• Title/Summary/Keyword: Culture Differences

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Effects of Culture Duration, Follicle Stimulating Hormone (FSH) Type, and Activin A Concentration on In Vitro Growth of Preantral Follicles and Maturation of Intrafollicular Oocytes

  • Choi, Jung Kyu
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.2
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    • pp.117-122
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    • 2019
  • The objective of this study was to establish an in vitro culture system for ovarian preantral follicles of B6D2F1. First, we optimized the in vitro preantral-follicle culture by culture duration, follicle stimulating hormone (FSH) type, and activin A concentration. Duration of in vitro culture for 9, 11, and 13 days was sufficient for the normal development of preantral follicles to antral follicles. Formation of cumulus cell-oocyte complex (COC) was induced by treatment with human chorionic gonadotropin (hCG; 2.5 IU/mL) and epidermal growth factor (EGF; 5 ng/mL). In addition, metaphase II (MII) oocytes formed during this in vitro culture of preantral follicles. In vitro preantralfollicle culture for 9 days showed higher rates of growth and maturation, thus yielding a greater number of antral follicles, and there were significant differences (p < 0.05) in the number of MII oocytes (that formed from these preantral follicles via differentiation) between the 9-day culture and 11-day or 13-day culture. The follicles cultured for 9 days contained a tightly packed well-defined COC, whereas in follicles cultured for 11 days, the COC was not well defined (spreading was observed in the culture dish); the follicles cultured for 13 days disintegrated and released the oocyte. Second, we compared the growth of the preantral follicles in vitro in the presence of various FSH types. There were no significant differences in the growth and maturation rates and in differentiation into MII oocytes during in vitro culture between preantral follicles supplemented with FSH from Merck and those supplemented with FSH from Sigma. To increase the efficiency of MII oocyte formation, the preantral follicles were cultured at different activin A concentrations (0 to 200 ng/mL). The control follicles, which were not treated with activin A, showed the highest rate of differentiation into antral follicles and into MII oocytes among all the groups (0 to 200 ng/mL). Therefore, activin A (50 to 200 ng/mL) had a negative effect on oocyte maturation. Thus, in this study, we propose an in vitro system of preantral-follicle culture that can serve as a therapeutic strategy for fertility preservation of human oocytes for assisted reproductive medicine, for conservation of endangered species, and for creation of superior breeds.

Analysis on Organization Performance Based on Hospital Culture (기독병원과 일반병원의 조직문화 특성에 따른 조직성과 분석)

  • Kim, Woon-Shin;Nam, Eun-Woo
    • Korea Journal of Hospital Management
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    • v.4 no.2
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    • pp.242-265
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    • 1999
  • Our study attempt is to see organizational performance according to the differences between types of hospital cultures. To determine theoretical relationship between the organizational culture and the performance, we select two hospitals in Pusan which are different in the purposes and shapes of establishment. We surveyed their members on a questionnaire based on the type of the organizational culture of the two institutions, analyzed, and review its organizational effectiveness. For the survey with questionnaires, which had been preliminary studied to raise its validity, question forms were distributed to 528 persons in April, 1999 based on the self-responses and recollected within 48 hours. The recollection rate was high(89.96%) and the quantity of questionnaires used for our final analysis was 430(81.44%). The Cronbach Coefficient Alpha of the questionnaires was 0.742. Regarding statistical techniques for analysis of the written materials, dispersion analysis(ANOVA) was adapted to test the organizational effectiveness of the two hospitals having the different organizational cultures, and Pearson Correlation was applied to determine correlations was among all variables. T-test was performed to test organizational effectiveness based on the differences in the extent of sharing the culture, organizational committment and work satisfaction between the two health institutions. From our analysis, we obtain the following conclusions. First, concerning with organizational culture of the two hospital, one of which is a christian hospital and the other is a private foundation hospital, the former is conservative and human-oriented but the latter focuses on renovation and accomplishment. Second, the private establishment has a relatively higher organizational effectiveness that the religious hospital as a result of analyzing the extent of sharing culture, organizational committment and work satisfaction. Third, it has been found that the correlations between the extent of the sharing culture and the organizational committment, the extent and work satisfaction, and the committment and the satisfaction are respectively positive influencing organizational effectiveness, especially work satisfaction. Fourth, cultural factors by which the christian hospital is affected more positively including human relations among its members, belief, its idea of establishment, tradition, work responsibility, power, and wage. On the other hand, factors such as director's leadership, personnel management, wage, hospital regulations and department managers' management ability have been seen as negative influences in order. And fifth, for the private foundation hospital human relations among its members, wages, work responsibility, director's leadership and department managers' management ability were positive in their sequence while wages, personnel management, hospital regulations, welfare and department managers' management ability were considered as negative influences in order. As these results of this study, the higher extent of sharing organizational culture, the more increasing in both organization committment and work satisfaction, the higher the effectiveness. Although it was somewhat difficult to generalize the results whose subjects were the two hospitals only, it was obvious that organizational culture was an important influential factor of organizational effectiveness. It is questionable that the extent of sharing organizational culture, organizational committment and work satisfaction as variables affecting the effectiveness have their validity, but this study has its significance in that it provided an approaching to evaluate the organizational culture of individual hospitals making allowances for such variables related to the general activities in its hospital. We hope the results of the study could be useful for the managerial strategies of the institutions.

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The organizational culture, psychological empowerment, and organization efficiency in fashion companies (패션기업의 조직문화, 심리적 임파워먼트 및 조직유효성에 관한 연구)

  • Lee, Eun-Jin
    • The Research Journal of the Costume Culture
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    • v.24 no.2
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    • pp.198-217
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    • 2016
  • This study analyzed the effects of organizational culture on psychological empowerment and organization efficiency, and the effects of psychological empowerment on organization efficiency in fashion companies. In addition, this study analyzed the differences between organizational culture, psychological empowerment and organization efficiency across demographic characteristics. The survey was conducted among employees of fashion companies, and 320 responses were used in the data analysis. As a result, the group culture and innovative culture in fashion companies positively influenced the competence and meaning of psychological empowerment. An innovative culture and rational culture positively influenced the self-determination, and the group culture and rational culture positively influenced the impact of psychological empowerment. The group culture, innovative culture, and rational culture of fashion companies positively influenced job satisfaction, and the group culture, innovative culture, and hierarchical culture positively influenced job commitment. The group culture and innovative culture negatively influenced the turnover intention, but the hierarchical culture positively influenced the turnover intention. In addition, the psychological empowerment (competence, self-determination, impact, and meaning) positively influenced job satisfaction, and the competence, self-determination, and impact positively influenced job commitment. Competence negatively influenced the turnover intention, but the impact positively influenced the turnover intention. There was a difference in the group culture and turnover intention by ages, as well as a difference in the hierarchical culture and self-determination by work departments. The results of this study will be helpful for the establishment of the management and business strategies in fashion companies.

Research on the Recognition of the Ritual Ceremony (제례의식의 인지도 조사연구)

  • 김정미;김종군;장성현
    • Journal of the East Asian Society of Dietary Life
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    • v.13 no.3
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    • pp.145-154
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    • 2003
  • This research was performed to identify the characteristics of the Korean rituals and to establish the desirable ritual culture. The recognition of the ritual culture was investigated by the general environmental factors such as gender, age, religion and education background. The rituals were generally recognized as positive. Some significant differences were shown according to religion, gender and residential area. To succeed the tradition of the preparation and the table-setting of ritual foods and to conduct the rituals by the descendants were recognized relatively high by the Buddhists and males living In Gyeongsangbuk-do. The degree of recognition of the method of table-setting of ritual foods was higher if one was married and older, with Buddhism and the oldest daughter-in-law in a family. As above, the culture of Korean rituals showed some differences according to the general environment and regions of the individual. The reason why regional differences were shown high was mainly because tradition and conservatism were maintained according to the unique regional thought and natural environment rather than impacts of the changes of times. That some differences were shown according to general environmental factors suggested that the traditional ritual culture was gradually changing according to the environmental changes in the modem times. Also, the recognition on the ritual culture were identified through investigations by general environmental factors such as gender, age, religion and education background.

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The Relationship of Organizational Culture and Organizational Effectiveness at the General Hospital (종합병원에서 조직문화와 조직유효성과의 관계)

  • Jo, Heui-Sug;Cho, Woo-Hyun;Chun, Ki-Hong;Moon, Ok-Ryun;Lee, Sun-Hee
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.3
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    • pp.374-382
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    • 1999
  • Objectives: Organizational culture has beer important in field of organizational behavior research for the past decade. Although there has been a growing interest in the organizational culture and organizational effectiveness, there is few research in health care field. This study was carried out to investigate the relationship of organizational culture and organizational effectiveness at general hospital. Methods: Data was collected by self-administrated questionares. Organizational cultures were measured by using Likert scale. A general hospital in Kyunggi-Do was selected and survey was conducted to 675 workers. Data was analyzed with computer package, PC-SPSS. Results: There were four types of organizational culture in this hospital consensual culture, developmental culture, hierarchical culture, rational culture. Many workers recognized their culture as rational culture and developmental culture. This finding showed that the hospital had both human related and task related climate. There were some differences in recognition of sub-organizational culture by occupational group, but perceived organizational culture was in accordance with sub-organizational culture in general. Multiple regression analysis and multiple logistic regression analysis were conducted to find the relationship of organizational culture and organizational effectiveness. As a result, developmental culture showed a strong relationship with organizational commitment and job-satisfaction. Conclusions: These results showed that types of organizational culture were significantly related to organizational effectiveness and understanding the existing culture is essential to develope their organizational effectiveness.

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Perception of marriage and marriage preparation - Consumers' age-related behavior differences - (웨딩소비자의 연령에 따른 결혼인식 및 결혼준비행동 차이에 관한 연구)

  • Cho, Seongmi;Yu, Jihun
    • The Research Journal of the Costume Culture
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    • v.27 no.5
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    • pp.461-478
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    • 2019
  • As the concept of "marriage age" gradually disappears, the consumption class of the wedding market includes not only X and Y generations but also Z generations, and each generation has its own characteristics as it is already known, and there are differences in the consumption. So this research analyzed age-related behavior differences in both awareness of and preparation for marriage. Three hundred sixteen unmarried Korean males and females comprised the study population and data was collected from March 5, 2019, to April 3, 2019. The SPSS Statistics 23.0 Package was used for analysis, specifically the functions of frequency, cross tabulation, factor analysis, chi-squared test, Cronbach's alpha, Duncans's new multiple range test (MRT), and analysis of variance (ANOVA). Marriage motivation was analyzed by three factors, and there were significant differences in two types. Marital involvement was analyzed by two factors, and both factors showed significant age-related differences. Concerns relating to marriage preparation were analyzed by four factors, and two varied according to respondent age. Regarding marriage preparation behaviors, the analysis revealed that the marriage preparation method appreciably differed between age groups. Our analysis also found significant age-related differences in "the main media usually used to acquire information" and "the paths preferred for acquiring information in preparing for marriage". We expect that study results will be useful for identifying new research directions, understanding the dimensions of the wedding industry, and developing related marketing strategies.

Differences between Male Nurses and Female Nurses Recognized by Female Nurses (여성간호사에게 인식된 남성간호사와 여성간호사의 상이점)

  • Kim, HyunSu;Yun, HeeJang
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.2
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    • pp.147-155
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    • 2019
  • The purpose of this study is to analyze the difference between female nurse and male nurse in female nurse by applying the phenomenological method of Colaizzi which is one of the qualitative research methodology and to analyze the difference between female nurse and male nurse. What is the difference between a male nurse and a female nurse perceived by a female nurse? This study investigated the differences between female nurses and male nurses with research problems. The participants in this study were 13 female nurses who agreed to participate in the study after explaining and understanding the purpose of the study. The recruitment of the subjects was applied by the snowball method. As a result of this study, five categories were found. The categories were different in clinical tasks, the burden of family economic life, differences in interpersonal relationships, differences in nursing organization, and differences in unknown areas. The results of this study can be used as teaching materials and research data to expand the understanding of difference between male nurse and female nurse.

Perceptional Dimensions and Patterns of Korean Traditional Food and Culture - Comparisons Between Yanbian and Korean Housewives - (한국전통음식과 식생활 문화에 대한 인식차원 및 유형 - 연변 조선족 주부와 한국 주부에 대한 비교적 접근 -)

  • Chung, Young-Sook;Park, Young-Sun
    • Journal of the Korean Society of Food Culture
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    • v.17 no.4
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    • pp.446-455
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    • 2002
  • The purpose of this study was to identify the dimensions and patterns of the perception of Korean traditional food and to find the determinants of the patterns between Korean and Yanbian housewives, taking food culture into account. Data were factor and cluster analyzed, and the results revealed two and three different dimensions and patterns for Yanbian and Korean housewives respectively. Descriptive statistics showed that perceptional patterns are likely to vary depending on cultural background of Korean traditional food. Similarities and differences in perceptional patterns between Yanbian and Korean housewives are discussed, and future implications for food as well as nutrition specialists and food marketers are provided.

Effects of Co-Culture with Oviductal Cells, Time of Transfer into Culture Medium after Insemination on Early Development of In Vitro Fertilized Bovine Oocytes (소 체외수정란의 초기발생에 있어서 수정후 발생배지로 옮기는 시기와 난관상피세포의 영향)

  • 김정익;박춘근;오세훈
    • Korean Journal of Animal Reproduction
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    • v.17 no.2
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    • pp.121-125
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    • 1993
  • Early development of bovine oocytes fertilized in vitro in the medium with caffeine and heparin was examined in different culture systems. When the oocytes were transferred into culture medium 8 h after insemination, 12%(7/60) of penetrated oocytes cleaved to 4-cell stage 24 h after insemination. The proportions of oocytes cleaved to 80to 16-cell stage 48 h after insemination had also a to be higher in oocytes transferred into culture medium 8 h (29%) than 16 h(10%) or 24 h(4%) after insemination. 52% of the 4-cell embryos developed to morula and blastocyst stages when they were co-cultured with oviductal epithelia, whereas only 5% of embryos cultured without the epithelial cells(P<0.001). In another experiment, embryos were co-cultured with ampulla, isthmus or utero-tubal junction of oviducts. There are no significant differences in the proportions of embryos developed to morula and blastocyst stage.

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Conjoint Analysis on the Academia-Industrial Cooperative Research Project Attributes for Culture Technology Research (문화기술 관련 산학협력 연구과제의 속성들에 대한 컨조인트 분석)

  • Jung, Uk
    • Korean Management Science Review
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    • v.29 no.3
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    • pp.13-21
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    • 2012
  • As culture technology is recognized as the important element of national industry, the necessity of the related policy development for enhancement of culture technology is magnified. In this study, we use conjoint analysis to investigate how the researchers from industry and academia think differently on the importance of several attributes in government-supported culture technology research project such as research period, size of research fund, inter-affiliated research requirement and interdisciplinary research requirement, and preference on those attribute levels. Also, we describes how a simulation experiment is designed to simulate participation shares of four alternative hypothetical research project profiles. This study will help establish the more effective policy of the national research program by understanding the preferential differences between industry and academia on the research project attributes.