• 제목/요약/키워드: Culture and System

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돼지 자궁내막 세포의 3차원 배양과 Plasminogen Activator 활성화 분석 (Analysis of Plasminogen Activators Activity and Three Dimensional (3D) Culture of Endometrial Cells in Pigs)

  • 차혜진;이상희;정희태;양부근;박춘근
    • 한국수정란이식학회지
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    • 제28권3호
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    • pp.273-280
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    • 2013
  • The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.

NONLINEAR IMPULSIVE SYSTEM OF MICROBIAL PRODUCTION IN FED-BATCH CULTURE AND ITS OPTIMAL CONTROL

  • GAO CAIXIA;LANG YANHUAI;FENG ENMIN;XIU ZHILONG
    • Journal of applied mathematics & informatics
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    • 제19권1_2호
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    • pp.203-214
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    • 2005
  • In this study the optimal control of fed-batch glycerol fermentation is investigated based on an impulsive dynamical system. Considering the sudden increase of the glycerol and alkali in fed-batch culture of biodissimilation of glycerol to 1,3-propanediol, this paper proposes a non-linear impulsive system of fed-batch culture. The existence, uniqueness and regularity properties of piecewise solution for the system are proved. In view of the controllability of volumes of glycerol added to the reactor instantaneously, the paper constructs an optimal control model based on the nonlinear impulsive system and the existence of the optimal control is obtained. The control variables here are the moments and the sizes of jumps in the states at the discrete instants and the objective is to maximize the productivity of 1,3-propanediol over one cycle.

요양병원 의료기관인증제 시행 후 환자안전, 의료의 질, 조직문화 및 병원경영성과의 변화 (Change in patient safety, quality of medical care, organization culture and hospital management performance after healthcare accreditation system for convalescent hospital)

  • 서영호;김건엽;김명선
    • 한국산학기술학회논문지
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    • 제19권9호
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    • pp.391-401
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    • 2018
  • 이 연구는 요양병원 의료기관인증제 시행 후 요양병원의 환자안전, 의료의 질, 조직문화 및 경영성과에 미친 영향을 파악함에 목적이 있다. 2015년 9월 말까지 요양병원 의료기관인증을 획득한 대구지역의 36개 요양병원 중 10개 기관을 임의로 선정하였으며 2016년 2월 15일부터 2016년 3월 25일까지 요양병원 종사자들에게 설문조사를 하여 253명을 최종분석 대상자로 선정하였다. 설문조사 내용으로는 일반적 특성, 인증이후 환자안전과 의료의 질 향상, 조직문화, 경영성과의 변화가 포함되어 있다. 연구결과 환자안전과 의료의 질 향상의 변화정도에서는 진료지원체계가 3.93점으로 가장 높았으며, 조직문화의 변화정도에서는 인적자원문화가 3.78점으로 가장 높았고, 요양병원의 경영성과 부분에서는 내부프로세스 관점, 학습 및 성장관점이 3.71점으로 가장 높게 나타났다. 병원경영성과에 영향을 미치는 요인을 파악한 결과 직종이 행정직보다 간호직, 직급이 국장보다 사원이거나 과장, 환자안전과 질 향상에서 기본가치체계가 개선되었다고 생각하는 경우, 조직문화에서 인적자원문화, 개방체계문화, 위계서열문화, 생산중심문화가 개선되었다고 생각하는 경우 병원경영성과를 긍정적으로 변화시키는 영향 요인이라 응답하였다. 요양병원 의료기관인증제 시행 후 환자안전과 의료의 질 향상에는 높은 변화를 보여 긍정적 영향을 미쳤으나, 조직문화의 4가지 유형 중 위계서열문화와 생산중심문화는 보통수준의 변화를 보였으며 병원경영성과 중 재무관점은 다른 관점에 비해 상대적으로 낮은 변화를 보였다.

문화원형복원교육 키오스크시스템 설계 및 구현 (Design and Implementation of Kiosk System for Culture Heritage Restoration Education)

  • 유인화;박선희;전병호
    • 한국콘텐츠학회:학술대회논문집
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    • 한국콘텐츠학회 2004년도 춘계 종합학술대회 논문집
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    • pp.188-192
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    • 2004
  • 발달된 문화유산을 많이 가지고 있었으면서도 디지털 컨텐츠로 제작되어진 역사 문화와 관련한 정보가 많이 부족하므로 문화원형복원 과정을 상세히 제공하여 역사 문화를 이해하는데 도움을 주는 시스템이 필요하다. 단순한 지식의 형태로 접근하고 있는 문화유산에 대해 문화유산의 중요성을 알리고 누구나 쉽게 접근하고, 인지하며, 학습할 수 있는 교육 과정을 구성하였다. 이 교육과정에 따라 문화원형복원 키오스크 시스템을 구현하였다.

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Regulatory Oversight of Nuclear Safety Culture and the Validation Study on the Oversight Model Components

  • Choi, Young Sung;Jung, Su Jin;Chung, Yun Hyung
    • 대한인간공학회지
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    • 제35권4호
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    • pp.263-275
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    • 2016
  • Objective: This paper introduces the regulatory oversight approaches and issues to consider in the course of safety culture oversight model development in the nuclear field. Common understanding on regulatory oversight and present practices of international communities are briefly reviewed. The nuclear safety culture oversight model of Korea is explained focusing on the development of safety culture definition and components, and their basic meanings. Oversight components are identified to represent the multiple human and organizational elements which can affect and reinforce elements of defense in depth system for nuclear safety. Result of validation study on safety culture components is briefly introduced too. Finally, the results of the application of the model are presented to show its effectiveness and feasibility. Background: The oversight of nuclear licensee's safety culture has been an important regulatory issue in the international community of nuclear safety regulation. Concurrent with the significant events that started to occur in the early 2000s and that had implications about safety culture of the operating organizations, it has been natural for regulators to pay attention to appropriate methods and even philosophy for intervening the licensee's safety culture. Although safety culture has been emphasized for last 30 years as a prerequisite to ensure high level of nuclear safety, it has not been of regulatory scope and has a unique dilemma between external oversight and the voluntary nature of culture. Safety culture oversight is a new regulatory challenge that needs to be approached taking into consideration of the uncontrollable aspects of cultural changes and the impacts on licensee's safety culture. Although researchers and industrial practitioners still struggle with measuring, evaluating, managing and changing safety culture, it was recognized that efforts to observe and influence licensees' safety culture should not be delayed. Method: Safety culture components which regulatory oversight will have to focus on are developed by benchmarking the concept of physical barriers and introducing the defense in depth philosophy into organizational system. Therefore, this paper begins with review of international regulatory oversight approaches and issues associated with the regulatory oversight of safety culture, followed by the development of oversight model. The validity of the model was verified by statistical analysis with the survey result obtained from survey administration to NPP employees in Korea. The developed safety culture oversight model and components were used in the "safety culture inspection" activities of the Korean regulatory body. Results: The developed safety culture model was confirmed to be valid in terms of content, construct and criterion validity. And the actual applicability in the nuclear operating organization was verified after series of pilot "safety culture inspection" activities. Conclusion: The application of the nuclear safety culture oversight model to operating organization of NPPs showed promising results for regulatory tools required for the organizations to improve their safety culture. Application: The developed oversight model and components might be used in the inspection activities and regulatory oversight of NPP operating organization's safety culture.

향토문화 콘텐츠를 위한 디지털 편찬 관리시스템 개발에 관한 연구: "한국향토문화전자대전"의 사례를 중심으로 (A Study on Development of Digital Compilation Management System for Local Culture Contents: Focusing on the Case of The Encyclopedia of Korean Local Culture)

  • 김수영
    • 정보관리학회지
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    • 제26권3호
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    • pp.213-237
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    • 2009
  • 향토문화란 한 지방의 자연환경 속에서 과거로부터 현재까지 면면히 전승되어 온 역사와 전통, 풍물과 생활, 예술과 유물 및 유적 등의 모든 유산을 의미한다. 한국학중앙연구원에서는 이런 향토문화를 디지털 콘텐츠로 제작하고 이를 이용하여 "한국향토문화전자대전"을 편찬하였다. 향토문화 콘텐츠는 기록물의 특성을 가지고 있어 출처주의, 계층목록과 같은 기록물관리체계를 따르고 있으며 이를 편찬 관리하기 위한 시스템은 기초자료, 단편적 정보 및 고급정보 등이 하나의 시스템 안에서 순환하면서 새로운 지식정보를 만들어내도록 도와주는 순환형 지식정보관리시스템을 지향한다. 순환형 지식정보관리시스템의 이용자는 이 시스템을 통해 직접적으로 자료를 수집할 수 있을 뿐만 아니라 다른 곳으로부터 데이터를 수집할 수 있으며, 나아가 수집한 데이터를 가공하여 새로운 지식 정보를 창출할 수 있다. 그러나 다양한 향토문화 콘텐츠의 구조에 포함된 의미적인 특징을 손상시키지 않고 데이터베이스를 구축하기가 매우 어렵고, 또한 이러한 작업은 장시간에 걸쳐 여러 차례의 교정 작업이 수행되어야 하므로 문서편찬, 교정, 서비스가 동시에 수행되는 시스템을 필요로 한다. 따라서 본 논문에서는 "한국향토문화전자대전"의 사례를 중심으로 고문서가 많이 포함된 향토문화 콘텐츠의 의미적인 특징을 손상시키지 않으면서, 문서의 구조정보를 표현할 수 있는 XML 기반의 디지털 편찬 관리시스템을 제시하고 본 연구에서 개발된 관리시스템에서 향토문화 콘텐츠 관리를 위해 확장된 기능을 소개한다.

단체급식소 영양사의 종합적 품질경영(TQM) 수행과 조직문화와의 관계 규명에 관한 연구 (Relationship between TQM Performance and Organizational Culture of Dietitians in Institutional Foodservice)

  • 조기원;서의훈;윤지영
    • 한국식생활문화학회지
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    • 제22권2호
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    • pp.191-200
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    • 2007
  • This study was designed to investigate the correlation between perceived TQM performance and organizational culture of dietitians. The objective of the study is to help the management of foodservice by providing a direction which will elevate perceived TQM performance of dietitians and presenting plans which will ensure effective dietitians. Questionnaires handed out to 308 dietitians worked in institutional foodservice operation including elementary, middle and high schools, hospitals, business and industries. In terms of TQM importance and performance, the more important dietitians perceived, the higher their performance level rose. Data form the IPA, external and internal customer satisfaction, executive ability, communication system and technology, information technology and application ability, food process, strategy, and leadership were required further improvement by dietitians. Of present organizational culture model, human relations model and open systems model were more likely to be adopted by dietitians in middle and high schools. According to the type of foodservice management, the open systems model was more preferred by dietitians from self-operated operations rather than respondents from contracted operations. Canonical correlation analysis between TQM performance and organizational culture showed canonical correlation to be higher (canonical correlations coefficient: .66). In conclusion, TQM performance-organizational culture showed higher canonical correlation. In the organizational culture, foodservice operation is capable of improving the aims for the rational goal model and the open systems model. The results showed that TQM performance and organizational culture had significant relationship, especially positive organizational culture emphasizing on internal process and rational goal model would have influence on TQM performance of dietitians. Foodservice operation, however, should recognize importance of open and development culture to improve dietitians' TQM performance. To apply open system foodservice organization should encourage dietitian and foodservice employees to challenge and compete for the works, Moreover, organizational effort such as information exchange program and support system should be established.

대중과 과학기술: 이론적 흐름과 정책적 이슈 (Science in Public: Theoretical Trends and Policy Issues)

  • 송성수
    • 기술혁신학회지
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    • 제6권2호
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    • pp.137-158
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    • 2003
  • This paper examines theoretical trends and policy issues concerning science and public. The dominant perspective about it was changed from “popularization of science” to “public understanding of science (PUS)”. PUS pays attention to active roles of public in the understanding of science based on the contextual model. And recently various concepts are attempted to complement PUS such as “heterogeneous PUS”, “quantitative PUS”, “public understanding of research”, and “public participation in science”. PUS related activities in Korea can be analyzed using the concept of “science and technology culture system”. The characteristics of science and technology culture system in Korea can be summarized as follows: Its purpose is confined to support existing national innovation system; Its constituents have been unevenly and separately developed; Its material and institutional infrastructure is not sufficiently prepared; Its organizing mechanism is strongly influenced by government's execution power.

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태양열 시설원예 난방시스템 장기실증 성능분석 연구 (Study on the performance analysis of long-term field test for protected horticulture heating system using solar thermal energy)

  • 이상남;강용혁;유창균;김진수
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2005년도 춘계학술대회
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    • pp.403-407
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    • 2005
  • Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a greenhouse culture facility for reducing heating cost, increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex in Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely investigated by changing the control condition based on the temperature difference which is the most important operating parameter. For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, it is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

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태양열 시설원예 난방시스템 장기실증 성능분석 연구 (Study on the performance analysis of long-term field test for protected horticulture heating system using solar thermal energy)

  • 이상남;강용혁;유창균;김진수
    • 신재생에너지
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    • 제1권2호
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    • pp.53-59
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    • 2005
  • Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a green-house culture facility for reducing healing cost, Increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex In Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely Investigated by changing the control condition based on the temperature difference which Is the most important operating parameter For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, It is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

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