• 한국가금학회지
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• 제22권2호
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• pp.77-84
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• 1995

In order to investigate the effect of feeding live yeast culture on the performance of laying hens, a feeding trial was conducted with 96 20-wk-old Hy4ine brown layers during their laying period of 60 wk. The live yeast culture used was a product from Saccharomyces cerevisiae that was cultured on the corn-based substrate followed by careful drying of whole material not to lose the viability of yeast. Three levels of yeast culture as 0.5, 1.0, and 2.0% for three treatments and 0% for the control were included in the experimental diets. The feeding trial was carried out for 60 wk from August 26, 1992 to October 26, 1993. To evaluate the performance of layers during cold or hot periods as affected by the yeast culture feeding, data from the 12-wk winter period and 12-wk summer period were separated and analyzed accordingly. During 60 wk of laying period hen-day egg production was slightly but significantly(P<.05) improved by feeding the yeast culture. The average egg weight and daily egg weight(g /day) were also increased by the yeast culture. Feeding the yeast culture did not increase feed intake but feed efficiency was improved significantly (P<.05). No significant difference was detected in egg or eggshell qualities between control and yeast culture-treated groups. Feed intake and egg weight were not affected by the yeast culture feeding under both cold and hot period, but egg production and feed efficiency during hot summer improved significantly by its feeding. This result indicates that the effectiveness of the yeast culture feeding is greater during summer than winter for laying hens.

• 한국수정란이식학회지
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• 제27권3호
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• pp.189-192
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• 2012

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, ${\alpha}$-MEM or KnockOut$^{TM}$-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, ${\alpha}$-MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and ${\alpha}$-MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, ${\alpha}$-MEM tended to be more suitable after passage 6. Although ${\alpha}$-MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.

• Clinical and Experimental Reproductive Medicine
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• 제47권4호
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• pp.284-292
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• 2020

Objective: This study investigated whether adding outer-well medium to inhibit osmotic changes in culture media in a dry-type incubator improved the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) cycles. Methods: In culture dishes, the osmotic changes in media (20 µL)-covered oil with or without outer-well medium (humid or dry culture conditions, respectively) were compared after 3 days of incubation in a dry-type incubator. One-step (Origio) and G1/G2 (Vitrolife) media were used. Results: The osmotic changes in the dry culture condition (308 mOsm) were higher than in the humid culture conditions (285-290 mOsm) after 3 days of incubation. In day 3 IVF-ET cycles, although the pregnancy rate did not significantly differ between the dry (46.2%) and humid culture (51.0%) groups, the rates of abortion and ongoing pregnancy were significantly better in the humid culture group (1.5% and 49.5%, respectively) than in the dry culture group (8.3% and 37.8%, respectively, p<0.05). In day 5 IVF-ET cycles, the abortion rate was significantly lower in the humid culture group (2.2%) than in the dry culture group (25.0%, p<0.01), but no statistically significant difference was observed in the rates of clinical and ongoing pregnancy between the dry (50.0% and 25.0%, respectively) and humid culture groups (59.5% and 57.3%, respectively) because of the small number of cycles. Conclusion: Hyperosmotic changes in media occurred in a dry-type incubator by evaporation, although the medium was covered with oil. These osmotic changes were efficiently inhibited by supplementation of outer-well medium, which resulted in improved pregnancy outcomes.

• 생명과학회지
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• 제19권12호
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• pp.1764-1768
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• 2009

본 연구는 소의 과립막세포 배양상층액에서 스테로이드 호르몬의 농도와 생쥐 배의 체외 발달에 미치는 효과를 검토하기 위하여 수행되었다. 소의 성숙난포(직경 6~15 mm)와 미성숙 난포(직경 2~5 mm)로부터 과립막세포를 각각 분리하여 15% FCS가 첨가된 Ham's F-10 배양약에서 16일간 배양하였다. 과립막세포들은 쉽게 단층배엽을 형성 하였으며, 배양과정 중 비슷한 성장패턴을 나타내었다. 또한 성숙 과립막세포와 미성숙 과립막세포 간에 형태적인 차이가 없었다. 과립막세포 배양 중 progesterone과 estradiol 생산이 활발하게 이루어졌으며, progesterone은 배양후기에, estradiol은 배양초기에 분비량이 높았다. 성숙 과립막세포와 미성숙 과립막세포에서 내분비 양상은 비슷하였다. 생쥐배가 상실배, 배반포 및 부화배반포 단계로 체외발달된 비율은 Ham'F-10배양액(86.7%, 41.7%, 13.3%)에 비하여 성숙 과립막세포 배양상층액(92.7%, 78.1%, 34.5%)과 미성숙 과립막세포 배양상층액(96.4%, 78.5%, 26.8%)에서 유의적으로 높았다(p<0.05). 그러나 배의 발달에 대하여 성숙 과립막세포배양상층액과 미성숙 과립막세포 배양상층액 간에는 차이가 없었다.

• 한국식생활문화학회지
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• 제31권2호
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• pp.131-140
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• 2016

This study analyzed the Food Neophobia Scale (FNS) and Korean food perception status of Southeast Asian workers living in South Korea in order to build up basic data to develop a desirable diet program. From our study, we found that FNS was higher in the order of Cambodian, Myanmar, Vietnamese, and Thai workers. Influential demographic factors for FNS were cooking possibility and place of residence. The mean scores of Cambodian workers ($3.46{\pm}0.63$) regarding "positive perception of cooking method, taste & color" about Korean food were the highest among the four countries, followed by Vietnam ($3.38{\pm}0.68$), Myanmar ($3.26{\pm}0.50$), Thailand ($3.09{\pm}0.64$)workers(p<0.01). The mean scores of Myanmar workers regarding perception of "difference in cooking method, smell & texture" and "difference in taste" were the highest among the four countries. FNS had a negative correlation with the factor "positive perception of cooking method, taste & color" regarding Korean food and a positive correlation with the factors "difference in cooking method, smell & texture".

• 한국식생활문화학회지
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• 제31권2호
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• pp.188-193
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• 2016

The purposes of this study were to prolong the storage period and maintain quality of kimchi made from spring napa cabbage, which is used less frequently than winter napa cabbage. The results show that the firmness of kimchi from early July, the latest harvest period, was significantly higher than that of kimchi from mid-June. However, as maturation proceeded, no significant difference was observed following the harvest period when kimchi was stored for 8 weeks. Regarding pH and acidity, which are highly related to maturation of kimchi, pH generally decreased from the initial storage period while acidity increased. In the sensory evaluation, appearance was best in kimchi manufactured in late June according to manufacturing and maturation periods (p<0.05). After 4 and 8 weeks of storage, kimchi manufactured in early July had the highest scores with no significant difference. Texture and overall acceptability also showed no significant difference in each processing period.

• 한국식생활문화학회지
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• 제18권5호
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• pp.407-417
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• 2003

This study was performed to investigate nutritional intake, the dietary behaviors and plasma lipid profile between smokers and non-smokers of college male students in the Gyeonggi Area. Dietary behaviors and attitude toward smoking were investigated by questionnaires. The result obtained were as follows: There was no significant difference in anthropometric measurements between smokers and nonsmokers. There was no significant difference in dietary behaviors. However, smokers tended to eat more snacks and desserts than nonsmokers. In plasma lipid levels, smokers had higher levels of plasma triglyceride, LDL-cholesterol, VLDL-cholesterol and total cholesterol than that of non-smokers(p<0.05). HDL-cholesterol level of smokers was a lower than that of non-smokers although the difference was not significant. AI(Atherogenic Index), Total /HDL cholesterol ratio and LDL/HDL cholesterol ratio of smokers were significantly higher than that of non-smokers(p<0.01). BMI and SBP showed positive correlation with triglyceride(p<0.01) and VLDL-cholesterol(p<0.01). DBP showed positive correlation with triglyceride(p<0.001) and VLDL-cholesterol(p<0.01).

• 복식문화연구
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• 제15권6호
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• pp.917-928
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• 2007

The purposes of this study were to investigate the hairdo attitude and hairdo involvement importances and differences depending on age. For the experimental work, questionnaires for research are prepared and asked to people who are women living in Seoul and Kyoung-Ki Do. They are collected from September to October 2006. 406 questionnaires are used in the analysis. The collected data were 'analyzed by using SPSS 12.0 software with factor analysis, Cronbach's alpha, paired T-test, and T-test. The results of this study were as follows; To analyze differences in the hairdo attitude and the hair involvement depending on different age groups, two groups were identified: one group of women aged $20{\sim}35$, and the other group of those aged $45{\sim}60$. 1. When the hairdo attitude factors were prioritized, orientations toward 'constancy' and 'consciousness of others' were found to be most critical, followed by those toward 'leader's fashion conformity' and 'distinct individuality'. When the hairdo involvement factors were prioritized, 'interests and pleasure in hairdo' were found to be most important, followed by 'symbolic representation', 'risk awareness', 'coordination of hairdo', and 'fashionableness'. 2. When difference in the hairdo attitude was analyzed, a significant difference was shown in orientations toward 'leader's fashion conformity' and 'consciousness of others'. 3. As for the hairdo involvement of the groups, a significant difference was found only in 'coordination of hairdo', signalling that the younger women put more focus on the coordination.

• 한국식생활문화학회지
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• 제30권5호
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• pp.587-595
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• 2015

The purpose of this article was to research the difference in additional marketing satisfaction by lifestyle of Korean restaurant customers (focus on foreign students at university in Gangwon province). In this research, factor analysis, cluster analysis, and ANOVA were carried out. Lifestyle was divided into seven factors and six clusters. Additional marketing satisfaction was divided into three factors. Additional marketing satisfaction showed a significant difference by lifestyle of foreign students. First, 'employee' had a highest point at C3 'convenience internet searching pursuit' but lowest point at C4 'brand preference pursuit' (p<0.001). 'Service process' had a highest point at C1 'safety health pursuit' but lowest point at C5 'effort pursuit' (p<0.001). 'Tangible clue' had a highest point at C3 'convenience internet searching pursuit' but lowest point at C5 'effort pursuit' (p<0.001). Additional marketing can reduce customer uncertainty, ambiguities, and wavering about eating at restaurants by intangibility of service. As a result, food-service corporations need to manage additional marketing as well as increase satisfaction and loyalty of customers.

• 경영과정보연구
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• 제19권
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• pp.17-48
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• 2006

While the existing research took approach method that uniformly divided the cultural difference between specific countries into individualism - collectivism in the dimension of comparative culture, this research defines the cultural sense of value of domestic university students and company employees in accordance with horizontalism-verticalism pattern within it to corroboratively define the diverse effects of advertisements of standardization and localization of global enterprises. Researching the difference of cultural sense of value owing to this generation of Korean university students and company employees, Korean university students showed prominent propensity of vertical-individualism value and company employees, propensity of horizontal-collectivism value. Researching comparatively the effect of advertisements for standardization and localization of global enterprises in connection with Korean university students and company employees, it turned out Korean university students prominently prefer localization advertisement in the TV advertisement of global enterprises and company employees, standardization advertisement. Especially, the difference between standardization advertisement and localization advertisement of university students and company employees was very remarkable in trademark attitude and advertisement information rather than advertisement attitude. With respect to model appearing in the advertisement, it turned out university students prefer localization advertisement where domestic models appear and company employees prefer standardization advertisement where foreign models appear.