• 한국환경농학회지
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• 제21권1호
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• pp.24-30
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• 2002

본 실험에서는 음지식물인 인삼의 재배에 필요한 차광조건이 토양 중 Procymidone의 잔류성에 미치는 영향을 연구하였다. 이를 위해서 몇 가지 차광재료들을 사용하여 여러 가지 차광조건을 만들고서, 각 차광조건에서의 토양 중 Procymidone의 잔류성을 조사하였다. 여러 차광재료들을 인삼포장에 가설하여 만든 다양한 차광조건하에서 토양 중 Procymidone의 반감기는 차광재료에 따라 큰 차이를 보였으며, 혼합일복 (161일)>유리 (76일)>청색차광망 (39일)$\simeq$흑색차광망 (36일)>볏짚일복 (21일)>노지 (7일) 순이었고, 이러한 토양 중 잔류성의 변화는 각 차광재료들의 누수율과 깊은 관련이 있는 것으로 해석할 수 있었다. 이러한 결과로부터 인삼재배를 위한 차광시설의 존재는 토양 중 Procymidone의 잔류성을 현저하게 증가시킨다는 사실을 알 수 있으며 농약의 토양 중 잔류성을 감소시키는 측면에서만 고려할 때, 강우시 가능한한 누수율을 증대시키는 차광재료들을 사용함이 바람직하다고 생각된다. 토양 중 Prorymidone의 잔류반감기는 차팡재료들에 따라 큰 차이를 보였으며, 이는 각 차광재료들의 누수율과 깊은 관련이 있는 것으로 해석할 수 있었다.

• 한국미생물·생명공학회지
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• 제6권2호
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• pp.75-80
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• 1978

효모의 균체생산에 관한 연구중 glutathione 함량이 다량 축적되는 효모를 검색한 결과, Rhodotorula glutinis 균주가 높은 균체량과 g1utathione 을 함육하였다. 이 균주에 대한 배양시간에 따른 glutathione의 함량증가를 검토한 결과 pH 6.0, 3$0^{\circ}C$에서 왕복진탕배양으로 48시간 배양하였을 때 가장 좋은 결과를 보였다. Glutathione 함량 증가를 보기 위해 기본배지에 아미노산을 첨가하여 glutathione 함량을 측정한 결과, 명아미노산의 최적농도는 0.7%, 최적 시기는 배양 후 36시간째 였다. Glutathiohe의 구성아미노산인 glutamic acid, cysteine, glycine을 조합하여 첨가할 때 219$\mu\textrm{g}$/ml으로 무첨가 배지의 73$\mu\textrm{g}$/ml에 비해 약 3배의 증가를 보였다.

• 한국환경교육학회지:환경교육
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• 제16권2호
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• pp.1-14
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• 2003

This study aims to review the present condition of human resources development in environment technology area and to explore the direction of human resources development plans, based on the results of a survey targeting experts in this area. Related papers & literatures are referred and a survey for experts' opinions are carried out. The survey was oriented to the importance and effectiveness of policies designed by Korea Ministry of environment. The main findings of this study are as follows; firstly, an emphasis should be put on generating professionals in charge of creating domestic demand for environmental purposes for promotion to make excellent environmental techniques being of practical use and help them go into businesses was turned out crucial; secondly, it is advised to upgrade the level of qualifications of environmental technicians by reforming into a graduate schoolcentered educational system on a human resource-rearing phase; thirdly, retraining should be provided by law to ensure the enhancement of re-educating the existing environmental technicians; fourth, efforts should be made to promptly develope new techniques and unify declining traditional items, deterring national technical qualification system oriented toward cultivating superintendents in the environment field.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.206-215
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• 2005

Polyhydroxyalkanoates (PHAs) are homo or hetero polyesters of (R)-hydroxyalkanoates accumulated in various microorganisms under growth-limiting condition in the presence of excess carbon source. They have been suggested as biodegradable substitutes for chemically synthesized polymers. Recombinant Escherichia coli is one of the promising host strains for the economical production of PHAs, and has been extensively investigated for the process development. The heterologous PHA biosynthetic pathways have been established through the metabolic engineering and inherent metabolic pathways of E. coli have been redirected to supply PHA precursors. Fermentation strategies for cultivating these recombinant E. coli strains have also been developed for the efficient production of PHAs. Nowadays, short-chain-length (SCL) PHAs are being re-invited due to its improved mechanical properties and possible applications in the biomedical area. In this article, recent advances in the development of metabolically engineered E. coli strains for the enhanced production of SCL-PHAs are reviewed. Also, medical applications of SCL-PHAs are discussed.

• 식물병연구
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• 제8권2호
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• pp.78-83
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• 2002

Tobacco diseases have not been recorded until 1900s in Korea, where tobacco plants were introduced at early 1700s. Practical researches on the disease have been conducted since mid 1960s. Major ten tobacco diseases were mosaic caused by tobacco mosaic virus·potato virus Y·cucumber mosaic virus, bacterial wilt, hollow stalk, wild fire caused by angular leaf spot strain, black shank, brown spot, powdery mildew and fusarium wilt. But their annual occurrences were varied according to changes of tobacco varieties and their cultivating practices. As no useful chemicals, several biological tactics have been developed to control the viral or bacterial diseases that give significant economic damages on sustainable crop yield, but not practicable to field farming condition yet. Transgenic tobacco plants containing foreign disease resistant genes have been developed by current bio-technology, but not released to farmers yet. Though some disease-resistant tobacco varieties have been developed by the conventional breeding technology and currently used by farmers, their disease controlling efficacy have been diminished by occurrence of the new strain or race. Future research on tobacco diseases has been focused on technical development to produce high quality tobacco with less production cost, which leads Korean tobacco industry to keep its competence against foreign industry and decreasing overall market.

• 인문언어
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• 제5권
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• pp.127-163
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• 2003

This thesis analyzes the Chapter "Hoyeonjigi(浩然之氣)" of Mencius(孟子), using text linguistics theory and reading theory of Korean. In this process the model of macro-structure #1∼5 are presented, according to Vandijk′s rules of macro-structure; Auslassen, Selektierne, Generalisieren, Konstruieren odor Integrieren. As a result, this certifies; First, macro-structure could make arbitrarily a several steps of macro-structure by types of text or purpose of analysis. Second, macro-structure applies various cognitive mechanisms of outer world as well as inner world. Third, a text with profound symbolism could be figured as a two-or threefolded symbolic structure. At the same time, macro-structure enables the clearer analysis of the content of the Chapter to verify the following; first, Hoyeinjigi itself is the best measure of developing "Imperturbable Mind(不動心)" Second, benevolence-righteousness(仁義) and wisdom(智) would be reached by cultivating Hoyeonjigi. Third, Mencius′ own view of language is well expressed in "Jieon(知言)", which is not only a condition for Imperturbable Mind, but also the Oriental view of language focused especially on listening in terms of language usage, not language analysis. This Mencius′ view of language has a thread connection with that of Oriental′s.

• 한국식품조리과학회지
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• 제17권5호
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• pp.510-516
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• 2001

Hydroponic cultivation is a technology of raising crops without use of soil. Generally farmers use the method of DFT(deep flow technology)to grow leafy or fruity vegetables; however, systematic and scientific researches are insufficient on this matter. This study investigated the possibility of cultivating Chinese cabbage steadily year long by using the method of DFT. Chinese cabbage was cultivated hydroponically with and without Ge addition, used to prepare kimchi, and the chemical and microbiological characteristics of kimchi were compared. The basic hydroponic cultivation condition was as follows: 30 days after seeding, the raised seeds were moved to a hydroponic bed and given underground water for 3 days so the roots grow normally Standard nutrient solution was provided and the early electric conductivity concentration was maintained between 1.5∼2.5 thickness. The temperature of the solution was maintained between 10 ∼25$^{\circ}C$ to allow the growth of Chinese cabbage. When soil-cultivated, organically cultivated and hydroponically cultivated Chinese cabbages were compared, hydroponically cultivated cabbages were smaller in size and showed less ability to build up and fold leaves into a head, but showed better quality than organically cultivated cabbages. The contents of protein and fat showed no significant differences. The contents of water. Ca, P, Fe, Vitamin A and Niacin were higher in control and Ge-added cabbages compared with soil-grown cabbage. There was no difference between soil-cultivated Chinese cabbage kimchi and hydroponically cultivated Chinese cabbage kimchi.

• 한국자원식물학회지
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• 제19권4호
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• pp.517-520
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• 2006

봉의꼬리에 있어서 생육은 일반상토인 모래: 마사토 : 부엽토(2:5:3)와 피트모스 : 이끼(5:5) 조합에서 많았으므로 인공상토로는 피트모스에 이끼를 덮은 조합이 유리한 것으로 나타났다. 도깨비고비의 경우에는 전체적으로 배양토 적응성이 높게 나타났으며 역시 피트모스 : 펄라이트(7:3) 조합에서 생육량이 가장 많았다. 그러나 바크와 톱밥이 들어간 배양토에서는 생육이 저조하였다. 부싯깃고사리의 경우 초장과 엽수의 생육으로 볼 때 모래 : 마사토 : 부엽(2:5:3) 조합이 가장 크고 많았으며 그 다음이 피트모스 : 펄라이트(7:3, 5:5) 조합 순이었다.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.939-946
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• 2012

Antibiotic production with Streptomyces sindenensis MTCC 8122 was optimized under submerged fermentation conditions by artificial neural network (ANN) coupled with genetic algorithm (GA) and Nelder-Mead downhill simplex (NMDS). Feed forward back-propagation ANN was trained to establish the mathematical relationship among the medium components and length of incubation period for achieving maximum antibiotic yield. The optimization strategy involved growing the culture with varying concentrations of various medium components for different incubation periods. Under non-optimized condition, antibiotic production was found to be $95{\mu}g/ml$, which nearly doubled ($176{\mu}g/ml$) with the ANN-GA optimization. ANN-NMDS optimization was found to be more efficacious, and maximum antibiotic production ($197{\mu}g/ml$) was obtained by cultivating the cells with (g/l) fructose 2.7602, $MgSO_4$ 1.2369, $(NH_4)_2PO_4$ 0.2742, DL-threonine 3.069%, and soyabean meal 1.952%, for 9.8531 days of incubation, which was roughly 12% higher than the yield obtained by ANN coupled with GA under the same conditions.

• Mycobiology
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• 제35권4호
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• pp.226-229
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• 2007

To produce fruiting bodies of Oudemansiella mucida, porcelain fungus, on the oak sawdust medium, additives suitable for the mycelial growth and fruiting body formation were screened. In general, the mycelial growth of the three strains of O. mucida used in this study have been good on oak sawdust mixed rice bran of $20{\sim}30%$. The mycelia incubated in potato dextrose broth for 7 days were inoculated on oak sawdust medium supplemented with various ratios of rice bran and incubated for 30 days at $25^{\circ}C$ in the dark condition until the mycelia of O. mucida fully colonized the media from top to bottom. Then, top surface of the media in the bottles were horizontally scratched with a spatula and filled with tap water for 3 hours. To induce the primordial formation of O. mucida, the bottles were transferred to the mushroom cultivating room under 12 hrs of light (350 lux) and dark condition with relative humidity of 95% at $17^{\circ}C$. The primordia of O. mucida were formed on the surface of oak sawdust media after 7 days of incubation. The mature fruiting bodies were observed 5 days after primordial formation. The fruiting bodies O. mucida were formed on oak sawdust medium mixed with 5 to 30% rice bran. However, abundant fruiting-bodies of O. mucida were produced in oak sawdust medium supplemented with 20% rice bran. This is the first report associated with an artificial fruiting body production of O. mucida in Korea.