• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.213-217
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• 2000

In order to select more proper plants for phytoextraction at the heavy metal polluted areas, 11 species of non-edible plants were cultivated at the cadmium(Cd) and Lead(Pb) treated soils and analyzed the content of the absorbed Cd and Pb in each part of plants. Plants include three fibers(Linum usitatissimum, Cannabis sativa, Gossypium spp.), three flowers(Calendula officinalis, Rhododendron lateritium, Portulaca grandiflora), and five trees(Pinus thunbergii, Magnolia kobus, $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla). Yield of tree species were higher than that of fiber and flower species. Cd and Pb were highly accumulated in root rather than leaves and stems. The Cd content of plants was in the order Portulaca grandiflora > Calendula officinalis > Gossypium spp. > Linum usitatissimum, Pb was Cannabis sativa > Linum usitatissimum > Fraxinus rhynchophylla. Total absorbed Cd by each plant was in the order $Populus\;nigra\;{\times}\;P.$ maximowiczii > Euonymus japonica > Rhododendron lateritium, but Pb was $Populus\;nigra\;{\times}\;P.$ maximowiczii > Rhododendron lateritium > Euonymus japonica. Total absorbed Cd and Pb contents in plants were negatively correlated with the residual Cd and Pb in the treated soils. It was estimated that $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla, and Rhododendron lateritium were the most effective species for phytoextraction in the polluted area considering yield and heavy metal uptake.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.710-717
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• 1996

To obtain the basic informations on the farm cultivation of Eleutherococcus senticosus Max., field survey of the native habitat in Mt. Deokyu such as weather, soils, vegetation and growth was carried out. The habitat of E. senticosus is situated at 127$^{\circ}$ 45'E, 35$^{\circ}$ 52' N in Mt. Deokyu where the elev-tion ranges from 1,050 to 1,300 meters above the sea level. Mean annual temperature forecasted around the habitat was 5.8$^{\circ}C$, mean maximum temperature in August, 24.6$^{\circ}C$ ; mean minimum temperature in January, -13.5$^{\circ}C$ and relative humidity during the growth periods, over 95%. To divide the area according to climatic conditions was classified cool temperature and humid rain forest zone. In another view, it belonged to deciduous broad-leaf forest zone and soil texture was sandy loam with dark brown gravels. Acidity and P$_2$O$_{5}$ content of soil represented pH 5.2～5.6 and 10ppm, respectively. The growth of E. senticosus was poor under the low light intensity(relative 20% of full sun-light) at the growing place of the habitat and the propagation was conducted by root sucker. The natural vegetation was consisted of big trees, 3 species; shrubs, 8 species and herbs, 4 species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.457-462
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• 2004

Lodging is one of the most serious problems in soybean cultivation. Therefore, improved cultural methods to reduce lodging as well as to increase photosynthetic ability should be mostly desirable to increase soybean production. The test variety was 'Hwangkeumkong' which was pinched at V7 stage. The greatest difference in canopy height between rows was shown when every other row was pinched, which also recorded the most effective reduction in lodging. The 9th leaf of soybean plants in non-pinching rows of alternative non-pinching and pinching plot showed the highest photosynthetic ability due to the greatest difference in canopy height. Although leaf area index was higher in pinched rows in average after 17 August, alternative pinching of every other row recorded the highest LAI on 5 September. Alternative pinching of every other row resulted $2\~14\%$ higher yield than non-pinching or complete pinching due to the increases of number of grains in the upper part of main stem and average grain weight of non-pinching rows and in alternative pinching.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.117-122
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• 2015

Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

• Biomedical Science Letters
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• v.11 no.1
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• pp.63-69
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• 2005

We investigated the changes of fibrinolytic and antioxidative activities of wild or cultivated Codonopsis lanceolata, which were packed in woven polypropylene (WP) film or low density polyethylene (LDPE) film and stored for 0, 15, and 30 days at refrigerated $(2\~4^{\circ}C)$ or room $(l8\~20^{\circ}C)$ temperature (So we have 16 samples, and wrote them as the abbreviated words such as W-WP-RE-15, W-WP-RO-15, W-LDPE-RE-15, W-LDPE-RO-15, W-WP-RE-30, W-WP-RO-30, W-LDPE-RE-30, W-LDPE-RO-30, C-WP-RE-15, C-WP-RO-15, C-LDPE-RE-15, C-LDPE-RO-15, C-WP-RE-30, C-WP-RO-30, C-LDPE-RE-30, C-LDPE-RO-30). Fibrinolytic activity of fresh Codonopsis lanceolata cultivated in the mountain or field were 0.8 unit (plasmin unit/ml) or 7.3 units, respectively. In descending order, the activities of wild Codonopsis lanceolata stored for 15 days were as followed; LDPE-RE (0.70 unit), WP-RO (0.52 unit), WP-RE (0.45 unit), and LDPE-RO (0.30 unit). After 30 days, fibrinolytic activities of them decreased to 0.47 unit (LDPE-RE), 0.28 unit (WP-RO), 0.21 unit (WP-RE), and 0.30 unit (LDPE-RO). Considering from the point of fibrinolytic activity, the optimal storage condition of wild Codonopsis lanceolata was packing with LDPE film and storing at $4^{\circ}C$. The change of fibrinolytic activities of Codonopsis lanceolata cultivated in the field revealed the similar trend as wild samples, but the fibrinolytic activities of 30 days-stored samples were maintained better than the wild Codonopsis lanceolata. Fibinolytic activity of wild Codonopsis lancealata was increased by heating for 5 min at $100^{\circ}C$ and decreased by addition of NaCl. Antioxidative activities of Codonopsis lanceolata were also compared from the electron donating activity. Fresh Codonopsis lanceolata had about $70\%$ of electron donating activity. Independent of cultivation area, electron donating activity dropped to $19\~74\%$ (wild Codonopsis lanceolata) and $27\~59\%$ (cultivated Codonopsis lanceolata) during 15 days storage. But after 30 days storage, we obtained the unexpected results, which meant that the activities were higher activities than 15 days-stored samples or even though the fresh samples. In general, Codonopsis lanceolata could maintain antioxidative activities most strongly with LDPE film and chilled condition.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.142-148
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• 2001

An indigenous antagonistic bacterium Bacillus sp. 7079 was isolated from a local soil sampled at Kyongju area in Korea . The strain has strong antagonistic ability which was originated from multifunctional mechanisms of chitinase and antibiotic and is a powerful antagonistic biocontrol agent against red-pepper rotting fungus Phytophthora capsici and Wilt fungus Fusarium oxysporum. The chitinase might degrade the cell wasll for Fusarium species. The selected Bacilus sp. 7079 was identified as a Bacillus amyloliquefaciens 7079. The maximal production of the chitinase from B, amyloliquefaciens 7079 were obtained in chitin-yeast extract medium containing 0.7%, $K_2$$HPO_4$, $0.2KH_2$$PO_4$, 0.1% ($NH_4$)$_2$$SO_4$, 0.05% sodium cirate, 0.01% $MgSO_4$$7H_2$O, 0.1% yeast extract and 0.1% colloidal chitin after cultivation of 3 days at pH 7.0 and $30^{\circ}C$. The best carbon and nitrogen sources for the production of the chitinase from B amyloliquefaciens 7079 were determined to be 0.1% colloi- dal chitin and 0.15% proteose peptone NO 3 respectively, The antagonistic activity of B amyloliquefaciens 7079 was confirmed using P. capsici by in vivo pot test with red-pepper plant.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.3
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• pp.201-210
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• 2005

This study was conducted to prepare efficient and systematic measures for nature conservation and management in Beomseom Islet, and to provide for basic data to investigate the process of vegetation change in the future. The vegetation of Beomseom Islet was classified to six vegetation type; i.e., Miscanthus sinensis community, Pinus thunbergii community, Iythophyte vegetation, evergreen broad leaf community, shrub community, and Pseudosasa japonica community. The size of each vegetation type was 40,230 $m^2$ ($23.3\%$) for shrub community, 39,366 $m^2$($22.8\%$) for Iythophyte vegetation, 30,012 $m^2$ ($17.4\%$) fur Pinus thunbergii community, 29,853 $m^2$ ($17.3\%$) for Miscanthus sinensis community, 5,564 $m^2$ ($3.2\%$) for evergreen broad leaf community, and 3,325 $m^2$ ($1.9\%$) for Pseudosasa japonica community. The area of non-vegetated sea cliff Bone that composed of bare rocks is 24,246 $m^2$($14.1\%$). We estimated that these distribution patterns of vegetation were the result of various environmental factors such as the steepness of slope and shallowness of soil as well as the cultivation of exotic plants causing disruption of native vegetation.

• Journal of Bio-Environment Control
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• v.19 no.2
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• pp.82-87
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• 2010

To investigate effects of kind of pot for raising of seedling (single plastic pot, double plastic pot, circular peat pot, square peat pot) and planting method of the pot (3/3, 2/3, and 1/3 by the buried degree of pot height) on watermelon (Citrullus lanatus) growth, this research was conducted under plastic film house. Since planting of seedlings, number, area and photosynthesis rate of leaves, plant height and weight were superior in seedling grown in double plastic pot than those grown in the others. In fruit characteristics harvested at 12 weeks after planting, fruit height, diameter and weight were longer in double plastic pot than in the others, also fruit peel thickness and hilum diameter were thicker and shorter. Initial growth of the plant after planting with seedling grown in double plastic pot was superior in method buried the 2/3 degree of the pot height than the other methods.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.167-173
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• 2014

This research was conducted to establish appropriate methods to prune tomato side stems during summer. Cherry tomatoes "Unicorn" (Monsanto Korea, Korea) were grown in the coir based growing medium, and irrigation was controlled time based system. There were three pruning treatments: 1) removing all side stems (ACUT), 2) remaining two leaves on the side stems right below any cluster (PCUT), and 3) remaining two leaves on all side stems (LEFT). Experimental results showed that the occurrence of swollen stems, a symptom of nutrient excess, was influenced by side stem pruning due to blocking of consumption of photosynthetic products. The photosynthetic rate was not different between leaves on main stem and those on side shoots. Therefore the differences in the total amounts of photosynthetic products seemed to come out from the differences in leaf areas on each treatments, influencing on fruit yield difference. The yields and harvesting rates were better in ACUT treatment when tomato plants were harvested until $5^{th}$ cluster, however tomato yield was higher in LEFT treatment when more then $5^{th}$ clusters were harvested.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.64-70
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• 2018

In this study, eleven major coastal areas were selected and the climate environment and the greenhouse direction were analyzed. This research investigates the greenhouse heat loss according to the wind environment at target areas. The target areas were selected based on heated greenhouse cultivation area and wind environment standard. Temperature, wind speed, and wind direction among weather data for 30 years were collected and analyzed. The data were divided into the minimum, average, and maximum temperatures and the Meteorological Agency criteria applied to the weather and wind direction criteria. Data were collected in the range of $0{\sim}180^{\circ}$ considering the symmetry of the shape of the greenhouse. In addition, the wind direction is different for each region and the applied wind direction can be different when referring to the longitudinal direction of the greenhouse and the data are collected in the range of $0{\sim}90^{\circ}$. The results of this study are expected to be used to calculate the heating load of greenhouse installed in places wind speed high.