Seong-Keun;Lee, Je-O;Sung, Jae-Mo
    • Mycobiology
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    • 제33권4호
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    • pp.178-181
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    • 2005
  • Cordyceps militaris specimens were continuously collected by Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University from different mountains, national parks and recreation parks of Korea from 1986 to 2002, mainly from late May to October of each year. Dry specimens of C. militaris along with their isolates have been preserved in EFCC. Fruiting of C. militaris was induced from single ascospore isolates as well as their combinations in brown rice medium. Fruiting experiments showed that combinations of single ascospore isolates produced fertile fruiting bodies, but single isolates could not produce any fruiting bodies. It was shown that two isolates of the opposite mating types were required to produce fertile stromata. However, combinations of the same mating type isolates produced no fruiting body, showing that C. militaris is a bipolar, heterothallic fungus.

Effect of Preservation Periods and Subcultures on Fruiting Body Formation of Cordyceps militaris In Vitro

  • Sung, Jae-Mo;Park, Young-Jin;Lee, Je-O;Han, Sang-Kuk;Lee, Won-Ho;Choi, Sung-Keun;Shrestha, Bhushan
    • Mycobiology
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    • 제34권4호
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    • pp.196-199
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    • 2006
  • Effects of various preservation periods and subcultures on fruiting body formation of Cordyceps militaris were investigated using EFCC C-10995 single ascospore strains. Fruiting body formation by original strains was profuse when preserved at $4^{\circ}C$ for $5{\sim}6$ months. Fruiting from subcultures was stable till second to sixth subcultures, after which it decreased sharply. The more the colony color of subcultures changed, the less the fruiting bodies formed. Liquid inoculum preparation of single ascospore strains in the same or separate broths did not affect fruiting body formation. Similarly, two strains C-10995-3 and C-10995-6 in different numbers during liquid inoculum preparation produced similar fruiting bodies.

폴리프로필렌 스트로를 이용한 곰팡이의 액체질소 보존 (Preservation of Fungi in Liquid Nitrogen Using Polypropylene Straws)

  • 전영아;신명숙;김효진;김대호;고승주;홍승범
    • 한국균학회지
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    • 제34권1호
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    • pp.54-58
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    • 2006
  • 액체질소 보존법은 동결 건조 보존이 불가능한 것을 포함한 곰팡이를 가장 효과적으로 보존할 수 있는 방법 중의 하나로서, 유전적인 변화와 오염을 방지하고 장기보존이 가능한 이점을 가진다. 크료튜브 대신 액체질소 보존에 사용할 수 있는 폴리프로필렌 스트로는 경제성, 안전성, 편리성 그리고 공간 이용성 등의 이점을 가진다. 이에 한국농업미생물자원센터(KACC)에서는 폴리프로필렌 스트로를 이용한 곰팡이의 액체질소 보존법을 정립하였으며, 이를 상세하게 소개하였다.

Re-Identification of Aspergillus Subgenus Circumdati Strains in Korea Led to the Discovery of Three Unrecorded Species

  • Anbazhagan Mageswari;Yunhee Choi;Le Dinh Thao;Daseul Lee;Dong-Hyun Kim;Myung Soo Park;Seung-Beom Hong
    • Mycobiology
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    • 제51권5호
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    • pp.288-299
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    • 2023
  • Aspergillus is one of the largest and diverse genera of fungi with huge economical, biotechnological, and social significance. Taxonomically, Aspergillus is divided into six subgenera comprising 27 sections. In this study, 235 strains of Aspergillus subgenus Circumdati (section: Candidi, Circumdati, Flavi, Flavipedes, Nigri, and Terrei) preserved at the Korean Agricultural Culture Collection (KACC) were analyzed and re-identified using a combined dataset of partial b-tubulin (BenA), Calmodulin (CaM) gene sequences and morphological data. We confirmed nineteen species to be priorly reported in Korea (A. neotritici, A. terreus, A. floccosus, A. allahabadii, A. steynii, A. westerdijkiae, A. ochraceus, A. ostianus, A. sclerotiorum, A. luchuensis, A. tubingensis, A. niger, A. welwitschiae, A. japonicus, A. nomius, A. tamarii, A. parasiticus, A. flavi, and A. oryzae). Among the studied strains, three species (A. subalbidus, A. iizukae, and A. uvarum), previously unreported or not officially documented, were discovered in Korea, to the best of our knowledge. We have given a detailed description of the characteristic features of the three species, which remain uncharted in Korea.

Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants

  • Le Dinh Thao;Hyorim Choi;Yunhee, Choi;Anbazhagan Mageswari;Daseul Lee;Dong-Hyun Kim;Hyeon-Dong Shin;Hyowon Choi;Ho-Jong Ju;Seung-Beom Hong
    • The Plant Pathology Journal
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    • 제40권1호
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    • pp.16-29
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    • 2024
  • The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.