• Korean Journal of Animal Reproduction
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• v.8 no.1
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• pp.29-35
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• 1984

These experiments were carried out to obtain basic information necessary for in vitro culture of aggregated mouse embryos. Inbred ICR mice were used to obtain embryos. The zona pellucida was removed by placing the embryos in Whittingham's medium containing 0.5% protease for about 5-10minutes at 37$^{\circ}C$. Total 263 pairs of 2-, 4- and 8-cell zona free mouse embryos were subjected to aggregation by physical pressure and cultured in Whittingham's medium under the gas phase of 5% CO2 in air at 37$^{\circ}C$ for 24 to 60 hours. The results obtained in these experiments were summarized as follows: 1. Time needed for fusion of 2-, 4- and 8-cell embryos were 0-3, 0-3 and 0-3 hours, respectively and average time needed for in vitro development of 2-, 4- and 8-cell embryos after aggregation to morula and blastocyst were 42, 30 and 13.5 hours, and 51, 39 and 27 hours, respectively. 2. Of total 263 pairs of naked embryos, 227 were firmly aggregated together and the rats of aggregation in 2-, 4- and 8-cell embryos were 71.8, 88.3 and 97.0%, respectively. 3. The rates of aggregated pairs which obtained from 2-, 4- and 8-cell embryos developed to morula were 96.7, 95.6 and 96.9%, respectively, and embryos developed to blastocysts were 88.5, 89.7 and 90.8%, respectively. 4. Conspicuous differences in size of volume and inner cell masses between single and double blastocysts were observed. Although a single blastocolic cavity was formed in most double blastocysts, several formed two distinct cavities from the very beginning.

• Journal of Fashion Business
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• v.13 no.2
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• pp.30-49
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• 2009

This study was intended to research how such Japonisme affected the western fashion and to consider that the unique Japanese fashion was implemented in the western mode as the traditional aesthetic sense got jointed with the western culture together and was expressed as the modern Japonisme beauty. The beauty of 'Date' was the aesthetic sense created focused on the noble culture of public family. On the contrary, 'Iki', 'Wabi', 'Sabi' and 'Shibusa' is the Japanese lower middle class's universal living aesthetics. 'Kawaii' is influence on an animation, cartoon and character industry as well as the whole world’s fashion style as one of the common aesthetic sense of the world people in the modern time. That is, Japanese fashion designers expressed the human body with such the aesthetic sense by the design of shape, space, fusion, amusement through clothes. It could be concluded that they created an autonomous amusement’s design world over the visual beauty of human body.

• Journal of Embryo Transfer
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• v.14 no.2
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• pp.93-97
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• 1999

The development potential of bovine somatic cells was evaluated using nuclear transfer. A single donor cell derived from fetus of HanWoo(Korean Native Cattle) was selected and deposited into perivitelline space of each enucleated oocyte before electrical fusion and activation. Nuclei of donor cells starved for 7 days (37%) tended to support the development of reconstitute embryo the blastocyst stage better than those of donor cells starved 3, 14 and 30 days. The cleavage rate was significantly lower(P<0.05) in reconstitute embryos derived from large size donor cells(51.2%), than those from small medium size donor cells(76.6 and 73.5, respectively). The developmental rate to blastocyst of reconstructed embryos from medium size donor cells was higher than those from small and medium size donor cells. This study demonstrates that an appropriate culture period for induction into quiescent stage and the size of donor cells effect on the efficiency of nuclear transfer using cultured bovine cells.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.694-701
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• 2014

Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots ($93.33{\pm}4.63%$), roots ($76.67{\pm}7.85%$), and calli ($80.00{\pm}7.43%$) when cultured on Gamborg B5 (B5) medium containing $10{\mu}M$ 6-benzylaminopurine (BA) + $1{\mu}M$ naphthalene acetic acid (NAA), $0.5{\mu}M$ BA + $5{\mu}M$ NAA, and $1{\mu}M$ BA + $10{\mu}M$ NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to $10{\mu}M$. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

• International Journal of Advanced Culture Technology
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• v.3 no.2
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• pp.118-123
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• 2015

IT environment gets more complicated in terms of open platform, network standards, device design and hardware, etc. Smart network and application are in the fields of corporate as well as national competition for future fusion technology. In development environment focused on computers, the ideas of authoring tool have been presented in terms of improved software productivity. In smart environment where subdividing works are consecutively done, current authoring tool should be effectively updated for effective development of programs and easier access to business works. The basic concept of a new conceptual App development tool, Smart App Authoring Tool, which has been designed in this study and enables to apply on-site requirements to smart phones, is to develop Apps on the level using easy-to-learn Word or Excel in a computer. Therefore, this study is intended to design a conceptual Smart App Authoring Tool to optimize the cost and time for developing and maintaining new application services under various smart phone platform environments. Based on the performance of smart app authoring tool herein, every people can develop a smart app program at moderate level. So this paper have designed a conceptual smart app authoring tool. This study presented educational efficiency of the authoring tool by developing business Apps under various business environments and applying them under university and high school environments.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.216-217
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• 2014

Science & Technology(S&T) is the most important thing to attain competitiveness in the 21st century. Our traditional S&T policies have been forcused on hardware infra structure. We should consider software of S&T to enhance effectiveness. S&T Should be had a correct understanding method of the creation for S&T culture. It implies that S&T policy should consider nonmaterial factors which include social, environmental and culture. Under this background, The purpose of this paper is to seek new S&T policy. Therefor this paper deals with how we can prepare for the future S&T policy and classify the change of S&T policy of korea. In addition, Recent construction sector integration and fusion technologies in various areas of research and planning system is being maintained by the system. Past experience of conservative management in this emerging technology-driven technology commercialization in management of technology through the convergence of various technologies and to find synergies to secure the future of construction technology because it is a need.

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.64-69
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• 2017

The rapid growth of algae occurs can induce the algae bloom when nutrients are supplied from anthropogenic sources such as fertilizer, animal waste or sewage in runoff the water currents or upwelling naturally. The algae blooms creates the human health problem in the environment as well as in the water resource managers including hypoxic dead zones and harmful toxins and pose challenges to water treatment systems. The algal blooms in the source water in water treatment systems affects the drinking water taste & odor while clogging or damaging filtration systems and putting a strain on the systems designed to remove algal toxins from the source water. This paper propose the emerging In-Situ self-diagnosable smart algae sensing device with wireless connectivity for smart remote monitoring and control. In this research, we developed the In-Site Algae diagnosable sensing device with wireless sensor network (WSN) connectivity with Optical Biological Sensor and environmental sensor to monitor the water treatment systems. The proposed system emulated in real-time on the water treatment plant and functional evaluation parameters are presented as part of the conceptual proof to the proposed research.

• KSBB Journal
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• v.5 no.1
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• pp.43-47
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• 1990

It was determined optimal Culture conditions and suitable growth regulators for seed germination, growth of callus, and protoplasts derived from cultured and mesophyll cells in Gremastra appendiculata. Induction of fusion between protoplasts of cultured and mesophyll cells was examined. The best conditions of seed germination and growth of callus were achieved on Hyponex medium contained plant growth regulators(2mg/l 2, 4-D, lmg/l Kinetin). Viability and regeneration of cell wall in protoplasts was determined with fluorescence microscope. Also, fused protoplasts were achieved by using PEG solution between protoplasts of cultured and mesophyll cells.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.109-112
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• 2001

Sieve element area and sieve pore formed generally from plasmodesmata. Sieve pore formed by the fusion of several tiny vesicles with plasmodesmata, or those with cell wall after the destruction of special region of newly formed cell wall or those finally with circular arranged form from tissure culture of Streptanthus. The tiny vesicles were produced from dispersed nucleolus or heterochromatin. The sieve area and sieve pore formed from tissue cultured cells were shown round tube form similar to those of natural plants. Sieve area and sieve pore were produced by various methods, and it suggested that the basic materials of the construction of sieve pore originated from the vesicles.

• International Journal of Advanced Culture Technology
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• v.7 no.3
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• pp.143-148
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• 2019

In order to solve the urban problems caused by the increase of the urban population, the construction of smart city applying the latest technology is being carried out all over the world. In particular, we will create a smart city platform that utilizes data generated in the city to collect and store and analyze, thereby enhancing the city's continuous competitiveness and resilience and enhancing the quality of life of citizens. However, existing smart city platforms are not enough to construct a platform for smart city as a platform for solution elements such as IoT platform, big data platform, and AI platform. To complement this, we will reanalyze the existing overseas smart city platform and IoT platform in a comprehensive manner, combine the technical elements applied to it, and apply it to the future Korean smart city platform. This paper aims to investigate the trends of smart city platforms used in domestic and foreign countries and analyze the technology applied to smart city to study smart city platforms that solve various problems of the city such as environment, energy, safety, traffic, environment.