• Title/Summary/Keyword: Culture center

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Effects of Insulin, Transferrin and Selenium (ITS) on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

  • Quan, Yan-Shi;Naruse, Kenji;Kim, Baek-Chul;Kim, Hong-Rye;Han, Rang-Xun;Choi, Su-Min;Park, Chang-Sik;Jin, Dong-Il
    • Reproductive and Developmental Biology
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    • v.31 no.4
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    • pp.261-265
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    • 2007
  • Insulin, transferrin and selenium (ITS) complex is reported to improve in vitro development of oocytes and embryos. This study was carried out to investigate the effects of ITS during in vitro culture (IVC) of porcine parthenogenetic and nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. The electrically activated oocytes were cultured in Porcine Zygote Medium (PZM-3) with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 7 days. Also, the electrically activated reconstructed embryos were cultured in PZM-3 with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 6 days. Addition of ITS to culture medium did not affect development of porcine parthenogenetic embryos in vitro. To test the effect of ITS on the in vitro development of porcine NT embryos, factorial experiments were also performed for in vitro maturation (IVM) medium (TCM-199) with or without 1% ITS and culture medium (PZM-3) with or without 0.5% ITS. Addition of 0.5% ITS to culture medium increased (p<0.05) the proportion of NT blastocysts compared with non-treated group. In contrast, addition of 1% ITS to culture medium was ineffective or had a detrimental effect. Also, addition of ITS only to maturation medium increased (p<0.05) the percentage of NT blastocysts formation compared with the control group. In conclusion, addition of ITS to IVM or IVC medium could improve subsequent blastocyst development of porcine NT embryos.

Optimization of Culture Conditions and Continuous Production of Chitosan by the Fungi, Absidia coerulea

  • Kim, Woo-Jun;Lee, Woo-Gi;Kalaimahan Theodore;Chang, Ho-Nam
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.1
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    • pp.6-10
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    • 2001
  • The production of chitosan from the mycelia of Absidia coerulea was studied to improve cell growth and chitosan productivity. Culture conditions were optimized in batch cultivation (pH 4.5, agitator speed of 250 rpm, and aeration rate of 2 vvm) and the maximum chitosan concentration achieved was 2.3g/L under optimized conditions. Continuous culture was carried out successfully by the formation of new growth spots under optimized conditions, with a chitosan productivity of 0.052g/L(sup)-1 h(sup)-1, which is the highest value to date, and was obtained at a dulution rate of 0.05h(sup)-1. Cell chitosan concentrations reached about 14% in the steady state, which is similar to that achieved in batch culture. This study shows that for the continuous culture of Absidia coerulea it is vital to control the medium composition.

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THE NATURE OF SAFETY CULTURE: A SURVEY OF THE STATE-OF-THE-ART AND PROMOTING A POSITIVE SAFETY CULTURE

  • Choudhry M. Rafiq;Fang Dongping
    • International conference on construction engineering and project management
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    • 2005.10a
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    • pp.480-485
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    • 2005
  • This paper reviews the literature on safety culture focusing particularly on research carried out from 1998 onwards. The term 'safety culture' is clarified as it is typically applied to organizations, to safety and particularly to construction safety. Some clarifications in terms of levels of aggregation, positive safety culture and safety performance are provided by presenting appropriate empirical evidences and their theoretical developments. Safety culture is a subset of organizational culture that is thought to influence employees' attitudes and behavior in relation to an organization's ongoing health and safety performance. Implications for future research in the area are addressed, as safety culture has in recent years become the focus of much attention in all industries, and in the construction industry in particular.

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A Novel Claw Pole Eddy Current Load for Testing a DC Counter Rotating Motor Part I: Construction

  • Kanzi, Khalil;Dehafarin, Abolfazl;Roozbehani, Sam;Kanzi, Majid;Vasheghani, Qasem
    • Journal of international Conference on Electrical Machines and Systems
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    • v.1 no.3
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    • pp.321-329
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    • 2012
  • Providing variable load for testing a motor in high speed conditions is usually a difficult task. The eddy current brake can be used in application of load testing of motors. This paper deals with construction of a novel claw pole eddy current brake which is employed as a load for a DC counter rotating motor (CRM). These kinds of motors have two inner and outer shafts that rotate in opposite directions simultaneously, which are particularly suitable for under water propulsion systems. The prototype 45KW eddy current brake consists of two parts. One of them is installed on the inner shaft of the 60KW DC CRM and the other one is installed on its outer shaft. The simulation and experimental results with prototype brakes are also analyzed by using MATLAB/Simulink and the operational characteristic of the brake is demonstrated as a function of the motor speed and current of the magnetic poles.

Efficiency Analysis for the Community Culture Center in Capital region by DEA (DEA에 의한 수도권 소재 문화예술회관의 효율성 분석)

  • Joo, Hi-Yeob;Kim, Seung-Mo
    • The Journal of the Korea Contents Association
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    • v.12 no.3
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    • pp.181-189
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    • 2012
  • This research focus on Community Culture Center's efficiency according to the regional and operating subject in Capital region. Recently the importance and potential market of cultural industry is growing. In addition, as a basis for local cultural industries, the importance of Community Culture Center has been highlighted. Therefore, for the activation of cultural industry, Community Culture Center on the efficiency of the operation should be reconsidered. To analyze Community Culture Center, using DEA, the efficiency of public organizations tool, which focus on operational efficiency. Depending on the results of analysis, Seoul, Incheon and Gyeonggi province was able to confirm their efficiency. In addition, according to the Direct Operating, Industrial Operating, Foundation, Private Consignment Agency their efficiency showed significant results. Looking at regional Community Cultural Centers in Seoul and Gyeonggi Province will see that two are operating efficiently. On the other hand, the Community Cultural Center in Incheon was not efficiency. Looking at the operating entity basis, Direct Operating Agencies and Industrial Operating Agencies were operating efficiently. On the other hand, Foundation Act of agencies and Private Consignment Agencies have been operating relatively inefficiently.

A Benzenoid from the Stem of Acanthopanax senticosus

  • Ryu, Ji-Young;Son, Dong-Wook;Kang, Jung-Il;Kim, Hyun-Su;Kim, Bak-Kwang;Lee, Sang-Hyun
    • Archives of Pharmacal Research
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    • v.27 no.9
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    • pp.912-914
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    • 2004
  • Seven compounds were isolated from the stem of Acanthopanax senticosus by repeated col-umn chromatography. Their structures were elucidated as isovanillin (1), (-)-sesamin (2), iso-fraxidin (3), (+)-syringaresinol (4), 5-hydroxymethylfurfural (5), eleutheroside B (6), and eleuth-eroside E (7) by spectral analysis. Among them, isovanillin (1) was isolated for the first time from the family Araliaceae.

Effects of Dietary Yeast Culture Containing Recombinant Porcine Somatotropin on Growth Performances in Broiler Chickens (Recombinant Porcine Somatotropin 함유 Yeast Culture의 사료 내 첨가가 육계의 생산성에 미치는 영향)

  • Ko Y. M.;Kim D. W.;Kim K. E.;Shin S. C.;You S. J.;Ahn B. K.;Kang C. W.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.245-253
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    • 2004
  • This study was conducted to investigate the effects of dietary yeast culture containing rPST on growth performances and the characteristics of body compositions in broiler chickens. A total of 460 Ross male broiler chicks aged 2-day-old were fed one of five experimental diets; Control(devoid of all), TI (with $0.1\%$ antibiotics; chlorotetracycline), T2 (with $0.1\%$ rPST-yeast culture), T3 (with $0.2\%$ rPST-yeast culture) or T4 (with $0.2\%$ SC yeast culture) for 6 weeks. Feed consumption and body weights were measured weekly. At week 5 of the experiment, 10 chicks were randomly selected and sacrificed. The relative weights of each organ and the chemical composition of edible meat were measured. The enzyme activity, total cholesterol, Ca and P were also determined. Tibial weight, bone strength and chemical composition were investigated. There were no significant differences in feed intake and feed conversion ratios among the treatments throughout the experimental period. Body weight gains tended to be increased by feeding of diets containing rPST-yeast culture. The relative weight of breast muscle in T3 group was significantly higher than that of the control (P<0.05). The moisture contents of breast meat in groups fed diets containing rPST-yeast culture or SC yeast culture were significantly increased as compared with those of the control and n. However, the contents of crude protein and ether extract were not affected by feeding of rPST-yeast culture. There were no significant differences in GOT, total cholesterol, Ca and P. The relative weight and strength and proximal composition of the tibia were also not affected by dietary treatments. These results indicated that dietary rPST-yeast culture may be a valuable alternative for optimizing growth performances, particularly for improving the yield of breast muscle.

Development and Evaluation of the Educational Program for South Korean Middle School Students in Preparation for Reunification on North Korean Family Living Culture (중학생을 위한 통일대비 가정생활문화교육 프로그램 개발과 평가)

  • Lee, Hana;Kim, Yookyung;Song, Jieun;An, Soon-Hee;Lee, Yonsuk;Lee, Yhe-Young;Lee, Yoon-Jung;Lim, JungHa;Jung, Min-Young;Chung, SoonHwa;Han, Youngsun
    • Journal of Korean Home Economics Education Association
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    • v.29 no.3
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    • pp.105-124
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    • 2017
  • This study aimed at developing an educational program for South Korean adolescents on North Korean family living culture so that the students become familiar with the values and everyday lifestyles of North Korea. Understanding of North Korean culture is considered important as a foundation for social integration in prospect of future reunification. The contents of the program were developed based on the analysis of North Korean defectors' interviews and review of the literature and media reports on North Korean family culture. The program consists of 12 units of 40-minute sessions with the following topics: economy and consumption, leisure activities, family relationships, dating and spouse choices, fashion changes, clothing acquisition and care, food choices and local diets, dietary problems, housing, and neighborhood. The program was implemented between December 21 and 30, 2015 in a middle school located in Seoul. This program is expected to be useful in preparing the students as future leaders to create harmonious family living culture in the reunified Korea.

A study on the Administration of School Library Media Center as a culture spade for public education (공교육 정상화를 위한 문화적 공간으로서의 학교도서관 활용방안 연구)

  • 조혜영;김성희
    • Journal of Korean Library and Information Science Society
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    • v.35 no.2
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    • pp.351-370
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    • 2004
  • This paper starts by exploring the problems of public education under knowledge based society. It then analyzes the questionnaire about the function of school library media center as a culture space. The results show that school library media center should take a role as repertory appreciation center of various arts, recreation center, and interactive community.

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