• Asian-Australasian Journal of Animal Sciences
• /
• 제31권6호
• /
• pp.827-834
• /
• 2018

Objective: The aim of this study was to compare the effects of $36.5^{\circ}C$ and $38.5^{\circ}C$ incubation temperatures on the maturation of bovine oocytes and developmental competence of embryos. Methods: In experiment 1, oocytes were maturated in bicarbonate-buffered TCM-199 for 22 hours in a humidified atmosphere of 5% $CO_2$ in the air at either $36.5^{\circ}C$ or $38.5^{\circ}C$ and nuclear maturation status were determined. In experiment 2, in vitro fertilized oocytes were allocated randomly into synthetic oviductal fluid medium with or without a mixture of 1 mM L-glutathione reduced and 1,500 IU superoxide dismutase and cultured in a humidified atmosphere of 5% $CO_2$, 5% $O_2$, and 90% $N_2$ in the air at $38.5^{\circ}C$ for 8 days. Results: There were no significant differences between incubation temperatures in terms of oocyte maturation parameters such as cumulus expansion, first polar body extrusion and nuclear maturation. Incubation temperatures during in vitro maturation had no effects on developmental competence of embryos, but supplementation of antioxidants increased (p<0.05) developmental competence of the embryos. Blastocysts from oocytes matured at $38.5^{\circ}C$ had comparatively higher inner cell mass, but low overall and trophectoderm cell numbers (p<0.05). Conclusion: The results of present study showed that maturation of bovine oocytes at $36.5^{\circ}C$ may provide a suitable thermal environment for nuclear maturation and subsequent embryo development.

• 한국패류학회지
• /
• 제32권1호
• /
• pp.37-43
• /
• 2016

This study was carried out to determine the optimal water temperature for the embryonic development and laboratory culture of larvae of an intertidal mud snail, Nassarius festivus. The embryos and hatched veliger larvae of N. festivus were incubated at six different temperatures (5, 10, 15, 20, 25 and $30^{\circ}C$). Developmental time for each stage decreased as water temperature increased. The elapsed time to develop to the veliger larva at 15, 20, 25 and $30^{\circ}C$ was 559, 155, 131 and 103 hrs, respectively. At 5 and $10^{\circ}C$, embryo developed to veliger larvae but failed to hatch out of the egg capsule. In contrast, all embryos successfully hatched in the temperature range from 15 to $30^{\circ}C$. The biological minimum temperature during the embryonic development of N. festivus was estimated to be $9.5{\pm}0.4^{\circ}C$. The cumulative water temperatures for blastula, gastrula and veliger stages were calculated as $111{\pm}84$, $486{\pm}185$, $1,164{\pm}72^{\circ}C$, respectively. Temperature also affected the larval survival. Five days after hatching, more than 84% of larvae survived at all experimental temperatures. However, survival began to decrease after 6 days. It was 0% at $30^{\circ}C$. Survival of larvae incubated for 8 days was higher at 15 and $20^{\circ}C$ than other experimental temperatures. We therefore suggest that the optimal range of temperature for embryonic development and larval survival of N. festivus is $15-20^{\circ}C$.

• 한국생물환경조절학회:학술대회논문집
• /
• 한국생물환경조절학회 1999년도 정기총회 및 학술논문발표요지
• /
• pp.75-78
• /
• 1999

토마토의 육묘시에 화아분화에 미치는 제요인의 영향에 대한 정확한 정보는 미흡하다. 흔히 육묘 중인 토마토의 생식생장을 촉진하기 위해서는 질소비료의 공급량을 줄이고 야간기온을 낮추어 야냉육묘해야 한다고 알려져 있다. 그러나 공정육묘기술의 도입으로 인하여 다품목 소량생산을 위주로 하는 국내의 채소 육묘농가들이 다양한 종류의 묘를 하나의 온실에서 생산해야 하는 한계성을 가지고 있다. (중략)

• Journal of the korean society of oceanography
• /
• 제31권3호
• /
• pp.117-122
• /
• 1996

Oxygen consumption by the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae) maintained in a culture tank for 7-10 weeks was measured with an automatic intermittent-flow-respirometer (AIFR). There was evidence for an endogenous tidal rhythm of 12.4 h in oxygen consumption, at irregular intervals of between about 7 and 18 hours during experiments of between 22 h 5 min and 70 h 32 min (duration) recording for fasting animals in constant darkness at constant temperatures in the absence of tidal rhythms.

• 대한생식의학회:학술대회논문집
• /
• 대한불임학회 2002년도 제43차 추계학술대회
• /
• pp.81.1-81.1
• /
• 2002

• 한국식생활문화학회지
• /
• 제31권3호
• /
• pp.243-249
• /
• 2016

The purpose of this study was to investigate the effects of Capsosiphon fulvescens powder on the functional and sensory characteristics of dumpling shell. Various dumpling shell samples were prepared with wheat flour along with the addition of different amounts of Capsosiphon fulvescens powder, and then instrumental characteristics and sensory evaluations were investigated. According to amylograph data, the composite Capsosiphon fulvescens powder-wheat flour samples had increased gelatinization temperatures with increasing Capsosiphon fulvescens powder content; whereas initial viscosity at $95^{\circ}C$, viscosity at $95^{\circ}C$ after 15minutes, and maximum viscosity were reduced. In terms of Hunter's color values, L, a and b values decreased with increasing Capsosiphon fulvescens powder content. Besides cooked weight, cooked volume and turbidity of dumpling shell increased as the addition level of Capsosiphon fulvescens powder increase. In terms of textural characteristics, addition of Capsosiphon fulvescens powder increased springiness, chewiness, brittleness, pringiness and adhesiveness. Based on sensory evaluations, the overall preference of dumpling shell with 3% added Capsosiphon fulvescens powder, was more effective than control.

• 한국식물병리학회지
• /
• 제10권3호
• /
• pp.215-221
• /
• 1994

Polygalacturonase (PG) produced by Botrytis cinerea in the culture broth containing citrus pectin as a carbon source was partially purified and characterized. PG was produced on a range of carbon sources such as starch, glycerol, cellobiose, and Na+-PAG with total activities of 34.8, 32.0, 29.2, 27.8 units, respectively. The specific activity was highest with 2316.7 units on Na+-PGA. Proteins of culture filtrate were concentrated with polyethylene glycol and acetone and applied to a hydroxyapatite column. Among three active fractions collected from the column, the reaction containing the highest PG activity was resolved by a Q-sepharose column. The active fraction from the Q-sepharose column was further purified by HPLC Mono Q column. The partially purified enzyme was analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Among a few protein bands revealed, the amount of the protein of which molecular weight estimated to be 43 kDa coincided with the PG activity. The partially purified PG had optimal temperatures between 35~55$^{\circ}C$ and pH between 4.5~5.5.

• Journal of Plant Biology
• /
• 제12권2호
• /
• pp.23-27
• /
• 1969

The promotive effect of Ca in semi-vitro culture of Oenothera biennis pollen was by and large identical to that of in vitro systems. This promotive effect became additive when boron was supplemented to the media and even more so if K was further supplemented. No such Ca action was observed then performed under the conditions of relatively low temperature. An uncoupling agent of ATP in respiration, DNP, inhibited the promotive action of B, but not that of Ca. The inhibitory effect of DNP was greater at low temperatures. IAA was rather inhibitory on pollen growth in semi-vitro culture, even much greater than it if DNP was supplemented to the IAA-containing media.

• 한국식생활문화학회지
• /
• 제10권3호
• /
• pp.167-174
• /
• 1995

A hazard analysis which included watching operations, measuring temperatures of foods throughout preparation and display, and sampling and testing for microorganisms of total plate counts and coliform bacteria was conducted in various phases of product flow of Naeng-myeun (Cold noodles) and Pi-bim bab (mixed rice) prepared at Korean restaurants. Large numbers of total plate counts were counted from the cooked foods after handling and holding. Ingestion of these foods must be considered high risk. Critical control points identified were, pre-preparation, food handling after cooking, and holding on display in product flow of Nang-Meon and pre-preparation, preparation, and holding on display in product flow of Pi-bim bab. It need for effective quality control of Nang-Meon and Pi-bim bab that training program consist of surveillance, education of the staff, standard operation procedures, forbidding dangerous processes and control of critical points.

• 농업과학연구
• /
• 제17권1호
• /
• pp.18-25
• /
• 1990

The above results indicate that vegetable crop production using vinyl houses continously increases. However, technical support and facility improvement are still insufficent to establish a full production system. In order to improve the cultural environment in facilities for better vegetable culture, several facters should be considered. 1. Facility design and arrangement should be made to improve micro-environments for crop growth and development because direction of facility and covering materials can affect the amount of light trasmitted into vinyl houses. 2. Cultivation of several leading varieties in each crop may not provide stable production and profit especially under undesirable environmental conditions. Thus, it is required to grow not only leading varieties but also other cultivars to decrease the economic losses. 3. Crops should be carefully managed when planted in early season not to experience low temperatures which induce growth retards. Early season cultivation requires proper selection of cultivars which are resistant to low temperture. 4. Active control of micro-environment in facilities should be made via improving the ventilation and irrigation systems.