• Biomedical Science Letters
• /
• v.11 no.1
• /
• pp.63-69
• /
• 2005

We investigated the changes of fibrinolytic and antioxidative activities of wild or cultivated Codonopsis lanceolata, which were packed in woven polypropylene (WP) film or low density polyethylene (LDPE) film and stored for 0, 15, and 30 days at refrigerated $(2\~4^{\circ}C)$ or room $(l8\~20^{\circ}C)$ temperature (So we have 16 samples, and wrote them as the abbreviated words such as W-WP-RE-15, W-WP-RO-15, W-LDPE-RE-15, W-LDPE-RO-15, W-WP-RE-30, W-WP-RO-30, W-LDPE-RE-30, W-LDPE-RO-30, C-WP-RE-15, C-WP-RO-15, C-LDPE-RE-15, C-LDPE-RO-15, C-WP-RE-30, C-WP-RO-30, C-LDPE-RE-30, C-LDPE-RO-30). Fibrinolytic activity of fresh Codonopsis lanceolata cultivated in the mountain or field were 0.8 unit (plasmin unit/ml) or 7.3 units, respectively. In descending order, the activities of wild Codonopsis lanceolata stored for 15 days were as followed; LDPE-RE (0.70 unit), WP-RO (0.52 unit), WP-RE (0.45 unit), and LDPE-RO (0.30 unit). After 30 days, fibrinolytic activities of them decreased to 0.47 unit (LDPE-RE), 0.28 unit (WP-RO), 0.21 unit (WP-RE), and 0.30 unit (LDPE-RO). Considering from the point of fibrinolytic activity, the optimal storage condition of wild Codonopsis lanceolata was packing with LDPE film and storing at $4^{\circ}C$. The change of fibrinolytic activities of Codonopsis lanceolata cultivated in the field revealed the similar trend as wild samples, but the fibrinolytic activities of 30 days-stored samples were maintained better than the wild Codonopsis lanceolata. Fibinolytic activity of wild Codonopsis lancealata was increased by heating for 5 min at $100^{\circ}C$ and decreased by addition of NaCl. Antioxidative activities of Codonopsis lanceolata were also compared from the electron donating activity. Fresh Codonopsis lanceolata had about $70\%$ of electron donating activity. Independent of cultivation area, electron donating activity dropped to $19\~74\%$ (wild Codonopsis lanceolata) and $27\~59\%$ (cultivated Codonopsis lanceolata) during 15 days storage. But after 30 days storage, we obtained the unexpected results, which meant that the activities were higher activities than 15 days-stored samples or even though the fresh samples. In general, Codonopsis lanceolata could maintain antioxidative activities most strongly with LDPE film and chilled condition.

• Microbiology and Biotechnology Letters
• /
• v.29 no.3
• /
• pp.142-148
• /
• 2001

An indigenous antagonistic bacterium Bacillus sp. 7079 was isolated from a local soil sampled at Kyongju area in Korea . The strain has strong antagonistic ability which was originated from multifunctional mechanisms of chitinase and antibiotic and is a powerful antagonistic biocontrol agent against red-pepper rotting fungus Phytophthora capsici and Wilt fungus Fusarium oxysporum. The chitinase might degrade the cell wasll for Fusarium species. The selected Bacilus sp. 7079 was identified as a Bacillus amyloliquefaciens 7079. The maximal production of the chitinase from B, amyloliquefaciens 7079 were obtained in chitin-yeast extract medium containing 0.7%, $K_2$$HPO_4$, $0.2KH_2$$PO_4$, 0.1% ($NH_4$)$_2$$SO_4$, 0.05% sodium cirate, 0.01% $MgSO_4$$7H_2$O, 0.1% yeast extract and 0.1% colloidal chitin after cultivation of 3 days at pH 7.0 and $30^{\circ}C$. The best carbon and nitrogen sources for the production of the chitinase from B amyloliquefaciens 7079 were determined to be 0.1% colloi- dal chitin and 0.15% proteose peptone NO 3 respectively, The antagonistic activity of B amyloliquefaciens 7079 was confirmed using P. capsici by in vivo pot test with red-pepper plant.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.7 no.3
• /
• pp.201-210
• /
• 2005

This study was conducted to prepare efficient and systematic measures for nature conservation and management in Beomseom Islet, and to provide for basic data to investigate the process of vegetation change in the future. The vegetation of Beomseom Islet was classified to six vegetation type; i.e., Miscanthus sinensis community, Pinus thunbergii community, Iythophyte vegetation, evergreen broad leaf community, shrub community, and Pseudosasa japonica community. The size of each vegetation type was 40,230 $m^2$ ($23.3\%$) for shrub community, 39,366 $m^2$($22.8\%$) for Iythophyte vegetation, 30,012 $m^2$ ($17.4\%$) fur Pinus thunbergii community, 29,853 $m^2$ ($17.3\%$) for Miscanthus sinensis community, 5,564 $m^2$ ($3.2\%$) for evergreen broad leaf community, and 3,325 $m^2$ ($1.9\%$) for Pseudosasa japonica community. The area of non-vegetated sea cliff Bone that composed of bare rocks is 24,246 $m^2$($14.1\%$). We estimated that these distribution patterns of vegetation were the result of various environmental factors such as the steepness of slope and shallowness of soil as well as the cultivation of exotic plants causing disruption of native vegetation.

• Journal of Bio-Environment Control
• /
• v.19 no.2
• /
• pp.82-87
• /
• 2010

To investigate effects of kind of pot for raising of seedling (single plastic pot, double plastic pot, circular peat pot, square peat pot) and planting method of the pot (3/3, 2/3, and 1/3 by the buried degree of pot height) on watermelon (Citrullus lanatus) growth, this research was conducted under plastic film house. Since planting of seedlings, number, area and photosynthesis rate of leaves, plant height and weight were superior in seedling grown in double plastic pot than those grown in the others. In fruit characteristics harvested at 12 weeks after planting, fruit height, diameter and weight were longer in double plastic pot than in the others, also fruit peel thickness and hilum diameter were thicker and shorter. Initial growth of the plant after planting with seedling grown in double plastic pot was superior in method buried the 2/3 degree of the pot height than the other methods.

• Journal of Bio-Environment Control
• /
• v.23 no.3
• /
• pp.167-173
• /
• 2014

This research was conducted to establish appropriate methods to prune tomato side stems during summer. Cherry tomatoes "Unicorn" (Monsanto Korea, Korea) were grown in the coir based growing medium, and irrigation was controlled time based system. There were three pruning treatments: 1) removing all side stems (ACUT), 2) remaining two leaves on the side stems right below any cluster (PCUT), and 3) remaining two leaves on all side stems (LEFT). Experimental results showed that the occurrence of swollen stems, a symptom of nutrient excess, was influenced by side stem pruning due to blocking of consumption of photosynthetic products. The photosynthetic rate was not different between leaves on main stem and those on side shoots. Therefore the differences in the total amounts of photosynthetic products seemed to come out from the differences in leaf areas on each treatments, influencing on fruit yield difference. The yields and harvesting rates were better in ACUT treatment when tomato plants were harvested until $5^{th}$ cluster, however tomato yield was higher in LEFT treatment when more then $5^{th}$ clusters were harvested.

• Journal of Bio-Environment Control
• /
• v.27 no.1
• /
• pp.64-70
• /
• 2018

In this study, eleven major coastal areas were selected and the climate environment and the greenhouse direction were analyzed. This research investigates the greenhouse heat loss according to the wind environment at target areas. The target areas were selected based on heated greenhouse cultivation area and wind environment standard. Temperature, wind speed, and wind direction among weather data for 30 years were collected and analyzed. The data were divided into the minimum, average, and maximum temperatures and the Meteorological Agency criteria applied to the weather and wind direction criteria. Data were collected in the range of $0{\sim}180^{\circ}$ considering the symmetry of the shape of the greenhouse. In addition, the wind direction is different for each region and the applied wind direction can be different when referring to the longitudinal direction of the greenhouse and the data are collected in the range of $0{\sim}90^{\circ}$. The results of this study are expected to be used to calculate the heating load of greenhouse installed in places wind speed high.

• Korean Journal of Soil Science and Fertilizer
• /
• v.47 no.1
• /
• pp.36-40
• /
• 2014

Korea's agricultural land is constantly being reduced. The reasons for this are due to the change of agricultural profitability and the policy conditions. The reduction of agricultural land in 2010 showed a decline trend by 14.4 % of paddy fields and 1.2% of uplands compared to areas from 2000. These reductions were mainly due to switch rice paddy fields into upland or greenhouse facility cultivation because of low profitability of rice products compared to farm products. In addition, the permit system of agricultural areas was relaxed in switching paddy fields and this accelerated the reduction of agricultural land. For this reason, more than 1% of agricultural land area has been reduced every year for last five years. Moreover, indiscreet fill and cover materials such as construction wastes were used in agricultural lands and caused land contamination which threatened foundation as sustainable agricultural lands. For these reasons, it is a desperate situation to conserve good agricultural lands. However, the standards of transported soils, filling soils and cutting soils in the Agricultural Land Act are qualitative and have a problem of causing complaints. Therefore, the following criteria (proposals) are proposed in the Agricultural Land Act; (1) Use the proper soils for crops (criterion), (2) Soil components and amounts should be proper as transported soils (range), and (3) Prohibiting usage of improper earth rocks or recycled aggregates in case of filling soils (kinds). The presented criteria (proposals) suggest following; (1) Use physio-chemically proper soils for crops (criterion), (2) In case of transported soils, i, exclude potential acid sulphate soils, ii, gravel content sould be less than 15%, and iii, Heavy metals and other contaminants should be less than the soil contamination warning limit from the Ministry of Environments, (3) In case of filling soils, 13 kinds of recycled wastes specified in the Wastes Control Act should not be used as filling soils, (4) Practice soil conservation technology in case of sloping areas, and (5) Follow proper fertilizer application standards for maturing paddy fields and uplands when cutting soils.

• Horticultural Science & Technology
• /
• v.30 no.6
• /
• pp.673-679
• /
• 2012

This study was conducted to examine the effect of artificial light source and photoperiod on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings were grown under 3 light sources, fluorescent lamp (FL, Philips Co. Ltd., the Netherlands), WL #1 (Hepas Co. Ltd., Korea), and WL #2 (FC Poibe Co., Ltd., Korea), each with 3 photoperiods, 12/12, 18/6, and 24/0 (Light/Dark). An irradiance spectrum analysis showed that FL has various peaks in the 400-700 nm range, while WL #1 and WL #2 have only one monochromatic peak at 450 and 550 nm, respectively. The greatest plant height, fresh and dry weights were obtained in the 24/0 (Light/Dark) photoperiod. The 24/0 (Light/Dark) photoperiod treatment promoted vegetative growth of the leaf area. Length of the longest root, number of leaves, fresh weight, and total anthocyanin contents were greater in FL than in either WL #1 or #2. The greatest chlorophyll fluorescence (Fv/Fm) was found in the 12/12 (Light/Dark) photoperiod with FL treatment. The energy use efficiency of the LED increased by about 35-46% as compared to FL. Results suggest a possibility of LED being used as a substitute light source for fluorescent lamp for lettuce cultivation in a plant factory system.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.2
• /
• pp.151-155
• /
• 2020

This study was conducted to determine the effects of the selection of an appropriate sowing method in on ginseng growth, and the results are as follows. SAS (semi-automatic sower), commonly used by direct seeding farms, provides fast operation speed but results in high labor cost, while (Hooper Seeder Sowing) offers fast operation speed and high germination rate, but leads to increased laborer's fatigue. Daily working area per sower was 6,611 ㎡ for a motor type, and the operation time to sow 3,305 ㎡ was 15 hours for broadcast planting, and 8 hours for SAS. The germination rate of 1-year-old ginseng according to direct seeding was 69.1% for the treatment plot under broadcast planting, followed by 66.4% and 67.3% for manual sower and hoop sower, respectively, and the germination rate of SAS was the lowest at 64.2%. In addition, there were no differences in shoot and root growth of 1-year-old ginseng among the sowing methods. Therefore, the sowing method can be chosen depending on the economic size of ginseng farmers. That broadcast sowing (BS) is appropriate for small scale farmers, while motor-driven seeder sowing (MDS) for direct seeding is more suitable for large scale farmers.

• Applied Biological Chemistry
• /
• v.45 no.4
• /
• pp.185-189
• /
• 2002

Along with the worldwide rapid increase of the cultivation area and commercial production of genetically modified (GM) crops, the amount of GM grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-photphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of EPSPS transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conducted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.