• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.564-570
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• 2005

In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%. The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis. For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important in the suspension culture. When the NS0 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic.

• 한국수정란이식학회지
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• 제28권3호
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• pp.273-280
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• 2013

The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.

• 대한응급의학회지
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• 제29권5호
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• pp.474-484
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• 2018

Objective: The time to positivity (TTP) of blood culture reflects bacterial load and has been reported to be associated with outcome in bloodstream infections. This study was performed to evaluate the relationship between the TTP of blood culture and the mortality rates associated with sepsis and septic shock according to the site of infection. Methods: We performed a retrospective cohort study on patients with sepsis and septic shock. The rates of blood culture positivity and mortality as well as the relationship between the TTP and 28-day mortality rate were compared among patients with different sites of infection, such as the lungs, abdomen, urogenital tract, and other sites. Results: A total of 2,668 patients were included, and the overall mortality rate was 21.6%. The rates of blood culture positivity and mortality were different among the different infection sites. There was no relationship between the TTP and mortality rates of total, lung, and urogenital infections. Patients with abdominal infections showed a negative correlation between the TTP and 28-day mortality rate. In patients with abdominal infections, a TTP<20 hours was independently associated with 28-day mortality compared with patients with negative blood culture (hazard ratio, 1.73; 95% confidence interval, 1.16-2.58). However, there was no difference in mortality rates of patients with a $TTP{\geq}20$ hours and a negative blood culture. Conclusion: The shorter TTP in patients with abdominal infections in sepsis and septic shock was associated with a higher 28-day mortality rate.

• 한국항행학회논문지
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• 제28권1호
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• pp.44-50
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• 2024

본 연구는 국내 민간 항공사 조종사 193명을 대상으로 비행 스트레스가 직무 만족도 및 안전문화에 미치는 영향을 분석하였다. 분석 결과, 비행 스트레스는 직무 만족도에 부정적 영향을 미쳤으며, 직무 만족도가 낮은 조종사는 안전문화 인식도 낮게 나타났다. 특히, 항공사와 조종사의 직급에 따라 비행 스트레스가 직무 만족도와 안전문화에 미치는 영향에 차이를 보였다. 이는 항공사와 조종사 직급별로 맞춤형 스트레스 관리 프로그램 개발의 필요성을 시사한다. 연구는 항공 산업의 안전 문화 강화를 위해 조종사의 스트레스 관리와 직무 만족도 향상이 중요함을 강조한다. 또한, 항공사는 조종사의 스트레스를 감소시키고, 직무 만족도를 높여 안전문화를 강화하는 전략을 개발해야 한다. 본 연구 결과는 항공산업에서 조종사들의 스트레스 관리와 안전문화 강화를 위한 방안 모색에 유용한 기초 자료로 활용하기 위함이다.

• 복식문화연구
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• 제24권3호
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• pp.336-348
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• 2016

This study was executed to deduce the factors affecting eco-friendly behaviors and attitudes, to analyse the difference of eco-friendly behaviors and attitudes between countries, and to draw the variables affecting clothing recycling behavior. The sample comprised people from the UK, China, South Korea. The results were as follows. The four factors related to eco-friendly behaviors and the five factors related to eco-friendly attitudes were derived from the results of factor analysis. In the case of eco-friendly behaviors, four eco-friendly behavior factors showed a significant difference all according to gender and country. In the case of eco-friendly attitudes, saving of natural resources for eco-friendly life, recycling for eco-friendly life, and individual preference for eco-friendly life showed a significant according to gender, also recycling for eco-friendly life, individual preference for eco-friendly life, social awareness for eco-friendly life, and company's awareness for eco-friendly life showed a significant difference according to country. All of the variables related to clothing recycling behaviors showed the significant difference according to gender and countries. It was verified that the stronger the behaviors or attitudes for waste recycling and environmental protection, individual preference for eco-friendly life, saving of natural resources, buying eco-friendly products, and separate collection for recycling, the more positive the action to clothing recycling. The results of this study will be helpful to establish a marketing strategy for each country and to deduce a plan to attract clothing recycling form people.

• 복식문화연구
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• 제19권3호
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• pp.650-660
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• 2011

Purpose of this study is to explore: 1) the difference in color sensibility evaluation, color attractiveness, and attitude toward the product, 2) the difference in the effect of color sensibility evaluation on color attractiveness, and 3) the difference in the effect of color sensibility evaluation on attitude toward the product between the identical clothing product presented in on-line and off-line settings. Experimental method was applied with 230 male and female university students using stimuli of an on-line site as well as an off-line window display presenting the same white t-shirts. Color sensibility factors of white t-shirt were hedonic, stimulating, active, and spatial senses. These color sensibility factors of the clothing product affected product color attractiveness as well as attitude toward the product. Although there were not differences in color sensibility evaluation, product attractiveness, and attitude toward the product between items presented in on-line and off-line settings, difference was found in the effect of color sensibility on the color attractiveness and attitude toward the product. The effect of color sensibility on color attractiveness and attitude toward the product was stronger in on-line than in off-line setting. Hedonic sense was the most important factor influencing attractiveness of product color and attitude toward the product. In addition, spatial sense affected attractiveness of product color in on-line setting; and stimulating sense impacted attitude toward the product in off-line setting. Based on the results implications were generated.

• 중소기업융합학회논문지
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• 제6권2호
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• pp.1-9
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• 2016

현대기업경영에서 ERP 시스템은 기업의 전사적인 자원을 효율적으로 배치하고, 활용하는데 있어서 필수적인 도구로 자리매김하고 있다. ERP 시스템의 구현은 단순히 단위정보시스템의 도입이 아니라, BPR을 통한 전사적인 변화와 혁신이 필연적으로 이루어지게 되며, 이를 토대로 시스템 설계와 구축이 이루어지게 된다. 문화적 차이는 경영활동에서 기업 구성원의 태도와 인간관계 뿐 아니라, 조직문화와 기업의 운영원칙의 차이를 만들어낸다. 문화적 차이는 크게는 동양과 서양의 문화권에 대한 차이를 거론할 수 있으며, 작게는 같은 문화권 내의 각 국가마다의 차이를 말할 수 있다. 본 논문은 문화적 차이가 ERP 시스템의 구축과정과 시스템에 미치는 영향을 확인하였다. 이에 대해 ERP 시스템의 구축과정과 의사결정과정, 사용자 Interface에 대한 부분에서 문화적 차이가 미치는 각각의 영향을 분석했으며, 아울러 이에 따른 한국의 문화적 차이에 따른 영향도 분석하였다.

• 복식문화연구
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• 제15권2호
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• pp.326-338
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• 2007

This paper examines the works produced from 2000, the first year of the new millenium, to the late 2006 with a focus on those of Jean Paul Gaultier, a representative designer of deconstruction, in an effort to determine the aesthetic characters of deconstructional fashion. In this context, this paper deals with the theoretical study on the concepts and features of deconstruction as a part of analysis into its fashion using the works of Gaultier as a major source viewed to typically demonstrate the deconstructional characters of fashion. For this purpose, a survey was conducted to 155 works of Gaultier featured from 2001 to 2006 mostly in Vogue, the popular and influencing magazine of the USA(39 pieces in 2001, 30 in 2002, 29 in 2003, 23 in 2004, 12 in 2005 and 22 in 2006, respectively). In particular, 106 fashion pictures were put to analysis as they seemed to represent deconstruction intensively. The deconstructional characters of his works were examined in the categories of difference, inter-meaning, inter-textuality and decomposition on the basis of preceding studies, which resulted in the following outcomes: First, difference was found in 38 works out of 106 images, accounting for 36%, the biggest frequency; Second, inter-meaning accounted for 33% with 35 pieces, most frequently next to difference; Third, inter-textuality followed inter-meaning with 17 images or 15% in terms of frequency; Fourth, decomposition was revealed in 16 images in the same ratio of 15% as inter-textuality.

• International Journal of Advanced Culture Technology
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• 제8권3호
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• pp.307-315
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• 2020

The physique and health status of Chinese college students have received a generous concern in recent years. The physical condition of Chinese college students is not good and this phenomenon is especially obvious among female college students, which has been an disputable fact. This study starts from exercise intervention to study the concrete influence of taijiquan (a kind of traditional Chinese shadow boxing) and five-animal exercises on human body balance ability; the study uses experimental apparatus to measure the specific impact of exercise intervention on human body balance ability; meanwhile, the study also applies data analysis to longitudinally study whether the two studied sports items affect body balance, and whether there is difference in terms of the influence of two sports items on human body balance ability. The conclusions are as follows: firstly, 24-style taijiquan and five-animal exercises can improve ankle muscle strength, and the focus and degree of the improvement are different; secondly, five-animal exercises have a better improvement effect on the flexor and extensor muscles of ankle joint and 24-style taijiquan is in the second place. Moreover, compared with 24-style taijiquan, there is no significant difference in terms of the improvement caused by five-animal exercises; thirdly, 24-style taijiquan has a better effect on the improvement of internal rotation and evertors muscle strength. Compared with 24-style taijiquan, five-animal exercises have no significant difference in terms of the improvement degree.

• 한국식생활문화학회지
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• 제12권2호
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• pp.137-147
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• 1997

The purpose of this study was to determine the influences on physical status and study achievement by the dietary behavior of the youth in Korea. The survey was carried from June to July, 1995 by way of questionnaire to 397 students. The students' study achievement records and physical status were analyzed by the factor of dietary behavior. The results were as follows: 1 The average physical status of male students was lean, but females were standard. 76ft of mothers of the subjects were working in specialty farming. The monthly income of their family was $1000{\sim}1500$ thousand Won and students' pocket money was $20{\sim}40$ thousand Won per month. 2. The aspects of meal behavior included the general idea that the meal was thought to relieve hunger, and preference of taste was spiciness The most important factor of the meals for the students was taste. Their bad meal habits were quick eating and irregularity of meals. 3. There was a significant difference in the comparison of the study achieving records with the frequency of supper However, there was no one meaningful difference between study achievement records and the attendance rate to school, health status, and living conditions. However, there was a significant difference between their study records and eating with family. The students, who had the breakfast and/or supper with family, remarked better study records than other groups. 4. Also, there was a significant difference of study records with physical status. The overweight or obese students had a lower degree of study score (p<0.1). Adolescence is considered a period of setting up dietary behavior, so it is necessary to educate good nutrition and dietary habits for students to keep in healthy physical condition and with high study achievements.