• Journal of Microbiology
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• v.44 no.2
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• pp.155-161
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• 2006

Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63 % (137 of 217) in the standard PCR method to about 34 % (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: ${\alpha},\;{\beta},\;{\gamma}-$ Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivation-based method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.

• Journal of the Korean Association of Geographic Information Studies
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• v.21 no.4
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• pp.108-118
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• 2018

Recently, agricultural sector apply ICT technology such as Smart Farm to pursue innovation in the changing situation that is emerging as the fourth industrial revolution. However, this innovation requires techniques for forecasting and analyzing in various data bases and spatial information provides such infrastructure data. In this study, the cultivation area of Chinese cabbage, radish, garlic, onion, and red pepper were calculated and analyzed by year. The purpose of this analysis is to cope with sudden changes in vegetable crops and changes in cultivated area caused by weather changes to supply and demand of major vegetables and price instability. As a result of this study, spatial information based on time series information of vegetable complex will be used as efficient agricultural environment observation data, as well as interpretation of various spatial ranges such as the estimation of cultivation area using remote sensing.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.48-48
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• 2022

In recent, a demand for sweetpotato cultivation technology to expand the cultivated area of field crops in paddy fields is increasing. This research was carried out to establish suitable varieties and cultivation techniques for mass production of sweetpotato for processing raw materials. For the selection of varieties suitable for cultivation in rice fields for each processing purpose, 12 varieties in 2018 (8 varieties for starch, 4 as dried, chips and beverages), and 6 varieties in 2019 (4 varieties for starch including 'Daeyumi'; chips, semi-dried 'Pungwonmi'; beverage and coloring 'Shinjami') were used. Sweetpotato stems were planted in mid-May and harvested after 120 days to investigate the yield. Results revealed that the yield of sweetpotato (2019) for starch production, varied with variety as 'Gogeonmi' 3,926 > 'Jinhongmi' 3,428 > 'Daeyumi' 2,873 > 'Singeonmi' 2,752 kg/10a. The starch content was 20.2% in 'Daeyumi', 18.2 in 'Gogeonmi', 21.2 in 'Singeonmi', and 20.6% in 'Jinghongmi'. The total amount of starch was higher in 'Daeyumi' (730 kg/10a) and 'Gogeonmi' (731 kg/lOa). The yield of chips and edible varieties 'Pungwonmi' was 4,688 kg/10a. The yield of 'Shinjami' of purple variety such as beverages and powder was 3,139 kg/10a. Aaa result of evaluation sweetpotato yield by waterlogging treatments on different growing stages in paddy fields, the yields of 'Daeyumi' and 'Jinhongmi' varieties treated with waterlogging at the storage root formation stage decreased by 11.8% and 11.7%, respectively, compared to the control. In the case of waterlogging treatment at storage root swelling stage, Both varieties showed the lowest yield reduction at 7.0% and 4.8%, respectively. Based on these results, stable production and substitution effect of processing raw materials can be expected by cultivating sweetpotato varieties suitable for paddy cultivation.

• Korean Journal of Remote Sensing
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• v.36 no.5_1
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• pp.823-833
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• 2020

In order to stably produce crops, there is an increasing demand for effective crop monitoring techniques in domestic agricultural areas. In this manuscript, a cultivation area extraction method by using deep learning model is developed, and then, applied to satellite imagery. Training dataset for crop cultivation areas were generated using RapidEye satellite images that include blue, green, red, red-edge, and NIR bands useful for vegetation and environmental analysis, and using this, we tried to estimate the crop cultivation area of onion and garlic by deep learning model. In order to training the model, atmospheric-corrected RapidEye satellite images were used, and then, a deep learning model using FC-DenseNet, which is one of the representative deep learning models for semantic segmentation, was created. The final crop cultivation area was determined as object-based data through combination with cadastral maps. As a result of the experiment, it was confirmed that the FC-DenseNet model learned using atmospheric-corrected training data can effectively detect crop cultivation areas.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.795-806
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• 2016

This study was conducted to improve the continuous techniques for international competitiveness of ginseng industry to Korea-China FTA negotiation and conclusion, and provide the basic information for ginseng industry development of Korea. It was carried out the visiting of the northeastern three provinces (Jilin, Liaoling and Heilongjang) in China for 3-year from 2014 to 2016 and observed the farmers' fields of ginseng cultivation with soil environmental status. The types of ginseng cultivation could be observed in small scales of 0.5~3.0 ha, in middle scales of 4.0~10.0 ha and in large scales of 30~700 ha with the kinds of imhasam, Chinese ginseng, Korean ginseng and western ginseng. Also ginseng was cultivated in newly reclaimed land of forest in two types of direct seeding and transplanting of ginseng seedlings. The field beds of ginseng growing were covered with vinyl films in arch design of 100~130 cm height and vinyl was painted in spraying with blue, green and yellow colours for shading. It was investigated in status of the physico-chemical properties of soils. The physical information on the field soils were silt loam, loam and sandy loam in soil textures, and some plain in low slope, some alluvial fan or local valley in forest of land topography. Soil pH ranged within 5.0~5.2, soil EC was $0.93{\sim}3.78dS\;m^{-1}$, organic matter was $37{\sim}35g\;kg^{-1}$, nitrate nitrogen $63{\sim}490mg\;kg^{-1}$, available $P_2O_5$ $55{\sim}163mg\;kg^{-1}$, and in exchangeable cations, K was 0.30~0.98, Ca was 6.5~14.0, Mg was $1.1{\sim}5.3cmol_c\;kg^{-1}$ in ranges. Farmers used the fertilizer for ginseng cultivation in 10~11 t of compost, $200{\sim}400kg\;ha^{-1}$ of complex fertilizer and $750kg\;ha^{-1}$ of oil cakes. The northeastern three provinces of China can use the newly lands with large areas of ginseng cultivation in soil sickness by continuous cropping. and the soil basic fertility is batter than that of Korean in standard guide of ginseng cultivation soil.

• The Korean Journal of Community Living Science
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• v.19 no.4
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• pp.659-672
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• 2008

There is a need to develop diverse cultivation species with reference to the favorites and farming experiences of allotment garden participants, in addition to the crops being cultivated in the gardens. In this study, therefore, a survey on the status of cultivation and the demand for herb crops for the garden participants was conducted. Current trend of the programmes related to allotment garden for city dwellers, executed by various relevant organizations was analyzed. A questionnaire was accomplished by adult participants experiencing farming in allotments. Likewise, interview with farm proprietors was conducted. The results showed that the number of crop species cultivated in allotments was about ten species. The purchase of seeds and nursery plants was mainly dependent on the farmland proprietors. The selection of the crops was made by both wife and husband. The status of herb utilization indicated that 5.7% of participants were cultivating or had cultivated herb plants. Sixty-two percent of participants were not cultivating herb plants because they did not know how. The purposes of using herbs were for medicine and beauty (22.0%), for spices (22.0%) and for enjoyment (29.0%). Thirty-four percent (34.0%) of participants were interested or would like to use the herbs in the future for medicine and beauty, 39.0% for spices and 31.7% for enjoyment. It is expected that the demand for herbs would increase. Also, 78.3% of participants had the intension or had taken into consideration to cultivate the herb plants, if the farmland proprietors would provide the plants. And, they had interest in planting, management and post harvest utilization and if a reference material would be provided as well. An approach to plant new crops might be possible to those who have more than four years of farming experience and possess relatively high level of cultivation techniques. In conclusion, the favorable crop cultivation strategy corresponding to the participants' abilities; and rental process were essential for the sustainable operation of allotment gardens and expanded number of participants. Moreover, there is a need to pay attention to the city dwellers who have a lot of farming experiences in allotment gardens to maintain agriculture and rural community. This is so, because they are interested in agriculture and rural community and most of them replied that they had intentions to move to rural areas in the future.

• Journal of Korea Spatial Information System Society
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• v.10 no.3
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• pp.93-106
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• 2008

For agriculture is very highly dependent on climate and weather condistions, global warming seems to have a great impact on it, including its productivity, cultivation condition, product quality, and optimum cultivation location. In this study, we adopted geographical information system (GIS) in order to investigate the changes of Korea's cultivation area which are caused by global warming, especially with the examples of such tropical and sub-tropical fruits as lemon, fig, kiwi, orange, pomegranate, and mandarin. In terms of GIS techniques, we utilized the interpolate function for temperature changes, surface analysis function for slope, and raster calculator. Currently, these fruits's cultivation areas are in Jeju island and southern part of Korea. But these areas will be expanded according as our GIS model assumes $3^{\circ}C$ and $4.5^{\circ}$ increases of average and lowest temperature by the global warming in Korea. Optimum cultivation areas of these six fruits have two patterns; one is expansion and the other is belt shape shift. From the results of the study, we call for an urgent need of Korea government's policy and farmers' reasonable responses about global warming, which will be able to give more opportunities and better foods to Korea society in general.

• Journal of Mushroom
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• v.21 no.4
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• pp.266-269
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• 2023

This study aimed to address the increasing demand for technologies preventing mushroom fly damage. By monitoring the annual occurrence patterns of pests over several years and accumulating data, we conducted an analysis to evaluate the efficacy of preventive measures applied before the surge in mushroom fly infestation, typically observed in May. For preemptive control, physical measures involved installing air curtains at cultivation facility entrances and placing sticky traps and insect traps around entry points to block external entry and reduce internal insect density. Additionally, we applied an organic agricultural material, Dalmatian chrysanthemum extract, weekly alongside chemical control measures. To assess the reduction in mushroom fly populations, yellow sticky traps (15×25 cm) were placed at three locations within the mushroom cultivation facility, and the occurrence patterns before and after implementing preventive measures were compared. Compared to conventional practices, the application of preventive techniques resulted in a significant reduction, with a 60% decrease from 15 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in May and a 40% decrease from 10 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in June. While achieving over 50% efficacy during the peak mushroom fly season with preventive measures, we identified complementary actions such as blocking external sources (gaps in cultivation facility doors) and maintaining cleanliness around cultivation facilities (proper disposal of spent substrate) for further improvement. Comprehensive analysis and safety studies, including correlation analysis with contaminants and pathogens, are recommended to ensure the widespread adoption of mushroom fly preventive techniques for safe and stable mushroom production in the agricultural sector.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.983-991
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• 2012

These days, agricultural products cultivated in facilities occupy the highest percentage of agricultural output price. Specifically cucumbers have been one of the crops that farmers prefer to growing, because their prices were high. However, cucumber crop is sensitive to the soil and environments and it requires the exact crop management. In order to establish cultivation techniques for cucumbers, the current situation of cucumber cultivation was surveyed from ten cucumber farmlands; five farmlands of cucumber cultivation in forcing and five of semi-forcing practicing systems, respectably. The soil conditions were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy in soil texture family. Soil was managed with deep plowing combined with application of basal fertilizers such as compost, rice straw, oil cake, wood chip and chemical fertilizer. The whole soil was prepared in uniformly with rotary. Three major nutrients ($N-P_2O_5-K_2O$)of basal application were 815-464-529 kg $ha^{-1}$ in forcing and 197-135-151 kg $ha^{-1}$ in semi-forcing cultivation. Top dressing of fertilizer was supplied in fertigation system of macro and micro elements in 2~3 day interval with water irrigation. The average yields of cucumbers were $168t\;ha^{-1}$ with 381,000 thousand won $ha^{-1}$ in average gross profit (AGP) in forcing cultivation and $115t\;ha^{-1}$ with 177,000 thousand won $ha^{-1}$ in AGP in semi-forcing cultivation. Cucumber production during the winter season was considered to increase the gross profit because cucumber price tends to stay in high level during this time. The accumulation of soil chemicals like EC, available $P_2O_5$ and exchangeable cations could be controlled by rice straw application. The rice straw application increased soil temperature during the winter season, in exchange of soil air, and in extension of plant roots. In addition, the rice straw application somewhat affected decrease of salts accumulation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.130-131
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• 2005