• 한국유기농업학회지
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• 제17권2호
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• pp.253-264
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• 2009

상추의 가을재배에서 공기 교반 장치로 실내 온도와 습도를 조절해 주면 잿빛곰팡이병(B. cinerea)과 무름병(E. carotovora)이 감소되었으며, 수량도 30% 이상 증수되었다. 배추의 진딧물 밀도는 천적인 무당벌레(H. axyridis)와 콜레마니진디벌(A. colemani)을 방사하며 현저히 감소되었다. 난황유와 BT제를 혼합하여 살포하면 배추의 무름병(E. carotovora), 노균병(P. brassicae), 흰가루병(E. polygoni)의 발생과 배추좀나방(P. xylostella)과 파밤나방(S. exigua)의 발생 밀도가 현저하게 감소하였다. 파의 노균병(P. destructor) 발생은 Neem 추출물을 처리하여 무처리 대비 60% 이상 낮출 수 있었다. 파좀나방의 충해는 잎굴파리고치벌에 의해 23% 감소되었다.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.739-748
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• 2001

A self-organizing fuzzy logic controller using a genetic algorithm is described, which controlled the glucose concentration for the enhancement of flavonoid production in a fed-batch cultivation of Scutellaria baicalensis G. plant cells. The substrate feeding strategy in a fed-batch culture was to increase the flavonoid production by using the proposed kinetic model. For the two-stage culture, the substrate feeding strategy consisted of a first period with 28 g/I of glucose to promote cell growth, followed by a second period with 5 g/I of glucose to promote flavonoid production. A simple fuzzy logic controller and the self-organizing fuzzy logic controller using a genetic algorithm was constructed to control the glucose concentration in a fed-batch culture. The designed fuzzy logic controllers were applied to maintain the glucose concentration at given set-points of the two-stage culture in fed-batch cultivation. The experimental results showed that the self-organizing fuzzy logic controller improved the controller\`s performance, compared with that of the simple fuzzy logic controller. The specific production yield and productivity of flavonoids in the two-stage culture were higher than those in the batch culture.

• 한국식품영양학회지
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• 제24권2호
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• pp.226-232
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• 2011

In this study, for development of functional sprouts, we have investigated the change of antioxidant activity and quantity of vitamin C, germanium and selenium according to the addition of vitamin C, germanium oxide and selenium oxide on the during cultivation of various sprouts. We were cultivated the sprouts using water solution added vitamin C, germanium oxide and selenium oxide on the system of cultivation instrument. Analysis of quantity of vitamin C on control group and treatment group were conducted by HPLC. Quantity of germanium and selenium were analyzed by ICP-MS(Inductively Coupled Plasma-Mass Spectrometer), and antioxidant activities were checked by DPPH method. As a result, quantity of vitamin C, germanium and selenium on the treatment groups have increased tendency compared to the control group. And antioxidant activity of treatment groups showed increasing tendency compared to the control group.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.967-974
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• 2012

Cultivation of Spirulina platensis using ammonium salts or wastewater containing ammonium as alternative nitrogen sources is considered as a commercial way to reduce the production cost. In this research, by analyzing the relationship between biomass production and ammonium-N consumption in the fed-batch culture of Spirulina platensis using ammonium bicarbonate as a nitrogen nutrient source, an online adaptive control strategy based on optical density (OD) measurements for controlling ammonium feeding was presented. The ammonium concentration was successfully controlled between the cell growth inhibitory and limiting concentrations using this OD-based feedback feeding method. As a result, the maximum biomass concentration (2.98 g/l), productivity (0.237 g/l d), nitrogen-to-cell conversion factor (7.32 gX/gN), and contents of protein (64.1%) and chlorophyll (13.4mg/g) obtained by using the OD-based feedback feeding method were higher than those using the constant and variable feeding methods. The OD-based feedback feeding method could be recognized as an applicable way to control ammonium feeding and a benefit for Spirulina platensis cultivations.

• The Plant Pathology Journal
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• 제38권5호
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• pp.472-481
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• 2022

Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.

• 생물환경조절학회지
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• 제16권1호
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• pp.67-71
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• 2007

파슬리의 재배시기별 MA 저장성을 비교하였는데, 먼저 수확 당시 파슬리의 품질을 보면 엽록소 함량은 여름재배, 비타민 C 함량은 가을재배에서 그리고 경도는 겨울재배에서 가장 높게 나타났다. 각각 가을, 겨울 여름에 재배한 파슬리를 0.04mm세라믹 필름으로 포장하여 0$0^{\circ}C$에서 저장한 결과 겨울재배 처리구가 84일간 저장이 가능하였고 다음으로 가을재배가 77일, 여름재배는 56일로 가장 짝은 저장기간을 보였다. 생체중 감소는 여름재배 처리구에서 가장 컸다. 포장내 이산화탄소와 에틸렌 농도는 가을과 겨울에 재배한 처리구는 낮았고 서로 유사한 경향을 보였으나 여름재배 처리구는 수확 당시 높은 포장열과 이로 인한 저장 중 높은 호흡으로 이보다 $2{\sim}3$배 높았다. 이러한 결과는 여름재배 처리구가 가을과 겨울에 재배한 처리구에 비해 경도, 엽록소, 비타민 C함량 등의 품질이 단기간 내에 현저히 감소를 초래하였다. 이상의 결과로 보면 수확시기별로 겨울에 수확한 파슬리의 저장성이 가장 우수하였고 파슬리 MA저장시 장기저장을 위해서는 수확직후 포장열 제거가 매우 중요하다는 것을 알 수 있었다.

• Mycobiology
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• 제42권1호
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• pp.41-45
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• 2014

The effectiveness of three kinds of enzymes (chitinase, ${\beta}$-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the ${\beta}$-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the ${\beta}$-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.

• KIEAE Journal
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• 제13권2호
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• pp.61-66
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• 2013

This study is to eliminate the need for conventional high density plant factory's artificial light source such as LED to reduce the initial investment of the light source installation as well as the operation cost. Use of solar light could enhance the quality of the vegetables similar to those grown in the natural environment. Provision of solar light into the multilayer vegetable cultivation facilities and collecting maximum and sustainable sunlight without too much loss by tracing solar path and properly distributing it through careful control during daytime are crucial for realizing the investigated rooftop light shelf system for multi-layered vegetable cultivation. In this study, we developed an innovative way of effectively allocating sunlight inside even to otherwise shaded zone of a multi-layer vegetable cultivation facility. To prove the effectiveness of the system's sunlight collection and distribution capability, both simulation and experiment in Daejeon are performed and the outcome is analyzed.

• 한국환경과학회지
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• 제33권5호
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• pp.323-332
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• 2024

Circulating hydroponic cultivation has the advantage of reducing soil and water pollution problems caused by discharge of fertilizer components because the nutrient solution is reused. However, cyclic hydroponic cultivation has a low biological buffering capacity and can cause outbreaks of infectious root pathogens. Therefore, it is necessary to develop technologies or disinfection systems to control them. This study used dielectric barrier discharge plasma, which generates various persistent oxidants, to treat Fusarium oxysporum f. sp., a pathogen that causes wilt disease. Batch and intermittent continuous inactivation experiments were conducted, and the results showed that the total residual oxidant was persistent in intermittent plasma treatment at intervals of 2-3 days, and F. oxysporum was treated efficiently. Intermittent plasma treatment did not inhibit the growth of tomatoes.

• 농약과학회지
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• 제19권3호
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• pp.287-294
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• 2015

국내 딸기 재배지에서 세균모무늬병은 2010년 최초 보고되었다. 딸기 세균모무늬병은 검역 병원균으로 분류되어 있어 수출 딸기의 품질저하와 수출통관에 문제를 야기시키기로 있는 실정이다. 본 연구는 딸기의 육묘기와 재배기에서 세균모무늬병 방제에 적합한 살세균제를 선발하는 것에 목적이 있다. 시험된 약제 중 육묘기에는 Oxolinic acid 방제가가 97.2% 나타내었으며, 재배기에는 Validamycin A의 방제가가 93.3% 나타내었다. 토양소독이 병행되지 않은 포장에서는 재배기 Validamycin A의 방제가가 80%로 감소되었다. 따라서, 토양소독과 항세균제 처방에 의한 딸기 세균모무늬병의 방제가 효과적인 것으로 판명되었다.