• Title/Summary/Keyword: Culture Dish

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Screening of Humectant to Improve Conservatation of Bacterial Culture Agar Plate (미생물 배양 배지의 보관성을 개선하기 위한 보습성분의 탐색)

  • Hong, Su-Young;Seo, Yu-Mi;Kang, Chang-Youl;Lee, Byung-Woo;Oh, Sung-Kwan;Kwon, Moo-Sik;Lee, Jin-Sung
    • Proceedings of the KAIS Fall Conference
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    • 2009.12a
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    • pp.937-941
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    • 2009
  • 현재 가장 일반적으로 사용되는 미생물 배양 용기인 petri dish를 통해 제조된 미생물 배양 배지의 보관은 냉장과 실온 보관 시 수분 증발에 대한 보호 효과가 극히 낮아 한 달 이상으로 배지 품질을 유지하기 어려운 단점이 있다. 따라서 본 연구는 petri dish를 이용하여 미생물 배양 배지를 제조할 때 glycerol 등 7종의 보습성분을 첨가하여 이에 따른 미생물 배양 배지의 수분 증발 억제 효과와 표준균주별 증식반응을 비교, 분석한 것으로 결과적으로 sodium lactate를 제외한 6종의 보습성분이 배지의 수분 증발을 억제하는 효과가 있으며 이들에 대한 표준균주별 증식반응에서도 양성의 결과를 보여주었다. 앞으로 본 연구의 결과는 즉시 사용 배지(PPM, Pre-Plated Media)의 유통기한 연장 효과 및 품질향상에 유용한 자료로 활용될 수 있을 것으로 기대된다.

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Development of Computer-based Menu Planning Program for Day-Care Centers (탁아기관 급식을 위한 식단작성 전산 프로그램 개발)

  • Kwak, Tong-Kyung;Lee, Hye-Sang;Kim, Sook-Young
    • Journal of the Korean Society of Food Culture
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    • v.7 no.3
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    • pp.245-252
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    • 1992
  • The purpose of the study was to develop computer-based menu planning program for day-care centers maximizing food preference score among children and satisfying such constraints as expense, nutrients, and season. Children's preference about 142 menu items was surveyed among 382 children of day-care centers. A 16-bit personal computer compatible with IBM-PC/AT was used. The data base files were created by dBASE III Plus, and processing programs were created by using FORTRAN language. Children preferred bread or a la carte menu items to cooked rice in main dish category. Deep fat fried or stir-fried menu items were more preferred than kimchi or cooked vegetables in side dish category. Preference scores for menu items were influenced by cooking methods or main ingredients. The contents of the computerized system show that when the program runs, the user should type inputs of cycle, season, and menu pattern, then the computer lists a series of menu satisfying the criteria of constraints. The user can examine and select a set of menu from the menu lists. Menus are generated seasonally. Menu lists are generated weekly and monthly basis with the contents of menu items, preference scores and price. Nutrient reports are also generated on a weekly and monthly basis with the contents of calories, 12 nutrients and price. Recipes for each menu items are also generated.

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Spicy Taste of Korean Traditional Food (한국 전통음식에 사용된 매운 맛)

  • Cho, Woo-Kyoun
    • Journal of the Korean Society of Food Culture
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    • v.26 no.4
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    • pp.374-382
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    • 2011
  • The origin of Korean traditional food's spicy taste dates back to the first telling of the Dangun myth-a story of a tiger and bear who tried to reincarnate themselves in human form by eating garlic and wormwood. For a long time, Koreans have eaten spicy vegetables such as green onion, garlic, ginger, mustard, leeks, corni, cinnamon bark, and Chinese peppers (Zanthoxylum schinfolium) and Zanthoxylum bungeanum. In prehistoric times, spicy vegetables were probably used to eliminate the smell of meat. In the agricultural age, they were used to supplement meals with fresh taste. They were also used as a substitute for salt (salt was very precious and expensive) as well as side dishes for the poor. Spicy vegetables have also been used as a substitute for main dish like medicinal gruel and used to increase the spiciness of soup, and they are usually used as a side dish and with condiments in namul (cooked vegetable dishes), sangchae (salad), ssam (wrapped in greens and garnished with red-pepper paste or other condiments) and Kimchi. In addition, chili pepper was introduced to the Korean Peninsula in the middle of the Joseon Dynasty (mid-15th, 16th century). The soil and climate of the Korean Peninsula are suitable to growing chili pepper, and chili pepper has excellent adaptability and productivity. Accordingly, it is processed to red pepper powder and has become a major part of traditional Korean food along with Chinese pepper. Since the Joseon Dynasty, many kinds of Kimchi made with red pepper powder have been developed, and most Koreans enjoy them these days. The main characteristics of Korean food are spiciness and honest-to-goodness taste.

Defining one Serving Size of Korean Processed Food for Nutrition Labeling (영양성분표시를 위한 우리나라 가공식품의 1인 1회분량 산정 연구)

  • Yang, Il-Sun;Bai, Young-Hee;Hu, Wu-Duk
    • Journal of the Korean Society of Food Culture
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    • v.12 no.5
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    • pp.573-582
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    • 1997
  • The purpose of this study is to establish the one serving size of Korean Processed Food. Defining the one serving size is very important for nutrition labeling and foodservice operation, because the one serving size is used to set up a proper portion by each foodservice operation. The basic data of 200 items were collected through three methods. Searching many cookbooks, exploring the commercial and noncommercial foodservices -6 industrial foodservices, 100 nationwide elementary school foodservice recipes analysis, and 3 hospital foodservice systems as the samples - moreover, experimental cooking and sensory evaluation by trained panels were conducted to assess quantity preference of selected food items. All data were rearranged through food type, that is, main dish, side dish, dessert and health food. One serving sizes of processed foods showed wide variety according to the different menus that include selected food items. Therefore, means and ranges of serving size by three research methods were presented item by item. The results obtained were: 1. The Korean Processed Foods were dried and sugar adding and soused foods, and many of them used the natual processing methods. 2. There were wide varieties in the classification of main dishes, but many of them were cereals, noodles, and sugar products. One serving size of noodles were around $50{\sim}100\;g$, cereals were $20{\sim}40\;g$, which means the one serving size can be differenciated by the food usage. 3. According to the Food classification of side dishes, many of them were as following; natural dried foods, processed fish products, salted or sugar added foods, seasoned foods and sugar products. Moreover the Types of cooking in side dishes were almost culinary vegetables, teas, health foods and condiments, and soused fish products. 4. About desserts, they were almost teas and sugars, and the Types of cooking were teas, health foods and seasonings. 5. We can conclude that almost Korean Processed foods used the drying and soused processing methods for long-time preservation, but it can make the higher content of any special elements, such as sodium or carbohydrates.

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Establishing one Serving Size of Exported Korean Food Items for International Marketing Strategy (수출진흥을 위한 우리나라 전통식품의 1인 1회분량 산정 연구)

  • Yang, Il-Sun;Bai, Young-Hee;Hu, Wu-Duk
    • Journal of the Korean Society of Food Culture
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    • v.12 no.5
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    • pp.509-517
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    • 1997
  • The purpose of this study is to establish the one serving size of Korean Indigeneous Food. Serving size is necessary to make Nutrition Labeling which is required to export Korean food product especially to the United States of America. The basic data of 100 food items were collected through searching traditional and recent cookbooks. 4 industrial foodservices as noncommercial foodservice and 30 traditional ethnic restaurants and 12 gourmet restaurants in hotels as commercial foodservlce were explored to collect the data of actual serving size of each items. Moreover, experimental cooking and sensory evaluation by trained panels were conducted to assess quantity preference of selected food items. All data were rearranged through food type, that is, main dish, side dish, dessert and health food. One serving sizes showed wide variety according to the different menus that include selected food items. Therefore, means and ranges of serving size by four research methods were presented item by item. There were wide differences in intakes of main dishes, for example, noodles were around $50{\sim}100g$, cereals were 20 g, which means the one serving size can be differenciated by the food usage. In intakes of side dishes, average of side dishes were $20{\sim}30g$, but Kimches, the first traditional Korean food, were $30{\sim}50g$, and the other condiments, pepper paste and soy paste were $5{\sim}10g$. About desserts, liquid types were around 200 g, the other sugars were $10{\sim}20g$, the kind of teas were almost $2{\sim}3g$. The health foods-many kinds of that were Ginseng-were averaged 20 g; but dried mushrooms were around 2 g.

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Preparation of Natural Polymer-CaP Composite Films (천연 고분자-칼슘 포스페이트 복합 박막 제조)

  • Kim, Ka-Eun;Mo, Man-Jin;Lee, Woo-Kul
    • Applied Chemistry for Engineering
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    • v.16 no.1
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    • pp.112-116
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    • 2005
  • We investigated the surface modification method for the preparation of organic-inorganic hybrid composite thin film. Gelatin obtained from the decomposition of collagen was allowed to adsorb in a polystyrene tissue culture dish for 2 h to from layers of gelatin. Supersaturated ionic solution of calcium and phosphorus was injected on the gelatin adsorbed layer to form calcium phosphate thin film. During the initial period of incubation, nucleates were formed. With increase of the incubation time, CaP (calcium phosphate) thin film grew on the surface of the culture dish. The gelatin/CaP thin film displayed the highly porous three-dimensional surface structure. Attenuated, total reflectance Fourier transform, infra-red spectroscopy (ATR-FTIR) was used to analyze the chemical properties of CaP film. The analysis demonstrated that the CaP film formed at initial period of treatment appeared to be amorphous. With increase of incubation time, the crystallinity of the film was slightly increased, but the presence of the peaks for the low crystalline CaP confirmed that the CaP thin film prepared in this study was poorly crystallized.

Establishment of Embryonic Stem Cells Derived from Rabbit Embryos (토끼수정란으로부터 배아세포의 분리)

  • 강회성;임경순;최화식;신영수;진동일
    • Korean Journal of Animal Reproduction
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    • v.25 no.3
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    • pp.219-225
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    • 2001
  • To establish rabbit Embryonic Stem (ES) cells, rabbit one-cell embryos were collected and cultured in vitro to blastocysts. Blastocysts were co-cultured with mouse embryonic fibroblasts (MEF), rabbit embryonic fibroblasts (REF) or 570 cells expressing LIF (SNL). Although rabbit ES cells were isolated with low efficiencies, total 8 ES cell lines were kept in vitro with normal colony shape. The MEF was the best feeder for rabbit ES cell isolation in regard to growth rate and undifferentiated morphology. The doubling time of rabbit ES cells in MEF was about 84 hours and the undifferentiated morphology was maintained following passing and freezing processes. These rabbit ES cells were differentiated into embryoid body following the culture in the uncoated dishes, indicating that they were undifferentiated stem cells.

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Preperation and Flavor Characteristics of Seasoning Oil for Chinese Dish (중화요리용 향미유의 제조 및 향미특성)

  • Koo, Bon-Soon
    • Journal of the Korean Society of Food Culture
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    • v.20 no.2
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    • pp.214-220
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    • 2005
  • Seasoning oil (SO) for Chinese dish was manufactured from the combination of Maillard reaction for methionine and xylose with autoclaving method. Volatile compounds were determined by GC, GC-MSD for this SO and 3 kinds of seasoning jajang samples which was obtained from restaurant. From this SO 61 kinds-404.92ppm volatile compounds were separated and identified. And from 3 samples of restaurant, 39 kinds-333.52ppm, 42 kinds-330.01ppm, 42 kinds-393.18ppm obtained respectively. Major volatile components of SO were diallyl disulfide, pentane, diallyl trisulfide, t,t-2,4-decadienal and zinngiberene. Those contents were 40.15ppm, 32.32ppm, 19.57ppm, 15.06ppm and 13.23ppm, respectively. Major volatile components in 3 kinds samples were pentane, propenal, hexanal, t-2-heptanal, 2,4-heptadienal, t,t-2,4-decadienal and unknown components. The volatile components of SO were very similar to 3 samples,

Investigation of Sun-cuisine in Modern Culinary Literature

  • Cho, Woo-Kyoun;Lee, Young-Eun;Lee, Shin-Bi;Cho, Mi-Sook
    • Food Quality and Culture
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    • v.3 no.2
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    • pp.69-73
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    • 2009
  • Sun-cuisine is a traditional Korean side dish. This study examined the methods used to prepare Sun-cuisine in 11 Korean recipe books published over the last 100 years. The main ingredients of Sun-cuisine were typically vegetables, fins, fur, feathers, meat, legumes and mushrooms dipped in wheat flour or mung bean starch powder and stuffed with various minor ingredients known as "so". These dishes are highly seasoned and boiled in meat stock or steamed in a double boiler, after which they were sprinkled with toppings. Various materials are used as the main ingredients. When vegetables were used as the main ingredients, they were sprinkled with salt, sliced and stuffed with beef or mushrooms. Meat stock was then poured on top of the vegetables and they were steamed. A total of 38 food materials were used as the minor ingredients, while 25 materials were used as seasonings and six foods were used as toppings. Pine nuts were widely used as a minor ingredient, seasoning and topping. Sun-cuisine is generally made using various powders such as starch or wheat flour. Sun-cuisine was a kind of royal court food in the past that was served as a side dish. Recently, Sun-cuisine is eaten less often because its cooking process is too delicate and complicated. Therefore, additional studies to enable the modernization of the Sun-cuisine cooking process should be conducted with the goal of revitalizing the beauty and taste of this traditional food.

Consumer's Perceptions of Industrial Foodservice Institutions in Pusan city and Kyeung Nam Provinces -I. Emphasis on the Foodservice Operation- (부산 경남지역 산업체급식소의 급식평가 연구 -I. 급식소 운영특성을 중심으로-)

  • Lee, Myung-Hae;Lyu, Eun-Soon;Kang, Hyeon-Ju
    • Journal of the Korean Society of Food Culture
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    • v.9 no.5
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    • pp.509-524
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    • 1995
  • Consumer's perceptions of the quality of food and food-related servcie were surveyed through questionnaires by 672 adults randomly selected from 20 industrial foodservice in Pusan Kyeung Nam area. Data from consumers were analyzed by using $SPSSPC^+$ program in terms of $x^2-test$, oneway ANOVA, t-test. The results are as follows: 1) Among the food characteristics, the mean rating for temperature of food was 3.18 over 5, for quality of food vs. food price 2.97, for fresshness 2.96, for taste 2.86, and for combination of colors 2.73; 2) In the food-related service, the mean rating was 3.20 for cleanliness of dishes, 3.18 for quality of dishes, 3.01 for varity of food items, 2.95 for courtesy of employees, and 2.57 for availability of favorite food. 3) Self-operated foodservice showed higher mean ratings in nutrition of food, quality of food vs. food price, and availability of favorite food than does the contracted one. 4) The rice (85.0%) and the kimchi (81.4%) were right in amount, but the soup (42.5%) and the side dish (49.0%) were not enough. 5) In seasoning of food, 45.2% of the respondents agreed that the salty taste and the amount of MSG were just right. However, 38.0% and 37.9% of them responded that the salty tast was strong and the amount of MSG was much. 6) The respondents mostly required the improvement of the taste of side dish, nutrition of food, sanitation of food, and variety of menu items.

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