• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.648-655
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• 2015

Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.281-287
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• 2005

The endoinulinase gene (inu, 2.733 kb, EC 3.2.1.7) from Paenibacillus polymyxa was subcloned into an Escherichia coli-yeast shuttle vector with GALl promoter for the expression in Saccharomyces cerevisiae. The constructed plasmid, pYGENIU27 (8.6 kb) was introduced into S. cerevisiae SEY2102 cell and then the yeast transformant was selected on the synthetic defined media lacking uracil and on the inulin-containing media. The recombinant endoinulinase was predominantly localized in the periplasmic space of the yeast cell. The total activity of the endoinulinase reached 1.81 unit/ml by cultivation of yeast transformant on YPDG medium. The optimized conditions determined for the inulooligosaccharides (IOSs) production from inulin were as follows; pH, 8.0; reaction temperature, $45^{\circ}C$; inulin source, Jerusalem artichoke. Enzyme activity was stably maintained up to the pH of 10.0. Under the optimized condition and with endoinulinase of 36 unit/g-inulin, IOSs started to be produced after 10 min of enzymatic reaction. By the reaction with inulin, IOSs consisting of inulobiose (F2), inulotriose (F3), and inulotetraose (F4) were produced and F3 was the major product. Consequently, these data would be used as a fundamental parameters for the production of functional sweetener IOSs from inulin by recombinant yeast endoinulinase.

• Korean Journal of Weed Science
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• v.14 no.4
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• pp.239-244
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• 1994

As a fundamental study to control Alopeuclus aequalis Sobol. var. amurensis(Kom.) Ohwi., on dominant weed in barley and dry seeded rice cultivation on paddy fields, several factors which would have closer relations to its germination and emergence, were examined. The results are as follows. The dormancy of seeds were broken at dry-heat treatment of $30^{\circ}C$. The germination rate of the seeds was high in order of 15>10>20> $5^{\circ}C$ and was very low at more than $25^{\circ}C$. The emergence of A. aequalis was influenced little for the light, but had a tendency to be good on the dark condition. The seeds dried on room temperature germinated few but them treated on $30^{\circ}C$ for 24 hours were germinated over 80%. The A. aequalis occured few in the saline soil of salt concentration of more than 0.25% and the germination rate of seeds was very low on the over 0.5% solution of NaCl. On the solution of pH 6.0~12.0, the germination was not effected for pH but was very few on pH 2.0. At the paddy-lowland which have cultivated the soybean for three years A. aequalis emerged a few.

• Journal of the Korean association of regional geographers
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• v.11 no.1
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• pp.29-39
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• 2005

Silk Road is the term involving all traffic roads connecting the East to the West, and ancient Korean culture has became globalized through Silk Road. In this paper, natural environment of Silk Road is considered as fundamental data in order to understand the Western culture introduced through Silk Road. Silk Road region is classified into Loess Plateau district; arid basin district; and high mountains district; Hesi Corridor(河西回廊) is the transition zone that the southeastern part of $W{\breve{u}}w{\bar{e}}i$(武威) is Loess Plateau district; other northwestern part is arid basin district Loess Plateau district, composed of homogeneous fines, has experienced dramatic soil loss by bringing under cultivation. At recent times, reforestation and construction of check-dam have been in operation to cope with this problems, Arid basin district; although at midlatitude, is desert climate because it is far from the ocean and is surrounded by high mountains. Oasis of Hesi Corridor is continuous linearly, but that of arid basin district is sporadic. In $H{\bar{a}}mi$ and $T{\breve{u}}l{\breve{u}}f{\bar{a}}n$(吐魯番) fruit-growing using karez flourishes, And glacier of $Ti{\bar{a}}nshian$(天山) Mts. and $Q{\bar{i}}lian$(祁連) Mts. is very important to arid basin district because of supplying water resource. In the tundra zone surrounding alpine glacier, are distributed widely solifluction lobes and earth hummocks.

• Water for future
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• v.9 no.1
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• pp.38-42
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• 1976

Because of differences in thoughts and ideology, our country, Korea has been deprived of national unity for some thirty years of time and tide. To achieve peaceful unification, the cultivation of national strength is of paramount importance. This national strength is also essential if Korea is to take rightful place in the international societies and to have the confidence of these societies. However, national strength can never be achieved in a short time. The fundamental elements in economic development that are directly conducive to the cultivation of national strength can be said to lie in -a stable political system, -exertion of powerful leadership, -cultivation of a spirit of diligence, self-help and cooperation, -modernization of human brain power, and -establishment of a scientific and well planned economic policy and strong enforcement of this policy. Our country, Korea, has attained brilliant economic development in the past 15 years under the strong leadership of president Park Chung Hee. However, there are still many problems to be solved. A few of them are: -housing and home problems, -increasing demand for employment, -increasing demand for staple food and -the need to improve international balance of payment. Solution of the above mentioned problems requires step by step scientific development of each sector and region of our contry. As a spearhead project in regional development, the Saemaul Campaign or new village movement can be cited. The campaign is now spreading throughout the country like a grass fire. However, such campaigns need considerable encouragement and support and the means for the desired development must be provided if the regional and sectoral development program is to sucdceed. The construction of large multipurpose dams in major river basin plays significant role in all aspects of national, regional and sectoral development. It ensures that the water resource, for which there is no substitute, is retained and utilized for irrigation of agricultural areas, production of power for industry, provision of water for domestic and industrial uses and control of river water. Water is the very essence of life and we must conserve and utilize what we have for the betterment of our peoples and their heir. The regional and social impact of construction of a large dam is enormous. It is intended to, and does, dras tically improve the "without-project" socio-economic conditions. A good example of this is the Soyanggang multipurpose dam. This project will significantly contribute to our national strength by utilizing the stored water for the benefit of human life and relief of flood and drought damages. Annual average precipitation in Korea is 1160mm, a comparatively abundant amount. The catchment areas of the Han River, Keum River, and Youngsan River are $62,755\textrm{km}^2$, accounting for 64% of the national total. Approximately 62% of the national population inhabits in this area, and 67% of the national gross product comes from the area. The annual population growth rate of the country is currently estimated at 1.7%, and every year the population growth in urban area increases at a rising rate. The population of Seoul, Pusan, and Taegu, the three major cities in Korea, is equal to one third of our national total. According to the census conducted on October 1, 1975, the population in the urban areas has increased by 384,000, whereas that in rural areas has decreased by 59,000,000 in the past five years. The composition of population between urban and rural areas varied from 41%~59% in 1959 to 48%~52% in 1975. To mitigate this treand towards concentration of population in urban areas, employment opportunities must be provided in regional and rural areas. However, heavy and chemical industries, which mitigate production and employment problems at the same time, must have abundant water and energy. Also increase in staple food production cannot be attained without water. At this point in time, when water demand is rapidly growing, it is essential for the country to provide as much a reservoir capacity as possible to capture the monsoon rainfall, which concentarated in the rainy seaon from June to Septesmber, and conserve the water for year round use. The floods, which at one time we called "the devil" have now become a source of immense benefit to Korea. Let me explain the topographic condition in Korea. In northern and eastern areas we have high mountains and rugged country. Our rivers originate in these mountains and flow in a general southerly or westerly direction throught ancient plains. These plains were formed by progressive deposition of sediments from the mountains and provide our country with large areas of fertile land, emminently suited to settlement and irrigated agricultural development. It is, therefore, quite natural that these areas should become the polar point for our regional development program. Hower, we are fortunate in that we have an additional area or areas, which can be used for agricultural production and settlement of our peoples, particularly those peoples who may be displaced by the formation of our reservoirs. I am speaking of the tidelands along the western and southern coasts. The other day the Ministry of Agriculture and Fishery informed the public of a tideland reclamation of which 400,000 hectares will be used for growing rice as part of our national food self-sufficiency programme. Now, again, we arrive at the need for water, as without it we cannot realize this ambitious programme. And again we need those dams to provide it. As I mentioned before, dams not only provide us with essential water for agriculture, domestic and industrial use, but provide us with electrical energy, as it is generally extremely economical to use the water being release for the former purposes to drive turbines and generators. At the present time we have 13 hydro-electric power plants with an installed capacity of 711,000 kilowatts equal to 16% of our national total. There are about 110 potential dams ites in the country, which could yield about 2,300,000 kilowatts of hydro-electric power. There are about 54 sites suitable for pumped storage which could produce a further 38,600,000 kilowatts of power. All available if we carefully develop our water resources. To summarize, water resource development is essential to the regional development program and the welfare of our people, it must proceed hand-in-hand with other aspects of regional development such as land impovement, high way extension, development of our forests, erosion control, and develop ment of heavy and chemical industries. Through the successful implementation of such an integrated regional development program, we can look forward to a period of national strength, and due recognition of our country by the worlds societies.

• Journal of Bio-Environment Control
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• v.1 no.1
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• pp.1-13
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• 1992

This study was attemped to provide some fundamental data for the safety structural design of biological production facility. Wind speed and snow depth according to recurrence intervals for design load estimation were calculated by frequency analysis using the weather data of 60 stations in Korea. The following results were obtained : 1. Type-I extremal distribution was selected for the probability density function of yearly maximum wind speed and snow depth and result of Chi-square goodness of fit showed highly significance at most regions. 2. Design frequency factors for given number of samples and recurrence intervals were calculated, and also design wind speed and snow depth as shown in Table 5-Table 6 and Fig.3-Fig.4 were derived. 3. About 46.4% of the winds having maximum wind speed at every station was analyzed to be same direction, and the consideration of this fact may improve the structural safety. 4. Considering wind speed and snow depth, protected cultivation is very difficult in Ullungdo and the Youngdong districts, and strong structural design is needed in the Chungnam and Junbuk west seaside against snow depth and the west-south seaside against wind speed in Korea.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.32-42
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• 1995

Cultivation using rain shelter is very popular in summer because rain shelter has a simple structure using less materials than any other regular greenhouse. Although it has a main advantage of easy construction in terms of labour, time and cost, it has some disadvantage of poor ventilation and rain fall inflow. Therefore, the rain shelters being able to overcome the problems, to some extent, are necessary to propagate for practical purpose. Three characteristic types of rain shelter were analyzed using measured and simulated environment data. Type 1 was a conventional type with an arched roof, and Type 2 and Type 3 were improved ones which were designed to have three arched roofs and three sawtooth like roofs with the openings for ventilation, respectively. The distribution of inside temperature measured was relatively uniform in Type 2 and 3 by the natural ventilation through the openings of the roof compared to Type 1 which had no openings. The relative light transmittance measured in Type 2 and 3 showed lower than that in Type 1, which suppressed the rise of inside temperature, For more accurate comparison, the differences between inside and outside temperatures to various wind speeds were calculated by the model. The difference in Type 1 was the greatest at lower wind speed below 1 ㎧, that is, the highest in inside temperature, but decreased rapidly as wind speed increased above 1 ㎧. Measured temperatures generally showed the same trends as calculated ones by the model. As a whole, the improved rain shelters(Type 2 and 3) showed better performance than the conventional one in ventilation as well as inside temperature.

• Journal of Wetlands Research
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• v.25 no.4
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• pp.237-247
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• 2023

Paddy fields not only provide a variety of ecosystem services but also serve as crucial habitats for biodiversity conservation. Recently, their ecological value and significance have been increasingly emphasized. Therefore, this study aimed to investigate the characteristics of soil seed banks and analyze their correlation with soil environmental factors in Conventional Paddy field (CP) and Organic Paddy field (OP) with different farming practices. Our results revealed that the vegetation in CP was simple, resulting in low plant diversity in the soil seed banks. On the other hand, the vegetation in OP was relatively diverse, leading to higher plant diversity in the soil seed banks. Additionally, distinct differences in soil environmental characteristics were observed between OP (K, Ca, Mg, Na, Fe, Mn, B↓) and CP (K, Ca, Mg, Na, Fe, Mn, B↑). These results suggest that variations in agricultural practices for rice cultivation have influenced the structure and diversity of vegetation and soil seed banks. Furthermore, these agricultural practices have had both direct and indirect effects on soil environmental factors. Our findings can serve as fundamental data for evaluating biodiversity conservation in agricultural ecosystems, ecosystem restoration, and ecological value assessment.

• (The)Study of the Eastern Classic
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• no.67
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• pp.203-236
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• 2017

The objective of this study is to suggest the transmission direction and tasks of Ssireum, and the conclusions are like below. First, Ssireum has been designated as a national intangible cultural heritage(No.131) in December 2016. Second, in the current status of preservation and spread of Ssireum, there is no system related to the transmission of Ssireum such as cultivation of professional manpower, and facilities, programs, and administration/finance for transmitting Ssireum. Third, based on the "Intangible Cultural Heritage Preservation & Promotion Act", the transmission direction of Ssireum was suggested. (1)The designation of a college to transmit Ssireum would be needed. (2)The Ssireum curriculum should be established. (3) It would be necessary to secure trainees for transmitting Ssireum. (4)The production of teaching materials for transmitting Ssireum would be needed. (5)It would be needed to secure training institutes designated in each region and also teaching personnels for transmitting Ssireum. (6)The research direction for the correct transmission of Ssireum should be set up. Fourth, based on the "Ssireum Promotion Act", the transmission direction of Ssireum was suggested. (1)It is urgent to complement provisions for transmitting Ssireum. (2)For the transmission of Ssireum, instead of the transmission direction of Ssireum as a national intangible cultural heritage in the standardized perspective, it would be necessary to seek for the joint transmission direction between South & North Korea for research & documentation of Ssireum that could be systematized/shared between South & North Korea, development/spread of contents for discovering the original form of Ssireum, and the establishment of a base of transmission system for the preservation/promotion of Ssireum through the academic/institutional exchanges regarding Ssireum between South and North Korea. Moreover, the overall and fundamental transmission measures for the education, transmission, research, record, and informatization of Ssireum, and the cultivation of professional manpower should be established. Fifth, the contents of institutional tasks for Ssireum are like following. (1)The institutional complementation of the "Ssireum Promotion Act" should be done for the domestic/foreign promotion of Ssireum. (2)For the integration of Ssireum organizations, the administrative system should be unified. (3)The standard technical system manual for Ssireum should be produced. (4)The 'Ssireum Transmission Center' should be built for the preservation and systematic transmission of Ssireum. (5)The selection of a college for transmitting Ssireum and the establishment of a system to cultivate Ssireum successors should be done. (6)It would be necessary to establish database(DB) for the use of Ssireum techniques essential for the transmission of Ssireum.

• Economic and Environmental Geology
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• v.6 no.1
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• pp.29-64
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• 1973

A land resources survey in the semi-arid area, Mula in S. E. Spain, of $400km^2$ is compiled. The basic aim of the project is to investigate the intrinsic qualities of the land resources of the area by means of applying an integrated method of natural resources survey mainly concerning with analysis and synthesis of land complexes, each of them represents an area or a group of areas with similar patterns of landforms, soils and vegetation, based on a geomorphological approach. The area is characterized by a linear arrangement of relief pattern with an asymmetric homoclinal repetition of slope attitudes elongating WSW-ENE, dipping steeply on the NW sides and gently on the SE sides, which have been resulted from the post-Alpine folding of the Triassic to Cretaceous limestone, the Eocene limestone, the Oligocene sandstone and the lower Miocene limestone and marl, and the post-lower Miocene faulting, tilting and subsequent differential erosion of the Miocene sedimentary formations. An integrated body of information in geology, landforms, soils and vegetation, which are significantry interrelated as an environmental complex, has been obtained. Using this data, 26 land complexes developing on the various situations of landforms, such as folded mountain ranges, tilted tablelands, bevelled cuestas, degraded hill-lands associating with enormous foots lopes, undulating terrains and terraced or flat plains, have been differentiated, mapped and described. The soils of the area are mostly light colored calcic lithosols which have been derived dominantly from the marly parent materials and developed into remarkable slope catenas in some places depending on the relief conditions. The land uses of the area are mainly characterized by the perennially irrigated cultivation of citrus orchards along the terraced alluvial deposits fringing the Segura and Mula River, and the dry-land cereal cultivation on gentler slopes. Pioneer dry-land cultivations within the shrubs on steeper slopes are restricted to the unchannelled tributary drainage floors. The availability of water is a fundamental controlling factor for existence of native and cultivated vegetation as a whole, and a number of active processes including sheet wash and gully erosion, especially on the scarp slopes, are the other important factors to be considered in conservation and management of the land in the area.