• Journal of Dairy Science and Biotechnology
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• v.14 no.2
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• pp.157-164
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• 1996

This study aimed increase the quality during ripening of Cheddar cheese made with proteinase-negative mutant of Streptococcus lactis KCTC 1913 selected by curing. The degradation of protein during cheese ripening were investigated by electrophoresis and chromatography. The results were summarized as follow ; 1. The number of lactic acid bacteria decreased with the ripening stage, and that of the control cheese decreased faster than that of the cheese made with mutant. 2. Polyacrylamide gel electrophoretic analysis of cheese caseins revealed no difference between the cheese made with mutant and the control cheese, but differences along with the ripening stage were evident. 3. On Sephadex G-25 column chromatography, the extracts of bitter components from the green cheese and 3 month ripended cheese were fractionated into 3 fractions. With the progress of ripening, bitter peptides were degraded to rather small peptides or free amino acids. 4. Sensory evaluation of the 3 month ripended Cheddar cheese found no significant differences in color but the cheese made with mutant evidenced higher palatability in flavor and better texture than the control cheese. 5. The yields of the cheddar cheese made with mutant was 0.14% higher than that of the control cheese.

• Reproductive and Developmental Biology
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• v.28 no.2
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• pp.83-87
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• 2004

The objective of this study was performed to establish the in vitro culture system of porcine in vitro maturation and in vitro fertilization(IVM/IVF) embryo. These studies was to determine the effects of melatonin, nitric oxide donor(SNP), and the combination effects of SNP and melatonin in porcine IVM/IVF embryos. In routine porcine IVM/IVF procedure, oocytes were cultured for 40∼44h incubation, and the zygotes were cultured for 40∼44h in NCSU 23 medium. Then 2 to 8 cell embryos were removed cumulus cell and were allotted randomly to NCSU 23 containing different concentration of melatonin, SNP and SNP plus melatonin in 5% $O_2$, 5% $CO_2$ and 90% $N_2$ at 38.5$^{\circ}C$. Cell numbers of blastocyst were also counted using double fluorescence stain method. In NCSU 23 medium treated with melatonin 0, 1, 5 and 10 nM, the developmental rate of morula plus blastocysts were 33.3%, 39.1%, 33.3% and 27.9%, respectivly. This result show that the developmental rate of morula and blascytocys treated with 1 nM melatonin was higher than in any other groups(P<0.05). The developmental rates of morula plus blastocysts were 41.9% in 0 uM SNP, 25.6% in 50 uM and 28.4% in 100 uM, respectively. The developmental rate of morula plus blastocysts were decreased treated with SNP in NCSU 23. In combined effects of SNP plus melatonin (0, SNP 50 uM, SNP 50 uM plus melatonin 1 nM, SNP 50 uM plus melatonin 5 nM and SNP 50 uM plus melatonin 10 nM), the developmental rates beyond morula stage of porcine embryos were 31.3%, 34.1%, 39.5%, 29.4% and 39.5%, respectively. The addition of SNP 50 uM plus maltonin 1 nM, developmental rates of blastocyst was higher rate than in any other groups. Cell numbers of blastocyst in NCSU 23 treated with melatonin 0, 1, 5 and 10 nM were 41.0, 42.6, 39.6 and 33.0, respectively. In combined effects of SNP plus melatonin (0, SNP 50 uM, SNP 50 uM plus melatonin 1 nM , SNP 50 uM plus melatonin 5 nM and SNP 50 uM plus melatonin 10 nM), cell numbers of developed blastocyst were 36.3, 34.6, 39.0, 39.9 and 39.0, respectively. These result show that the cell numbers of blastocyst treated with 0, 1 and 5 nM melatonin were higher than in 10 nM group(P<0.05), but cell numbers of blatocyst produced by SNP plus melatonin were not significantly difference in all experimental groups.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.2
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• pp.126-136
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• 2008

Tobacco plants grown in pots by sand culture for 70 days after transplanting were used to evaluate the sensing distance and measurement efficiency of ground-based remote sensors. The leaf distribution of tobacco plant and sensing distance from the sensors to the target leaves were controlled by two removal methods of leaves, top-down and bottom-up removal. In the case of top-down removal, the canopy reflectance was measured by the sensor located at a fixed position having an optimum distance from the detector to the uppermost leaf of tobacco every time that the higher leaves were one at a time. The measurement of bottom-up removal, a the other hand, was conducted in the same manner as that of the top-down removal except that the lower leaves were removed one by one. Canopy reflectance measurements were made with hand held spectral sensors including the active sensors such as $GreenSeeker^{TM}$ red and green, $Crop\;Circle\;ACS-210^{TM}$ red and amber, the passive sensors of $Crop\:Circle^{TM}$, and spectroradiometer $SD2000^{TM}$. The reflectance indices by all sensors were generally affected by the upper canopy condition rather than lower canopy condition of tobacco regardless of sensor type, passive or active. The reflectance measurement by $GreenSeeker^{TM}$ was affected sensitively at measurement distance longer than 120 cm, the upper limit of effective sensing distance, beyond which measurement errors are appreciable. In case of the passive sensors that has no upper limit of effective distance and $Crop\;Circle^{TM}(ACS210)$ that has the upper limit of effective sensing distance specified with 213 cm, longer than that of estimated distance, the measurement efficiency affected by the sensing distance showed no difference. This result suggests that it is necessary to use the sensor specified optimum distance. The result revealed that active sensors are more superior than their passive counterparts in establishing between the relative ratio of reflectance index and the dry weight of tobacco treated by top-down removal, and in the evaluation of biomass. $The\;Crop\;Circle\;ACS-210^{TM}$ red was proved to have the highest efficiency of measurement, followed by $Crop\;Circle^{TM}(ACS210)$ amber and $GreenSeeker^{TM}$ red, $Crop\;Circle^{TM}$ passive, $GreenSeeker^{TM}$ green, and spectroradiometer, in descending order.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.53-58
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• 1988

This study was carried out to determine effect of butachlor [N.-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide] and bialaphos [2-amino-4(hydroxy)(methyl) phosphionyl] butyryl-alanylalanine sodium salt on the germination of tobacco seed, induction and growth of callus from tobacco. Further, fatty acids and ammonia content of tobacco calli were determined. Bialaphos had no effect on tobacco seed germination, but the growth of seedling was markedly affected by an application of 10 ppm bialaphos. However, regardless of varieties tested, tobacco seed germination was completely inhibited by $5{\times}10^{-5}M$ of butachlor. At an application of $5{\times}10^{-5}M$ butachlor, tobacco seeds were to some extent germinated and showed further growth. Hyangcho among varieties tested, showed the most tolerant response to butachlor. In induction of callus from various tobacco varieties and their growth, aromatic type of tobacco varieties exhibited the most tolerance against bialaphos. However, no distinct varietal differences were determined in the treatment of butachlor. The major fatty acids identified in tobacco calli were palmitic, oleic and linoleic acid. No marked difference in terms of fatty acids was observed among tobacco varieties used, but it was observed that there was the higher ratio of quantity in unsaturated fatty acids over saturated one, bialaphos treatment accumulated about 9 times higher ammonia content than that of the untreated control, giving an evidence that bialaphos might inhibit glutamine synthetase activity.

• The Korean Society of Law and Medicine
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• v.19 no.2
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• pp.235-261
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• 2018

The Medical law stipulates regulations about the physician's duty to inform to contribute to patient's self-determination. This law was most recently revised on December 20, 2016, and came into effect on June 21, 2017. There has been much controversy about this, and it has been questioned whether or not it will be effective for physicians to comply with the duty to inform. Therefore, this study investigated perceptions of physicians of whether they observed the duty to inform and their legal judgment about that duty, and analyzed how the revision of the medical law may have affected the legal cognition of physician's duty to inform. This study was conducted through an online questionnaire survey involving 109 physicians over 2 weeks from March 29 to April 12, 2018, and 108 of the collected data were used for analysis. The questionnaire was developed by revising and supplementing the previous research (Lee, 2004). It consisted of 41 items, including 26 items related to the experience of and legal judgment about the duty to inform, 6 items related to awareness of revised medical law, and 9 items on general characteristics. The data were analyzed using SAS 9.4 program and descriptive statistics, Chi-square test, Fisher's exact test and Binary logistic regression were performed. The results are as follows. • Out of eight situations, the median number of situations that did not fulfill the duty to inform was 5 (IQR, 4-6). In addition, 12 respondents (11%) answered that they did not fulfill the duty to inform in all eight cases, while only one (1%) responded that he/she performed explanation obligations in all cases. • The median number of the legal judgment score on the duty to inform was 8 out of 13 (IQR, 7-9), and the scores ranged from a minimum of 4 (4 respondents) to a maximum of 11 (3 respondents). • More than half of the respondents (n=26, 52%) were unaware of the revision of the medical law, 27 (25%) were aware of the fact that the medical law had been revised, 20(18%) had a rough knowledge of the contents of the law, and only 5(5%) said they knew the contents of the law in detail. The level of awareness of the revised medical law was statistically significant difference according to respondents' sex (p<.49), age (p<.0001), career (p<.0001), working type (p<.024), and department (p<.049). • There was no statistically significant relationship between the level of awareness of the revised medical law and the level of legal judgment on the duty to inform. These results suggest that efforts to improve the implementation and cognition of physician's duty to inform are needed, and it is difficult to expect a direct positive effect from the legal regulations per se. Considering the distinct characteristics of medical institutions and hierarchical organizational culture of physicians, it is necessary to develop a credible guideline on the duty to inform within the medical system, and to strengthen the education of physicians about their duty to inform and its purpose.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.5 no.2
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• pp.150-157
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• 2002

Purpose: As the incidence of non-typhoidal salmonella strains resistant to antibiotics has been increased, we attempted to investigate clinical aspects of non-typhoidal salmonella gastroenteritis and antibiotics resistance. Methods: From January 2000 to June 2002, 99 children with positive stool culture of non-typhoidal salmonella were studied about clinical features, the incidence of antibiotics and multi-drug resistance and the difference of incidence of antibiotics resistance according to immune status. Results: There were 66 males and 33 females. The majority of them were under 5 years of age (71%). 25 children were immunocompromised due to chemotherapy, steroid or immunosuppressive treatment. Serogroup D was the most common isolates (65%) followed by B (16%), C (8%) and E (8%). Resistance rate of 30% to ampicillin, 12% to chloramphenicol, 20% to trimethoprim-sulfamethoxazole (TMP-SMX), 11% to cefotaxime and 8% to cefixime were obtained. All isolates were susceptible to ciprofloxacine. Resistance rate to cefotaxime and cefixime in immunocompromised patients was 24% and 14.3% respectively, which were significantly higher compared to that in immunocompetent patients (6.8%, 5.6%, p<0.05). 11 isolates were resistant to three or more antibiotics. The incidence of multi-drug resistant isolates was significantly higher in immunocompromised patients (24%) than that of immunocompetent patients (6.8%). Conclusion: Because of the high prevalence of non-typhoidal salmonella strains resistant to ampicillin, chloramphenicol and TMP-SMX, third-generation cephalosporin might be the treatment of choice in non-typhoidal salmonella gastroenteritis. In particular, antibiotics should be carefully selected in immunocompromised patients because non-typhoidal salmonellas from them showed the higher incidence of antibiotic resistance and multi-drug resistance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.3
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• pp.207-211
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• 1985

This study was conducted to determine the effects of shade (full day light; 100, 80, 60, 40 and 20%) and fertilization levels (high; $N\;30kg-P_2O_5\;35kg-K_2O\;30kg$, moderate; $N\;20kg-P_2O_5\;25kg-K_2O\;20kg$, and low; $N\;10kg-P_2O_5\;15kg-K_2O\;10kg$) of forest pasture on intake palatability of Korean native goat, and the effects of shade under the conditions of 50% shaded forest pasture and full day light pastures on grazing behavior. This experiment was done at the experimental field of Chungnam National University, in Taejon, from 1983 to 1984. The results obtained are summarized as follows: 1. The dry matter intake, R.I.I. and Touch number of Korean native goat were affected by the shade degrees and fertilization levels, generally the increase of the fertilization level and high shading degree increased the NO_3-N$ contents in the plants, but decreased the soluble carbohydrate contents. It means that the decrease of the utilization of the shade culture grasses is due to the decreased intake palatability of forest pastures, particularly the conditions high fertilization level and above 60% of shading level seemed to have a nitrate stress. 2. Grazing time of forest pastures by Korean native goat tended to be spend more time than full day light pastures, also tendency of the major grazing activity tended to be graze continuously without difference of variation of grazing time within a day. Considering of the characteristies os shade, the intensive utilization of forest pastures seemed to be most effective during the high temperature season in August.

• Cross-Cultural Studies
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• v.28
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• pp.115-139
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• 2012

Interval or duration of segments, syllables, words and phrases is an important acoustic feature which influences the naturalness of speech. A number of cross-sectional studies regarding acoustic characteristics of children's speech development found that intervals of segments, syllables, words and phrases tend to change with the growing age. One hypothesis assumed that decreases in intervals would be greater when children were younger and smaller decreases in intervals when older (Thelen,1991), it has been supported by quite a number of researches on the basis of cross-sectional studies (Tingley & Allen,1975; Kent & Forner,1980; Chermak & Schneiderman, 1986), but the other hypothesis predicted that decreases in intervals would be smaller when children were younger and greater decreases in intervals when older (Smith, Kenney & Hussain, 1996). Researchers seem to come up with conflicting postulations and inconsistent results about the change trends concerning intervals of segments, syllables, words and phrases, leaving it as an issue unresolved. Most acoustic investigations of children's speech production have been conducted via cross-sectional designs, which involves studying several groups of children. So far, there are only a few longitudinal studies. This issue needs more longitudinal investigations; moreover, the acoustic measures of the intervals of child speech are hardly available. All former studies focus on word stages excluding the babbling stages especially the canonical babbling stage, but we need to find out when concrete changes of intervals begin to occur and what causes the changes. Therefore, we conducted an acoustic study of interval characteristics of segments and words concerning Canonical Babble ( CB) and early speech in an infant aged from 0;9 to 2;4 acquiring Mandarin Chinese. The current research addresses the following two questions: 1. Whether decreases in interval would be greater when children were younger and smaller when they were older or vice versa? 2. Whether the child speech concerning the acoustic features of interval drifts in the direction of the language they are exposed to? The female infant whose L1 was Southern Mandarin living in Changsha was audio- and video-taped at her home for about one hour almost on a weekly basis during her age range from 0;9 to 2;4 under natural observation by us investigators. The recordings were digitized. Parts of the digitized material were labeled. All the repetitions were excluded. The utterances were extracted from 44 sessions ranging from 30 minutes to one hour. The utterances were divided into segments as well as syllable-sized units. Age stages are 0;9-1;0,1;1-1;5, 1;6-2;0, 2;1-2;4. The subject was a monolingual normal child from parents with a good education. The infant was audio-and video-taped in her home almost every week. The data were digitized, segments and syllables from 44 sessions spanning the transition from babble to speech were transcribed in narrow IPA and coded for analysis. Babble was coded from age 0;9-1;0, and words were coded from 1;0 to 2;4, the data has been checked by two professionally trained persons who majored in phonetics. The present investigation is a longitudinal analysis of some temporal characteristics of the child speech during the age periods of 0;9-1;0, 1;1-1;5, 1;6-2;0, 2;1-2;4. The answer to Research Question 1 is that our results are in agreement with neither of the hypotheses. One hypothesis assumed that decreases in intervals would be greater when children were younger and smaller decreases in intervals when older (Thelen,1991); but the other hypothesis predicted that decreases in intervals would be smaller when children were younger and greater decreases in intervals when older (Smith, Kenney & Hussain, 1996). On the whole, there is a tendency of decrease in segmental and syllabic duration with the growing age, but the changes are not drastic and abrupt. For example, /a/ after /k/ in Table 1 has greater decrease during 1;1-1;5, while /a/ after /p/, /t/ and /w/ has greater decrease during 2;1-2;4. /ka/ has greater decrease during 1;1-1;5, while /ta/ and /na/ has greater decrease during 2;1-2;4.Across the age periods, interval change experiences lots of fluctuation all the time. The answer to Research Question 2 is yes. Babbling stage is a period in which the children's acoustic features of intervals of segments, syllables, words and phrases is shifted in the direction of the language to be learned, babbling and children's speech emergence is greatly influenced by ambient language. The phonetic changes in terms of duration would go on until as late as 10-12 years of age before reaching adult-like levels. Definitely, with the increase of exposure to ambient language, the variation would be less and less until they attain the adult-like competence. Via the analysis of the SPSS 15.0, the decrease of segmental and syllabic intervals across the four age periods proves to be of no significant difference (p>0.05). It means that the change of segmental and syllabic intervals is continuous. It reveals that the process of child speech development is gradual and cumulative.

• FLOWER RESEARCH JOURNAL
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• v.16 no.1
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• pp.36-43
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• 2008

This purpose of this study was to examine the effect of planting density, growing medium and strength of a nutrient solution (National Horticultural Research Institute's nutrient solution: HRI's) on the growth and development of Oriental hybrid lily 'Le Reve' in a box cultivation. The planting density with 14, 18 and 22 bulbs had sprouting one day earlier than other treatments. Planting density of 22 bulbs flowered first, while six bulbs flowered the last, indicating that higher planting densities led earlier flowering. The increasing planting density increased stem length of cut flowers. On the other hand, cut flower quality was improved when the planting density was lower. The incidence of physiological disorders such as blasting was more frequent in planting density of 22, 18, and 14, indicating that higher planting densities caused higher incidences of physiological disorders. All planting densities except 22 bulbs displayed superior results in width, weight, number, and scale weight of the bulbs. Greater planting densities led to inferior bulb enlargement and an increased decomposition rate. pH decreased in all treatments after the bulb enlargement and decreased more as the planting density increased. Contents of P, K, Ca, and Mg increased, while contents of K and Ca decreased, as the planting density increased. The rice hull+coir (1:1, v/v) treatment was better than others, but did not show that much of a difference. Moreover, in bulbs enlargement after cut flower harvest, lily medium and perlite+peat moss treatments showed superior results, and decomposition rate was the greatest in the rice hull+coir (1:1, v/v) treatment. In the HRI's solution strength treatment from the period of flower bud emergence to flower harvest, higher solution strengths gave better cut flower quality in terns of length, weight, and number of flowers. The non-treated control and one third strength of a HRI's solution hastened flowering, indicating that lower strengths led to earlier flowering. According to the results of leaf analysis as affected by solution strength during the flower harvest, absorption rates of N and K were greater when the strength was higher, and Ca and Mg showed the same tendency. On the other hand, the absorption rate of P was the lowest in all treatments.

• The Journal of Korean Society for School & Community Health Education
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• v.4
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• pp.117-139
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• 2003

The purpose of this research lies in presenting logical viability for the measures that curtail access to lewd Internet contents by middle school students amidst the reality in which lewd contents are circulated freely through the Internet, a medium that the middle school students find most easy to access. In order to establish right form of awareness towards sex, this research identified the ways they access the lewd Internet contents, their reaction after the exposure to those contents, their knowledge of sex, their concerns regarding sex and their accessibility to sexual activities in order to conduct a comparative analysis on the relationship between lewd Internet contents and their awareness of sex. First, realities of accessing lewd Internet contents and reactions according to the demographics of middle school students There isa significant difference in the experience of accessing lewd Internet contents in terms of gender. Mostly, male students tend to access the contents more. As for the way they access the lewd Internet contents, both male and female students replied that they access through spam mail of lewd nature. Thus, measures to address this problem are needed urgently. As to when they first accessed the lewd contents, most of the research subjects replied that they accessed either in elementary school period or in the early middle school period. This shows that most of the students got exposed to lewd contents even before they could establish positive, correct awareness of sex. Thus, there is a risk that they may formulate wrong kind of sexual awareness. Accordingly, it is necessary to develop measures through focused sex education. Students are divided into two groups according to the time they spend on the contents averagely: those who spend over one hour and those who spend less than an hour on the lewd contents. If the students spending longer hours are not to be checked and properly guided, it may lead to increasing cases of sexual delinquencies due to their wrongly formed awareness of sex. When the question of existence(non-existence) of guardian was addressed, students with both parents tend to access the lewd Internet contents in a more diverse manner and tend to access more compared to those students from single parent or no-parent families. Accordingly, guardians need to pay attention to how their children are using the Internet. Second, awareness of sex depending on the middle school students' demographics In case of sexual knowledge, middle school students shows relatively high level of knowledge. In particular, female students are found more knowledgeable than male students, and the students in upper years are more knowledgeable as well. As a result, this research recommends that the students in lower years should be guided with more basc and detailed information, while those in upper years need to be taught to form and express their own thoughts and attitudes and to build up independence on this matter. In case of worries about sex, both male and female students don't worry too much about it. However, male students are more concerned about sex than female students in a more diverse ways. As for the differences by academic year, concerns for sex increase, as students get older. Accordingly, sex education that helps establish sound perception of the opposite sex and that focuses on the etiquettes that one must adhere to at the presence of the opposite sex need to be conducted against middle school students. In case of accessibility to sex, male students manifest higher tendency than female students. As for the differences by academic year, those in the first and second years show higher accessibility than those in their third year. In general, younger students tend to be more open-minded toward sex. Accordingly, students in lower academic years need to undergo basic knowledge oriented sex education, whereas those in upper academic years need to undergo discussion centered sex education where they subject to questions regarding their attitude and opinion. Third, relationship between the reactions after the exposure to the lewd Internet contents and their awareness of sex As the frequency of contact with lewd Internet contents increases, awareness of sex increases as well. Thus, the lewd Internet contents contribute to the increase in students' concern for sex as well as increase in accessibility to sex, which eventually become barriers to students' establishment of a healthy perception of sex. Reactions to sex after the exposure to lewd Internet contents and awareness of sex indicate a significant correlation. However, negative correlation is manifested with knowledge of sex. Thus, it is possible to know that the greater access to lewd Internet contents does not necessarily translate into increase in knowledge of sex. However, the study showed there is a correlation between concerns for sex and the level of accessibility to sex. In more detail, the more reactions to the contents they show, the more concerns for sex they have and the more positively they take acceptability to sex. Moreover, it is necessary to develop necessary measures since textbooks today do not include measures needed to address the lewd Internet contents. Given the above findings, it is necessary to continue to complement structural measures in order to prevent easy access of lewd Internet contents by middle school students. Moreover, it is necessary to be considerate of the students so that they themselves can form a healthy Internet culture and grow up within positive framework for the sex education.