• Title/Summary/Keyword: Cultural mycelia

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Occurrence of Gray Mold Rot of Perilla Caused by Botrytis cinerea (Botrytis cinerea에 의한 들깨 잿빛곰팡이병의 발생)

  • 문병주;노성환;손영준;강형석;이재필;김병섭;정대수
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.467-472
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    • 1998
  • Gray mold rot of perilla was epidemic at Kangdong, Pusan and Miryang, Kyungnam in 1997 and 1998. The incidence of this disease ranged form 21.3 to 68.1% at Kangdong area. Leaf necrosis initially appeared on the edge of the infected leaves, and it was developed to the center of the leaves forming typical V-shaped brown necrotic lesions. Under high moisture condition, abundant mycelia of the pathogen was formed on the surface of the lesions. Infected stems became slender and were completely blighted up to the top of the plant. Two isolates, LVF12 and SD7, were isolated from diseased lesions showing typical symptoms, and the pathogenicity was tested using mycelial disks and conidial suspension inoculation. The developed symptoms were same as the naturally produced ones. These two pathogenic fungi were identified as Botrytis cinerea based on the morphological characteristics using a microscope and a scanning electron microscope, and cultural characteristics. This is the first report of gray mold or perilla in Korea.

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Occurrence of Fusarium Wilt on Lisianthus (Eustoma grandiflorum) Caused by Fusarium oxysporum f. sp. eustomae (Fusarium oxysporum f. sp. eustomae에 의한 꽃도라지 시들음병(가칭) 발생)

  • 함영일
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.188-190
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    • 1998
  • Fusarium wilt of lisianthus occurred severely throughout all cultivation areas, especially in alpoine areas during summer season and the disease incidence was 5 to 30 percent in Korea. The major symptoms of the disease were wilt with chlorosis and water deficiency, stunted plants and scorched leaves. Severe symptoms appeared just after high temperature period around late August in Daekwallyong area. Whit and pale red colored mycelia were developed on stems of infected plants near the soil surface. The causal organism of Fusarium wilt of lisianthus was isolated and identified as Fusarium oxysporum f. sp. eustomae on the basis of pathogenic and cultural characteristics. The causal organism was reisolated from all infected and inoculated stems of plants. This is the first report referring to F. oxysporum f. sp. eustomae of lisianthus in Korea.

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Cladophialophora lanosa sp. nov., a New Species Isolated from Soil

  • Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • Mycobiology
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    • v.47 no.2
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    • pp.173-179
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    • 2019
  • Cladophialophora is characterized by simple hyphomycetes with brown hyphae that give rise to branched chains of pale brown conidia and shows affinities with the Herpotrichiellaceae. A fungal strain belonging to the genus Cladophialophora was isolated from soil in Daegu, Korea. This strain produces numerous greenish to dark black lanose aerial mycelia with hair like structures. It is morphological similar to C. chaetospira, C. inabaensis, and C. multiseptata; however, the conidiophores and conidia sizes of the newly isolated strain (KNU16-032) are clearly different from them. The novelty of the strain was also confirmed based on phylogenetic analysis using the data sets of the internal transcribed spacer region of and the partial sequence of 28S ribosomal DNA region along with the cultural characteristics. Because morphological observations and phylogenetic analysis indicated that the strain is distinct from previously known Cladophialophora species, we propose this species as a new species Cladophialophora lanosa sp. nov., and provide the detailed descriptions in this study.

Cultural characteristics survey of Clavicorona pyxidata (WANG GWAN) strains collected from Korea (좀나무싸리버섯(Clavicorona pyxidata) '왕관' 수집균주의 배양적 특성조사)

  • Chang, H.Y;Park, S.A.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.12 no.1
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    • pp.11-19
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    • 2010
  • This study was carried out to investigate the characteristics survey of Clavicorona pyxidata strains. C. pyxidata are selected 3 kinds of strains collected in GWANG DEOCK Mt., DO BONG Mt., and BUK HAN Mt., Mycelial growth of C. pyxidata collected from GWANG DEOCK and BUK HAN Mt. is 64mm, 60mm for 18days respectively. DO BONG Mt strain is 85mm for 9 days. Transparent crystal body of C. pyxidata mycelia were appeared by microscopic examination of 100 magnification. Optimal temperature of mycelial growth in C. pyxidata strains are 25~30℃ respectively.

Effect of Cultural Media and Conditions on the Mycelial Growth of Pholiota adiposa (배지와 배양조건이 검은비늘버섯의 균사생장에 미치는 영향)

  • Lee, Byeong-Seok;Seo, Geon-Sik;Lee, Jong-Soo
    • Journal of Practical Agriculture & Fisheries Research
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    • v.7 no.1
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    • pp.74-80
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    • 2005
  • To elucidate the optimal conditions of mycelial growth of Pholiota adiposa, the effect of a kinds of media, incubation temperature and shaking culture were investigated. Mushroom complete medium was selected as superior media for mycelial growth in this fungus. The optimal temperature for mycelial growth of this fungus shows at 25℃. Shaking culture could be obtain the mycelia in two or three times than the stationary culture.

First Report of Waitea Ring Patch caused by Waitea circinata on Zoysiagrass (Zoysiagrass에 Waitea circinata에 의한 Waitea Ring Patch 발생)

  • Kim, Kyung-Duck;Hong, Sung-Chul;Jang, Kong-Man;Han, Muho;Pyee, Jae-Ho;Park, Dae-Sup
    • Weed & Turfgrass Science
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    • v.3 no.4
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    • pp.378-381
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    • 2014
  • A new pathogen was isolated from zoysiagrass-planted park of Jeju island in 2014. Symptoms appeared a type of irregular patches occurring brownish leaf blight, followed by stem and crown rot. The symptom was very similar to that of large patch caused by Rhizoctonia solani, a well-known devastating zoysiagrass disease. The isolate showed thin orange-colored mycelia and screlotia were formed on the medium based on cultural characteristics. The causal agent of the disease was finally identified as Waitea circinata by analysis of ribosomal DNA. On the inoculation test, Waitea circinatae showed strong pathogenicity to the zoysiagrass. The mycelia were obviously observed in the inoculated tissues. This is the first report of Waitea ring patch caused by Waitea circinata on zoysiagrass.

Morphological and cultural characteristics of a novel Phellinus linteus KACC93057P (신규 목질진흙버섯 KACC93057P의 배양적, 형태적 특성)

  • Min, Gyeong-Jin;Kwak, A-Min;Seok, Sun-Ja;Kang, Hee-Wan
    • Journal of Mushroom
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    • v.14 no.3
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    • pp.75-80
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    • 2016
  • Morphological and culture characteristics of the novel Phellinus linteus variant KACC93057P collected in Korea were characterized in this study. The surface of the was angular, sessile, tough-woody, concentrically zonate, and dark brown in color. Basidiocarppores were circular, with 5-7 pores per mm. The hyphal system was dimitic, and basidiospores were ellipsoid or oval, $4.5-6{\times}4-5$, exhibiting characteristics typical of P. linteus. The optimum temperature for mycelial growth was $25-30^{\circ}C$, and optimal pH for growth was 5-7. The mycelial growth rate of P. linteus KACC93057P was faster than that of other P. baumii isolates. On growth medium, KACC93057P formed aerial mycelia with density higher than that of other isolates. The internal transcribed spacer (ITS)-ribosomal DNA sequences were closely related to the sequences P. linteus complex.

Cultural characteristics of Cordyceps militaris strain 'Yedang 3' on various media and nutritional conditions

  • Lee, Byung-Joo;Lee, Mi-Ae;Kim, Yong-Gyun;Lee, Kwang-Won;Choi, Young-Sang;Lee, Byung-Eui;Song, Ho-Yeon
    • Journal of Mushroom
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    • v.11 no.3
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    • pp.124-130
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    • 2013
  • The effects of environmental and nutritional requirement such as temperature, pH, different media, carbon, nitrogen, and carbon-to-nitrogen ratio on the mycelial growth of Cordyceps militaris strain 'Yedang 3' were studied. The optimum temperature and pH for the growth of mycelium were $20-25^{\circ}C$ and pH 6-7, respectively. Out of ten media tested, mushroom complete media (MCM) was the best medium for fast mycelial growth, and Sabouraud's dextrose agar yeast extract (SDAY), malt extract yeast extract agar (YMA) also were favored. The color and shape of colonies varied in different media. The best carbon sources for mycelial growth were fructose, mannitol, and sucrose, whereas the best nitrogen sources were tryptone and peptone. However, mycelia grew slowly in inorganic nitrogen compounds such as $NH_4Cl$, $(NH_4)_2SO_4$, $NH_4NO_3$, and $NaNO_3$. The optimum C:N ratio observed on the culture media was 30-40 range. These results provided basic information on cultural characteristics of vegetative growth and might be useful for spawn production in Cordyceps militaris.

Biocontrol of root diseases of fruit trees with fungal viruses

  • Matsumoto, Naoyuki;Nakamura, Hitoshi;Ikeda, Kenichi;Arakawa, Masao;Uetake, Yukari
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.19-20
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    • 2003
  • Helicobasidium mompa Tanaka and Resellinia necatrix Prillieux cause violet root rot and white root rot of various crops, respectively. Intensive cultural practices, such as the use of dwarf stock, glasshouse cultivation, etc., predispose plants to the diseases. The diseases can be controlled only by biennial drench of 50100L of chemicals for each tree. Biocontrol with soil microorganisms proved ineffective under field conditions. Long-term control may be hampered by the perennial growth of hosts and by the difficulty in the establishment of antagonists in soil. Crop rotation or soil amendment is not applicable, either. Fungal viruses with dsRNA genome (Buck 1986) are promising against root diseases of fruit trees since they exist within the cytoplasm of fungal hyphae and need no effort to help them persist in the field. The viruses are considered to spread though the network of fungal mycelia in the soil once they enter the fungal cytoplasm. Here, we present preliminary results from a project to control the root diseases of fruit trees with dsRNA.(중략)

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Verticillium Wilt of Potato Caused by Verticillium albo-atrum in Daegwallyong Area in Korea

  • Kim, Jong-Tae;Ryu, Kyoung-Yul;Kim, Jeom-Soon;Hahm, Young-Il;Yu, Seung-Hun
    • The Plant Pathology Journal
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    • v.19 no.3
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    • pp.184-187
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    • 2003
  • Verticillium wilt was first observed in 2001 on potatoes (Solanum tuberosum) cv. Superior at Daegwallyong area, one of the major seed potato producing areas in Korea. The wilted potato plants showed typical symptoms including gradual yellowing and interveinal necrosis. There was discoloration in the vascular tissues of the infected stems which turned light brown. Fungal isolates from discolored vascular tissues were whitish to creamy with folding on potato dextrose agar medium, where they used to produce resting dark mycelia but no micro-sclerotia. Conidiophores were septate with side branches, swelled at the base, and arranged in a whorl. Conidia were 2.5-11.2$\times$2.0-4.5 $\mu\textrm{m}$ um in size and were borne in small clusters at the tips of phialides. Optimal temperature range for mycelial growth was $25-30^{\circ}C$. Based on these cultural and morphological characteristics, the fungus was identified as Verticillium albo-atrum Reink & Berth. Pathogenicity tests by root dipping method revealed that the fungus caused the same symptoms as observed in naturally infected potato plants. This is the first report of Verticillium wilt on potato caused by Verticillium albo-atrum in Korea.