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http://dx.doi.org/10.4489/KJM.2006.34.1.054

Preservation of Fungi in Liquid Nitrogen Using Polypropylene Straws  

Jeon, Young-Ah (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Shin, Myung-Sook (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Kim, Hyo-Jin (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Kim, Dae-Ho (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Go, Seung-Joo (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Hong, Seung-Beom (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
Publication Information
The Korean Journal of Mycology / v.34, no.1, 2006 , pp. 54-58 More about this Journal
Abstract
Liquid nitrogen storage is the most effective way to preserve many fungi including what cannot be lyophilized. The use of polypropylene straws instead of cryotubes has many advantages in economy, safety, convenience, and space-saving. We, Korean Agricultural Culture Collection (KACC), established the fungal preservation methods in liquid nitrogen using polypropylene drinking straws and introduced the methods in detail.
Keywords
Fungi; Liquid nitrogen storage; Long-term preservation; Polypropylene straw;
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  • Reference
1 Hwang, S.-W. 1968. Investigation of ultra-low temperature for fungal cultures. I. Evaluation of liquid-nitrogen storage for preservation of selected fungal cultures. Mycologia 60: 613-621   DOI
2 Butterfield, W., Jong, S. C. and Alexander, M. J. 1974. Preservation of living fungi pathogenic for man and animals. Can. J. Microbiol. 20: 1665-1673   DOI   ScienceOn
3 Hwang, S.-W, Kwolek, W. F. and Haynes, W. C. 1976. Investigation of ultra-low temperature for fungal cultures. III. Viability and growth rate of mycelial cultures following cryogenic storage. Mycologia 68: 377-387   DOI
4 Challen, M. P. and Elliott, T. J. 1986. Polypropylene straw ampoules for the storage in microorganisms in liquid nitrogen. J. Microbiol. Methods 5: 11-23   DOI   ScienceOn
5 Dietz, A. 1975. Nitrogen preservation of stock cultures of unicellular and filamentous microorganisms. Pp 22-26. In: Rinfret, A. P. and La Salle, A. B. Eds. Round table conference on cryogenic preservation of cell cultures. National Academy of Science, Washington DC
6 Harrison, A. P. 1995. Survival of bacteria upon repeated freezing and thawing. J. Bacteriol. 70: 711-715
7 Henry, J. and Kirsop, B. 1989. Cryopreservation of yeasts in polypropylene straws. World Federation for Culture Collection Technical Information Sheet No.3
8 Hoffmann, P. 1989. Cryopreservation of fungi. World Federation for Culture Collection - Technical Information Sheet No.5
9 Hwang, S.- W. 1966. Long-term preservation of fungus cultures with liquid nitrogen refrigeration. Appl. Microbiol. 14: 784-788
10 Nakasone, K. K., Peterson, S. W. and Jong, S. C. 2004. Preservation and distribution of fungal cultures. Pp 37-47. In: Mueller G M., Bills, G F. and Foster, M. S. Eds. Biodiversity of fungi - Inventory and monitoring methods. Elsevier Academic Press, San Diego
11 Ohmasa, M., Abe, Y., Babasaki, K., Hiraide, M. and Okabe, K. 1992. Preservation of cultures of mushrooms by freezing. Trans. Mycol. Soc. Jap. 33: 467-479
12 Smith, D. 1983. Cryoprotectants and the cryopreservation of fungi. Trans. Brit. Mycol. Soc. 80: 360-363   DOI
13 Smith, D. 1991. Maintenance of filamentous fungi. Pp 133-159. In: Kirsop, B. E. and Doyle, A. Eds. Maintenance of microorganisms and cultured cells - A manual of laboratory methods. Academic Press, San Diego
14 Staplers, J. A., de Hoog, A. and Vlug, I. J. 1987. Improvement of the straw technique for the preservation of fungi in liquid nitrogen. Mycologia 79: 82-89   DOI
15 Tuite, J. 1968. Liquid nitrogen storage of fungi sealed in a polyester film. Mycologia 60: 591-594   DOI
16 Simione, F. P., Daggett, P. M., McGrath, M. S. and Alexander, M. T. 1977. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502   DOI   ScienceOn
17 Elliot, T. J. 1976. Alternative ampoule for storing fungal cultures in liquid nitrogen. Trans. Brit. Mycol. Soc. 67: 545-546   DOI